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The map label for this gene is rfbE [H]

Identifier: 220916296

GI number: 220916296

Start: 1332701

End: 1333759

Strand: Direct

Name: rfbE [H]

Synonym: A2cp1_1189

Alternate gene names: 220916296

Gene position: 1332701-1333759 (Clockwise)

Preceding gene: 220916295

Following gene: 220916297

Centisome position: 26.5

GC content: 74.6

Gene sequence:

>1059_bases
GTGATCGCCGAGCGCGTGCTGGTGGTGGGCGGGGCGGGGTTCATCGGCGCGAACCTCGCGCACCGGCTGCTCTCGTCGGG
GACGCGGGTGCGGCTCCTCGACAGCCTGGCGCGGTCCGGCGTGGAGCGGAACCTGGCCTGGCTGCGCGCCACGCACGGCG
ATCGGCTCGAGGTGATCACGGGCGACGTGCGCGACCCGGGCGCGGTGGCGCGCGCCGTCGAGGGCGCCTCGGCGGTGTTC
CACCTGGCGGCGCAGGTGGCGGTGACCACCTCGCTGGTGGACCCGGTCCAGGACTTCGAGGTGAACGCGCGCGGCACGCT
CAACGTGCTGGAGGCGGTGCGCCGCCAGGACCCGCCGCCGTTCGTGCTCTTCACGTCCACCAACAAGGTCTACGGCGCGC
TCGAGGACGTCCTCCTCGAGGACGCCGGCCGGCGGTACGCTCCGGTCGATCCGGGGCTGCACCGCGGGATCTCCGAGGCG
CGCAACCTCGCCTTCCACTCGCCGTACGGCTGCTCCAAGGGCGCCGCCGAGCAGTACGTCCTCGACTGGGCGCGCACCTA
CGGCGTGCCCGCCACCGTGTTCCGGATGAGCTGCATCTACGGGCCGCACCAGTTCGGCACCGAGGACCAGGGCTGGGTGG
CGCACTTCCTCATCCAGGCCATGGCCCGGCGCCCCATCACGCTCTACGGCGACGGCAAGCAGGTGCGCGACATCCTGTTC
GTGGAGGACCTGCTCGACGCGTTCCTGGCGGCGCGCGAGCGCCCGCGCGAGGTGGCCGGGCGCGCCTTCAACCTCGGGGG
CGGGCCGGACAACACCGTGAGCCTGATCGAGCTGCTCGAGCTCATCGAGGCGCTGGAGGGCGCGCGCCCACGCGTGACGC
TCGAGCCGTGGCGCCTCGCCGACCAGCGCTGGTACGTGTCCGACCCGTCCACCTTCGCGGTGCTGGCCGGGTGGGCGCCG
CGCACCGGCGTGCAGGAGGGCGTGCGGCGGCTGCACGCGTGGCTGGCGGCGGCGGCGCGGCCGCAGCGCCCGGCCACGCT
CGAGGAGGCGGCGACCTAG

Upstream 100 bases:

>100_bases
ACGACGGGCTGGCGGAGCTGGCGGGCTGGCTGGAGGGCCAGATCGCGCACGACCGGGTGGGAGAGGCGAGCGCGGAGCTG
GCCGCGCGGGGGCTGTCGCT

Downstream 100 bases:

>100_bases
ATGGCGGCGCACATGCGCGCTGCGGTGCTGGCGGGCCCGCGGCGGATCGAGCTCGCCGAGGTGGAGCGGCCCCGGCCCGG
GCCGGGCGAGGTGCTGGTGC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: CDP-tyvelose 2-epimerase [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MIAERVLVVGGAGFIGANLAHRLLSSGTRVRLLDSLARSGVERNLAWLRATHGDRLEVITGDVRDPGAVARAVEGASAVF
HLAAQVAVTTSLVDPVQDFEVNARGTLNVLEAVRRQDPPPFVLFTSTNKVYGALEDVLLEDAGRRYAPVDPGLHRGISEA
RNLAFHSPYGCSKGAAEQYVLDWARTYGVPATVFRMSCIYGPHQFGTEDQGWVAHFLIQAMARRPITLYGDGKQVRDILF
VEDLLDAFLAARERPREVAGRAFNLGGGPDNTVSLIELLELIEALEGARPRVTLEPWRLADQRWYVSDPSTFAVLAGWAP
RTGVQEGVRRLHAWLAAAARPQRPATLEEAAT

Sequences:

>Translated_352_residues
MIAERVLVVGGAGFIGANLAHRLLSSGTRVRLLDSLARSGVERNLAWLRATHGDRLEVITGDVRDPGAVARAVEGASAVF
HLAAQVAVTTSLVDPVQDFEVNARGTLNVLEAVRRQDPPPFVLFTSTNKVYGALEDVLLEDAGRRYAPVDPGLHRGISEA
RNLAFHSPYGCSKGAAEQYVLDWARTYGVPATVFRMSCIYGPHQFGTEDQGWVAHFLIQAMARRPITLYGDGKQVRDILF
VEDLLDAFLAARERPREVAGRAFNLGGGPDNTVSLIELLELIEALEGARPRVTLEPWRLADQRWYVSDPSTFAVLAGWAP
RTGVQEGVRRLHAWLAAAARPQRPATLEEAAT
>Mature_352_residues
MIAERVLVVGGAGFIGANLAHRLLSSGTRVRLLDSLARSGVERNLAWLRATHGDRLEVITGDVRDPGAVARAVEGASAVF
HLAAQVAVTTSLVDPVQDFEVNARGTLNVLEAVRRQDPPPFVLFTSTNKVYGALEDVLLEDAGRRYAPVDPGLHRGISEA
RNLAFHSPYGCSKGAAEQYVLDWARTYGVPATVFRMSCIYGPHQFGTEDQGWVAHFLIQAMARRPITLYGDGKQVRDILF
VEDLLDAFLAARERPREVAGRAFNLGGGPDNTVSLIELLELIEALEGARPRVTLEPWRLADQRWYVSDPSTFAVLAGWAP
RTGVQEGVRRLHAWLAAAARPQRPATLEEAAT

Specific function: Catalyzes the isomeration of CDP-paratose to CDP- tyvelose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=345, Percent_Identity=26.3768115942029, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI42516563, Length=329, Percent_Identity=26.1398176291793, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI56237023, Length=356, Percent_Identity=26.4044943820225, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI56118217, Length=356, Percent_Identity=26.4044943820225, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI189083684, Length=356, Percent_Identity=26.4044943820225, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI239745448, Length=296, Percent_Identity=25.3378378378378, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI310132178, Length=296, Percent_Identity=25, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI310113012, Length=296, Percent_Identity=25, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788353, Length=362, Percent_Identity=28.7292817679558, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI48994969, Length=354, Percent_Identity=28.2485875706215, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786974, Length=325, Percent_Identity=26.7692307692308, Blast_Score=81, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=335, Percent_Identity=23.8805970149254, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115532424, Length=337, Percent_Identity=25.5192878338279, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71982035, Length=357, Percent_Identity=26.3305322128852, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=365, Percent_Identity=26.5753424657534, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=347, Percent_Identity=25.0720461095101, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=26.1127596439169, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.10 [H]

Molecular weight: Translated: 38418; Mature: 38418

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAERVLVVGGAGFIGANLAHRLLSSGTRVRLLDSLARSGVERNLAWLRATHGDRLEVIT
CCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEE
GDVRDPGAVARAVEGASAVFHLAAQVAVTTSLVDPVQDFEVNARGTLNVLEAVRRQDPPP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHCCCCCC
FVLFTSTNKVYGALEDVLLEDAGRRYAPVDPGLHRGISEARNLAFHSPYGCSKGAAEQYV
EEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
LDWARTYGVPATVFRMSCIYGPHQFGTEDQGWVAHFLIQAMARRPITLYGDGKQVRDILF
HHHHHHCCCCHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHH
VEDLLDAFLAARERPREVAGRAFNLGGGPDNTVSLIELLELIEALEGARPRVTLEPWRLA
HHHHHHHHHHHHCCCHHHHCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
DQRWYVSDPSTFAVLAGWAPRTGVQEGVRRLHAWLAAAARPQRPATLEEAAT
CCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure
MIAERVLVVGGAGFIGANLAHRLLSSGTRVRLLDSLARSGVERNLAWLRATHGDRLEVIT
CCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEE
GDVRDPGAVARAVEGASAVFHLAAQVAVTTSLVDPVQDFEVNARGTLNVLEAVRRQDPPP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHCCCCCC
FVLFTSTNKVYGALEDVLLEDAGRRYAPVDPGLHRGISEARNLAFHSPYGCSKGAAEQYV
EEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
LDWARTYGVPATVFRMSCIYGPHQFGTEDQGWVAHFLIQAMARRPITLYGDGKQVRDILF
HHHHHHCCCCHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHH
VEDLLDAFLAARERPREVAGRAFNLGGGPDNTVSLIELLELIEALEGARPRVTLEPWRLA
HHHHHHHHHHHHCCCHHHHCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
DQRWYVSDPSTFAVLAGWAPRTGVQEGVRRLHAWLAAAARPQRPATLEEAAT
CCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504; 12642575 [H]