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The map label for this gene is mpl [H]

Identifier: 220918632

GI number: 220918632

Start: 3956911

End: 3958335

Strand: Reverse

Name: mpl [H]

Synonym: A2cp1_3540

Alternate gene names: 220918632

Gene position: 3958335-3956911 (Counterclockwise)

Preceding gene: 220918633

Following gene: 220918629

Centisome position: 78.71

GC content: 74.32

Gene sequence:

>1425_bases
GTGATCGACTGGAAGAGCGTGAAGCGCATCCACCTCATCGGCGTGGCGGGCACCGGCATGGGCTCGTTCGCGGGCATGCT
GAAGGCCGCCGGCTACCAGGTCACCGGCTCGGACGAGAACGTCTACCCGCCCATGTCCACCCAGCTCGAGCGCTGGGGCA
TCGAGGCGATGACGCCGTACGCGGCCGCGAACCTCGACCGCGCCCGGCCCGACCTGGTGGTGGTGGGAAACGTGGTGCGG
TCGGTGAACGCGGAGGCGGCGGCGGTGCGCGAGCGGGGCCTGCCGCACGTGTCCTTCCCGGCGGCGCTGGGCGAGCTGTT
CATCGGGCCCCGCCACGGCGTGGTGGTGGTGGGCACGCACGGCAAGACCACCACCTCCGCGATGATGGGCTTCGTGCTGC
ACCACGCCGGCCGCGATCCGTCCTTCCTGGTCGGCGGCGTGACCCGCGACTTCGACTCCAACTTCCGCCTGGGGAGCGGT
CCGCACTTCGTCGTCGAAGGCGACGAGTACGACACCGCCTACTTCGACAAGGGGCCGAAGTTCCTCCACTACCGCCCGCG
CACCGCGATCTTCACGAGCTGCGAGCTGGACCACGCCGACATCTACCGGGACGAGGCGCACTACGAGTCGGCGTTCGAGC
GGTTCGCCGACCTGCTCCCGCCGGACGGCTTCCTGGCCACCTGCGCCGCGTACGAGAGCGTGCGCCGCATCTCCGCCCGG
GCGCGGTGCCGGATCGAGACCTACGGCGTCGACGTCGCCGGCGCCGACTGGGAGGCGCGCGGCCTGTCGCTCGGGCCCGA
CGGCGCCCGCTTCACGCTGGTGCGCCGGGGCGCGCCGCTCGCCGAGGTCCACCTGCCGGTGGGCGGGGCGCACAACGTGG
AGAACGCGCTCGGGGTCGCCGCCGCGGCCACCGCGCTCGGGCTCTCCCCCGCCGAGATCGCCGCCGGGCTGGGCGCGTTC
CACGGCGTGCGCCGCCGGCAGGAGGTGCGCGGCACGGCGGGCGGGGTGACGGTGATCGACGACTTCGCGCACCACCCGCG
CGCGGTGAAGAAGACGCTGGCGGCGGTGCGGGGGAGCTTCCCCGGCGCCCGCATGCTGGCCGCGTTCGAGCCGCGCTCCA
ACACCAGCCGCCGCAACCTGCACCAGGCCGACTACTCCGATCCGGCCACCTGGTCCGGCGCGGCCGAGGTGTTCATCCTG
CGCCCGGCCCCGACCGACCGCGTCCCGGAGGCGGAGCGGCTGGACGTGGACGCGGTGGTGCGCGCGCTCGCGGCGCACGG
GCTGCCCGCGCGCGTGTTCGACGCGGTGGATCCCCTGGTCGAGGCCATCGCGGCCGACGCGCGCCCCGGCGACGTAGCGG
TCGCCATGTCGAACGGCGCCTTCGGCGGAATCTGGGGCAAGCTGCTCGCGCGCGTCGGGCGCTGA

Upstream 100 bases:

>100_bases
TTCCACGACGCGGTGGCGGAGGCGCTGGGGATCGGGTAGGCCGTGCATCTCGCCGCCGCCCCGCGCCCGCGCGCGCGGCC
GGAGCGGCACCGGAGCGAAC

Downstream 100 bases:

>100_bases
GCGGCGCTGAGCGGCGCCCCCCCCGCTCGCGGCCCGCGAGAAAGCGAGCGCAGCGAGCCCGCCGGGGCGACGCCGCGCTT
GCGGCGAAGCCGCTCGCGGC

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MIDWKSVKRIHLIGVAGTGMGSFAGMLKAAGYQVTGSDENVYPPMSTQLERWGIEAMTPYAAANLDRARPDLVVVGNVVR
SVNAEAAAVRERGLPHVSFPAALGELFIGPRHGVVVVGTHGKTTTSAMMGFVLHHAGRDPSFLVGGVTRDFDSNFRLGSG
PHFVVEGDEYDTAYFDKGPKFLHYRPRTAIFTSCELDHADIYRDEAHYESAFERFADLLPPDGFLATCAAYESVRRISAR
ARCRIETYGVDVAGADWEARGLSLGPDGARFTLVRRGAPLAEVHLPVGGAHNVENALGVAAAATALGLSPAEIAAGLGAF
HGVRRRQEVRGTAGGVTVIDDFAHHPRAVKKTLAAVRGSFPGARMLAAFEPRSNTSRRNLHQADYSDPATWSGAAEVFIL
RPAPTDRVPEAERLDVDAVVRALAAHGLPARVFDAVDPLVEAIAADARPGDVAVAMSNGAFGGIWGKLLARVGR

Sequences:

>Translated_474_residues
MIDWKSVKRIHLIGVAGTGMGSFAGMLKAAGYQVTGSDENVYPPMSTQLERWGIEAMTPYAAANLDRARPDLVVVGNVVR
SVNAEAAAVRERGLPHVSFPAALGELFIGPRHGVVVVGTHGKTTTSAMMGFVLHHAGRDPSFLVGGVTRDFDSNFRLGSG
PHFVVEGDEYDTAYFDKGPKFLHYRPRTAIFTSCELDHADIYRDEAHYESAFERFADLLPPDGFLATCAAYESVRRISAR
ARCRIETYGVDVAGADWEARGLSLGPDGARFTLVRRGAPLAEVHLPVGGAHNVENALGVAAAATALGLSPAEIAAGLGAF
HGVRRRQEVRGTAGGVTVIDDFAHHPRAVKKTLAAVRGSFPGARMLAAFEPRSNTSRRNLHQADYSDPATWSGAAEVFIL
RPAPTDRVPEAERLDVDAVVRALAAHGLPARVFDAVDPLVEAIAADARPGDVAVAMSNGAFGGIWGKLLARVGR
>Mature_474_residues
MIDWKSVKRIHLIGVAGTGMGSFAGMLKAAGYQVTGSDENVYPPMSTQLERWGIEAMTPYAAANLDRARPDLVVVGNVVR
SVNAEAAAVRERGLPHVSFPAALGELFIGPRHGVVVVGTHGKTTTSAMMGFVLHHAGRDPSFLVGGVTRDFDSNFRLGSG
PHFVVEGDEYDTAYFDKGPKFLHYRPRTAIFTSCELDHADIYRDEAHYESAFERFADLLPPDGFLATCAAYESVRRISAR
ARCRIETYGVDVAGADWEARGLSLGPDGARFTLVRRGAPLAEVHLPVGGAHNVENALGVAAAATALGLSPAEIAAGLGAF
HGVRRRQEVRGTAGGVTVIDDFAHHPRAVKKTLAAVRGSFPGARMLAAFEPRSNTSRRNLHQADYSDPATWSGAAEVFIL
RPAPTDRVPEAERLDVDAVVRALAAHGLPARVFDAVDPLVEAIAADARPGDVAVAMSNGAFGGIWGKLLARVGR

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=462, Percent_Identity=40.6926406926407, Blast_Score=321, Evalue=8e-89,
Organism=Escherichia coli, GI1786279, Length=461, Percent_Identity=31.4533622559653, Blast_Score=154, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50516; Mature: 50516

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDWKSVKRIHLIGVAGTGMGSFAGMLKAAGYQVTGSDENVYPPMSTQLERWGIEAMTPY
CCCCCCCCEEEEEEEECCCCHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHCCCHHCCCH
AAANLDRARPDLVVVGNVVRSVNAEAAAVRERGLPHVSFPAALGELFIGPRHGVVVVGTH
HHCCCCCCCCCEEEEEHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEECC
GKTTTSAMMGFVLHHAGRDPSFLVGGVTRDFDSNFRLGSGPHFVVEGDEYDTAYFDKGPK
CCHHHHHHHHHHHHHCCCCCCEEEECEEECCCCCCCCCCCCEEEEECCCCCCHHHCCCCC
FLHYRPRTAIFTSCELDHADIYRDEAHYESAFERFADLLPPDGFLATCAAYESVRRISAR
EEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ARCRIETYGVDVAGADWEARGLSLGPDGARFTLVRRGAPLAEVHLPVGGAHNVENALGVA
HEEEEEEECCEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHH
AAATALGLSPAEIAAGLGAFHGVRRRQEVRGTAGGVTVIDDFAHHPRAVKKTLAAVRGSF
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHHHHHHCCC
PGARMLAAFEPRSNTSRRNLHQADYSDPATWSGAAEVFILRPAPTDRVPEAERLDVDAVV
CCCEEEEEECCCCCCCHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCHHHHH
RALAAHGLPARVFDAVDPLVEAIAADARPGDVAVAMSNGAFGGIWGKLLARVGR
HHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIDWKSVKRIHLIGVAGTGMGSFAGMLKAAGYQVTGSDENVYPPMSTQLERWGIEAMTPY
CCCCCCCCEEEEEEEECCCCHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHCCCHHCCCH
AAANLDRARPDLVVVGNVVRSVNAEAAAVRERGLPHVSFPAALGELFIGPRHGVVVVGTH
HHCCCCCCCCCEEEEEHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEECC
GKTTTSAMMGFVLHHAGRDPSFLVGGVTRDFDSNFRLGSGPHFVVEGDEYDTAYFDKGPK
CCHHHHHHHHHHHHHCCCCCCEEEECEEECCCCCCCCCCCCEEEEECCCCCCHHHCCCCC
FLHYRPRTAIFTSCELDHADIYRDEAHYESAFERFADLLPPDGFLATCAAYESVRRISAR
EEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ARCRIETYGVDVAGADWEARGLSLGPDGARFTLVRRGAPLAEVHLPVGGAHNVENALGVA
HEEEEEEECCEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHH
AAATALGLSPAEIAAGLGAFHGVRRRQEVRGTAGGVTVIDDFAHHPRAVKKTLAAVRGSF
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHHHHHHCCC
PGARMLAAFEPRSNTSRRNLHQADYSDPATWSGAAEVFILRPAPTDRVPEAERLDVDAVV
CCCEEEEEECCCCCCCHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCHHHHH
RALAAHGLPARVFDAVDPLVEAIAADARPGDVAVAMSNGAFGGIWGKLLARVGR
HHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]