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The map label for this gene is lcfB [H]

Identifier: 220918191

GI number: 220918191

Start: 3418847

End: 3420553

Strand: Reverse

Name: lcfB [H]

Synonym: A2cp1_3094

Alternate gene names: 220918191

Gene position: 3420553-3418847 (Counterclockwise)

Preceding gene: 220918193

Following gene: 220918190

Centisome position: 68.01

GC content: 73.11

Gene sequence:

>1707_bases
ATGAAGACCGCCGCCCAGGACCAGGCCTGCCGCGACCGCGCGGCGCGGCTCCCCGAGAACATCGCCGACCGGGTCGCCGA
GCACGCGCGGCGTGCCCCCGGGGCCATCGCGCTGCTCGAGCACAACACCGGCGAGACCGTCACCTGGAAGCAGCTCGACA
CCGCCGCCGACGCGTTCGCGGCGCGGCTGCTCGCCGCGGGCTACCGCAAGGGCGACGTCGTCGCCACCAGCCTGCCGCTG
CTCAAGGAGCACGTCTTCCTGCTCGTGGCCTGCTACCGCGTCGGCGTGATCCTGGCGCCGCTCGACCTGCGGCTGCGCGC
GGGAGAGATCCGGACCGCGTTCGAGAAGCTCCGTCCCAGGGGCTACTTCTTCGTCGGCGCGCCCGCGCTCATGCCGGTGC
TCCAGGAGGTGGTCGGCAAGTTCCCGGGGGTCCGCCACTGGGTGCAGTTCCAGAAGGACCCGGAGGGCATCCTCCCCGGC
GCCACCTGGGCGAAGGACTTCACCCGCCGGATCAAGCTCGACTACCTGAAGGCGAGGCTCCTCGGCACGGTGCGGCGCGC
CCGCGCGCGCGTCGGCAGGCGCGACGGCTGCCTCATCATCTTCACCACCGGCTCGACCGGCTCGCCCAAGCCGGCGCTCC
TCTGCCACGAGAGCATCCTCGTCCAGAACGTCGGGCTCACCGTCGGGTTCGACCTCCGCCCGGACGACCGGCTGCTGGTG
AACCTCCCGCCCTCGCACGTGGGCTGCACCACCGAGCTGCTCGGCACGGCGCTGTACGAGGGCATCACCTCGGTGCTCCT
GCACGTCTTCGACGCGCTGAAGAGTCTCGAGGCGATCGCCGAGCACCGCGTCAGCGTGGTCGGGCAGATCCCCGCGCTCT
TCAACGCAGAGTGGAGCCACCGCCGCTACGCCGAGCTCGACCTCTCCAGCCTGCGCGCCGCCATCTACGGGGGGCAGGGC
GTGCCGCGCGCGTTCCTCGACCGGCTCCGCGCCATGGCCCCGGAGATCGGCACCGGCCTCGGCCTCACCGAGACCTCCGG
CTTCTGCACCTACACCGCCGTGGGCGCGAGCGCCGACGACCTCGCGGACGGCATCGGCTTCGACTCGCCGCTCTGCCCCG
TCTCCATCCGCGGGCCGATGACCGCGGACGGGCACGCCGGCCCCGAGCTGCCCGCGGGGACGGTGGGGGAGATCTGCTTC
TCGGGCCCGCAGGTGTTCCTCGGGTACCTGAACGACCCGGCCGCCACCGCGCAGGCGGTCACGAAGGACGGCGTCTGCTA
CACGGGCGACCTCGGCAGCTACGACCCGGCGCGCGGGCTCCGGCTCGCCGGCCGCGCCAAGCTCGTCATCAAGCCGAAGG
GCTTCCAGGTGTTCCCGGGCGACGTGGAGAACCACGTGGTGTCCGCGCTGCCGGGCAAGGTGGCTGCGGCGGCCTGCGTC
GGCGTCGAGCACGCGCGCTGGTCGGAGGCGATCGTGCTGTTCGTGGAGGAGGCGCCCGACGCCACCGTCACGCCCGAGGA
CGTCCACGCTGCGTGCCAGGGGATCGCCTCGTACGCCCGCCCGAGCCACGTGGAGGTGGTGTCCACCGGGACGCTCCCGC
TCAACCGCGTCGCCAAGGTGGACTACGTCGTGCTGCGCGAGCGCGCCCGCGAGCTGGTCGAGGGTCTCCGGCGCGAGGGG
AAGTGGGACGCGCGGGCCATCCGGTGA

Upstream 100 bases:

>100_bases
CGCCTCGCATGCGCGGCATCTTCGCCCCGGTCCGCGGCGAAGTCGACCTCGCGCCCGCCGCGCTCGCCCGGCCGGGCGGT
TTGCGCTAGAACGCCCCGCC

Downstream 100 bases:

>100_bases
GCGCCACGGCCGCCCGGCGGCCGGCTGGCCACCCCGCCGGGGAACCCCTGCGTCCCTTGTGCGCATCCCCGATTGAAGTA
CCCAGCTCCGGGCGCAATCC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MKTAAQDQACRDRAARLPENIADRVAEHARRAPGAIALLEHNTGETVTWKQLDTAADAFAARLLAAGYRKGDVVATSLPL
LKEHVFLLVACYRVGVILAPLDLRLRAGEIRTAFEKLRPRGYFFVGAPALMPVLQEVVGKFPGVRHWVQFQKDPEGILPG
ATWAKDFTRRIKLDYLKARLLGTVRRARARVGRRDGCLIIFTTGSTGSPKPALLCHESILVQNVGLTVGFDLRPDDRLLV
NLPPSHVGCTTELLGTALYEGITSVLLHVFDALKSLEAIAEHRVSVVGQIPALFNAEWSHRRYAELDLSSLRAAIYGGQG
VPRAFLDRLRAMAPEIGTGLGLTETSGFCTYTAVGASADDLADGIGFDSPLCPVSIRGPMTADGHAGPELPAGTVGEICF
SGPQVFLGYLNDPAATAQAVTKDGVCYTGDLGSYDPARGLRLAGRAKLVIKPKGFQVFPGDVENHVVSALPGKVAAAACV
GVEHARWSEAIVLFVEEAPDATVTPEDVHAACQGIASYARPSHVEVVSTGTLPLNRVAKVDYVVLRERARELVEGLRREG
KWDARAIR

Sequences:

>Translated_568_residues
MKTAAQDQACRDRAARLPENIADRVAEHARRAPGAIALLEHNTGETVTWKQLDTAADAFAARLLAAGYRKGDVVATSLPL
LKEHVFLLVACYRVGVILAPLDLRLRAGEIRTAFEKLRPRGYFFVGAPALMPVLQEVVGKFPGVRHWVQFQKDPEGILPG
ATWAKDFTRRIKLDYLKARLLGTVRRARARVGRRDGCLIIFTTGSTGSPKPALLCHESILVQNVGLTVGFDLRPDDRLLV
NLPPSHVGCTTELLGTALYEGITSVLLHVFDALKSLEAIAEHRVSVVGQIPALFNAEWSHRRYAELDLSSLRAAIYGGQG
VPRAFLDRLRAMAPEIGTGLGLTETSGFCTYTAVGASADDLADGIGFDSPLCPVSIRGPMTADGHAGPELPAGTVGEICF
SGPQVFLGYLNDPAATAQAVTKDGVCYTGDLGSYDPARGLRLAGRAKLVIKPKGFQVFPGDVENHVVSALPGKVAAAACV
GVEHARWSEAIVLFVEEAPDATVTPEDVHAACQGIASYARPSHVEVVSTGTLPLNRVAKVDYVVLRERARELVEGLRREG
KWDARAIR
>Mature_568_residues
MKTAAQDQACRDRAARLPENIADRVAEHARRAPGAIALLEHNTGETVTWKQLDTAADAFAARLLAAGYRKGDVVATSLPL
LKEHVFLLVACYRVGVILAPLDLRLRAGEIRTAFEKLRPRGYFFVGAPALMPVLQEVVGKFPGVRHWVQFQKDPEGILPG
ATWAKDFTRRIKLDYLKARLLGTVRRARARVGRRDGCLIIFTTGSTGSPKPALLCHESILVQNVGLTVGFDLRPDDRLLV
NLPPSHVGCTTELLGTALYEGITSVLLHVFDALKSLEAIAEHRVSVVGQIPALFNAEWSHRRYAELDLSSLRAAIYGGQG
VPRAFLDRLRAMAPEIGTGLGLTETSGFCTYTAVGASADDLADGIGFDSPLCPVSIRGPMTADGHAGPELPAGTVGEICF
SGPQVFLGYLNDPAATAQAVTKDGVCYTGDLGSYDPARGLRLAGRAKLVIKPKGFQVFPGDVENHVVSALPGKVAAAACV
GVEHARWSEAIVLFVEEAPDATVTPEDVHAACQGIASYARPSHVEVVSTGTLPLNRVAKVDYVVLRERARELVEGLRREG
KWDARAIR

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=548, Percent_Identity=25.7299270072993, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI187761345, Length=384, Percent_Identity=24.7395833333333, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI187761343, Length=384, Percent_Identity=24.7395833333333, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI40807491, Length=499, Percent_Identity=25.6513026052104, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI57165412, Length=339, Percent_Identity=25.0737463126844, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI57165410, Length=322, Percent_Identity=24.223602484472, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1788107, Length=569, Percent_Identity=27.9437609841828, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI145693145, Length=544, Percent_Identity=25.1838235294118, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI221142682, Length=520, Percent_Identity=26.1538461538462, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1786810, Length=363, Percent_Identity=28.9256198347107, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71994703, Length=581, Percent_Identity=24.7848537005164, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71994690, Length=579, Percent_Identity=25.2158894645941, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71994694, Length=579, Percent_Identity=25.2158894645941, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=538, Percent_Identity=25.6505576208178, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=496, Percent_Identity=25.2016129032258, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17531443, Length=544, Percent_Identity=23.7132352941176, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17538037, Length=547, Percent_Identity=22.1206581352834, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32563687, Length=370, Percent_Identity=23.7837837837838, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17558820, Length=540, Percent_Identity=21.6666666666667, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71985884, Length=279, Percent_Identity=25.4480286738351, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560140, Length=305, Percent_Identity=25.2459016393443, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24581924, Length=512, Percent_Identity=25.5859375, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI21355181, Length=513, Percent_Identity=25.9259259259259, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI18859661, Length=514, Percent_Identity=24.7081712062257, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648255, Length=516, Percent_Identity=22.4806201550388, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20130357, Length=369, Percent_Identity=24.390243902439, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21356947, Length=563, Percent_Identity=22.7353463587922, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 61104; Mature: 61104

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAAQDQACRDRAARLPENIADRVAEHARRAPGAIALLEHNTGETVTWKQLDTAADAFA
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHHHHH
ARLLAAGYRKGDVVATSLPLLKEHVFLLVACYRVGVILAPLDLRLRAGEIRTAFEKLRPR
HHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCEEEECCCCEEECHHHHHHHHHHCCC
GYFFVGAPALMPVLQEVVGKFPGVRHWVQFQKDPEGILPGATWAKDFTRRIKLDYLKARL
CEEEECCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LGTVRRARARVGRRDGCLIIFTTGSTGSPKPALLCHESILVQNVGLTVGFDLRPDDRLLV
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEHHHHHHEECCEEEEECCCCCCEEEE
NLPPSHVGCTTELLGTALYEGITSVLLHVFDALKSLEAIAEHRVSVVGQIPALFNAEWSH
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCC
RRYAELDLSSLRAAIYGGQGVPRAFLDRLRAMAPEIGTGLGLTETSGFCTYTAVGASADD
CHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEECCCCHHH
LADGIGFDSPLCPVSIRGPMTADGHAGPELPAGTVGEICFSGPQVFLGYLNDPAATAQAV
HHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHCCCHHEEEECCCCHHHHHHH
TKDGVCYTGDLGSYDPARGLRLAGRAKLVIKPKGFQVFPGDVENHVVSALPGKVAAAACV
HCCCCEEECCCCCCCCCCCCEECCCEEEEEECCCCEECCCCHHHHHHHHCCCHHHHHHHH
GVEHARWSEAIVLFVEEAPDATVTPEDVHAACQGIASYARPSHVEVVSTGTLPLNRVAKV
CCHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
DYVVLRERARELVEGLRREGKWDARAIR
HHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKTAAQDQACRDRAARLPENIADRVAEHARRAPGAIALLEHNTGETVTWKQLDTAADAFA
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHHHHH
ARLLAAGYRKGDVVATSLPLLKEHVFLLVACYRVGVILAPLDLRLRAGEIRTAFEKLRPR
HHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCEEEECCCCEEECHHHHHHHHHHCCC
GYFFVGAPALMPVLQEVVGKFPGVRHWVQFQKDPEGILPGATWAKDFTRRIKLDYLKARL
CEEEECCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LGTVRRARARVGRRDGCLIIFTTGSTGSPKPALLCHESILVQNVGLTVGFDLRPDDRLLV
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEHHHHHHEECCEEEEECCCCCCEEEE
NLPPSHVGCTTELLGTALYEGITSVLLHVFDALKSLEAIAEHRVSVVGQIPALFNAEWSH
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCC
RRYAELDLSSLRAAIYGGQGVPRAFLDRLRAMAPEIGTGLGLTETSGFCTYTAVGASADD
CHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEECCCCHHH
LADGIGFDSPLCPVSIRGPMTADGHAGPELPAGTVGEICFSGPQVFLGYLNDPAATAQAV
HHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHCCCHHEEEECCCCHHHHHHH
TKDGVCYTGDLGSYDPARGLRLAGRAKLVIKPKGFQVFPGDVENHVVSALPGKVAAAACV
HCCCCEEECCCCCCCCCCCCEECCCEEEEEECCCCEECCCCHHHHHHHHCCCHHHHHHHH
GVEHARWSEAIVLFVEEAPDATVTPEDVHAACQGIASYARPSHVEVVSTGTLPLNRVAKV
CCHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
DYVVLRERARELVEGLRREGKWDARAIR
HHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]