Click here to switch to the map view.

The map label for this gene is lysS

Identifier: 220916312

GI number: 220916312

Start: 1354123

End: 1355664

Strand: Direct

Name: lysS

Synonym: A2cp1_1206

Alternate gene names: 220916312

Gene position: 1354123-1355664 (Clockwise)

Preceding gene: 220916311

Following gene: 220916313

Centisome position: 26.92

GC content: 71.98

Gene sequence:

>1542_bases
ATGGCCGACGAACTTGGAACGACCGAGCGCGAGATCATCGCGCAGCGTCTGAAGAAGGCCGAGGCGCTCCGCGCCCTCGG
CGTGAACCCCTTCGGAAACGGCTGGCAGCCCCGCCACCTCGCCGACGAGCTGCTGCGCCACTACGGCGACCAGCCCGCCG
AGGAGATCGCGAAGGACCCCGGCGACTGGTCGCTCGCCGGTCGCGTCCTGGCGGTCCGCTCCTTCGGCAAGGCCGCGTTC
CTGCGCGTCCGCGACCGCTCCGCCGAGCTCCAGGTGTGGGTGAAGAAGGATCGCGTGGGCGAGCAGGCGTTCGAGGTCTT
CAAGCTGCTCGACATCGGCGACATCGTGGGCGCGGAGGGGCCGGCGACCCGCACCAAGACCGGCGAGCTGACGCTGGAGG
CGCGGACGTTCACCATCCTCACCAAGGCGACGCGCCCGCTGCCGGAGAAGTGGCACGGCCTCACCGACGTGGAGCAGCGC
TACCGCCAGCGCTACGTGGACCTGGTGGTGACGCCCGGCGTGCGCGAGGCGTTCGTGAAGCGCGCCCGCATCGTCTCCGG
GATCCGCCGCTTCCTGGACGCGCGCGGCTACCTCGAGGTGGAGACGCCCACGCTGCACAAGCCGGAGGAGGCCGGCGGCG
CGGCCGCGCGGCCGTTCGAGACGCACCACAACGCGCTCGACCTGGACCTGAAGCTCCGCATCGCCACCGAGCTGCACCTG
AAGCGCCTCGTGGTGGGCGGCCTGGATCGCGTGTACGAGATCGGCCGGATCTGGCGCAACGAGGGGATCGACCGGCGCCA
CAACCCGGAGTTCACCTCGATCGAGTTCTACCAGGCGTACGCCACCCACGAGGACCTGATGCGCCTCACCGAGGAGCTGA
TGCACCGGCTCGCGGTGGAGGTGACCGGCGGGCCGGTGGTGACGTTCCAGGGCCAGGCCATCGACCTGACCCCGCCGTTC
CCGCGCGTCTCCATGCTGGAGGTGGGCGCGCGAGCGCTGGGGCTGTCGCCGGACGACGCGCTGGCCGGCCGCGGCCTCGC
CGAGGCGCTCTCGCGCGCGGCGGCGCGCGAGAACGACTCCGAGGACGCCTGGAAGCTGGAGCAGGCCGCGAAGAAGACGC
CGGGCGAGGCGGTGGCGCTCGCGTTCGAGATCTTCGGCGAGCCGCAGCTCCCGAAGGACCGGCCCGCGTTCGTGGTGGAC
TTCCCGCTCGAGACGAGCCCGCTCTCCCGCCGGCGCGACGCCGACCCCCGGCTGGTGGACCGCTTCGAGCTGTTCGCCGC
GGGCATGGAGCTCGCGAACGCGTTCTCCGAGCTGAACGACCCGGCCGACCAGCGCGCGCGGTTCGAGGCGCAGATGCGCG
CCAAGGCCGCCGGCGACGAGGAGGCCATGCCCTACGACGAGGACTTCGTCCGCGCGCTGGAGCACGGCATGCCGCCCACC
GCGGGCGAGGGGATCGGCATCGATCGCCTGGCCATGCTCTTCACCGACTCGGCGTCCATCCGCGACGTGATCCTGTTCCC
GCTGCTGAAGTCCCGCGACTGA

Upstream 100 bases:

>100_bases
GCGGATCCCGCGTTGACGCCCCGCGCCGACCCGACTAGCCTCCCCCCCCGCACGCATCTCCCCGGAGATTCCTCCGAGGA
TCCGAGAGATTCCGCACCCG

Downstream 100 bases:

>100_bases
CCTTGTCCGATCCCGTCCCGCACCGGCCCGGCTCCGAGCCCGGCCCCCGCCCGGCGGCCGCGCCCGGCGCCGCCTCCCGC
GACGGCGCCGCCCACCCGGT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MADELGTTEREIIAQRLKKAEALRALGVNPFGNGWQPRHLADELLRHYGDQPAEEIAKDPGDWSLAGRVLAVRSFGKAAF
LRVRDRSAELQVWVKKDRVGEQAFEVFKLLDIGDIVGAEGPATRTKTGELTLEARTFTILTKATRPLPEKWHGLTDVEQR
YRQRYVDLVVTPGVREAFVKRARIVSGIRRFLDARGYLEVETPTLHKPEEAGGAAARPFETHHNALDLDLKLRIATELHL
KRLVVGGLDRVYEIGRIWRNEGIDRRHNPEFTSIEFYQAYATHEDLMRLTEELMHRLAVEVTGGPVVTFQGQAIDLTPPF
PRVSMLEVGARALGLSPDDALAGRGLAEALSRAAARENDSEDAWKLEQAAKKTPGEAVALAFEIFGEPQLPKDRPAFVVD
FPLETSPLSRRRDADPRLVDRFELFAAGMELANAFSELNDPADQRARFEAQMRAKAAGDEEAMPYDEDFVRALEHGMPPT
AGEGIGIDRLAMLFTDSASIRDVILFPLLKSRD

Sequences:

>Translated_513_residues
MADELGTTEREIIAQRLKKAEALRALGVNPFGNGWQPRHLADELLRHYGDQPAEEIAKDPGDWSLAGRVLAVRSFGKAAF
LRVRDRSAELQVWVKKDRVGEQAFEVFKLLDIGDIVGAEGPATRTKTGELTLEARTFTILTKATRPLPEKWHGLTDVEQR
YRQRYVDLVVTPGVREAFVKRARIVSGIRRFLDARGYLEVETPTLHKPEEAGGAAARPFETHHNALDLDLKLRIATELHL
KRLVVGGLDRVYEIGRIWRNEGIDRRHNPEFTSIEFYQAYATHEDLMRLTEELMHRLAVEVTGGPVVTFQGQAIDLTPPF
PRVSMLEVGARALGLSPDDALAGRGLAEALSRAAARENDSEDAWKLEQAAKKTPGEAVALAFEIFGEPQLPKDRPAFVVD
FPLETSPLSRRRDADPRLVDRFELFAAGMELANAFSELNDPADQRARFEAQMRAKAAGDEEAMPYDEDFVRALEHGMPPT
AGEGIGIDRLAMLFTDSASIRDVILFPLLKSRD
>Mature_512_residues
ADELGTTEREIIAQRLKKAEALRALGVNPFGNGWQPRHLADELLRHYGDQPAEEIAKDPGDWSLAGRVLAVRSFGKAAFL
RVRDRSAELQVWVKKDRVGEQAFEVFKLLDIGDIVGAEGPATRTKTGELTLEARTFTILTKATRPLPEKWHGLTDVEQRY
RQRYVDLVVTPGVREAFVKRARIVSGIRRFLDARGYLEVETPTLHKPEEAGGAAARPFETHHNALDLDLKLRIATELHLK
RLVVGGLDRVYEIGRIWRNEGIDRRHNPEFTSIEFYQAYATHEDLMRLTEELMHRLAVEVTGGPVVTFQGQAIDLTPPFP
RVSMLEVGARALGLSPDDALAGRGLAEALSRAAARENDSEDAWKLEQAAKKTPGEAVALAFEIFGEPQLPKDRPAFVVDF
PLETSPLSRRRDADPRLVDRFELFAAGMELANAFSELNDPADQRARFEAQMRAKAAGDEEAMPYDEDFVRALEHGMPPTA
GEGIGIDRLAMLFTDSASIRDVILFPLLKSRD

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=517, Percent_Identity=41.3926499032882, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI194272210, Length=517, Percent_Identity=41.3926499032882, Blast_Score=358, Evalue=4e-99,
Organism=Escherichia coli, GI1790571, Length=501, Percent_Identity=46.7065868263473, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1789256, Length=503, Percent_Identity=46.1232604373757, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI87082379, Length=339, Percent_Identity=33.3333333333333, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1788173, Length=259, Percent_Identity=28.957528957529, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=518, Percent_Identity=40.3474903474903, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17535927, Length=518, Percent_Identity=40.3474903474903, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71994340, Length=512, Percent_Identity=40.625, Blast_Score=360, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI32566633, Length=202, Percent_Identity=28.2178217821782, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=465, Percent_Identity=42.5806451612903, Blast_Score=336, Evalue=5e-93,
Organism=Saccharomyces cerevisiae, GI6324256, Length=508, Percent_Identity=30.7086614173228, Blast_Score=186, Evalue=9e-48,
Organism=Drosophila melanogaster, GI24640849, Length=517, Percent_Identity=41.0058027079304, Blast_Score=354, Evalue=8e-98,
Organism=Drosophila melanogaster, GI24640851, Length=517, Percent_Identity=41.0058027079304, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24584738, Length=228, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_ANAD2 (B8JFW2)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002491616.1
- ProteinModelPortal:   B8JFW2
- SMR:   B8JFW2
- GeneID:   7299072
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_1206
- HOGENOM:   HBG631383
- ProtClustDB:   PRK00484
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 57296; Mature: 57165

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADELGTTEREIIAQRLKKAEALRALGVNPFGNGWQPRHLADELLRHYGDQPAEEIAKDP
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCC
GDWSLAGRVLAVRSFGKAAFLRVRDRSAELQVWVKKDRVGEQAFEVFKLLDIGDIVGAEG
CCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEHHHCHHHHHHHHHHHHHHHHHCCCC
PATRTKTGELTLEARTFTILTKATRPLPEKWHGLTDVEQRYRQRYVDLVVTPGVREAFVK
CCCCCCCCCEEEEEEEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHEEECCCHHHHHHH
RARIVSGIRRFLDARGYLEVETPTLHKPEEAGGAAARPFETHHNALDLDLKLRIATELHL
HHHHHHHHHHHHCCCCEEEECCCCCCCCHHCCCCCCCCCHHCCCEEEEEEEEEEHHHHHH
KRLVVGGLDRVYEIGRIWRNEGIDRRHNPEFTSIEFYQAYATHEDLMRLTEELMHRLAVE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHEEE
VTGGPVVTFQGQAIDLTPPFPRVSMLEVGARALGLSPDDALAGRGLAEALSRAAARENDS
ECCCEEEEECCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHCCCCC
EDAWKLEQAAKKTPGEAVALAFEIFGEPQLPKDRPAFVVDFPLETSPLSRRRDADPRLVD
HHHHHHHHHHHCCCCHHEEEHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHH
RFELFAAGMELANAFSELNDPADQRARFEAQMRAKAAGDEEAMPYDEDFVRALEHGMPPT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCC
AGEGIGIDRLAMLFTDSASIRDVILFPLLKSRD
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADELGTTEREIIAQRLKKAEALRALGVNPFGNGWQPRHLADELLRHYGDQPAEEIAKDP
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCC
GDWSLAGRVLAVRSFGKAAFLRVRDRSAELQVWVKKDRVGEQAFEVFKLLDIGDIVGAEG
CCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEHHHCHHHHHHHHHHHHHHHHHCCCC
PATRTKTGELTLEARTFTILTKATRPLPEKWHGLTDVEQRYRQRYVDLVVTPGVREAFVK
CCCCCCCCCEEEEEEEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHEEECCCHHHHHHH
RARIVSGIRRFLDARGYLEVETPTLHKPEEAGGAAARPFETHHNALDLDLKLRIATELHL
HHHHHHHHHHHHCCCCEEEECCCCCCCCHHCCCCCCCCCHHCCCEEEEEEEEEEHHHHHH
KRLVVGGLDRVYEIGRIWRNEGIDRRHNPEFTSIEFYQAYATHEDLMRLTEELMHRLAVE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHEEE
VTGGPVVTFQGQAIDLTPPFPRVSMLEVGARALGLSPDDALAGRGLAEALSRAAARENDS
ECCCEEEEECCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHCCCCC
EDAWKLEQAAKKTPGEAVALAFEIFGEPQLPKDRPAFVVDFPLETSPLSRRRDADPRLVD
HHHHHHHHHHHCCCCHHEEEHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHH
RFELFAAGMELANAFSELNDPADQRARFEAQMRAKAAGDEEAMPYDEDFVRALEHGMPPT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCC
AGEGIGIDRLAMLFTDSASIRDVILFPLLKSRD
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA