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The map label for this gene is gltB [H]

Identifier: 220915984

GI number: 220915984

Start: 981933

End: 983420

Strand: Direct

Name: gltB [H]

Synonym: A2cp1_0870

Alternate gene names: 220915984

Gene position: 981933-983420 (Clockwise)

Preceding gene: 220915983

Following gene: 220915992

Centisome position: 19.52

GC content: 72.04

Gene sequence:

>1488_bases
ATGGGCAAGCCGACCGGATTCATGGAGTTCCCGCGCGAGCCGAAGCACGAGCGGCCGCCGCTGGAGCGCATCGCGGACTG
GAGCGAGGCGCACCCCGCCTACCCGGAGCAGACGCTCCGGCAGCAGGGCGCGCGGTGCATGGACTGCGGCATCCCGTTCT
GCCACACCGGCAAGATCATCGCCGGCATGGCGGCCGGGTGCCCGGTGCAGAACCTCATCCCGGAGTGGAACGACCTCGTC
TACCGCGGGCAGTGGGAGGAGGCGTACATCCGCCTCGCCAAGACCAACAACTTCCCCGAGTTCACCGGGCGCGTCTGCCC
GGCGCCGTGCGAGGGGAGCTGCACGCTCGGCATCCACGAGCCGCCCGTCACCATCAAGACCATCGAGGCGGAGATCGTGG
ACCGGGCGTTCGCGGCCGGGCTGGTGCGCCCGCAGCCGCCGCCGGAGCGCACCGGCAAGCGGGTGGCGATCGTCGGCTCC
GGGCCGGCGGGCCTCGCCGCCGCGCAGCAGCTCAACAAGGCCGGGCACGCGGTGACGGTGTTCGAGCGCGCCGACCGGGT
GGGCGGGCTGCTCATGTACGGCATCCCGAACATGAAGCTCGACAAGGCGCTGGTGGAGCGGCGGGTGAAGCTGCTCGCGG
ACGAGGGGGTGCGCTTCGTCACCGAGACCGAGATCGGCCGGCACATCACCGCCGACCGGCTGGTGAAGGACTACGACGCG
GTGGTGCTGGCCGGCGGCGCCACGCACGCGCGCGACCTGCCCATCGAGGGCCGCAGCCTCCAGGGCATCCACCTCGCCAT
GGAGTTCCTGCACGCGAACACGAAGTCGCTGCTCGACTCGAACCACGAGGACGGCCGGTACATCTCGGCGAAGGGCAAGC
ACGTGGTCGTCATCGGCGGCGGCGACACCGGCACCGACTGCGTCGGGACCGCCATCCGGCACGGCGCCCGCAGCGTGACC
CAGCTCGAGATCATGGCGCGCCCGCCCGACCAGCGGCCGCGCGACAACCCCTGGCCGCAGTGGCCCAGGGTCCTCAAGAC
CGACTACGGCCAGGAGGAGGCGAAGGCGGTCTTCGGGCACGACCCGCGCCGGTTCTGCGCGCTCACCAAGCGCTTCATCG
GCGACGCGCAGGGCAAGGTGAAGGAGCTGCACCTGGTGGAGGTGGAGTGGGTGAAGGGCCCCGACGGCCGCTCCGGCCCG
AAGGAGATCCCGCACACCGACCGGATCATCCCCGGCGAGCTGGTGCTGCTCGCGCTCGGCTTCGTCGGCCCCGAGAAGCC
CGTGCTGCAGCAGTTCGGCGTGAAGCTGGACGACCGCGGCAACGTGTGGACGGACGAGCACCGGATGACGAGCGTGCCCG
GCGTGTTCGCCGCGGGCGACATGGCCCGCGGCCAGTCGCTGGTGGTGTGGGCCATCCGCGAGGGGCGCCAGGCGGCGCAG
GCGGTGGACCAGTTCCTCTCGCACGGCGAGACGGTCCTGCCCCCCTGA

Upstream 100 bases:

>100_bases
AGGCGCGGATGCGCGAGAAGGGCCTCACGCCGGAGCAGGCGGAGCTGGCCGCCTTCGAGGAGAACACCCAGGACGCCGCG
CGCGTGAGCGGGAACTAGAC

Downstream 100 bases:

>100_bases
CCCGGAGACGCGAGGTCCCGCCCGCCCCGGGAACGCGCCCGGGGTGGGCGGGGCCACGCTCGCCGGCGGCGGCTACCGCG
TCCCGGTCGATCCGCCCTGC

Product: glutamate synthase, NADH/NADPH, small subunit

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MGKPTGFMEFPREPKHERPPLERIADWSEAHPAYPEQTLRQQGARCMDCGIPFCHTGKIIAGMAAGCPVQNLIPEWNDLV
YRGQWEEAYIRLAKTNNFPEFTGRVCPAPCEGSCTLGIHEPPVTIKTIEAEIVDRAFAAGLVRPQPPPERTGKRVAIVGS
GPAGLAAAQQLNKAGHAVTVFERADRVGGLLMYGIPNMKLDKALVERRVKLLADEGVRFVTETEIGRHITADRLVKDYDA
VVLAGGATHARDLPIEGRSLQGIHLAMEFLHANTKSLLDSNHEDGRYISAKGKHVVVIGGGDTGTDCVGTAIRHGARSVT
QLEIMARPPDQRPRDNPWPQWPRVLKTDYGQEEAKAVFGHDPRRFCALTKRFIGDAQGKVKELHLVEVEWVKGPDGRSGP
KEIPHTDRIIPGELVLLALGFVGPEKPVLQQFGVKLDDRGNVWTDEHRMTSVPGVFAAGDMARGQSLVVWAIREGRQAAQ
AVDQFLSHGETVLPP

Sequences:

>Translated_495_residues
MGKPTGFMEFPREPKHERPPLERIADWSEAHPAYPEQTLRQQGARCMDCGIPFCHTGKIIAGMAAGCPVQNLIPEWNDLV
YRGQWEEAYIRLAKTNNFPEFTGRVCPAPCEGSCTLGIHEPPVTIKTIEAEIVDRAFAAGLVRPQPPPERTGKRVAIVGS
GPAGLAAAQQLNKAGHAVTVFERADRVGGLLMYGIPNMKLDKALVERRVKLLADEGVRFVTETEIGRHITADRLVKDYDA
VVLAGGATHARDLPIEGRSLQGIHLAMEFLHANTKSLLDSNHEDGRYISAKGKHVVVIGGGDTGTDCVGTAIRHGARSVT
QLEIMARPPDQRPRDNPWPQWPRVLKTDYGQEEAKAVFGHDPRRFCALTKRFIGDAQGKVKELHLVEVEWVKGPDGRSGP
KEIPHTDRIIPGELVLLALGFVGPEKPVLQQFGVKLDDRGNVWTDEHRMTSVPGVFAAGDMARGQSLVVWAIREGRQAAQ
AVDQFLSHGETVLPP
>Mature_494_residues
GKPTGFMEFPREPKHERPPLERIADWSEAHPAYPEQTLRQQGARCMDCGIPFCHTGKIIAGMAAGCPVQNLIPEWNDLVY
RGQWEEAYIRLAKTNNFPEFTGRVCPAPCEGSCTLGIHEPPVTIKTIEAEIVDRAFAAGLVRPQPPPERTGKRVAIVGSG
PAGLAAAQQLNKAGHAVTVFERADRVGGLLMYGIPNMKLDKALVERRVKLLADEGVRFVTETEIGRHITADRLVKDYDAV
VLAGGATHARDLPIEGRSLQGIHLAMEFLHANTKSLLDSNHEDGRYISAKGKHVVVIGGGDTGTDCVGTAIRHGARSVTQ
LEIMARPPDQRPRDNPWPQWPRVLKTDYGQEEAKAVFGHDPRRFCALTKRFIGDAQGKVKELHLVEVEWVKGPDGRSGPK
EIPHTDRIIPGELVLLALGFVGPEKPVLQQFGVKLDDRGNVWTDEHRMTSVPGVFAAGDMARGQSLVVWAIREGRQAAQA
VDQFLSHGETVLPP

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=481, Percent_Identity=23.4927234927235, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1789606, Length=489, Percent_Identity=35.7873210633947, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI1788811, Length=474, Percent_Identity=34.5991561181435, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI87082180, Length=469, Percent_Identity=33.4754797441365, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788468, Length=461, Percent_Identity=25.5965292841649, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1789243, Length=432, Percent_Identity=27.5462962962963, Blast_Score=108, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17570289, Length=506, Percent_Identity=49.0118577075099, Blast_Score=494, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI71984108, Length=482, Percent_Identity=23.4439834024896, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17543860, Length=165, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=494, Percent_Identity=51.6194331983806, Blast_Score=528, Evalue=1e-151,
Organism=Drosophila melanogaster, GI28574881, Length=497, Percent_Identity=53.7223340040241, Blast_Score=554, Evalue=1e-158,
Organism=Drosophila melanogaster, GI24665539, Length=497, Percent_Identity=53.7223340040241, Blast_Score=554, Evalue=1e-158,
Organism=Drosophila melanogaster, GI24665547, Length=496, Percent_Identity=53.6290322580645, Blast_Score=553, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24665543, Length=496, Percent_Identity=53.6290322580645, Blast_Score=553, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24640763, Length=328, Percent_Identity=27.7439024390244, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI18858217, Length=328, Percent_Identity=27.7439024390244, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54285; Mature: 54154

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPTGFMEFPREPKHERPPLERIADWSEAHPAYPEQTLRQQGARCMDCGIPFCHTGKII
CCCCCCCCCCCCCCCCCCCCHHHHHCHHHCCCCCCHHHHHHCCCEEECCCCCCEECCCHH
AGMAAGCPVQNLIPEWNDLVYRGQWEEAYIRLAKTNNFPEFTGRVCPAPCEGSCTLGIHE
HHHHCCCCHHHHCCCCHHHEECCCCHHHHEEEECCCCCCHHCCCCCCCCCCCCEEECCCC
PPVTIKTIEAEIVDRAFAAGLVRPQPPPERTGKRVAIVGSGPAGLAAAQQLNKAGHAVTV
CCCEEEEHHHHHHHHHHHHCCCCCCCCCHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEE
FERADRVGGLLMYGIPNMKLDKALVERRVKLLADEGVRFVTETEIGRHITADRLVKDYDA
EHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCHHHHHHCCCE
VVLAGGATHARDLPIEGRSLQGIHLAMEFLHANTKSLLDSNHEDGRYISAKGKHVVVIGG
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEECCCCEEEEEEC
GDTGTDCVGTAIRHGARSVTQLEIMARPPDQRPRDNPWPQWPRVLKTDYGQEEAKAVFGH
CCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHCC
DPRRFCALTKRFIGDAQGKVKELHLVEVEWVKGPDGRSGPKEIPHTDRIIPGELVLLALG
CHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
FVGPEKPVLQQFGVKLDDRGNVWTDEHRMTSVPGVFAAGDMARGQSLVVWAIREGRQAAQ
CCCCCCHHHHHCCCEECCCCCEECCCCCCCCCCCEEECCCCCCCCEEEEEEECCCHHHHH
AVDQFLSHGETVLPP
HHHHHHHCCCCCCCC
>Mature Secondary Structure 
GKPTGFMEFPREPKHERPPLERIADWSEAHPAYPEQTLRQQGARCMDCGIPFCHTGKII
CCCCCCCCCCCCCCCCCCCHHHHHCHHHCCCCCCHHHHHHCCCEEECCCCCCEECCCHH
AGMAAGCPVQNLIPEWNDLVYRGQWEEAYIRLAKTNNFPEFTGRVCPAPCEGSCTLGIHE
HHHHCCCCHHHHCCCCHHHEECCCCHHHHEEEECCCCCCHHCCCCCCCCCCCCEEECCCC
PPVTIKTIEAEIVDRAFAAGLVRPQPPPERTGKRVAIVGSGPAGLAAAQQLNKAGHAVTV
CCCEEEEHHHHHHHHHHHHCCCCCCCCCHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEE
FERADRVGGLLMYGIPNMKLDKALVERRVKLLADEGVRFVTETEIGRHITADRLVKDYDA
EHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCHHHHHHCCCE
VVLAGGATHARDLPIEGRSLQGIHLAMEFLHANTKSLLDSNHEDGRYISAKGKHVVVIGG
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEECCCCEEEEEEC
GDTGTDCVGTAIRHGARSVTQLEIMARPPDQRPRDNPWPQWPRVLKTDYGQEEAKAVFGH
CCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHCC
DPRRFCALTKRFIGDAQGKVKELHLVEVEWVKGPDGRSGPKEIPHTDRIIPGELVLLALG
CHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
FVGPEKPVLQQFGVKLDDRGNVWTDEHRMTSVPGVFAAGDMARGQSLVVWAIREGRQAAQ
CCCCCCHHHHHCCCEECCCCCEECCCCCCCCCCCEEECCCCCCCCEEEEEEECCCHHHHH
AVDQFLSHGETVLPP
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]