Click here to switch to the map view.

The map label for this gene is ruvC

Identifier: 220916122

GI number: 220916122

Start: 1136113

End: 1136670

Strand: Direct

Name: ruvC

Synonym: A2cp1_1009

Alternate gene names: 220916122

Gene position: 1136113-1136670 (Clockwise)

Preceding gene: 220916121

Following gene: 220916123

Centisome position: 22.59

GC content: 80.29

Gene sequence:

>558_bases
GTGATCGTGCTCGGCATCGACCCCGGCTCCCGCCGCTGCGGCTACGGGGTCGTCGCGCGGGAGGGGGCCAGGCTGACGGT
GGTCGAGTCCGGGGTGCTCGTCCCGGGCGACCTGCCCATGGCCCAGCGGCTGGGGCGCATCCTCGACGGCCTCGACGCGC
TCATCGCCCGGGCCCGCCCGCTGGAGGCGTCGGTCGAGTCGGTCTTCTCCGGCGCCTCGCCGCGCTCGGCGCTGGTGCTC
GGGCAGGCGCGCGGCGTCGCGCTCGCCGCCGCCGCGCGCGCCGGGCTCCCCGTCTTCGAGTACGCGCCGTCCGAGGTGAA
GCTCGCCTTCACCGGCAACGGGCGCGCCGGCAAGGACCAGATGCTCCGCACCGCCCGCATGCTCCTCGGCGCGGCGCCCG
GCCTCTCCGACGAGGCCGACGCGCTCGCCATCGCCGTGTGCCACCTCGCCCGGCGCGCCTTCGCGGTGCCGGCGGCCGGC
GCCGGCCGCGCCGCCGCGGCGCGCGCGGCCGCGGCCCGGCTCCGCCCCTCGCGCCGCGACCACCGGGGGACCCCGTGA

Upstream 100 bases:

>100_bases
CACCCGACGATGTTCCCGCCCGCCGCGAGCTGAGCCGGGCGGTCGCGCGACCCGCGCGCCGGGCGCTGGACGCCGGTTCA
GGCTGTGCTACGGTGCCCGC

Downstream 100 bases:

>100_bases
TCGCGCGCCTGGCCGGGCGGATCGCCGAGAAGCGCGACGACCACGCCATCGTGGACGTGGGCGGGGTCGGCTACCTCGTG
CACCTCTCGCAGCTCTCGCT

Product: crossover junction endodeoxyribonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MIVLGIDPGSRRCGYGVVAREGARLTVVESGVLVPGDLPMAQRLGRILDGLDALIARARPLEASVESVFSGASPRSALVL
GQARGVALAAAARAGLPVFEYAPSEVKLAFTGNGRAGKDQMLRTARMLLGAAPGLSDEADALAIAVCHLARRAFAVPAAG
AGRAAAARAAAARLRPSRRDHRGTP

Sequences:

>Translated_185_residues
MIVLGIDPGSRRCGYGVVAREGARLTVVESGVLVPGDLPMAQRLGRILDGLDALIARARPLEASVESVFSGASPRSALVL
GQARGVALAAAARAGLPVFEYAPSEVKLAFTGNGRAGKDQMLRTARMLLGAAPGLSDEADALAIAVCHLARRAFAVPAAG
AGRAAAARAAAARLRPSRRDHRGTP
>Mature_185_residues
MIVLGIDPGSRRCGYGVVAREGARLTVVESGVLVPGDLPMAQRLGRILDGLDALIARARPLEASVESVFSGASPRSALVL
GQARGVALAAAARAGLPVFEYAPSEVKLAFTGNGRAGKDQMLRTARMLLGAAPGLSDEADALAIAVCHLARRAFAVPAAG
AGRAAAARAAAARLRPSRRDHRGTP

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=130, Percent_Identity=45.3846153846154, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_ANAD2 (B8JF03)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002491426.1
- ProteinModelPortal:   B8JF03
- SMR:   B8JF03
- GeneID:   7297055
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_1009
- HOGENOM:   HBG437552
- ProtClustDB:   CLSK945365
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18924; Mature: 18924

Theoretical pI: Translated: 11.72; Mature: 11.72

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVLGIDPGSRRCGYGVVAREGARLTVVESGVLVPGDLPMAQRLGRILDGLDALIARARP
CEEEECCCCCCCCCCCEEECCCCEEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHCCCC
LEASVESVFSGASPRSALVLGQARGVALAAAARAGLPVFEYAPSEVKLAFTGNGRAGKDQ
CHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEECCCEEEEEEECCCCCCHHH
MLRTARMLLGAAPGLSDEADALAIAVCHLARRAFAVPAAGAGRAAAARAAAARLRPSRRD
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
HRGTP
CCCCC
>Mature Secondary Structure
MIVLGIDPGSRRCGYGVVAREGARLTVVESGVLVPGDLPMAQRLGRILDGLDALIARARP
CEEEECCCCCCCCCCCEEECCCCEEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHCCCC
LEASVESVFSGASPRSALVLGQARGVALAAAARAGLPVFEYAPSEVKLAFTGNGRAGKDQ
CHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEECCCEEEEEEECCCCCCHHH
MLRTARMLLGAAPGLSDEADALAIAVCHLARRAFAVPAAGAGRAAAARAAAARLRPSRRD
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
HRGTP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA