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The map label for this gene is trmD

Identifier: 220917154

GI number: 220917154

Start: 2289795

End: 2290538

Strand: Direct

Name: trmD

Synonym: A2cp1_2051

Alternate gene names: 220917154

Gene position: 2289795-2290538 (Clockwise)

Preceding gene: 220917153

Following gene: 220917155

Centisome position: 45.53

GC content: 74.19

Gene sequence:

>744_bases
GTGGCGAGGCTCGAGGTCGACATCCTGACGCTGTTCCCGCGCATGTGCGCGGGGTACCTGGGGGAGAGCATCCTCGGGAA
GGCGCAGGAGGCCGGCCTGCTCGCGGCGACGATCACGGACATCCGCGACCACGCGACGGGCAAGCACCGGGTCTGCGACG
ACGCGCCGTACGGCGGCGGGGCGGGCATGGTGATGAAGCCGGAGCCGCTCGTCGAGGCCATCGAGGCCGCACGCGCGCGG
CTCCCGGGGGCCTGGGTGGTCCTGACCAGCCCGCGCGGCGCGCGGCTGGATCAGGCGCTCGCGCGGCGGTTCGCGGAGCA
CGGGCGGCTGATCCTGGCGTGCGGGCGGTACGAGGGAGTGGACGAGCGGGTGATGACGGCGGTGGACATGCAGGTCTCGA
TCGGAGACTTCGTCCTCACGGGCGGCGAGCTGGCGGCGCTCTGCGTCGTCGACGCCGCCGCGCGCCTCGTCCCCGGCGTG
CTGGGCAACGCGGCGTCCGCGGACGCCGAGAGCTTCGCGGGGGTCGAGGGGCTCCTCGAGCACCCGCAGTACACGAGGCC
CCCGGAGTTCCGGGGGATGAAGGTTCCGGAGGTCCTCCTGTCCGGGGACCACCGGCGGATCGAGCGGTGGCGCCGGCGCG
AGGCGCTCAGGGCCACCCGGGAGCGGAGGCCGGACCTCTTCGCGAGGCTCTCGCTTCCGGAGAGCGACCTCCGGCTCATC
GAGGCCGGGGACGACGAACTGTAG

Upstream 100 bases:

>100_bases
GTGGATCGCGCGGCGGGTCGCGTGGTGGTGGACCCGCCGGAAGGGCTCCTGGAGCCGTAGGCCGGGGGCGAGAGGAGGCC
GCCGAGGAAGAGGATCGGAC

Downstream 100 bases:

>100_bases
GCGAGCGGTAAAGGAGACGTACGTCATGCTGCGCAAGGCGATTGCCGACATCGAGGCGAAGTACCTGCGGACCGACCTCC
CCGAGATGCGTGCGGGCGAC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MARLEVDILTLFPRMCAGYLGESILGKAQEAGLLAATITDIRDHATGKHRVCDDAPYGGGAGMVMKPEPLVEAIEAARAR
LPGAWVVLTSPRGARLDQALARRFAEHGRLILACGRYEGVDERVMTAVDMQVSIGDFVLTGGELAALCVVDAAARLVPGV
LGNAASADAESFAGVEGLLEHPQYTRPPEFRGMKVPEVLLSGDHRRIERWRRREALRATRERRPDLFARLSLPESDLRLI
EAGDDEL

Sequences:

>Translated_247_residues
MARLEVDILTLFPRMCAGYLGESILGKAQEAGLLAATITDIRDHATGKHRVCDDAPYGGGAGMVMKPEPLVEAIEAARAR
LPGAWVVLTSPRGARLDQALARRFAEHGRLILACGRYEGVDERVMTAVDMQVSIGDFVLTGGELAALCVVDAAARLVPGV
LGNAASADAESFAGVEGLLEHPQYTRPPEFRGMKVPEVLLSGDHRRIERWRRREALRATRERRPDLFARLSLPESDLRLI
EAGDDEL
>Mature_246_residues
ARLEVDILTLFPRMCAGYLGESILGKAQEAGLLAATITDIRDHATGKHRVCDDAPYGGGAGMVMKPEPLVEAIEAARARL
PGAWVVLTSPRGARLDQALARRFAEHGRLILACGRYEGVDERVMTAVDMQVSIGDFVLTGGELAALCVVDAAARLVPGVL
GNAASADAESFAGVEGLLEHPQYTRPPEFRGMKVPEVLLSGDHRRIERWRRREALRATRERRPDLFARLSLPESDLRLIE
AGDDEL

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=237, Percent_Identity=47.6793248945148, Blast_Score=201, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_ANAD2 (B8J893)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002492458.1
- ProteinModelPortal:   B8J893
- SMR:   B8J893
- GeneID:   7300178
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_2051
- HOGENOM:   HBG285805
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26861; Mature: 26730

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLEVDILTLFPRMCAGYLGESILGKAQEAGLLAATITDIRDHATGKHRVCDDAPYGGG
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
AGMVMKPEPLVEAIEAARARLPGAWVVLTSPRGARLDQALARRFAEHGRLILACGRYEGV
CCCEECCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
DERVMTAVDMQVSIGDFVLTGGELAALCVVDAAARLVPGVLGNAASADAESFAGVEGLLE
HHHHHHHHHCEEECCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
HPQYTRPPEFRGMKVPEVLLSGDHRRIERWRRREALRATRERRPDLFARLSLPESDLRLI
CCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHCEEE
EAGDDEL
ECCCCCC
>Mature Secondary Structure 
ARLEVDILTLFPRMCAGYLGESILGKAQEAGLLAATITDIRDHATGKHRVCDDAPYGGG
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
AGMVMKPEPLVEAIEAARARLPGAWVVLTSPRGARLDQALARRFAEHGRLILACGRYEGV
CCCEECCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
DERVMTAVDMQVSIGDFVLTGGELAALCVVDAAARLVPGVLGNAASADAESFAGVEGLLE
HHHHHHHHHCEEECCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
HPQYTRPPEFRGMKVPEVLLSGDHRRIERWRRREALRATRERRPDLFARLSLPESDLRLI
CCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHCEEE
EAGDDEL
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA