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The map label for this gene is thiE [H]

Identifier: 220916541

GI number: 220916541

Start: 1606105

End: 1606716

Strand: Direct

Name: thiE [H]

Synonym: A2cp1_1435

Alternate gene names: 220916541

Gene position: 1606105-1606716 (Clockwise)

Preceding gene: 220916539

Following gene: 220916543

Centisome position: 31.93

GC content: 79.08

Gene sequence:

>612_bases
ATGGAGGTCCCCGTTGTCCACCTGATCACGGACCGTCGGCTCGCTTCCTCGCTGCCGGCGCGCGCCGCCGCCGCCCTGCG
CGGGCTGCCCCCCGGGATCGCCGCGATCCACCTGCGAGAGAAGGACCTTTGCGGTCTGGATTTGCTCCGGCTCGCGCGCG
CGCTCGCCGCGGTCTGCCGCGACGCGGGCCAGCGGCTGCTCGTGAACGATCGGCTGGACGTGGCGCTCGCAGCCGGGGCC
GACGGCGTCCACCTCCCGTCCGCGGGCATCTCGCCGGTGGACGCGCGGCGGCTGCTCGGCCCCGCGGCCCTGCTGGGCGT
CTCGTGCCACTCGGAGGCGGACGTGGTCCGCGCCCGCGCGGGCGGGGCGAGCTTCGCCACGTTCGGGCCCGTCTACGACA
CGCCCTCGAAGCGCCCCTACGGCGCGCCGGTGGGCGTCGGCGCGCTGCGCGAGGCGGCGCGGCTCGGGCTCCCGCTGGTC
GCGCTCGGCGGGGTGGACCCCTCGCGCGTCGCCGAGGTGCGCGCCGCGGGCGCGCGCGGGGTCGCGGCCATCCGCGCCTG
GCTCACCGGCGACGATCCCGCCGGCGCGGTGCGCGCGCTCCTCGGCCGCTGA

Upstream 100 bases:

>100_bases
GCTTCGCCTTCTGCGCTTCCAGGAAGGCGAGGAAGCTCTGGAGATCGCTCGACACGGATGCTCTCCTCGCGCAGGATGTT
GCCGGAGGCGGAGATTACGG

Downstream 100 bases:

>100_bases
TCCCGGCGCGCGGCGGGCTCAGCCGGCGCGCCGCAGCGACAGGAAGTACTGGTACGGCAGGAACGCGTGCTCCTCTGCCA
GCTCCAGCCCGGCCTGGTGC

Product: Thiamine-phosphate diphosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MEVPVVHLITDRRLASSLPARAAAALRGLPPGIAAIHLREKDLCGLDLLRLARALAAVCRDAGQRLLVNDRLDVALAAGA
DGVHLPSAGISPVDARRLLGPAALLGVSCHSEADVVRARAGGASFATFGPVYDTPSKRPYGAPVGVGALREAARLGLPLV
ALGGVDPSRVAEVRAAGARGVAAIRAWLTGDDPAGAVRALLGR

Sequences:

>Translated_203_residues
MEVPVVHLITDRRLASSLPARAAAALRGLPPGIAAIHLREKDLCGLDLLRLARALAAVCRDAGQRLLVNDRLDVALAAGA
DGVHLPSAGISPVDARRLLGPAALLGVSCHSEADVVRARAGGASFATFGPVYDTPSKRPYGAPVGVGALREAARLGLPLV
ALGGVDPSRVAEVRAAGARGVAAIRAWLTGDDPAGAVRALLGR
>Mature_203_residues
MEVPVVHLITDRRLASSLPARAAAALRGLPPGIAAIHLREKDLCGLDLLRLARALAAVCRDAGQRLLVNDRLDVALAAGA
DGVHLPSAGISPVDARRLLGPAALLGVSCHSEADVVRARAGGASFATFGPVYDTPSKRPYGAPVGVGALREAARLGLPLV
ALGGVDPSRVAEVRAAGARGVAAIRAWLTGDDPAGAVRALLGR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=162, Percent_Identity=31.4814814814815, Blast_Score=61, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6325042, Length=182, Percent_Identity=28.021978021978, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 20651; Mature: 20651

Theoretical pI: Translated: 10.77; Mature: 10.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVPVVHLITDRRLASSLPARAAAALRGLPPGIAAIHLREKDLCGLDLLRLARALAAVCR
CCCCEEEEECCHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
DAGQRLLVNDRLDVALAAGADGVHLPSAGISPVDARRLLGPAALLGVSCHSEADVVRARA
HCCCEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHCHHHHHCCCCCCCHHHHHHHC
GGASFATFGPVYDTPSKRPYGAPVGVGALREAARLGLPLVALGGVDPSRVAEVRAAGARG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCC
VAAIRAWLTGDDPAGAVRALLGR
HHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MEVPVVHLITDRRLASSLPARAAAALRGLPPGIAAIHLREKDLCGLDLLRLARALAAVCR
CCCCEEEEECCHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
DAGQRLLVNDRLDVALAAGADGVHLPSAGISPVDARRLLGPAALLGVSCHSEADVVRARA
HCCCEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHCHHHHHCCCCCCCHHHHHHHC
GGASFATFGPVYDTPSKRPYGAPVGVGALREAARLGLPLVALGGVDPSRVAEVRAAGARG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCC
VAAIRAWLTGDDPAGAVRALLGR
HHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA