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The map label for this gene is mdtK [H]

Identifier: 220916119

GI number: 220916119

Start: 1131147

End: 1132556

Strand: Reverse

Name: mdtK [H]

Synonym: A2cp1_1006

Alternate gene names: 220916119

Gene position: 1132556-1131147 (Counterclockwise)

Preceding gene: 220916125

Following gene: 220916118

Centisome position: 22.52

GC content: 77.02

Gene sequence:

>1410_bases
ATGTTGCGCGCCCCCATGTCCCCGCCGCCGCACGTCCTTCGCACGGAGCTCCGCGCGCTCCTCCGGCTCGCCCTGCCGCT
CTCGCTCGCGCACGGCGGCCAGGCGCTGATGGGCGTGGTGGACGCCGCCATCGTGGGACGCGCGGGCGCGGTGCCGCTCG
CCGGCACCGGCCTCGGCGCCTCGCTGTTCTTCGCGGTGGCGGTGCTCGGCATGGGGATCATGCACGGGCTCGACCCGCTC
ATCTCGCAGGCCATCGGCGCGGGCGACGAGCGGCGCGCCCGGCGCATGCTGTGGCAGGCGGCGTGGCTGGCGGCGTTCCT
CGCCGCCGCGTTCACCATCCCCATCGCGCTCCTCCCGGCGGTGCTGGTGCCGCTCGGCATCTCGCCCGACGTCGCGGCGC
AGGCGGCGCCGTACATCTGGTGGCGGCTCCCCGGCCTGTTCTTCTTCCTCTATTACTTCGGCGCGCGCGCCTACCTGCAG
GCGCACGGCCTGGGCCGGCCCATGGTCGTGGCGACGGTGGGCGCGAACCTCCTGAACGTGCCCGCCGACCTGCTGCTCGT
GTTCGGCGGCGCGTCGCTGCCGGCGTGGACCGGGCCGCTCCGGCTGGTGCCGGCGCTGGGCGCGGCCGGCGCCGCCATCG
CCACCAGCCTGTGCATCGCGCTCCAGGCCCTGGTCCTCGTGCGCGCCACGCGCGCCGTGCCGCTCGCGGGTCCGCGGCCG
GCGGGGCTGGCGCGGCCGGACCGCGCCGCGCTCGGGACCGCGGTGCGGGTGGGCGGGCCGGTCGGGCTGCACATGTTCGC
CGAGGTGGGCTTCTTCTCGCTGGTGAGCTTCCTCGCCGGCCGGCTCGGCGCCGAGGCGCTCGGGGCGCACCAGATCGCGC
TGTCGGTGGCGAGCCTCACGTTCACCATGGCGGTGGGCCTCGGCGACGCGGGCAGCGTGCGGGTGGGGCTCGCGGTGGGC
GCGCACGATCGCGCCGGCGCCCGCCGCGCCGGGCTGTGCGCGTTCGCGGGCGGGGCGTCGATCATGCTCGTCCCCGCGCT
CGCCTTCACCTTCGCGCCCGGCGTCATCGGCCGGCTCATGACCGACGATCCGGGCGTGCTCGCGCTGGCGGTCCCGCTGC
TGCGCGTGGCCGCGATCTTCCAGCTCTCCGACGGGCTCCAGGCGGTCGGGGCCGGCGTGCTGCGCGGCGCGGGCGAGACC
CGCTTCACGTTCGCCGCGAACCTCGCCGGCCACTGGCTGATCGGGCTGCCCGCGACGCTGCTGCTCGGCTTCACCCTCGG
TCACGGGGTGGTCGGGCTGTGGGGCGGCTTCTGCTTCGGCCTCACCGCGGTCGCGATCGCGCTCCTCGGCAGGTTCCTGC
GGGTCTCCGCGCGCGGCATCGTGCCGCTCGCCGAGCGCGCCGCCGCGTAG

Upstream 100 bases:

>100_bases
CCCGCCCGAACGGGGCACCTCGCGGAGCCGCGGCCGTGCGCCCGGAGACCGGCACGGCGGGCCGGCGGCGGCGCCCGTGC
AGCCCTCCCGCACCTCGGGT

Downstream 100 bases:

>100_bases
CGGCGCCCCGGCGTCCCGCCCGCCCCGGCCGGCGCGCGTTGCTATCCTGCGGGGCGATGCGGCTCGCGCTGCCCCAGCCC
TACGAGGACCTCGGCCACGT

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MLRAPMSPPPHVLRTELRALLRLALPLSLAHGGQALMGVVDAAIVGRAGAVPLAGTGLGASLFFAVAVLGMGIMHGLDPL
ISQAIGAGDERRARRMLWQAAWLAAFLAAAFTIPIALLPAVLVPLGISPDVAAQAAPYIWWRLPGLFFFLYYFGARAYLQ
AHGLGRPMVVATVGANLLNVPADLLLVFGGASLPAWTGPLRLVPALGAAGAAIATSLCIALQALVLVRATRAVPLAGPRP
AGLARPDRAALGTAVRVGGPVGLHMFAEVGFFSLVSFLAGRLGAEALGAHQIALSVASLTFTMAVGLGDAGSVRVGLAVG
AHDRAGARRAGLCAFAGGASIMLVPALAFTFAPGVIGRLMTDDPGVLALAVPLLRVAAIFQLSDGLQAVGAGVLRGAGET
RFTFAANLAGHWLIGLPATLLLGFTLGHGVVGLWGGFCFGLTAVAIALLGRFLRVSARGIVPLAERAAA

