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The map label for this gene is pnp [H]

Identifier: 220916341

GI number: 220916341

Start: 1385570

End: 1387735

Strand: Direct

Name: pnp [H]

Synonym: A2cp1_1235

Alternate gene names: 220916341

Gene position: 1385570-1387735 (Clockwise)

Preceding gene: 220916340

Following gene: 220916342

Centisome position: 27.55

GC content: 68.98

Gene sequence:

>2166_bases
ATGACGCCCATCCAGAAGACCGCCACGGTCGGCGGCAAGGACATCCTGCTCGAGACCGGCAAGGTCGCGAAGCAGGCGCA
CGGCTCGGTCTGGGTCCGCCTCGGCGACTCGATCGTGCTCGTCACCGCGGTGTCCGCCGCGGAGAAGAAGGAAGGCATCG
ACTTCTTCCCGCTGACGGTGGACTACCAGGAGAAGCTGTTCGCCGCGGGCCGTGTCCCCGGCAGCTTCTTCCGCCGCGAG
GGCCGCCCCACCGAGAAGGAGACGCTGACCTCGCGTCTGGTGGACCGGTCCTGCCGGCCGCTGTTCGCCGAGGGCTACTC
CAACGAGACGCAGGTCATCGCGACCGTCATCTCCTTCGACCAGGAGAACGACACCGACGTGCTCGCGCTCACCGGCGCGT
CCGCGGCGCTCCACATCTCGGACATCCCGTTCGGCGGCCCGATCGCCGGCGTCCGCGTCGCCCGCGTGGGCGGCCAGCTC
GTGGCGAACCCGACGCTGGCCCAGCGCGCCGAGGCCGACCTCGACGTGGTGATGGCCGCCTCCCGCGACGCCATCGTGAT
GGTGGAGGGCGGCGCGCAGGAGGTGTCCGAGGCGGTGATGATCGAGGCGCTGCTGTTCGGGCAGGCCGCGGTCCAGCCGC
TGCTCGACGCCCAGGACGCGCTCCGCGCGGCGACCGGCAACAAGGCGCGCCGCGCCTTCGAGCCGCCCAAGAACGACGTC
GAGCTGCGCGCCAAGGTGAAGGCCCTCACCTGGGAGAAGGTGAAGGAGGCCTACGGCCGCAACGAGAAGCACGACCGGTA
CGGCCGGCTCTCGGAGATCAAGAAGGAGCTCCTCCAGGCCCTCAAGGACGAGGCGGCCGGCGACGCGGCGAAGCTCGCGA
CCATCGCCCTCCGCGAGAAGGAGATCAAGGGCTACTACGAGGACGTGAAGTACGACTACATGCGCAAGATGATCACGGAT
GAGCGCCGCCGCATCGGCGGCCGCGGCATGGCCGACATCCGCAAGATCACCTGCGAGGTGGGGCTGCTCCCGCGCGTTCA
CGGCTCGTCGCTGTTCACCCGCGGCGAGACGCAGGCGCTGGTGGCCACCACGCTCGGCACCGCCGAGGACGAGCAGCGCG
TCGAGATGCTCACCGGCATGGTCTTCAAGAAGTTCATGCTGCACTACAACTTCCCGCCGTTCTCCGTCGGCGAGGTGAAG
TTCCTGCGCAGCCCGGGCCGCCGCGAGATCGGCCACGGCGCGCTGGCCGAGCGCGCGCTCCGCGCGGTGATGCCGCCCGA
GGACCAGTTCCCCTACACGGTCCGCGTCGTCTCCGACATCATGGAGTCGAACGGCTCGTCGTCGATGGCGTCGGTGTGCG
GCGGCTGCCTCTCGCTGATGGACGCCGGCGTGCCCATCAAGGCGCCGGTGGCCGGCATCGCCATGGGCCTCATCAAGGAG
GGCGAGAAGATCGCCATCCTCTCCGACATCCTCGGGGACGAGGATCACCTCGGCGACATGGACTTCAAGGTCTGCGGCAC
CGCCGCGGGCATCACCTCGATCCAGATGGACATCAAGATCGGCGGCGTGACCCGCGACATCCTCGAGCAGGCGCTCGCGC
AGGCCGCCGAGGGCCGCAAGCACATCCTGGGCGAGATGGCGAAGGCGCTCTCCGCGCCGCGCGGCAGCATCAGCGCCTAC
GCGCCGCGCATCACGACCATCAAGATCCGGCCGGAGCGCATCAAGGACATCATCGGCCCCGGCGGCAAGACCATCAAGGA
CATCACCGCCCGCACCGGCACCTCGATCAACATCGAGGACGACGGCTCGGTCTCCATCGCGTCCCCGAACCAGGACAAGG
TGGAGGAGGCGATCAAGATGATCCGCGGCCTCACCCAGGAGGCGGAGGTCGGCCGCATCTACCTCGGCACGGTCCGCAAG
ATCGCCGAGTTCGGCGCCTTCGTGGAGATCTTCCCGGGCACCGACGGCCTCATCCACATCTCCGAGCTCTCCGACAAGCG
CGTGAAGAGCGTCTCCGACGTCCTCTCCGAGGGCGAGGAGGTGATGGTGAAGGTCATCTCGGTGGATCGCTCCGGGAAGA
TCCGCCTCTCGCGCAAGGAGGCCCTCGCCGACAGCGCCAAGAAGTCCGAGGGCACCGAGCCGCCGAAGGGCGAGCCGGCC
AAGTAG

Upstream 100 bases:

>100_bases
AACAGAGGCACCTTCGAAGGCCCGAGGGCGAACCGGCCCCGCGGGACCAGCGGGACGAACGCGGCCGCAGCGCGGCCGGC
GCGCCCCGAGGAAGGTGCAC

Downstream 100 bases:

>100_bases
GCCGCGAGGGGCCGCGAGGACGCCGATGCCCGTGACCGTCCGAGTCCGCCGCGTCGGCCATCGCGGCCCCCCGCTCGATC
TCCCCCGCTACGAGTCCGCC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MTPIQKTATVGGKDILLETGKVAKQAHGSVWVRLGDSIVLVTAVSAAEKKEGIDFFPLTVDYQEKLFAAGRVPGSFFRRE
GRPTEKETLTSRLVDRSCRPLFAEGYSNETQVIATVISFDQENDTDVLALTGASAALHISDIPFGGPIAGVRVARVGGQL
VANPTLAQRAEADLDVVMAASRDAIVMVEGGAQEVSEAVMIEALLFGQAAVQPLLDAQDALRAATGNKARRAFEPPKNDV
ELRAKVKALTWEKVKEAYGRNEKHDRYGRLSEIKKELLQALKDEAAGDAAKLATIALREKEIKGYYEDVKYDYMRKMITD
ERRRIGGRGMADIRKITCEVGLLPRVHGSSLFTRGETQALVATTLGTAEDEQRVEMLTGMVFKKFMLHYNFPPFSVGEVK
FLRSPGRREIGHGALAERALRAVMPPEDQFPYTVRVVSDIMESNGSSSMASVCGGCLSLMDAGVPIKAPVAGIAMGLIKE
GEKIAILSDILGDEDHLGDMDFKVCGTAAGITSIQMDIKIGGVTRDILEQALAQAAEGRKHILGEMAKALSAPRGSISAY
APRITTIKIRPERIKDIIGPGGKTIKDITARTGTSINIEDDGSVSIASPNQDKVEEAIKMIRGLTQEAEVGRIYLGTVRK
IAEFGAFVEIFPGTDGLIHISELSDKRVKSVSDVLSEGEEVMVKVISVDRSGKIRLSRKEALADSAKKSEGTEPPKGEPA
K

Sequences:

>Translated_721_residues
MTPIQKTATVGGKDILLETGKVAKQAHGSVWVRLGDSIVLVTAVSAAEKKEGIDFFPLTVDYQEKLFAAGRVPGSFFRRE
GRPTEKETLTSRLVDRSCRPLFAEGYSNETQVIATVISFDQENDTDVLALTGASAALHISDIPFGGPIAGVRVARVGGQL
VANPTLAQRAEADLDVVMAASRDAIVMVEGGAQEVSEAVMIEALLFGQAAVQPLLDAQDALRAATGNKARRAFEPPKNDV
ELRAKVKALTWEKVKEAYGRNEKHDRYGRLSEIKKELLQALKDEAAGDAAKLATIALREKEIKGYYEDVKYDYMRKMITD
ERRRIGGRGMADIRKITCEVGLLPRVHGSSLFTRGETQALVATTLGTAEDEQRVEMLTGMVFKKFMLHYNFPPFSVGEVK
FLRSPGRREIGHGALAERALRAVMPPEDQFPYTVRVVSDIMESNGSSSMASVCGGCLSLMDAGVPIKAPVAGIAMGLIKE
GEKIAILSDILGDEDHLGDMDFKVCGTAAGITSIQMDIKIGGVTRDILEQALAQAAEGRKHILGEMAKALSAPRGSISAY
APRITTIKIRPERIKDIIGPGGKTIKDITARTGTSINIEDDGSVSIASPNQDKVEEAIKMIRGLTQEAEVGRIYLGTVRK
IAEFGAFVEIFPGTDGLIHISELSDKRVKSVSDVLSEGEEVMVKVISVDRSGKIRLSRKEALADSAKKSEGTEPPKGEPA
K
>Mature_720_residues
TPIQKTATVGGKDILLETGKVAKQAHGSVWVRLGDSIVLVTAVSAAEKKEGIDFFPLTVDYQEKLFAAGRVPGSFFRREG
RPTEKETLTSRLVDRSCRPLFAEGYSNETQVIATVISFDQENDTDVLALTGASAALHISDIPFGGPIAGVRVARVGGQLV
ANPTLAQRAEADLDVVMAASRDAIVMVEGGAQEVSEAVMIEALLFGQAAVQPLLDAQDALRAATGNKARRAFEPPKNDVE
LRAKVKALTWEKVKEAYGRNEKHDRYGRLSEIKKELLQALKDEAAGDAAKLATIALREKEIKGYYEDVKYDYMRKMITDE
RRRIGGRGMADIRKITCEVGLLPRVHGSSLFTRGETQALVATTLGTAEDEQRVEMLTGMVFKKFMLHYNFPPFSVGEVKF
LRSPGRREIGHGALAERALRAVMPPEDQFPYTVRVVSDIMESNGSSSMASVCGGCLSLMDAGVPIKAPVAGIAMGLIKEG
EKIAILSDILGDEDHLGDMDFKVCGTAAGITSIQMDIKIGGVTRDILEQALAQAAEGRKHILGEMAKALSAPRGSISAYA
PRITTIKIRPERIKDIIGPGGKTIKDITARTGTSINIEDDGSVSIASPNQDKVEEAIKMIRGLTQEAEVGRIYLGTVRKI
AEFGAFVEIFPGTDGLIHISELSDKRVKSVSDVLSEGEEVMVKVISVDRSGKIRLSRKEALADSAKKSEGTEPPKGEPAK

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=720, Percent_Identity=37.5, Blast_Score=456, Evalue=1e-128,
Organism=Escherichia coli, GI145693187, Length=705, Percent_Identity=51.7730496453901, Blast_Score=699, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=714, Percent_Identity=35.5742296918768, Blast_Score=349, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI17535281, Length=81, Percent_Identity=45.679012345679, Blast_Score=67, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320850, Length=101, Percent_Identity=39.6039603960396, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI281362905, Length=714, Percent_Identity=35.9943977591036, Blast_Score=454, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651641, Length=714, Percent_Identity=35.9943977591036, Blast_Score=454, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651643, Length=714, Percent_Identity=35.9943977591036, Blast_Score=454, Evalue=1e-127,
Organism=Drosophila melanogaster, GI161079377, Length=658, Percent_Identity=35.8662613981763, Blast_Score=414, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77860; Mature: 77729

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPIQKTATVGGKDILLETGKVAKQAHGSVWVRLGDSIVLVTAVSAAEKKEGIDFFPLTV
CCCCCCHHCCCCCCEEEECCHHHHHCCCEEEEEECCCEEEEEEHHHHHHHCCCEEEEEEE
DYQEKLFAAGRVPGSFFRREGRPTEKETLTSRLVDRSCRPLFAEGYSNETQVIATVISFD
CHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCHHHEEHHHHCC
QENDTDVLALTGASAALHISDIPFGGPIAGVRVARVGGQLVANPTLAQRAEADLDVVMAA
CCCCCCEEEEECCCEEEEEECCCCCCCHHCEEHHHCCCEEECCCHHHHHHCCCCEEEEEC
SRDAIVMVEGGAQEVSEAVMIEALLFGQAAVQPLLDAQDALRAATGNKARRAFEPPKNDV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCH
ELRAKVKALTWEKVKEAYGRNEKHDRYGRLSEIKKELLQALKDEAAGDAAKLATIALREK
HHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EIKGYYEDVKYDYMRKMITDERRRIGGRGMADIRKITCEVGLLPRVHGSSLFTRGETQAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCEE
VATTLGTAEDEQRVEMLTGMVFKKFMLHYNFPPFSVGEVKFLRSPGRREIGHGALAERAL
EEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHH
RAVMPPEDQFPYTVRVVSDIMESNGSSSMASVCGGCLSLMDAGVPIKAPVAGIAMGLIKE
HHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
GEKIAILSDILGDEDHLGDMDFKVCGTAAGITSIQMDIKIGGVTRDILEQALAQAAEGRK
CCEEEEHHHHCCCCCCCCCCCCEECCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCHH
HILGEMAKALSAPRGSISAYAPRITTIKIRPERIKDIIGPGGKTIKDITARTGTSINIED
HHHHHHHHHHCCCCCCCEECCCEEEEEEECHHHHHHHHCCCCCHHHHHHHCCCCEEEECC
DGSVSIASPNQDKVEEAIKMIRGLTQEAEVGRIYLGTVRKIAEFGAFVEIFPGTDGLIHI
CCCEEECCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCEEEEECCCCCEEEE
SELSDKRVKSVSDVLSEGEEVMVKVISVDRSGKIRLSRKEALADSAKKSEGTEPPKGEPA
HHHCHHHHHHHHHHHHCCHHEEEEEEEECCCCCEEECHHHHHHHHHHHCCCCCCCCCCCC
K
C
>Mature Secondary Structure 
TPIQKTATVGGKDILLETGKVAKQAHGSVWVRLGDSIVLVTAVSAAEKKEGIDFFPLTV
CCCCCHHCCCCCCEEEECCHHHHHCCCEEEEEECCCEEEEEEHHHHHHHCCCEEEEEEE
DYQEKLFAAGRVPGSFFRREGRPTEKETLTSRLVDRSCRPLFAEGYSNETQVIATVISFD
CHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCHHHEEHHHHCC
QENDTDVLALTGASAALHISDIPFGGPIAGVRVARVGGQLVANPTLAQRAEADLDVVMAA
CCCCCCEEEEECCCEEEEEECCCCCCCHHCEEHHHCCCEEECCCHHHHHHCCCCEEEEEC
SRDAIVMVEGGAQEVSEAVMIEALLFGQAAVQPLLDAQDALRAATGNKARRAFEPPKNDV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCH
ELRAKVKALTWEKVKEAYGRNEKHDRYGRLSEIKKELLQALKDEAAGDAAKLATIALREK
HHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EIKGYYEDVKYDYMRKMITDERRRIGGRGMADIRKITCEVGLLPRVHGSSLFTRGETQAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCEE
VATTLGTAEDEQRVEMLTGMVFKKFMLHYNFPPFSVGEVKFLRSPGRREIGHGALAERAL
EEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHH
RAVMPPEDQFPYTVRVVSDIMESNGSSSMASVCGGCLSLMDAGVPIKAPVAGIAMGLIKE
HHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
GEKIAILSDILGDEDHLGDMDFKVCGTAAGITSIQMDIKIGGVTRDILEQALAQAAEGRK
CCEEEEHHHHCCCCCCCCCCCCEECCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCHH
HILGEMAKALSAPRGSISAYAPRITTIKIRPERIKDIIGPGGKTIKDITARTGTSINIED
HHHHHHHHHHCCCCCCCEECCCEEEEEEECHHHHHHHHCCCCCHHHHHHHCCCCEEEECC
DGSVSIASPNQDKVEEAIKMIRGLTQEAEVGRIYLGTVRKIAEFGAFVEIFPGTDGLIHI
CCCEEECCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCEEEEECCCCCEEEE
SELSDKRVKSVSDVLSEGEEVMVKVISVDRSGKIRLSRKEALADSAKKSEGTEPPKGEPA
HHHCHHHHHHHHHHHHCCHHEEEEEEEECCCCCEEECHHHHHHHHHHHCCCCCCCCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA