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The map label for this gene is thiC

Identifier: 220917404

GI number: 220917404

Start: 2553699

End: 2555090

Strand: Reverse

Name: thiC

Synonym: A2cp1_2304

Alternate gene names: 220917404

Gene position: 2555090-2553699 (Counterclockwise)

Preceding gene: 220917405

Following gene: 220917402

Centisome position: 50.8

GC content: 70.91

Gene sequence:

>1392_bases
ATGCGCGCCGACTGGATCGCGAAGCGTCGTGGGCAGGCCAACGTCAGCCAGATGCACTACGCCCGCAGCGGCGTCGTCAC
CGAGGAGATGGCGTTCGTGGCGGCGCGGGAGCGCCTCGAGCCCGAGCTGGTCCGCGCCGAGGTGGCCCGGGGCCGGATGG
TGATCCCGGCGAACGTCCGCCACCCCGAGCTCGAGCCGCTCGCCATCGGCATCACGGCCTGCTGCAAGATCAACGCGAAC
ATCGGCAACAGCGCGGTCACCTCGAACATCGACGAGGAGCTGGCCAAGCTGCGGGTGTGCCTGAAGTACGGCGCCGACAC
GGTGATGGACCTCTCCACCGGCGGCGACATCCCGCAGATCCGCGAGGCGATCCTGCGCGCCAGCCCGATCCCGGTGGGCA
CGGTGCCGATCTACGAGTGCCTGGCGCACGTGAAGGACGTGGCCGACCTCACGCCCGAGCTGATGCTCGAGATCATCGAG
GCGCAGGCGGCGCAGGGCGTGGACTACATGACCATCCACGCCGGCGTGCTGCGGGACTTCATCCCGCTCGCGGCGCACCG
GATCACCGGCATCGTCTCGCGCGGCGGCGCCCTGATGGCGCAGTGGATGCTCGCGAACAAGGCCGAGAACCCGTTCTTCA
CGCACTTCGAGCAGATCTGCGAGATCTTCAAGCGCTACGACGTCACCTTCTCGCTCGGCGACGGCCTGCGCCCCGGGTGC
CTCGCCGACGCGTCCGATGCGGCGCAGTTCGCGGAGCTGAAGGCGCTCGGCGACCTGACGCGCAAGGCGTGGGAGCACGG
CGTGCAGGTGATGGTCGAGGGCCCGGGCCACGTCCCGCTCGACCAGATCCCCATGAACATGGAGAAGGAGCGGGAGCTCT
GCAGCGAGGCCCCGTTCTACGTGCTCGGGCCGCTCGTCACCGACATCGCCCCCGGCTACGACCACATCACGAGCGCCATC
GGCGCGGCGGTGGCGGCGCAGCACGGCGCGGCCATGCTCTGCTACGTCACGCCCGCCGAGCACCTCGGGCTGCCGGACAT
CGACGACGTCCGCGAGGGCATCGTCGCCTACAAGATCGCGGCGCACGCGGCCGACGTGGCGCGCCACCGGCCGGGCGCCC
GCGACCGCGACGACGCGCTCTCCCGCGCCCGCTACGCGTTCGACTGGAACAAGCAGTTCGAGCTGTCGCTCGACCCGGAC
ACCGCGCGCGCGAAGCACGACGAGACGCTGCCGCACGAGGCGTTCAAGTCGGCGGAGTTCTGCTCGATGTGCGGGCCGAA
GTTCTGCTCGATGAAGATCCACGGCCACCTCGCCGAGGCGGCCGCCGCGCAGGACGCGGCGGCGAACGGCGAGGCCGAGA
AGCCGGCCGGCGGCCTCCAGGTCCTCCCGTAG

Upstream 100 bases:

>100_bases
CCCGCCCACGCGGATTTCCGGGCGCGGCGCATTGACACGCCCGCGGCCCCTCGCTAGCTTCACGTCCCTTGCATCGACCC
TCCCGTCACAGGTGAAGAGC

Downstream 100 bases:

>100_bases
GCGCACCCGGAGGCCTCGGCCTTCACCGCCCCGGCGGTCCCTCCTCCACCGGCCTGCCGGCGGCCACCCAGCCGTCGATG
CCGGCCGGCATCACGTACAC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MRADWIAKRRGQANVSQMHYARSGVVTEEMAFVAARERLEPELVRAEVARGRMVIPANVRHPELEPLAIGITACCKINAN
IGNSAVTSNIDEELAKLRVCLKYGADTVMDLSTGGDIPQIREAILRASPIPVGTVPIYECLAHVKDVADLTPELMLEIIE
AQAAQGVDYMTIHAGVLRDFIPLAAHRITGIVSRGGALMAQWMLANKAENPFFTHFEQICEIFKRYDVTFSLGDGLRPGC
LADASDAAQFAELKALGDLTRKAWEHGVQVMVEGPGHVPLDQIPMNMEKERELCSEAPFYVLGPLVTDIAPGYDHITSAI
GAAVAAQHGAAMLCYVTPAEHLGLPDIDDVREGIVAYKIAAHAADVARHRPGARDRDDALSRARYAFDWNKQFELSLDPD
TARAKHDETLPHEAFKSAEFCSMCGPKFCSMKIHGHLAEAAAAQDAAANGEAEKPAGGLQVLP

Sequences:

>Translated_463_residues
MRADWIAKRRGQANVSQMHYARSGVVTEEMAFVAARERLEPELVRAEVARGRMVIPANVRHPELEPLAIGITACCKINAN
IGNSAVTSNIDEELAKLRVCLKYGADTVMDLSTGGDIPQIREAILRASPIPVGTVPIYECLAHVKDVADLTPELMLEIIE
AQAAQGVDYMTIHAGVLRDFIPLAAHRITGIVSRGGALMAQWMLANKAENPFFTHFEQICEIFKRYDVTFSLGDGLRPGC
LADASDAAQFAELKALGDLTRKAWEHGVQVMVEGPGHVPLDQIPMNMEKERELCSEAPFYVLGPLVTDIAPGYDHITSAI
GAAVAAQHGAAMLCYVTPAEHLGLPDIDDVREGIVAYKIAAHAADVARHRPGARDRDDALSRARYAFDWNKQFELSLDPD
TARAKHDETLPHEAFKSAEFCSMCGPKFCSMKIHGHLAEAAAAQDAAANGEAEKPAGGLQVLP
>Mature_463_residues
MRADWIAKRRGQANVSQMHYARSGVVTEEMAFVAARERLEPELVRAEVARGRMVIPANVRHPELEPLAIGITACCKINAN
IGNSAVTSNIDEELAKLRVCLKYGADTVMDLSTGGDIPQIREAILRASPIPVGTVPIYECLAHVKDVADLTPELMLEIIE
AQAAQGVDYMTIHAGVLRDFIPLAAHRITGIVSRGGALMAQWMLANKAENPFFTHFEQICEIFKRYDVTFSLGDGLRPGC
LADASDAAQFAELKALGDLTRKAWEHGVQVMVEGPGHVPLDQIPMNMEKERELCSEAPFYVLGPLVTDIAPGYDHITSAI
GAAVAAQHGAAMLCYVTPAEHLGLPDIDDVREGIVAYKIAAHAADVARHRPGARDRDDALSRARYAFDWNKQFELSLDPD
TARAKHDETLPHEAFKSAEFCSMCGPKFCSMKIHGHLAEAAAAQDAAANGEAEKPAGGLQVLP

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=458, Percent_Identity=57.6419213973799, Blast_Score=539, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_ANAD2 (B8JAC3)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002492708.1
- GeneID:   7299270
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_2304
- HOGENOM:   HBG285234
- ProtClustDB:   PRK13352
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 50213; Mature: 50213

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: BINDING 80-80 BINDING 109-109 BINDING 138-138 BINDING 173-173 BINDING 273-273 BINDING 300-300

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRADWIAKRRGQANVSQMHYARSGVVTEEMAFVAARERLEPELVRAEVARGRMVIPANVR
CCCCHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCC
HPELEPLAIGITACCKINANIGNSAVTSNIDEELAKLRVCLKYGADTVMDLSTGGDIPQI
CCCCCHHEEEEEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
REAILRASPIPVGTVPIYECLAHVKDVADLTPELMLEIIEAQAAQGVDYMTIHAGVLRDF
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHH
IPLAAHRITGIVSRGGALMAQWMLANKAENPFFTHFEQICEIFKRYDVTFSLGDGLRPGC
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCC
LADASDAAQFAELKALGDLTRKAWEHGVQVMVEGPGHVPLDQIPMNMEKERELCSEAPFY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHCCCCHHHHHHHHHHCCCE
VLGPLVTDIAPGYDHITSAIGAAVAAQHGAAMLCYVTPAEHLGLPDIDDVREGIVAYKIA
EEHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCHHHCCCCCHHHHHHHHHHHHHH
AHAADVARHRPGARDRDDALSRARYAFDWNKQFELSLDPDTARAKHDETLPHEAFKSAEF
HHHHHHHHCCCCCCCHHHHHHHHHHEECCCCEEEEEECCCHHHCCCCCCCCHHHHHCHHH
CSMCGPKFCSMKIHGHLAEAAAAQDAAANGEAEKPAGGLQVLP
HHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRADWIAKRRGQANVSQMHYARSGVVTEEMAFVAARERLEPELVRAEVARGRMVIPANVR
CCCCHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCC
HPELEPLAIGITACCKINANIGNSAVTSNIDEELAKLRVCLKYGADTVMDLSTGGDIPQI
CCCCCHHEEEEEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
REAILRASPIPVGTVPIYECLAHVKDVADLTPELMLEIIEAQAAQGVDYMTIHAGVLRDF
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHH
IPLAAHRITGIVSRGGALMAQWMLANKAENPFFTHFEQICEIFKRYDVTFSLGDGLRPGC
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCC
LADASDAAQFAELKALGDLTRKAWEHGVQVMVEGPGHVPLDQIPMNMEKERELCSEAPFY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHCCCCHHHHHHHHHHCCCE
VLGPLVTDIAPGYDHITSAIGAAVAAQHGAAMLCYVTPAEHLGLPDIDDVREGIVAYKIA
EEHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCHHHCCCCCHHHHHHHHHHHHHH
AHAADVARHRPGARDRDDALSRARYAFDWNKQFELSLDPDTARAKHDETLPHEAFKSAEF
HHHHHHHHCCCCCCCHHHHHHHHHHEECCCCEEEEEECCCHHHCCCCCCCCHHHHHCHHH
CSMCGPKFCSMKIHGHLAEAAAAQDAAANGEAEKPAGGLQVLP
HHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA