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The map label for this gene is purB [H]

Identifier: 220916343

GI number: 220916343

Start: 1388221

End: 1389540

Strand: Direct

Name: purB [H]

Synonym: A2cp1_1237

Alternate gene names: 220916343

Gene position: 1388221-1389540 (Clockwise)

Preceding gene: 220916342

Following gene: 220916344

Centisome position: 27.6

GC content: 72.12

Gene sequence:

>1320_bases
ATGATCCCGCGCTACACCCGGCCCGACATGGGCCGCATCTGGACTTCCGAGCACCGCTTCCGCATCTGGCTCGACGTGGA
GCTGGCCGCGTGCGAGGCGATGGTGCGCCTGGGCGAGGTGCCGCCCGAGGACTTCGAGGCGCTCCGCAAGGCGTTCGCCG
GCTTCGAGTTCACCGCCGCGGACGTGGCCCGCATCGACGAGATCGAGCGGACCGTGAAGCACGACGTGATCGCCTTCCTC
ACGTTCGTGGAGGAGAAGGGCGGCCCGGCCGCGCGCCACCTGCACAAGGGCATGACCTCGTCGGACGTGCTCGACACGAC
GCTCGCGGTGCAGCTCCGCGACGCGAGCCGGCTGCTGCTCGCCGGCGTGGACCGGGTGCTCGCCGCGGTCCGCAAGCGGG
CCTTCGAGCACCGGCGCACGCCGATGGTGGGCCGGAGCCACGGCATCCACGCCGAGCCGATCACGTTCGGGCTGAAGCTC
GCCGGCTGGTACGACGCCTGGACCCGCCGCCGCGCCGCGCTGGCCCGCGCCGCCGAGGGGGTGGCGGTCGGCAAGATCTC
CGGCGCGGTGGGCACGTTCGCGAACGTGGACCCGCGGGTGGAGGCGTTCGTGATGGAGAAGGTCGGCCTCGCCGGCGGCG
AGGGCGCGGCCACGCAGGTCGTGAACCGCGACCGCCACGCCGAGCTGTTCACCGCGCTGGCGCTCTGCGGCGCCACGCTG
GAGCAGCAGGCGGTCGAGGTCCGGCACCTGCAGCGCACCGAGGTGCGCGAGGCGGAGGAGCCGTTCACCGCCGGGCAGAA
GGGCTCCTCGGCCATGCCGCACAAGCGCAACCCCATCCTCTCCGAGAACCTCACCGGCCTGGCCCGGCTGCTGCGCGGCT
ACGCGCTCGCCGCCATGGAGGACGTGGCGCTCTGGCACGAGCGCGACATCAGCCACTCCTCGGTGGAGCGGGTGATCGGG
CCGGACGCGACCATCGCGCTCGACTTCGCGCTGCACCGCTTCGCGGGGATGATCGAGAACCTGCGCGTGTACCCCGAGCG
CATGCGCGAGAACCTGGACCTCACCGGCGGGCTGTACGAGGCGCAGCGCGTGCTGCTGGCGCTGGTCGGCAAGGGCGTGG
CCCGCCAGCAGGGCTACGTGTTCGTCCAGCGCAACGCGATGAAGGTCTGGGAGGAGAAGGTGGACTTCCGGACTGCGCTG
AAGCGCGACCCCGACGTGAGCCGGCTGCTCACGGCCGACGAGGTCGACGCCTGCTTCGCGCTCGACTACCACCTGAAGCA
CGTGGACACCATCTTCCGCCGGGTGTTCGGCGAGGCCTGA

Upstream 100 bases:

>100_bases
GTGGTCCGGGCCGAGCTCGAGCTGGTGGACGCGCTGAGCGACAGCGACCGAGGCGCCGGCGGCTTCGGCTCGACCGGCCA
GTAGCCTCACGGAGGACGCC

Downstream 100 bases:

>100_bases
GCCGCGCCGCGCCGGGGAGGGCCCGCGCATGCTCCGCCTGGAGGAGTCGGTCGGCGCGCTCCACGTGACCTCGCGGCTGG
CGCGGCCGCGCGCGCTGGCC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MIPRYTRPDMGRIWTSEHRFRIWLDVELAACEAMVRLGEVPPEDFEALRKAFAGFEFTAADVARIDEIERTVKHDVIAFL
TFVEEKGGPAARHLHKGMTSSDVLDTTLAVQLRDASRLLLAGVDRVLAAVRKRAFEHRRTPMVGRSHGIHAEPITFGLKL
AGWYDAWTRRRAALARAAEGVAVGKISGAVGTFANVDPRVEAFVMEKVGLAGGEGAATQVVNRDRHAELFTALALCGATL
EQQAVEVRHLQRTEVREAEEPFTAGQKGSSAMPHKRNPILSENLTGLARLLRGYALAAMEDVALWHERDISHSSVERVIG
PDATIALDFALHRFAGMIENLRVYPERMRENLDLTGGLYEAQRVLLALVGKGVARQQGYVFVQRNAMKVWEEKVDFRTAL
KRDPDVSRLLTADEVDACFALDYHLKHVDTIFRRVFGEA

Sequences:

>Translated_439_residues
MIPRYTRPDMGRIWTSEHRFRIWLDVELAACEAMVRLGEVPPEDFEALRKAFAGFEFTAADVARIDEIERTVKHDVIAFL
TFVEEKGGPAARHLHKGMTSSDVLDTTLAVQLRDASRLLLAGVDRVLAAVRKRAFEHRRTPMVGRSHGIHAEPITFGLKL
AGWYDAWTRRRAALARAAEGVAVGKISGAVGTFANVDPRVEAFVMEKVGLAGGEGAATQVVNRDRHAELFTALALCGATL
EQQAVEVRHLQRTEVREAEEPFTAGQKGSSAMPHKRNPILSENLTGLARLLRGYALAAMEDVALWHERDISHSSVERVIG
PDATIALDFALHRFAGMIENLRVYPERMRENLDLTGGLYEAQRVLLALVGKGVARQQGYVFVQRNAMKVWEEKVDFRTAL
KRDPDVSRLLTADEVDACFALDYHLKHVDTIFRRVFGEA
>Mature_439_residues
MIPRYTRPDMGRIWTSEHRFRIWLDVELAACEAMVRLGEVPPEDFEALRKAFAGFEFTAADVARIDEIERTVKHDVIAFL
TFVEEKGGPAARHLHKGMTSSDVLDTTLAVQLRDASRLLLAGVDRVLAAVRKRAFEHRRTPMVGRSHGIHAEPITFGLKL
AGWYDAWTRRRAALARAAEGVAVGKISGAVGTFANVDPRVEAFVMEKVGLAGGEGAATQVVNRDRHAELFTALALCGATL
EQQAVEVRHLQRTEVREAEEPFTAGQKGSSAMPHKRNPILSENLTGLARLLRGYALAAMEDVALWHERDISHSSVERVIG
PDATIALDFALHRFAGMIENLRVYPERMRENLDLTGGLYEAQRVLLALVGKGVARQQGYVFVQRNAMKVWEEKVDFRTAL
KRDPDVSRLLTADEVDACFALDYHLKHVDTIFRRVFGEA

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=412, Percent_Identity=28.6407766990291, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI183227688, Length=389, Percent_Identity=28.7917737789203, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1787376, Length=359, Percent_Identity=28.9693593314763, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=353, Percent_Identity=27.1954674220963, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32564234, Length=270, Percent_Identity=29.2592592592593, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323391, Length=389, Percent_Identity=28.0205655526992, Blast_Score=132, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24647570, Length=425, Percent_Identity=28.2352941176471, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48953; Mature: 48953

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYTRPDMGRIWTSEHRFRIWLDVELAACEAMVRLGEVPPEDFEALRKAFAGFEFTAA
CCCCCCCCCCCCEECCCCEEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHH
DVARIDEIERTVKHDVIAFLTFVEEKGGPAARHLHKGMTSSDVLDTTLAVQLRDASRLLL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHEEECCHHHHHH
AGVDRVLAAVRKRAFEHRRTPMVGRSHGIHAEPITFGLKLAGWYDAWTRRRAALARAAEG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCC
VAVGKISGAVGTFANVDPRVEAFVMEKVGLAGGEGAATQVVNRDRHAELFTALALCGATL
CEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
EQQAVEVRHLQRTEVREAEEPFTAGQKGSSAMPHKRNPILSENLTGLARLLRGYALAAME
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DVALWHERDISHSSVERVIGPDATIALDFALHRFAGMIENLRVYPERMRENLDLTGGLYE
HHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHH
AQRVLLALVGKGVARQQGYVFVQRNAMKVWEEKVDFRTALKRDPDVSRLLTADEVDACFA
HHHHHHHHHCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHCHHHCCHHHH
LDYHLKHVDTIFRRVFGEA
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPRYTRPDMGRIWTSEHRFRIWLDVELAACEAMVRLGEVPPEDFEALRKAFAGFEFTAA
CCCCCCCCCCCCEECCCCEEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHH
DVARIDEIERTVKHDVIAFLTFVEEKGGPAARHLHKGMTSSDVLDTTLAVQLRDASRLLL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHEEECCHHHHHH
AGVDRVLAAVRKRAFEHRRTPMVGRSHGIHAEPITFGLKLAGWYDAWTRRRAALARAAEG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCC
VAVGKISGAVGTFANVDPRVEAFVMEKVGLAGGEGAATQVVNRDRHAELFTALALCGATL
CEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
EQQAVEVRHLQRTEVREAEEPFTAGQKGSSAMPHKRNPILSENLTGLARLLRGYALAAME
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DVALWHERDISHSSVERVIGPDATIALDFALHRFAGMIENLRVYPERMRENLDLTGGLYE
HHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHH
AQRVLLALVGKGVARQQGYVFVQRNAMKVWEEKVDFRTALKRDPDVSRLLTADEVDACFA
HHHHHHHHHCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHCHHHCCHHHH
LDYHLKHVDTIFRRVFGEA
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]