Click here to switch to the map view.

The map label for this gene is yngG [H]

Identifier: 220917402

GI number: 220917402

Start: 2552201

End: 2553136

Strand: Reverse

Name: yngG [H]

Synonym: A2cp1_2302

Alternate gene names: 220917402

Gene position: 2553136-2552201 (Counterclockwise)

Preceding gene: 220917404

Following gene: 220917401

Centisome position: 50.76

GC content: 75.75

Gene sequence:

>936_bases
ATGGCCCACCGGGTGACGGTGTACGAGGTCGGCCCGCGGGACGGGCTCCAGAACGAGGCGCGCACCGTCCCGACCGCGGA
CAAGCTGGCGCTCATCGGGGCGCTCGCGGCCGCGGGCCTGCGGCGCATCGAGGCCTCGAGCTTCGTGTCGCCGCGCTGGA
TCCCGCAGCTCGCCGACGCCGCCGAGGTGACCGCGGCGCTCCCGGCGGTGGACGGGACCTCGTACGTGGTGCTGGTGCCG
AACGCGAAGGGGCTGGACCGGCTCGCCGTCGCGCTGGCGCGGGCGGGAGAGGGACATCCGCCGGTGGAGGCCGCGGTGTT
CCTCTCGGCCTCCGAGACGCACAACCGCAAGAACCTGAACCGAACGGTGGCGGAGACGCTGGGCGAGCTGGAGGCGCTCG
TGCCCGCGGCGCTCGCCCGCGGGCTCCGCGTGCGCGGGTACGTCTCCACGGTGTGGGGCTGTCCGTACGAGGGCCCGGTC
GACCCGGGCCGCGCCGCCGAGATCGTCGAGCGGCTGCTCGCGCTCGGCTGCCACCAGGTGTCGCTCGGCGACACCATCGG
CGTGGGGACGCCCAACCAGACCCGGCGCATCCTCGGGCTGCTGCGCGCGCGCGCCCGGCCGGAGCAGATCGCGCTGCACA
TGCACGACACCCGCGGCACCGCGCTCGCGAACGTGCTGGCCGGCCTGGAGGAGGGCGTCACGACGTTCGACGCGTCGATC
GGCGGCCTGGGCGGCTGCCCGTACGCGCCGGGCGCCTCCGGGAACCTCGCCACCGAGGACCTCGTCTACATGCTCGACGG
GATGGGGCTCGAGACCGGAGTGGACTTCCGGAAGCTGGTGGCCGCCGGCGCGCTGGCGCAGCGGCTGGTGGGGCGGACCT
TGCCGGGCAAGGCGCTCCAGGCGGAGCTGGCGCGGGCGGCGCCGGCAGGAGGCTGA

Upstream 100 bases:

>100_bases
GGGAGGGGAGTTAACCCGGGGCGCCGGCGCCGTCATCGCCCCGCGCGGACCCACGCGGGATCACCGGTCCGGCCCGGACC
TGGGCCTTAATATCCGCCAC

Downstream 100 bases:

>100_bases
CATGGATCTCCGGGTGGAGCGCAGAGACGCGGTCGAGGTCTGGACGATCGACGGCGCCTCGCGCCGGAACTCGATCACCG
TGGGGCTGCTCGGCGCGCTG

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MAHRVTVYEVGPRDGLQNEARTVPTADKLALIGALAAAGLRRIEASSFVSPRWIPQLADAAEVTAALPAVDGTSYVVLVP
NAKGLDRLAVALARAGEGHPPVEAAVFLSASETHNRKNLNRTVAETLGELEALVPAALARGLRVRGYVSTVWGCPYEGPV
DPGRAAEIVERLLALGCHQVSLGDTIGVGTPNQTRRILGLLRARARPEQIALHMHDTRGTALANVLAGLEEGVTTFDASI
GGLGGCPYAPGASGNLATEDLVYMLDGMGLETGVDFRKLVAAGALAQRLVGRTLPGKALQAELARAAPAGG

Sequences:

>Translated_311_residues
MAHRVTVYEVGPRDGLQNEARTVPTADKLALIGALAAAGLRRIEASSFVSPRWIPQLADAAEVTAALPAVDGTSYVVLVP
NAKGLDRLAVALARAGEGHPPVEAAVFLSASETHNRKNLNRTVAETLGELEALVPAALARGLRVRGYVSTVWGCPYEGPV
DPGRAAEIVERLLALGCHQVSLGDTIGVGTPNQTRRILGLLRARARPEQIALHMHDTRGTALANVLAGLEEGVTTFDASI
GGLGGCPYAPGASGNLATEDLVYMLDGMGLETGVDFRKLVAAGALAQRLVGRTLPGKALQAELARAAPAGG
>Mature_310_residues
AHRVTVYEVGPRDGLQNEARTVPTADKLALIGALAAAGLRRIEASSFVSPRWIPQLADAAEVTAALPAVDGTSYVVLVPN
AKGLDRLAVALARAGEGHPPVEAAVFLSASETHNRKNLNRTVAETLGELEALVPAALARGLRVRGYVSTVWGCPYEGPVD
PGRAAEIVERLLALGCHQVSLGDTIGVGTPNQTRRILGLLRARARPEQIALHMHDTRGTALANVLAGLEEGVTTFDASIG
GLGGCPYAPGASGNLATEDLVYMLDGMGLETGVDFRKLVAAGALAQRLVGRTLPGKALQAELARAAPAGG

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=299, Percent_Identity=46.1538461538462, Blast_Score=285, Evalue=4e-77,
Organism=Homo sapiens, GI109150427, Length=299, Percent_Identity=45.819397993311, Blast_Score=282, Evalue=2e-76,
Organism=Homo sapiens, GI109150422, Length=299, Percent_Identity=45.819397993311, Blast_Score=282, Evalue=2e-76,
Organism=Homo sapiens, GI260654708, Length=147, Percent_Identity=47.6190476190476, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17510563, Length=279, Percent_Identity=49.4623655913978, Blast_Score=268, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24582381, Length=300, Percent_Identity=49.6666666666667, Blast_Score=286, Evalue=1e-77,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 32231; Mature: 32100

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00104 EPSP_SYNTHASE_1 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHRVTVYEVGPRDGLQNEARTVPTADKLALIGALAAAGLRRIEASSFVSPRWIPQLADA
CCCEEEEEEECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AEVTAALPAVDGTSYVVLVPNAKGLDRLAVALARAGEGHPPVEAAVFLSASETHNRKNLN
HHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEEECHHHCCHHHHH
RTVAETLGELEALVPAALARGLRVRGYVSTVWGCPYEGPVDPGRAAEIVERLLALGCHQV
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEE
SLGDTIGVGTPNQTRRILGLLRARARPEQIALHMHDTRGTALANVLAGLEEGVTTFDASI
CCCCEECCCCCHHHHHHHHHHHHCCCHHHEEEEEECCCHHHHHHHHHHHHHCCHHHHHCC
GGLGGCPYAPGASGNLATEDLVYMLDGMGLETGVDFRKLVAAGALAQRLVGRTLPGKALQ
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
AELARAAPAGG
HHHHHCCCCCC
>Mature Secondary Structure 
AHRVTVYEVGPRDGLQNEARTVPTADKLALIGALAAAGLRRIEASSFVSPRWIPQLADA
CCEEEEEEECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AEVTAALPAVDGTSYVVLVPNAKGLDRLAVALARAGEGHPPVEAAVFLSASETHNRKNLN
HHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEEECHHHCCHHHHH
RTVAETLGELEALVPAALARGLRVRGYVSTVWGCPYEGPVDPGRAAEIVERLLALGCHQV
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEE
SLGDTIGVGTPNQTRRILGLLRARARPEQIALHMHDTRGTALANVLAGLEEGVTTFDASI
CCCCEECCCCCHHHHHHHHHHHHCCCHHHEEEEEECCCHHHHHHHHHHHHHCCHHHHHCC
GGLGGCPYAPGASGNLATEDLVYMLDGMGLETGVDFRKLVAAGALAQRLVGRTLPGKALQ
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
AELARAAPAGG
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]