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The map label for this gene is pgaC [H]

Identifier: 220918214

GI number: 220918214

Start: 3439981

End: 3441246

Strand: Reverse

Name: pgaC [H]

Synonym: A2cp1_3117

Alternate gene names: 220918214

Gene position: 3441246-3439981 (Counterclockwise)

Preceding gene: 220918215

Following gene: 220918213

Centisome position: 68.42

GC content: 70.54

Gene sequence:

>1266_bases
GTGACCGCCACCCTCGACAGCGCGTGGCAAGGCCTGCTGGCCTTCGTGTTCTATTACCCGCTGTTCATGTCCTTCCTGTG
GATGACCGGCGCGGCGCTGTTCTACTGGCGCTTCGAGCGGGCGGAGCGCAGCCTCGACCGGCCTCCGCCGCTCCCGCACT
GGCCGCCGGTGACCGTCCTCATCCCCTCGTACAACGAGGGGGAGAACGCCGAGGAGACGGTGAGCCACGCGCTCGCGCTC
GACTACCCGGAGTTCGAGGTGGTCGCGATCAACGACGGCAGCAGCGACGACACCGGCGCGGTGCTGGAGCGGCTGGCGGC
GCGCGACCCGCGCCTGCGGGTGGTGCACCTGGCGGAGAACCAGGGCAAGGCGATGGGGCTGCAGGCCGGCGCGCTGCTGG
CGCGGCACGAGATCCTCGTCTGCATCGACGGCGACGCCCTGCTCGACCCGAGGGCGGCGCACTGGCTGGTCCGCCACTTC
GTCGAGTCCCCCGACGTCGCGGCGGTGACCGGCAACCCGCGGATCCGCAACCGCTCCACGCTGCTCGGGCGCGTGCAGGT
CGGCGAGTTCTCGTCGATCGTCGGCATCATCAAGCGGGCGCAGCGGGTGTTCGGCAGGATCTTCACGGTCAGCGGCGTGA
TCACCGCGTTCCGCAAGTCGGCGGTGCACCAGGTGGGCTACTGGAGCGCCGACATGTTGACCGAGGACATCGACATCACC
TGGAAGCTGCAGCGCGCCGGGTGGGACGTCCGGTTCGAGCCGCGGGCGCTCACCTGGATCCTCATGCCCGAGACGCTGCG
CGGCCTGTGGAAGCAGCGGCTGCGCTGGGCCATGGGCGGCGCCCAGGTGCTCCTCAAGAACATCGACGCGGCGCTCGAGC
CGGCGCAGCGGTCGCTGTGGCCGCTGCTCGTCGAGATGGTGGTCAGCGTGGCCTGGTCGTACGCCATGGTGCTGGTCACG
GCCACCTGGCTGGTCGGTCTGGTGCTGCCGGGGGGCGTCCTCCCGGCGGTCGGATCACCCATGGGGTTGCAGGGGAGCGG
CGTCGTCCTGGGCGCCGCCTGCCTGGCCCAGTTCGCGTTCAGCACCTGGCTCGACAGCCGGTACGACCGCGGGCTGTTCC
GCAACCTCTTCTGGATGATCTGGTATCCCTTCGTATTCTGGGTGATAAATTGCGCGGCCACGGTGGTCGCCTACCCGAAG
GTCGTGGCGCGGCGCCGCGGCTCGCGGGCGCGCTGGGTCAGCCCGGACCGAGGGTTCCGCCCTTGA

Upstream 100 bases:

>100_bases
CTACCCCGACGCGCTGTTCGCCGGGCAGCCCGATCCGCGGGTCCTGAAGCCGGTGCTCGACTCGAAGCCGAACGCTCCCG
CGGTTCGCTGAGGCCGCCGC

Downstream 100 bases:

>100_bases
ATAGACCGGGGTGCCGCCACGTCCCCTTGCGGCCCCCGGGCCGGCGCAGCGGCCGGCCCGGCGGATGGAGACGGCAGGCG
GAGCAGGTGGCGCGCCGCAG

Product: N-glycosyltransferase

Products: NA

Alternate protein names: PGA synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm PGA synthesis protein PgaC; N-acetylglucosaminyltransferase PgaC [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTATLDSAWQGLLAFVFYYPLFMSFLWMTGAALFYWRFERAERSLDRPPPLPHWPPVTVLIPSYNEGENAEETVSHALAL
DYPEFEVVAINDGSSDDTGAVLERLAARDPRLRVVHLAENQGKAMGLQAGALLARHEILVCIDGDALLDPRAAHWLVRHF
VESPDVAAVTGNPRIRNRSTLLGRVQVGEFSSIVGIIKRAQRVFGRIFTVSGVITAFRKSAVHQVGYWSADMLTEDIDIT
WKLQRAGWDVRFEPRALTWILMPETLRGLWKQRLRWAMGGAQVLLKNIDAALEPAQRSLWPLLVEMVVSVAWSYAMVLVT
ATWLVGLVLPGGVLPAVGSPMGLQGSGVVLGAACLAQFAFSTWLDSRYDRGLFRNLFWMIWYPFVFWVINCAATVVAYPK
VVARRRGSRARWVSPDRGFRP

Sequences:

>Translated_421_residues
MTATLDSAWQGLLAFVFYYPLFMSFLWMTGAALFYWRFERAERSLDRPPPLPHWPPVTVLIPSYNEGENAEETVSHALAL
DYPEFEVVAINDGSSDDTGAVLERLAARDPRLRVVHLAENQGKAMGLQAGALLARHEILVCIDGDALLDPRAAHWLVRHF
VESPDVAAVTGNPRIRNRSTLLGRVQVGEFSSIVGIIKRAQRVFGRIFTVSGVITAFRKSAVHQVGYWSADMLTEDIDIT
WKLQRAGWDVRFEPRALTWILMPETLRGLWKQRLRWAMGGAQVLLKNIDAALEPAQRSLWPLLVEMVVSVAWSYAMVLVT
ATWLVGLVLPGGVLPAVGSPMGLQGSGVVLGAACLAQFAFSTWLDSRYDRGLFRNLFWMIWYPFVFWVINCAATVVAYPK
VVARRRGSRARWVSPDRGFRP
>Mature_420_residues
TATLDSAWQGLLAFVFYYPLFMSFLWMTGAALFYWRFERAERSLDRPPPLPHWPPVTVLIPSYNEGENAEETVSHALALD
YPEFEVVAINDGSSDDTGAVLERLAARDPRLRVVHLAENQGKAMGLQAGALLARHEILVCIDGDALLDPRAAHWLVRHFV
ESPDVAAVTGNPRIRNRSTLLGRVQVGEFSSIVGIIKRAQRVFGRIFTVSGVITAFRKSAVHQVGYWSADMLTEDIDITW
KLQRAGWDVRFEPRALTWILMPETLRGLWKQRLRWAMGGAQVLLKNIDAALEPAQRSLWPLLVEMVVSVAWSYAMVLVTA
TWLVGLVLPGGVLPAVGSPMGLQGSGVVLGAACLAQFAFSTWLDSRYDRGLFRNLFWMIWYPFVFWVINCAATVVAYPKV
VARRRGSRARWVSPDRGFRP

Specific function: Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N- acetylglucosamine to produce the linear homopolymer poly-beta-1,6- N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide [H]

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=418, Percent_Identity=48.8038277511962, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1786560, Length=207, Percent_Identity=25.1207729468599, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 47207; Mature: 47076

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATLDSAWQGLLAFVFYYPLFMSFLWMTGAALFYWRFERAERSLDRPPPLPHWPPVTVL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
IPSYNEGENAEETVSHALALDYPEFEVVAINDGSSDDTGAVLERLAARDPRLRVVHLAEN
ECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEECC
QGKAMGLQAGALLARHEILVCIDGDALLDPRAAHWLVRHFVESPDVAAVTGNPRIRNRST
CCCEECCHHHHHHEECEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
LLGRVQVGEFSSIVGIIKRAQRVFGRIFTVSGVITAFRKSAVHQVGYWSADMLTEDIDIT
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEE
WKLQRAGWDVRFEPRALTWILMPETLRGLWKQRLRWAMGGAQVLLKNIDAALEPAQRSLW
EEEECCCCCEEECCCEEEEEECHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHH
PLLVEMVVSVAWSYAMVLVTATWLVGLVLPGGVLPAVGSPMGLQGSGVVLGAACLAQFAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHH
STWLDSRYDRGLFRNLFWMIWYPFVFWVINCAATVVAYPKVVARRRGSRARWVSPDRGFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
P
C
>Mature Secondary Structure 
TATLDSAWQGLLAFVFYYPLFMSFLWMTGAALFYWRFERAERSLDRPPPLPHWPPVTVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
IPSYNEGENAEETVSHALALDYPEFEVVAINDGSSDDTGAVLERLAARDPRLRVVHLAEN
ECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEECC
QGKAMGLQAGALLARHEILVCIDGDALLDPRAAHWLVRHFVESPDVAAVTGNPRIRNRST
CCCEECCHHHHHHEECEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
LLGRVQVGEFSSIVGIIKRAQRVFGRIFTVSGVITAFRKSAVHQVGYWSADMLTEDIDIT
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEE
WKLQRAGWDVRFEPRALTWILMPETLRGLWKQRLRWAMGGAQVLLKNIDAALEPAQRSLW
EEEECCCCCEEECCCEEEEEECHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHH
PLLVEMVVSVAWSYAMVLVTATWLVGLVLPGGVLPAVGSPMGLQGSGVVLGAACLAQFAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHH
STWLDSRYDRGLFRNLFWMIWYPFVFWVINCAATVVAYPKVVARRRGSRARWVSPDRGFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]