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The map label for this gene is thiG

Identifier: 220916539

GI number: 220916539

Start: 1604370

End: 1605143

Strand: Direct

Name: thiG

Synonym: A2cp1_1433

Alternate gene names: 220916539

Gene position: 1604370-1605143 (Clockwise)

Preceding gene: 220916538

Following gene: 220916541

Centisome position: 31.9

GC content: 71.32

Gene sequence:

>774_bases
ATGGCGGACACCTGGAGCATCGGCGCGCACGCGTTCACCAGCCGCCTGCTGGTGGGCACGGGCAAGTACCCCGACTTCCC
CACCATGCAGCGGGCGCTCGCCGCCTCGGGCGCGGAGGTCGTCACCGTCGCGGTCCGCCGGCTCGACCTGTCGAAGAAGG
GCGAGGAGTCGCTGCTCGCCTGGATCCCGAAGGGCATGAAGCTCCTGCCCAACACCGCCGCCTGCTTCACCGCCGAGGAG
GCCATCCGCACCGCAAGGCTCGGCCGCGAGCTGGAGATGGGCGATCTGGTGAAGCTGGAGGTGATCGGCGACCGCCGGAC
GCTGTTCCCCGACGTGGAGGGGCTGATCCAGGCGGCGAAGGTGCTGGTGAAGGAGGGCTTCACCGTCCTGCCGTACACGA
ACGACGACCCGGTCACCGCGAAGAAGCTCGAGGACGCGGGCTGCGCCGCGGTGATGCCGCTCGGGGCTCCCATCGGCTCC
GGCCTCGGCCTCCGCAACCCGTACAACCTCCGCATCATCATGGAGACGGTGCAGGTGCCGGTGCTGGTGGACGCGGGCGT
GGGCACGGCCAGCGACGCGGCGCTCGCGATGGAGCTGGGCGCGGTCGCGGTGCTCATGAACACCGCCATCGCCGAGGCGA
AGGATCCGGTGCTCATGGCCGAGGCCATGCGCGCCGGCGTGGAGGGCGGCCGCAAGGCGTTCCTCGCCGGCCGGATCCCG
ATGAAGCTGCACGCCGCGGCGTCCTCGCCGATGAGCGGGCTCATCGGGAGCTGA

Upstream 100 bases:

>100_bases
GGTCAACGAGGCGGTCGTCACCAAGGACCGCTACGAGGCCCACCGGATCGGTCCGGGCGACTCGGTCGAGATCGTCGCCT
TCGTCGGAGGAGGGTAGGCC

Downstream 100 bases:

>100_bases
CGGCCGGGCGCTCCCTCGCCTCCTCTCGACGGTGCGCCGCCGAGGCGACGCCGCGCTCCGGGCGAGCGCGGGCGAGCCGG
AGGCGGTCGCCTGGATCGAG

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MADTWSIGAHAFTSRLLVGTGKYPDFPTMQRALAASGAEVVTVAVRRLDLSKKGEESLLAWIPKGMKLLPNTAACFTAEE
AIRTARLGRELEMGDLVKLEVIGDRRTLFPDVEGLIQAAKVLVKEGFTVLPYTNDDPVTAKKLEDAGCAAVMPLGAPIGS
GLGLRNPYNLRIIMETVQVPVLVDAGVGTASDAALAMELGAVAVLMNTAIAEAKDPVLMAEAMRAGVEGGRKAFLAGRIP
MKLHAAASSPMSGLIGS

Sequences:

>Translated_257_residues
MADTWSIGAHAFTSRLLVGTGKYPDFPTMQRALAASGAEVVTVAVRRLDLSKKGEESLLAWIPKGMKLLPNTAACFTAEE
AIRTARLGRELEMGDLVKLEVIGDRRTLFPDVEGLIQAAKVLVKEGFTVLPYTNDDPVTAKKLEDAGCAAVMPLGAPIGS
GLGLRNPYNLRIIMETVQVPVLVDAGVGTASDAALAMELGAVAVLMNTAIAEAKDPVLMAEAMRAGVEGGRKAFLAGRIP
MKLHAAASSPMSGLIGS
>Mature_256_residues
ADTWSIGAHAFTSRLLVGTGKYPDFPTMQRALAASGAEVVTVAVRRLDLSKKGEESLLAWIPKGMKLLPNTAACFTAEEA
IRTARLGRELEMGDLVKLEVIGDRRTLFPDVEGLIQAAKVLVKEGFTVLPYTNDDPVTAKKLEDAGCAAVMPLGAPIGSG
LGLRNPYNLRIIMETVQVPVLVDAGVGTASDAALAMELGAVAVLMNTAIAEAKDPVLMAEAMRAGVEGGRKAFLAGRIPM
KLHAAASSPMSGLIGS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=249, Percent_Identity=48.5943775100402, Blast_Score=212, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_ANAD2 (B8JH75)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002491843.1
- ProteinModelPortal:   B8JH75
- SMR:   B8JH75
- GeneID:   7299954
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_1433
- HOGENOM:   HBG296821
- ProtClustDB:   PRK00208
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 26926; Mature: 26795

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: ACT_SITE 98-98 BINDING 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTWSIGAHAFTSRLLVGTGKYPDFPTMQRALAASGAEVVTVAVRRLDLSKKGEESLLA
CCCCCCCCHHHHHHHHEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHH
WIPKGMKLLPNTAACFTAEEAIRTARLGRELEMGDLVKLEVIGDRRTLFPDVEGLIQAAK
HCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
VLVKEGFTVLPYTNDDPVTAKKLEDAGCAAVMPLGAPIGSGLGLRNPYNLRIIMETVQVP
HHHHCCCEEEECCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCC
VLVDAGVGTASDAALAMELGAVAVLMNTAIAEAKDPVLMAEAMRAGVEGGRKAFLAGRIP
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCC
MKLHAAASSPMSGLIGS
EEEEECCCCCCCCCCCC
>Mature Secondary Structure 
ADTWSIGAHAFTSRLLVGTGKYPDFPTMQRALAASGAEVVTVAVRRLDLSKKGEESLLA
CCCCCCCHHHHHHHHEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHH
WIPKGMKLLPNTAACFTAEEAIRTARLGRELEMGDLVKLEVIGDRRTLFPDVEGLIQAAK
HCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
VLVKEGFTVLPYTNDDPVTAKKLEDAGCAAVMPLGAPIGSGLGLRNPYNLRIIMETVQVP
HHHHCCCEEEECCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCC
VLVDAGVGTASDAALAMELGAVAVLMNTAIAEAKDPVLMAEAMRAGVEGGRKAFLAGRIP
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCC
MKLHAAASSPMSGLIGS
EEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA