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The map label for this gene is amiC [H]

Identifier: 220918600

GI number: 220918600

Start: 3922732

End: 3923481

Strand: Reverse

Name: amiC [H]

Synonym: A2cp1_3508

Alternate gene names: 220918600

Gene position: 3923481-3922732 (Counterclockwise)

Preceding gene: 220918601

Following gene: 220918599

Centisome position: 78.01

GC content: 76.53

Gene sequence:

>750_bases
GTGCTCGCGCACCTCGCCGCCGCGCTGATCGCGCTCTCCGCCACCCGGGGGCCGAGCTTCGTCGCCGTGATCGATCCCGG
CCACGGCGGCGAGCAGGAGGGGGCGGTCTCGCCGCGCGGCGACCGCGAGAAGGAGCTGACGCTCCAGATCGCCCGGCGCG
TGGCGGCCCGGCTGAAGCGCCTCGGCGCGAAGGCCGTGCTCACCCGCGCCGCCGACGCCTCGGTGCCGCTCGCCGCGCGC
GCCGCGCTCGCGAACGCGATCCGCGCCGACCTGTTCGTCTCCATCCACCTGAACTCGATGCCCACCGCCGAGGCGCGCCG
CGCCTCCCACGGCATCGAGACGTACTTCCTGTCGGCCGACGCGAGCGACGCCTCCGCCACGGCGGTGGCGGCCCGCGAGA
ACGCCGACCGGCTGGCCGGCGAGCCCGAGCTGGACCCGTCGGATCCGGTCGCCGCCATCCTGAGCGACCTCGAGGACACC
GCGGCGCTGCAGCAGTCGTCCCGCCTCGCGTACGCGGTGCAGGAGCGGCTGGTCCAGGCGCTCGGGGCGGAGGACCGCGG
CGTGAAGCAGGCCCCGTTCTACGTGCTCGCCGGGGCGCGCATGCCGGCGGTGCTGCTGGAGGTCGGGTTCATCTCGCACC
CGGCCGAGGGCGACCGGCTGCGCAGCGCGGCCTACCAGGAGCAGGTGGCGGGCGCCATCGTCGAGGGGATCGCCGCCTAC
CGCGCCCAGACGGTCCGGGCGAGCCGCTAG

Upstream 100 bases:

>100_bases
CCGGGCGGCCGGCGTAGCGGCGGTGGAGGTGGGAGAGGTGCTCCCCGGCGACCCGCCGCGGATCGAGCTCGTCCCCTAGC
CTCTGCTACAATCCGCGGCC

Downstream 100 bases:

>100_bases
CCCCCGCCGTCCGGGACGCCCCGTGGGCGTGGCGCGCCCGGCGCGCCCGTGCTAATCCCGCCGCCATGCGAAAGAACGGC
CGCGGCGAGCTCGACCTCCG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MLAHLAAALIALSATRGPSFVAVIDPGHGGEQEGAVSPRGDREKELTLQIARRVAARLKRLGAKAVLTRAADASVPLAAR
AALANAIRADLFVSIHLNSMPTAEARRASHGIETYFLSADASDASATAVAARENADRLAGEPELDPSDPVAAILSDLEDT
AALQQSSRLAYAVQERLVQALGAEDRGVKQAPFYVLAGARMPAVLLEVGFISHPAEGDRLRSAAYQEQVAGAIVEGIAAY
RAQTVRASR

Sequences:

>Translated_249_residues
MLAHLAAALIALSATRGPSFVAVIDPGHGGEQEGAVSPRGDREKELTLQIARRVAARLKRLGAKAVLTRAADASVPLAAR
AALANAIRADLFVSIHLNSMPTAEARRASHGIETYFLSADASDASATAVAARENADRLAGEPELDPSDPVAAILSDLEDT
AALQQSSRLAYAVQERLVQALGAEDRGVKQAPFYVLAGARMPAVLLEVGFISHPAEGDRLRSAAYQEQVAGAIVEGIAAY
RAQTVRASR
>Mature_249_residues
MLAHLAAALIALSATRGPSFVAVIDPGHGGEQEGAVSPRGDREKELTLQIARRVAARLKRLGAKAVLTRAADASVPLAAR
AALANAIRADLFVSIHLNSMPTAEARRASHGIETYFLSADASDASATAVAARENADRLAGEPELDPSDPVAAILSDLEDT
AALQQSSRLAYAVQERLVQALGAEDRGVKQAPFYVLAGARMPAVLLEVGFISHPAEGDRLRSAAYQEQVAGAIVEGIAAY
RAQTVRASR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=226, Percent_Identity=36.283185840708, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI87082163, Length=231, Percent_Identity=35.4978354978355, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1790611, Length=241, Percent_Identity=31.5352697095436, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26049; Mature: 26049

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAHLAAALIALSATRGPSFVAVIDPGHGGEQEGAVSPRGDREKELTLQIARRVAARLKR
CHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LGAKAVLTRAADASVPLAARAALANAIRADLFVSIHLNSMPTAEARRASHGIETYFLSAD
HCHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHCCCEEEEEECC
ASDASATAVAARENADRLAGEPELDPSDPVAAILSDLEDTAALQQSSRLAYAVQERLVQA
CCCCHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAEDRGVKQAPFYVLAGARMPAVLLEVGFISHPAEGDRLRSAAYQEQVAGAIVEGIAAY
HCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RAQTVRASR
HHHHHHCCC
>Mature Secondary Structure
MLAHLAAALIALSATRGPSFVAVIDPGHGGEQEGAVSPRGDREKELTLQIARRVAARLKR
CHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LGAKAVLTRAADASVPLAARAALANAIRADLFVSIHLNSMPTAEARRASHGIETYFLSAD
HCHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHCCCEEEEEECC
ASDASATAVAARENADRLAGEPELDPSDPVAAILSDLEDTAALQQSSRLAYAVQERLVQA
CCCCHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAEDRGVKQAPFYVLAGARMPAVLLEVGFISHPAEGDRLRSAAYQEQVAGAIVEGIAAY
HCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RAQTVRASR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]