Sequences:

>Translated_469_residues
MLRAPMSPPPHVLRTELRALLRLALPLSLAHGGQALMGVVDAAIVGRAGAVPLAGTGLGASLFFAVAVLGMGIMHGLDPL
ISQAIGAGDERRARRMLWQAAWLAAFLAAAFTIPIALLPAVLVPLGISPDVAAQAAPYIWWRLPGLFFFLYYFGARAYLQ
AHGLGRPMVVATVGANLLNVPADLLLVFGGASLPAWTGPLRLVPALGAAGAAIATSLCIALQALVLVRATRAVPLAGPRP
AGLARPDRAALGTAVRVGGPVGLHMFAEVGFFSLVSFLAGRLGAEALGAHQIALSVASLTFTMAVGLGDAGSVRVGLAVG
AHDRAGARRAGLCAFAGGASIMLVPALAFTFAPGVIGRLMTDDPGVLALAVPLLRVAAIFQLSDGLQAVGAGVLRGAGET
RFTFAANLAGHWLIGLPATLLLGFTLGHGVVGLWGGFCFGLTAVAIALLGRFLRVSARGIVPLAERAAA
>Mature_469_residues
MLRAPMSPPPHVLRTELRALLRLALPLSLAHGGQALMGVVDAAIVGRAGAVPLAGTGLGASLFFAVAVLGMGIMHGLDPL
ISQAIGAGDERRARRMLWQAAWLAAFLAAAFTIPIALLPAVLVPLGISPDVAAQAAPYIWWRLPGLFFFLYYFGARAYLQ
AHGLGRPMVVATVGANLLNVPADLLLVFGGASLPAWTGPLRLVPALGAAGAAIATSLCIALQALVLVRATRAVPLAGPRP
AGLARPDRAALGTAVRVGGPVGLHMFAEVGFFSLVSFLAGRLGAEALGAHQIALSVASLTFTMAVGLGDAGSVRVGLAVG
AHDRAGARRAGLCAFAGGASIMLVPALAFTFAPGVIGRLMTDDPGVLALAVPLLRVAAIFQLSDGLQAVGAGVLRGAGET
RFTFAANLAGHWLIGLPATLLLGFTLGHGVVGLWGGFCFGLTAVAIALLGRFLRVSARGIVPLAERAAA

Specific function: Multidrug efflux pump that functions probably as a Na(+)/drug antiporter [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MdtK subfamily [H]

Homologues:

Organism=Homo sapiens, GI153792564, Length=424, Percent_Identity=27.5943396226415, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI22907060, Length=462, Percent_Identity=26.8398268398268, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI38261960, Length=460, Percent_Identity=26.0869565217391, Blast_Score=109, Evalue=6e-24,
Organism=Escherichia coli, GI48994890, Length=455, Percent_Identity=30.7692307692308, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR022913 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 47647; Mature: 47647

Theoretical pI: Translated: 11.95; Mature: 11.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRAPMSPPPHVLRTELRALLRLALPLSLAHGGQALMGVVDAAIVGRAGAVPLAGTGLGA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHH
SLFFAVAVLGMGIMHGLDPLISQAIGAGDERRARRMLWQAAWLAAFLAAAFTIPIALLPA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLVPLGISPDVAAQAAPYIWWRLPGLFFFLYYFGARAYLQAHGLGRPMVVATVGANLLNV
HHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHC
PADLLLVFGGASLPAWTGPLRLVPALGAAGAAIATSLCIALQALVLVRATRAVPLAGPRP
CHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AGLARPDRAALGTAVRVGGPVGLHMFAEVGFFSLVSFLAGRLGAEALGAHQIALSVASLT
CCCCCCCHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
FTMAVGLGDAGSVRVGLAVGAHDRAGARRAGLCAFAGGASIMLVPALAFTFAPGVIGRLM
HHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
TDDPGVLALAVPLLRVAAIFQLSDGLQAVGAGVLRGAGETRFTFAANLAGHWLIGLPATL
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHH
LLGFTLGHGVVGLWGGFCFGLTAVAIALLGRFLRVSARGIVPLAERAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure
MLRAPMSPPPHVLRTELRALLRLALPLSLAHGGQALMGVVDAAIVGRAGAVPLAGTGLGA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHH
SLFFAVAVLGMGIMHGLDPLISQAIGAGDERRARRMLWQAAWLAAFLAAAFTIPIALLPA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLVPLGISPDVAAQAAPYIWWRLPGLFFFLYYFGARAYLQAHGLGRPMVVATVGANLLNV
HHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHC
PADLLLVFGGASLPAWTGPLRLVPALGAAGAAIATSLCIALQALVLVRATRAVPLAGPRP
CHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AGLARPDRAALGTAVRVGGPVGLHMFAEVGFFSLVSFLAGRLGAEALGAHQIALSVASLT
CCCCCCCHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
FTMAVGLGDAGSVRVGLAVGAHDRAGARRAGLCAFAGGASIMLVPALAFTFAPGVIGRLM
HHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
TDDPGVLALAVPLLRVAAIFQLSDGLQAVGAGVLRGAGETRFTFAANLAGHWLIGLPATL
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHH
LLGFTLGHGVVGLWGGFCFGLTAVAIALLGRFLRVSARGIVPLAERAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA