Click here to switch to the map view.

The map label for this gene is amyB [H]

Identifier: 220918193

GI number: 220918193

Start: 3421410

End: 3422849

Strand: Reverse

Name: amyB [H]

Synonym: A2cp1_3096

Alternate gene names: 220918193

Gene position: 3422849-3421410 (Counterclockwise)

Preceding gene: 220918194

Following gene: 220918191

Centisome position: 68.06

GC content: 76.32

Gene sequence:

>1440_bases
ATGCGCGCCCGCCCGCTCCACCCTCCCGCTCGCGGTTCCGCGGGCTCGACGCGAGCGCCTGCCGGGAGGGCGCGCGCTCC
CCTGGCCGCCCTGCTGGCGCTCCTCTGCGCCGGACCGCTGCGGGCCGGCGCCGACGTCCGGCCGGATCCGGAGTGGTACC
GCGGCGCCGTGGTCTACGGCGTCGTCTCGCCGCGGTTCGGCCCCGAGCCCCTGAAGGCGGTGACCGCGCGGCTCGACGCC
CTCCGCGAGCTCGGCGTAGACGCGCTCTGGCTCGCGCCGCTCAACCCCACCGACGATCCCGGCGACGTCAGCTACGCCAT
CACCGACTACTTCGGCCTGCGCGCCGACTTCGGCACGCCCGAGGACCTCCGCACCCTGGTCCGCGAGGCACACGCCCGCG
GCCTGCGCGTGCTCCTCGACTTCGTCCCGAACCACACCTCGGTCGGCCACCCGCACCACCTCGACGCCGCGGCGCGAGGC
CGGGCCTCGCCGTGGTGGGCCTGGTACGATCGCGATCCGGCCGGCCGAGAGACGCACTACTTCGACTGGAAGCACCTGCC
GAACCTCGACTACGGCAACCCGCTCGTCCGGCAGATGATGCTGGACGCGTTCGCGTACTGGGTCCGCGAGTACGACGTGG
ACGGGTTCCGCGTGGACGCGGCCTGGGGGATCCGCCGGCGCGCGCCGGCGTTCTGGGGGGCGCTGCGCCGGCGGCTCGAC
GCGGTGAAGCCGGGCGTGTTCCTGCTCGCGGAGGCGAGCGCGCGCGACCCGTTCTACGTGCGGAACGGCTTCGACGCGGC
GTACGACTGGACCGGCGAGCTCGGCCACTGGGCCTGGGAGAAGGTCTTTGACGATCCCACGCGCATCGGGCCGGCGCTCG
ACGCCGCGCTCGCCTCCCGCGCGACGCCGATGCACCGGGTGGCGCGCTTCCTCAACAACAACGACACCGGCGAGCGGTTC
GTCACCCGCCACGGCCTCGCCATGACGCGCGTCGCGGCCGTGCTCCTCCACGCGCTGCCCGGCATCGCGGTGGTCTACAC
CGGCGACGAGGTCGGCGCCGAGTTCCAGCCGTACGAGGAGGGGCCGCCGGTGTCCTGGGTGGACGCCCACGGCCTCCGCC
CGCTGTACCGGCGCCTCGCGGCGCTCCGCGAGGACCTACCGGCGCTCCGCCGCGGCGCGTACCGGCGGCTCGCGGTCCCC
GGGCACCCGGAGGCGTTCGCGTTCCTGCGCGACGCGGGGCCGGCGGGGCGCGCGCTGGTGGTGCTCAACTTCGGGGGCCC
GGCGCGGCTCCGCCTGCAGGGCCCGGCGGGCCCGCTGCCGGACTGGGACGCGATCTCGCAGCGACCGGTGGCGGTGCGGC
GCGCGGGCGCCGGCACCCTGGAGGTCGAGCTGGGCGCGCAGGAGGCCGTGATCCTGGTGGACCGCGCCGGCCGGCGCTGA

Upstream 100 bases:

>100_bases
AACCGGGCGAGGCGAGCGCATCCTGCTCGCCTCGCCCTTCTCGTCTCCGGGCTCACGCGCTCCCTGGAACACCGCCGCGC
CGCGCGGCGTTGCTCAGGGG

Downstream 100 bases:

>100_bases
CCGCGGCCTACGCCTGCTCCGACCCCGGGCCGCTCCCCTCGGGCGCCGTGCCGCGCGCGCGGCGCCGGTCGAGGAGGTCC
GCCGCCAGCCAGCAGGCGAC

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MRARPLHPPARGSAGSTRAPAGRARAPLAALLALLCAGPLRAGADVRPDPEWYRGAVVYGVVSPRFGPEPLKAVTARLDA
LRELGVDALWLAPLNPTDDPGDVSYAITDYFGLRADFGTPEDLRTLVREAHARGLRVLLDFVPNHTSVGHPHHLDAAARG
RASPWWAWYDRDPAGRETHYFDWKHLPNLDYGNPLVRQMMLDAFAYWVREYDVDGFRVDAAWGIRRRAPAFWGALRRRLD
AVKPGVFLLAEASARDPFYVRNGFDAAYDWTGELGHWAWEKVFDDPTRIGPALDAALASRATPMHRVARFLNNNDTGERF
VTRHGLAMTRVAAVLLHALPGIAVVYTGDEVGAEFQPYEEGPPVSWVDAHGLRPLYRRLAALREDLPALRRGAYRRLAVP
GHPEAFAFLRDAGPAGRALVVLNFGGPARLRLQGPAGPLPDWDAISQRPVAVRRAGAGTLEVELGAQEAVILVDRAGRR

Sequences:

>Translated_479_residues
MRARPLHPPARGSAGSTRAPAGRARAPLAALLALLCAGPLRAGADVRPDPEWYRGAVVYGVVSPRFGPEPLKAVTARLDA
LRELGVDALWLAPLNPTDDPGDVSYAITDYFGLRADFGTPEDLRTLVREAHARGLRVLLDFVPNHTSVGHPHHLDAAARG
RASPWWAWYDRDPAGRETHYFDWKHLPNLDYGNPLVRQMMLDAFAYWVREYDVDGFRVDAAWGIRRRAPAFWGALRRRLD
AVKPGVFLLAEASARDPFYVRNGFDAAYDWTGELGHWAWEKVFDDPTRIGPALDAALASRATPMHRVARFLNNNDTGERF
VTRHGLAMTRVAAVLLHALPGIAVVYTGDEVGAEFQPYEEGPPVSWVDAHGLRPLYRRLAALREDLPALRRGAYRRLAVP
GHPEAFAFLRDAGPAGRALVVLNFGGPARLRLQGPAGPLPDWDAISQRPVAVRRAGAGTLEVELGAQEAVILVDRAGRR
>Mature_479_residues
MRARPLHPPARGSAGSTRAPAGRARAPLAALLALLCAGPLRAGADVRPDPEWYRGAVVYGVVSPRFGPEPLKAVTARLDA
LRELGVDALWLAPLNPTDDPGDVSYAITDYFGLRADFGTPEDLRTLVREAHARGLRVLLDFVPNHTSVGHPHHLDAAARG
RASPWWAWYDRDPAGRETHYFDWKHLPNLDYGNPLVRQMMLDAFAYWVREYDVDGFRVDAAWGIRRRAPAFWGALRRRLD
AVKPGVFLLAEASARDPFYVRNGFDAAYDWTGELGHWAWEKVFDDPTRIGPALDAALASRATPMHRVARFLNNNDTGERF
VTRHGLAMTRVAAVLLHALPGIAVVYTGDEVGAEFQPYEEGPPVSWVDAHGLRPLYRRLAALREDLPALRRGAYRRLAVP
GHPEAFAFLRDAGPAGRALVVLNFGGPARLRLQGPAGPLPDWDAISQRPVAVRRAGAGTLEVELGAQEAVILVDRAGRR

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=203, Percent_Identity=25.1231527093596, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1790687, Length=378, Percent_Identity=26.1904761904762, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786604, Length=404, Percent_Identity=27.7227722772277, Blast_Score=82, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6322245, Length=212, Percent_Identity=29.7169811320755, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322241, Length=218, Percent_Identity=26.605504587156, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322021, Length=218, Percent_Identity=26.605504587156, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324416, Length=218, Percent_Identity=26.605504587156, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6321726, Length=218, Percent_Identity=26.605504587156, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6321731, Length=219, Percent_Identity=26.027397260274, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319776, Length=219, Percent_Identity=26.027397260274, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320826, Length=163, Percent_Identity=30.0613496932515, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24586599, Length=521, Percent_Identity=23.2245681381958, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24583747, Length=238, Percent_Identity=33.1932773109244, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24586587, Length=209, Percent_Identity=31.1004784688995, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24583745, Length=411, Percent_Identity=25.5474452554745, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24586591, Length=213, Percent_Identity=31.924882629108, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24586597, Length=209, Percent_Identity=30.1435406698565, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24583749, Length=213, Percent_Identity=31.4553990610329, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI221330053, Length=247, Percent_Identity=28.7449392712551, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24586593, Length=257, Percent_Identity=28.7937743190661, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45549022, Length=233, Percent_Identity=26.1802575107296, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 52750; Mature: 52750

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRARPLHPPARGSAGSTRAPAGRARAPLAALLALLCAGPLRAGADVRPDPEWYRGAVVYG
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEE
VVSPRFGPEPLKAVTARLDALRELGVDALWLAPLNPTDDPGDVSYAITDYFGLRADFGTP
EECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEHHHCCCCCCCCC
EDLRTLVREAHARGLRVLLDFVPNHTSVGHPHHLDAAARGRASPWWAWYDRDPAGRETHY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCCCCCE
FDWKHLPNLDYGNPLVRQMMLDAFAYWVREYDVDGFRVDAAWGIRRRAPAFWGALRRRLD
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCHHCCHHHHHHHHHHHH
AVKPGVFLLAEASARDPFYVRNGFDAAYDWTGELGHWAWEKVFDDPTRIGPALDAALASR
HCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCHHHHCHHHHHHHHHC
ATPMHRVARFLNNNDTGERFVTRHGLAMTRVAAVLLHALPGIAVVYTGDEVGAEFQPYEE
CCHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECHHCCCCCCCCCC
GPPVSWVDAHGLRPLYRRLAALREDLPALRRGAYRRLAVPGHPEAFAFLRDAGPAGRALV
CCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHCCCCCCCEEE
VLNFGGPARLRLQGPAGPLPDWDAISQRPVAVRRAGAGTLEVELGAQEAVILVDRAGRR
EEECCCCEEEEECCCCCCCCCCHHHCCCCHHEEECCCCEEEEEECCCEEEEEEECCCCC
>Mature Secondary Structure
MRARPLHPPARGSAGSTRAPAGRARAPLAALLALLCAGPLRAGADVRPDPEWYRGAVVYG
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEE
VVSPRFGPEPLKAVTARLDALRELGVDALWLAPLNPTDDPGDVSYAITDYFGLRADFGTP
EECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEHHHCCCCCCCCC
EDLRTLVREAHARGLRVLLDFVPNHTSVGHPHHLDAAARGRASPWWAWYDRDPAGRETHY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCCCCCE
FDWKHLPNLDYGNPLVRQMMLDAFAYWVREYDVDGFRVDAAWGIRRRAPAFWGALRRRLD
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCHHCCHHHHHHHHHHHH
AVKPGVFLLAEASARDPFYVRNGFDAAYDWTGELGHWAWEKVFDDPTRIGPALDAALASR
HCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCHHHHCHHHHHHHHHC
ATPMHRVARFLNNNDTGERFVTRHGLAMTRVAAVLLHALPGIAVVYTGDEVGAEFQPYEE
CCHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECHHCCCCCCCCCC
GPPVSWVDAHGLRPLYRRLAALREDLPALRRGAYRRLAVPGHPEAFAFLRDAGPAGRALV
CCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHCCCCCCCEEE
VLNFGGPARLRLQGPAGPLPDWDAISQRPVAVRRAGAGTLEVELGAQEAVILVDRAGRR
EEECCCCEEEEECCCCCCCCCCHHHCCCCHHEEECCCCEEEEEECCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2458257 [H]