Click here to switch to the map view.

The map label for this gene is aspA [H]

Identifier: 220917278

GI number: 220917278

Start: 2419225

End: 2420652

Strand: Direct

Name: aspA [H]

Synonym: A2cp1_2177

Alternate gene names: 220917278

Gene position: 2419225-2420652 (Clockwise)

Preceding gene: 220917277

Following gene: 220917279

Centisome position: 48.1

GC content: 69.4

Gene sequence:

>1428_bases
ATGACGACGAAGACGAGGCTGGAGCACGACTTCCTGGGCGAGCGCGAGCTCCCGGACGACCGCTACTACGGCGTCCAGAC
GCTGCGCGCGCTCGAGAACTTCCCGATCACCGGGGTCGCCATCTCCAGCGTCCCGGAGCTGGTGCGCGCGCTGGCGCAGG
TGAAGAAGGCGGCCGCGCTCGCGAACGGCGAGCTGGGCGTCATCCCGGCCGACGTGTCGCGCGCCATCTGCGCGGCCTGC
GACGAGATCGTCGCGGGCGCGCTGCACGACCAGTTCCCGGTGGACGTCATCCAGGGCGGCGCCGGCACCTCCACCAACAT
GAACGCGAACGAGGTCATCGCGAACCGCGCGCTCGAGATCCTGGGCAAGCGCAAGGGCGAGTACCAGTTCGTGCACCCGA
ACAACCACGTCAACTGCTCGCAGTCCACCAACGACGCCTACCCGACGGCGTTCCGCATCGCGCTCCACGCGAAGCTCGGC
CAGCTCATCGCCGAGCTGCGCCGGCTGCAGCAGGCGTTCGCCGCGAAGGGCCGCGAGTTCGCCGACGTGCTGAAGATGGG
GCGCACCCAGCTCCAGGACGCGGTCCCGATGTCGCTCGGCCAGGAGTTCGACGCGTACGCGACCACGCTCGGCGAGGACG
TTCAGCGGCTGGAGGAGGCGCGCGCCCTGGTGCTCGAGGTCAACATGGGCGCCACCGCCATCGGCACCGGCATCAACGCG
CCGCCCCGCTACACCGAGCTCGTCACCCGCCGCCTGCGCGAGATCGTGGGCACGCCGCTCGTCTCGTCGGAGAACCTCAT
CGAGGCGACCTGGGACACCGGCGCGTACGTGCAGATCGCGGGCGTGCTGAAGCGCGTCGCGGTGAAGACCTCCAAGATCT
GCAGCGACCTCCGCCTGCTCTCCTCCGGCCCGCGCACCGGCCTGGGCGAGATCAACCTGCCGCCGCTCCAGCCCGGCTCG
TCGATCATGCCGGGCAAGGTGAACCCGGTGATGCCGGAGGTGGTGAACCAGATCTGCTACTACGCCATCGGCGCCGACCT
GACGGTCACGCTGGCGGCCGAGAACGGGCAGCTCGAGCTGAACGTGATGGAGCCGGTGATCGGCTACGGCCTGTTCACGT
CCATCTCGTTCATGGTGAACGGGTTCCGCGTCCTGCGCGAGAAGTGCGTGGACGGGATCACCGCGAACCGCGAGCGCTGC
CGCGAGCTGGTGGTGAACAGCATCGGCATCGTCACCGCGCTCAACCCCATCATCGGCTACGAGGCCTCGGCGGCGGTCGC
CCGCGAGGCGCTGGTCACGGGCCGCTCGGTGGCCGAGCTGGTGCTGGAGAAGCAGCTGGTCTCGAAGGAGCTGCTCGACG
AGGTGCTCTCCACCGAGAACCTGCTCCGCCCCCGCTACATCAACCCGCGCCTGCAGAAGGTCGCGTAG

Upstream 100 bases:

>100_bases
ACCTGGCGACGGCGCTCGCGTTCCGGCGCGCGCGCCCGCCTGGCACCGCGGTTGCCAATGGCCTCCTCCGTGAGCCGCCC
GGAGGCGGCGAGGAGACCGC

Downstream 100 bases:

>100_bases
GCGGCGCCGCCATGATCTGGCTCCAGCTCGTGGTGGTCCTCGCCGCCATCTTCATCGGCGCCCGGCTCGGCGGCATCGGC
CTGGGCGTGATGGGCGGCCT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTTKTRLEHDFLGERELPDDRYYGVQTLRALENFPITGVAISSVPELVRALAQVKKAAALANGELGVIPADVSRAICAAC
DEIVAGALHDQFPVDVIQGGAGTSTNMNANEVIANRALEILGKRKGEYQFVHPNNHVNCSQSTNDAYPTAFRIALHAKLG
QLIAELRRLQQAFAAKGREFADVLKMGRTQLQDAVPMSLGQEFDAYATTLGEDVQRLEEARALVLEVNMGATAIGTGINA
PPRYTELVTRRLREIVGTPLVSSENLIEATWDTGAYVQIAGVLKRVAVKTSKICSDLRLLSSGPRTGLGEINLPPLQPGS
SIMPGKVNPVMPEVVNQICYYAIGADLTVTLAAENGQLELNVMEPVIGYGLFTSISFMVNGFRVLREKCVDGITANRERC
RELVVNSIGIVTALNPIIGYEASAAVAREALVTGRSVAELVLEKQLVSKELLDEVLSTENLLRPRYINPRLQKVA

Sequences:

>Translated_475_residues
MTTKTRLEHDFLGERELPDDRYYGVQTLRALENFPITGVAISSVPELVRALAQVKKAAALANGELGVIPADVSRAICAAC
DEIVAGALHDQFPVDVIQGGAGTSTNMNANEVIANRALEILGKRKGEYQFVHPNNHVNCSQSTNDAYPTAFRIALHAKLG
QLIAELRRLQQAFAAKGREFADVLKMGRTQLQDAVPMSLGQEFDAYATTLGEDVQRLEEARALVLEVNMGATAIGTGINA
PPRYTELVTRRLREIVGTPLVSSENLIEATWDTGAYVQIAGVLKRVAVKTSKICSDLRLLSSGPRTGLGEINLPPLQPGS
SIMPGKVNPVMPEVVNQICYYAIGADLTVTLAAENGQLELNVMEPVIGYGLFTSISFMVNGFRVLREKCVDGITANRERC
RELVVNSIGIVTALNPIIGYEASAAVAREALVTGRSVAELVLEKQLVSKELLDEVLSTENLLRPRYINPRLQKVA
>Mature_474_residues
TTKTRLEHDFLGERELPDDRYYGVQTLRALENFPITGVAISSVPELVRALAQVKKAAALANGELGVIPADVSRAICAACD
EIVAGALHDQFPVDVIQGGAGTSTNMNANEVIANRALEILGKRKGEYQFVHPNNHVNCSQSTNDAYPTAFRIALHAKLGQ
LIAELRRLQQAFAAKGREFADVLKMGRTQLQDAVPMSLGQEFDAYATTLGEDVQRLEEARALVLEVNMGATAIGTGINAP
PRYTELVTRRLREIVGTPLVSSENLIEATWDTGAYVQIAGVLKRVAVKTSKICSDLRLLSSGPRTGLGEINLPPLQPGSS
IMPGKVNPVMPEVVNQICYYAIGADLTVTLAAENGQLELNVMEPVIGYGLFTSISFMVNGFRVLREKCVDGITANRERCR
ELVVNSIGIVTALNPIIGYEASAAVAREALVTGRSVAELVLEKQLVSKELLDEVLSTENLLRPRYINPRLQKVA

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=40.997830802603, Blast_Score=358, Evalue=4e-99,
Organism=Escherichia coli, GI87082375, Length=471, Percent_Identity=58.3864118895966, Blast_Score=543, Evalue=1e-156,
Organism=Escherichia coli, GI1787896, Length=458, Percent_Identity=40.3930131004367, Blast_Score=349, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17553882, Length=463, Percent_Identity=41.2526997840173, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=38.5321100917431, Blast_Score=249, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6324993, Length=466, Percent_Identity=41.8454935622318, Blast_Score=373, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640179, Length=462, Percent_Identity=41.3419913419913, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640177, Length=462, Percent_Identity=41.3419913419913, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24662684, Length=464, Percent_Identity=41.8103448275862, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=40.3887688984881, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24583245, Length=464, Percent_Identity=38.7931034482759, Blast_Score=335, Evalue=5e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51457; Mature: 51326

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKTRLEHDFLGERELPDDRYYGVQTLRALENFPITGVAISSVPELVRALAQVKKAAAL
CCCCHHCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHH
ANGELGVIPADVSRAICAACDEIVAGALHDQFPVDVIQGGAGTSTNMNANEVIANRALEI
CCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHH
LGKRKGEYQFVHPNNHVNCSQSTNDAYPTAFRIALHAKLGQLIAELRRLQQAFAAKGREF
HHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ADVLKMGRTQLQDAVPMSLGQEFDAYATTLGEDVQRLEEARALVLEVNMGATAIGTGINA
HHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEECCCCCC
PPRYTELVTRRLREIVGTPLVSSENLIEATWDTGAYVQIAGVLKRVAVKTSKICSDLRLL
CCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SSGPRTGLGEINLPPLQPGSSIMPGKVNPVMPEVVNQICYYAIGADLTVTLAAENGQLEL
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCEEEE
NVMEPVIGYGLFTSISFMVNGFRVLREKCVDGITANRERCRELVVNSIGIVTALNPIIGY
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
EASAAVAREALVTGRSVAELVLEKQLVSKELLDEVLSTENLLRPRYINPRLQKVA
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCC
>Mature Secondary Structure 
TTKTRLEHDFLGERELPDDRYYGVQTLRALENFPITGVAISSVPELVRALAQVKKAAAL
CCCHHCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHH
ANGELGVIPADVSRAICAACDEIVAGALHDQFPVDVIQGGAGTSTNMNANEVIANRALEI
CCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHH
LGKRKGEYQFVHPNNHVNCSQSTNDAYPTAFRIALHAKLGQLIAELRRLQQAFAAKGREF
HHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ADVLKMGRTQLQDAVPMSLGQEFDAYATTLGEDVQRLEEARALVLEVNMGATAIGTGINA
HHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEECCCCCC
PPRYTELVTRRLREIVGTPLVSSENLIEATWDTGAYVQIAGVLKRVAVKTSKICSDLRLL
CCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SSGPRTGLGEINLPPLQPGSSIMPGKVNPVMPEVVNQICYYAIGADLTVTLAAENGQLEL
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCEEEE
NVMEPVIGYGLFTSISFMVNGFRVLREKCVDGITANRERCRELVVNSIGIVTALNPIIGY
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
EASAAVAREALVTGRSVAELVLEKQLVSKELLDEVLSTENLLRPRYINPRLQKVA
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]