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The map label for this gene is rph

Identifier: 220918599

GI number: 220918599

Start: 3921947

End: 3922666

Strand: Reverse

Name: rph

Synonym: A2cp1_3507

Alternate gene names: 220918599

Gene position: 3922666-3921947 (Counterclockwise)

Preceding gene: 220918600

Following gene: 220918598

Centisome position: 78.0

GC content: 74.03

Gene sequence:

>720_bases
ATGCGAAAGAACGGCCGCGGCGAGCTCGACCTCCGCCCCATCCTCCTCGAGCCCCGCGTCTCCAAGCACGCGGAGGGCTC
CTGCCTGGTTCGCTTCGGCGACACCCACGTGCTGTGCACCGCCTCGGTGGACGAGAAGGTCCCGCCGCACGTGTACGGCA
CCGGCGCGGGCTGGGTCACCGCCGAGTACGGCATGCTGCCGCGCTCCACGCACGAGCGCATGCAGCGCGAGGCGGCCCGC
GGGAAGCAGACCGGGCGCACCCTCGAGATCCAGCGGCTGGTCGGGCGGGCGCTGCGCGCCGCGGTGGATCTGCGCGCCAT
CGGGCCGCGCACCGTGACGCTCGACTGCGACGTGATCCAGGCCGACGGCGGCACCCGCACCGCCGCCATCACCGGCGCCT
ACGTGGCGCTCGTCCAGGCGGTGCGCGGGATCCAGAAGCGCCGGCAGCTCGCGCAGGACCCGGTGAAGCGCAGCGTGGCG
GCGGTGTCGGTGGGGATCGTCGCGGGGCAGGTGCACCTCGACCTCGACTACGACGAGGACTCCACCGCCGAGGTGGACAT
GAACGTGGTCGCGACCGGCGACGGCGCGCTGGTCGAGGTCCAGGGCACCGCCGAGGGCAAGCCGTTCCCGCGCGCCGAGC
TGGATCGGATGCTCGACGCCGCGCTGGCCGGCCTGTCGCGCCTGAAGGAGCTCCAGGAGGCGGCGCTTCGCACGCCGTGA

Upstream 100 bases:

>100_bases
CCTACCGCGCCCAGACGGTCCGGGCGAGCCGCTAGCCCCCGCCGTCCGGGACGCCCCGTGGGCGTGGCGCGCCCGGCGCG
CCCGTGCTAATCCCGCCGCC

Downstream 100 bases:

>100_bases
GCCCCGTGGATCTGCTGTTCGGCACCACGAACCCCGGGAAGCTGCGCGAGCTGCGCCGGCTGGTGGCGGGCCTGGCGGTG
CGGGTGGTCTCGCCCGACGA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRKNGRGELDLRPILLEPRVSKHAEGSCLVRFGDTHVLCTASVDEKVPPHVYGTGAGWVTAEYGMLPRSTHERMQREAAR
GKQTGRTLEIQRLVGRALRAAVDLRAIGPRTVTLDCDVIQADGGTRTAAITGAYVALVQAVRGIQKRRQLAQDPVKRSVA
AVSVGIVAGQVHLDLDYDEDSTAEVDMNVVATGDGALVEVQGTAEGKPFPRAELDRMLDAALAGLSRLKELQEAALRTP

Sequences:

>Translated_239_residues
MRKNGRGELDLRPILLEPRVSKHAEGSCLVRFGDTHVLCTASVDEKVPPHVYGTGAGWVTAEYGMLPRSTHERMQREAAR
GKQTGRTLEIQRLVGRALRAAVDLRAIGPRTVTLDCDVIQADGGTRTAAITGAYVALVQAVRGIQKRRQLAQDPVKRSVA
AVSVGIVAGQVHLDLDYDEDSTAEVDMNVVATGDGALVEVQGTAEGKPFPRAELDRMLDAALAGLSRLKELQEAALRTP
>Mature_239_residues
MRKNGRGELDLRPILLEPRVSKHAEGSCLVRFGDTHVLCTASVDEKVPPHVYGTGAGWVTAEYGMLPRSTHERMQREAAR
GKQTGRTLEIQRLVGRALRAAVDLRAIGPRTVTLDCDVIQADGGTRTAAITGAYVALVQAVRGIQKRRQLAQDPVKRSVA
AVSVGIVAGQVHLDLDYDEDSTAEVDMNVVATGDGALVEVQGTAEGKPFPRAELDRMLDAALAGLSRLKELQEAALRTP

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=64.5933014354067, Blast_Score=271, Evalue=3e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_ANAD2 (B8J5H5)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002493903.1
- ProteinModelPortal:   B8J5H5
- SMR:   B8J5H5
- GeneID:   7298089
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_3507
- HOGENOM:   HBG737187
- ProtClustDB:   PRK00173
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25709; Mature: 25709

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKNGRGELDLRPILLEPRVSKHAEGSCLVRFGDTHVLCTASVDEKVPPHVYGTGAGWVT
CCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCEEECCCCCEE
AEYGMLPRSTHERMQREAARGKQTGRTLEIQRLVGRALRAAVDLRAIGPRTVTLDCDVIQ
EECCCCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHEECCCEEEEEEEEEEE
ADGGTRTAAITGAYVALVQAVRGIQKRRQLAQDPVKRSVAAVSVGIVAGQVHLDLDYDED
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEECCCCC
STAEVDMNVVATGDGALVEVQGTAEGKPFPRAELDRMLDAALAGLSRLKELQEAALRTP
CCCEEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRKNGRGELDLRPILLEPRVSKHAEGSCLVRFGDTHVLCTASVDEKVPPHVYGTGAGWVT
CCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCEEECCCCCEE
AEYGMLPRSTHERMQREAARGKQTGRTLEIQRLVGRALRAAVDLRAIGPRTVTLDCDVIQ
EECCCCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHEECCCEEEEEEEEEEE
ADGGTRTAAITGAYVALVQAVRGIQKRRQLAQDPVKRSVAAVSVGIVAGQVHLDLDYDED
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEECCCCC
STAEVDMNVVATGDGALVEVQGTAEGKPFPRAELDRMLDAALAGLSRLKELQEAALRTP
CCCEEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA