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The map label for this gene is pdhB [H]

Identifier: 220916930

GI number: 220916930

Start: 2039935

End: 2041593

Strand: Reverse

Name: pdhB [H]

Synonym: A2cp1_1826

Alternate gene names: 220916930

Gene position: 2041593-2039935 (Counterclockwise)

Preceding gene: 220916931

Following gene: 220916928

Centisome position: 40.59

GC content: 74.5

Gene sequence:

>1659_bases
ATGCGCACCATCGAGGTCAAGGTCCCGAACATCGGCGACTACAAGGACGTCCCGGTCATCGACGTGCTGGTGAAGCCCGG
CGAGCAGGTGGACGCCGACGCGCCGCTGGTCACGCTCGAGTCGGACAAGGCCACGCTCGACGTGCCCGCCCCCGCGGCCG
GCACCATCCGCGAGGTGAAGGTGAAGGTCGGCGACCGCGTCAGCGAGGGATCGCTGGTCGTGACCCTGGAGGCGTCGGAC
GGGCAGGCGAAGGCGGAGGCGCCCGCGGCGCCCGCGCAGGCCGCGCCCCCGAAGCCCGCCGCCGCGGCGCCCGCGCCGGC
GCCCTCCCCTGCCCCGGCTGCGCCCTCCGCGCCTCCGGCCGCGGCCTCGCCGGCGGGCGCGCCGCGCACGGTGGAGGTGA
AGGTCCCGAACATCGGCGACTTCAAGGACGTCCCGGTCATCGAGGTGCTGGTGAAGCCGGGCGATCGGGTCGAGGCCGAC
GCGCCGCTCGCCACGCTCGAGTCGGAGAAGGCCACGCTCGACGTGCCCGCGCCCGCGGCCGGCACGATCCGCGAGGTGGC
GCTGAAGGCCGGGGACAAGGTGAGCGAGGGATCGCTGGTGGCGATCCTCGACGCGGTCGCGCCCGCCGCCGCGACGACCG
CCGCCGCGCCGGCCCCGTCCCAGCCCGCCGCGGCCCCCGCGGCGAAGGCCGCCGCGCCGCCGCCGGCGCCCTCCCCCGCT
GCGCCGCCGGTCGCGCCGGAGACCGGTGCCCAGGGCCAGGTGCCGCACGCCTCGCCCTCGGTCCGCAAGCTCGCCCGCGA
GCTGGGCGTGAACCTCGGCCGCGTCGAGGGGAGCGGCCCGCGCGGCCGCATCCTGCAGGACGACGTGCAGAAGTTCGTGA
AGGCCTCGCTCGCCCGGCTCGAGTCCGGCGGCGGCGGGGGCGGCGCGCTCGACCTCGCGCCCTGGCCCAAGGTGGACTTC
ACCCGCTTCGGCCCGGTCGAGCGCCAGCCGCTCTCGCGCATCCGCAAGCTGTCGCGGACCAACCTGGCGCGGAACTGGGT
GATGATCCCGCACGTCACCCAGTTCGACGAGGCGGACATCACCGAGCTGGAGCGCTTCCGCGTCGAGCTGAACCGCGAGC
ACGAGAAGCAGGGCGTCAAGGTGACGCTGCTCGCGTTCCTGGTGAAGGCCTGCGTCGCCGCCATGAGGCGGTTCCCCGAG
CTGAACGCCTCGCTCGACGGCGACGAGCTGGTGCTGAAGCGCTACTTCCACGTCGGCTTCGCGGCCGACACGCCGCAGGG
GCTGGTGGTCCCGGTGCTGAAGGACGCCGATCAGAAGGGCGTCCTGCAGCTCGCGAAGGAGCTGGGCGAGCTCGCCGCGA
AGGCCCGCGACGGGAAGCTCTCCCCCGCCGACGTGCAGGGCGGCTGCTTCTCCATCTCCAGCCTGGGCGGCATCGGCGGC
ACCGCCTTCACGCCCATCATCAACGCGCCGGAGGTCGCCATCCTGGGCGTATCGCGGAGCGCCATGCGCCCGGTGTGGGA
CGGCACGCAGTTCCAGCCCCGGCTGATGCTGCCGCTGTCGCTCTCCTACGACCACCGCGTCATCGACGGCGCGCTGGCGG
CGCGGATCACGACGTACCTGGCCCAGCTCCTCGGGGATATGCGGCGGATCGTGCTCTAG

Upstream 100 bases:

>100_bases
GCACCGTGCCCGCCGCGAAGGTCGCCGAGGCGCTGAAGAAGTACGGCATCGACCCCGCGAAGCCCGCCCCCTGGACCGTC
TAGACCGCGAGAACGAGGAC

Downstream 100 bases:

>100_bases
AGCCCGTCCCTCGACTCCGGCCGTGCCCCGTCACGTCCGCTCACCCCGAGCGTCCCTCGACTCCGGCCGTGCCCCGTCAC
GTCCGCTCACCCCGAGCGTA

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MRTIEVKVPNIGDYKDVPVIDVLVKPGEQVDADAPLVTLESDKATLDVPAPAAGTIREVKVKVGDRVSEGSLVVTLEASD
GQAKAEAPAAPAQAAPPKPAAAAPAPAPSPAPAAPSAPPAAASPAGAPRTVEVKVPNIGDFKDVPVIEVLVKPGDRVEAD
APLATLESEKATLDVPAPAAGTIREVALKAGDKVSEGSLVAILDAVAPAAATTAAAPAPSQPAAAPAAKAAAPPPAPSPA
APPVAPETGAQGQVPHASPSVRKLARELGVNLGRVEGSGPRGRILQDDVQKFVKASLARLESGGGGGGALDLAPWPKVDF
TRFGPVERQPLSRIRKLSRTNLARNWVMIPHVTQFDEADITELERFRVELNREHEKQGVKVTLLAFLVKACVAAMRRFPE
LNASLDGDELVLKRYFHVGFAADTPQGLVVPVLKDADQKGVLQLAKELGELAAKARDGKLSPADVQGGCFSISSLGGIGG
TAFTPIINAPEVAILGVSRSAMRPVWDGTQFQPRLMLPLSLSYDHRVIDGALAARITTYLAQLLGDMRRIVL

Sequences:

>Translated_552_residues
MRTIEVKVPNIGDYKDVPVIDVLVKPGEQVDADAPLVTLESDKATLDVPAPAAGTIREVKVKVGDRVSEGSLVVTLEASD
GQAKAEAPAAPAQAAPPKPAAAAPAPAPSPAPAAPSAPPAAASPAGAPRTVEVKVPNIGDFKDVPVIEVLVKPGDRVEAD
APLATLESEKATLDVPAPAAGTIREVALKAGDKVSEGSLVAILDAVAPAAATTAAAPAPSQPAAAPAAKAAAPPPAPSPA
APPVAPETGAQGQVPHASPSVRKLARELGVNLGRVEGSGPRGRILQDDVQKFVKASLARLESGGGGGGALDLAPWPKVDF
TRFGPVERQPLSRIRKLSRTNLARNWVMIPHVTQFDEADITELERFRVELNREHEKQGVKVTLLAFLVKACVAAMRRFPE
LNASLDGDELVLKRYFHVGFAADTPQGLVVPVLKDADQKGVLQLAKELGELAAKARDGKLSPADVQGGCFSISSLGGIGG
TAFTPIINAPEVAILGVSRSAMRPVWDGTQFQPRLMLPLSLSYDHRVIDGALAARITTYLAQLLGDMRRIVL
>Mature_552_residues
MRTIEVKVPNIGDYKDVPVIDVLVKPGEQVDADAPLVTLESDKATLDVPAPAAGTIREVKVKVGDRVSEGSLVVTLEASD
GQAKAEAPAAPAQAAPPKPAAAAPAPAPSPAPAAPSAPPAAASPAGAPRTVEVKVPNIGDFKDVPVIEVLVKPGDRVEAD
APLATLESEKATLDVPAPAAGTIREVALKAGDKVSEGSLVAILDAVAPAAATTAAAPAPSQPAAAPAAKAAAPPPAPSPA
APPVAPETGAQGQVPHASPSVRKLARELGVNLGRVEGSGPRGRILQDDVQKFVKASLARLESGGGGGGALDLAPWPKVDF
TRFGPVERQPLSRIRKLSRTNLARNWVMIPHVTQFDEADITELERFRVELNREHEKQGVKVTLLAFLVKACVAAMRRFPE
LNASLDGDELVLKRYFHVGFAADTPQGLVVPVLKDADQKGVLQLAKELGELAAKARDGKLSPADVQGGCFSISSLGGIGG
TAFTPIINAPEVAILGVSRSAMRPVWDGTQFQPRLMLPLSLSYDHRVIDGALAARITTYLAQLLGDMRRIVL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=414, Percent_Identity=31.6425120772947, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI110671329, Length=446, Percent_Identity=29.8206278026906, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI203098816, Length=493, Percent_Identity=25.9634888438134, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=26.1640798226164, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI19923748, Length=221, Percent_Identity=33.4841628959276, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=38.125, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1786305, Length=554, Percent_Identity=47.8339350180505, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1786946, Length=402, Percent_Identity=32.8358208955224, Blast_Score=196, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17537937, Length=414, Percent_Identity=28.5024154589372, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=402, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=433, Percent_Identity=30.4849884526559, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=314, Percent_Identity=31.8471337579618, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=402, Percent_Identity=28.1094527363184, Blast_Score=163, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6324258, Length=422, Percent_Identity=27.7251184834123, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI18859875, Length=436, Percent_Identity=31.651376146789, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=35.0649350649351, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI20129315, Length=231, Percent_Identity=35.0649350649351, Blast_Score=143, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24645909, Length=205, Percent_Identity=33.6585365853659, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 57234; Mature: 57234

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIEVKVPNIGDYKDVPVIDVLVKPGEQVDADAPLVTLESDKATLDVPAPAAGTIREVK
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCEEEE
VKVGDRVSEGSLVVTLEASDGQAKAEAPAAPAQAAPPKPAAAAPAPAPSPAPAAPSAPPA
EEECCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AASPAGAPRTVEVKVPNIGDFKDVPVIEVLVKPGDRVEADAPLATLESEKATLDVPAPAA
CCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCHHEECCCCCEEECCCCCC
GTIREVALKAGDKVSEGSLVAILDAVAPAAATTAAAPAPSQPAAAPAAKAAAPPPAPSPA
HHHHHHHHHCCCCCCCCCEEEEHHHHCCHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCC
APPVAPETGAQGQVPHASPSVRKLARELGVNLGRVEGSGPRGRILQDDVQKFVKASLARL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
ESGGGGGGALDLAPWPKVDFTRFGPVERQPLSRIRKLSRTNLARNWVMIPHVTQFDEADI
HCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCH
TELERFRVELNREHEKQGVKVTLLAFLVKACVAAMRRFPELNASLDGDELVLKRYFHVGF
HHHHHHHHHHCHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
AADTPQGLVVPVLKDADQKGVLQLAKELGELAAKARDGKLSPADVQGGCFSISSLGGIGG
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHCCCCCCC
TAFTPIINAPEVAILGVSRSAMRPVWDGTQFQPRLMLPLSLSYDHRVIDGALAARITTYL
CHHCCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
AQLLGDMRRIVL
HHHHHHHHHHCC
>Mature Secondary Structure
MRTIEVKVPNIGDYKDVPVIDVLVKPGEQVDADAPLVTLESDKATLDVPAPAAGTIREVK
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCEEEE
VKVGDRVSEGSLVVTLEASDGQAKAEAPAAPAQAAPPKPAAAAPAPAPSPAPAAPSAPPA
EEECCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AASPAGAPRTVEVKVPNIGDFKDVPVIEVLVKPGDRVEADAPLATLESEKATLDVPAPAA
CCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCHHEECCCCCEEECCCCCC
GTIREVALKAGDKVSEGSLVAILDAVAPAAATTAAAPAPSQPAAAPAAKAAAPPPAPSPA
HHHHHHHHHCCCCCCCCCEEEEHHHHCCHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCC
APPVAPETGAQGQVPHASPSVRKLARELGVNLGRVEGSGPRGRILQDDVQKFVKASLARL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
ESGGGGGGALDLAPWPKVDFTRFGPVERQPLSRIRKLSRTNLARNWVMIPHVTQFDEADI
HCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCH
TELERFRVELNREHEKQGVKVTLLAFLVKACVAAMRRFPELNASLDGDELVLKRYFHVGF
HHHHHHHHHHCHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
AADTPQGLVVPVLKDADQKGVLQLAKELGELAAKARDGKLSPADVQGGCFSISSLGGIGG
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHCCCCCCC
TAFTPIINAPEVAILGVSRSAMRPVWDGTQFQPRLMLPLSLSYDHRVIDGALAARITTYL
CHHCCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
AQLLGDMRRIVL
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]