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The map label for this gene is mdlA [H]

Identifier: 220915975

GI number: 220915975

Start: 965831

End: 966928

Strand: Direct

Name: mdlA [H]

Synonym: A2cp1_0861

Alternate gene names: 220915975

Gene position: 965831-966928 (Clockwise)

Preceding gene: 220915974

Following gene: 220915976

Centisome position: 19.2

GC content: 79.6

Gene sequence:

>1098_bases
GTGGCGCGCGGCGAGGCCACCGTGGAGCGGGTGCACGCGGCCGCGTACCGCGTGCCCACCGACGCGCCCGAGTCGGACGG
CACGCTGGCCTGGGACGCGACCACGCTGGTGGTGGCCGAGGTGGAGGGCGGCGGCGCGCGCGGGCTCGGCTACACCTACG
CGGACGCGTCCGCCGCCGCGGTGGTGAACGGCCTGCTCTCCGAGGCGGTGCGGGGGGTGGACGCGCTCGACGCGCCCGCC
GCGAGCCGCGCCATGCGCCGGGCGCTGCGCAACGCCGGGCCGGCCGGCGTCGGCGGCATGGCGCTCTCGGCGGTGGACGC
GGCGCTCTGGGACCTCCACGCGCGGCTGCTGGGGGTGCCGCTCCTCGCGCTGCTGGGCCGTGCGCGGCCGGCCGTCCCGG
CCTACGGGAGCGGCGGCTTCTGCTCGTACGCGCCGGAGCGCCTGCGCGAGCAGCTCGGCGGCTGGGCGGCGGCGGGCTTC
CCGGCGGTGAAGATGAAGGTCGGGCGCGACCCGCGCGCCGACCGGGAGCGCGTGCGCGCGGCGCGGGAGGCGATCGGCGC
GGCGGGGCTGTTCGTGGACGCGAACGGCGCCTACGGGCGCAAGCAGGCGCTGGCGCTCGCCGCGGCGTTCGCCGACGAGG
GCGTCACCTGGCTCGAGGAGCCGGTGCCGTCCGACGACCTGGCCGGCCTGCGCCTCGTCCGCGAGCGCGCGCCCGCCGGG
ATGGACGTGGCCGCGGGCGAGTACGGGCACGACCCGGCCTACTTCCGCCGCATGCTCGAGGCGGGCGCCGTGGACGTGCT
GCAGGCGGACGCGACCCGCTGCGGCGGGGTGACCGGCTTCCTGGCCGCGGCGGCGGTGGCGGCGGCGTTCGGTGTCCCGC
TGTCGTCGCACTGCGCGCCCTCGCTCCACGCGACCCTGATGTGCGCGGTGGAGCGGGCCGCCCATGCGGAGTGGTTCCAC
GATCACGAGCGCATCGAGGCGCTGCTGCTCGAGGGCGCGCCGCGCCCGTCCGGTGGCGCGCTCGCGCCGGACCTCTCGCG
CCCCGGCCTCGGCCTCGAGCTCCGCGCGCGCGACGCGGAGCGCTGGGCGGTGGCCTGA

Upstream 100 bases:

>100_bases
CTCGAGGCGATCCTGAAGGGCGACCCGGAGCGCGGCGCCATGGTCGTGCGCTCGTGGCGCGACGCGCTGGAGGGCGTGCT
GCCCCACCGGAAGGGCTGAC

Downstream 100 bases:

>100_bases
GGGGAGGGGCGATGGAGCAGCCGGAGGCGGGCGGGCGGTATCTCATCCCTGGGCTGGGCCGGTCGGCGGATCCGGCCGCG
CGCCTGCGCGCCGAGCGCGA

Product: Mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: MR [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MARGEATVERVHAAAYRVPTDAPESDGTLAWDATTLVVAEVEGGGARGLGYTYADASAAAVVNGLLSEAVRGVDALDAPA
ASRAMRRALRNAGPAGVGGMALSAVDAALWDLHARLLGVPLLALLGRARPAVPAYGSGGFCSYAPERLREQLGGWAAAGF
PAVKMKVGRDPRADRERVRAAREAIGAAGLFVDANGAYGRKQALALAAAFADEGVTWLEEPVPSDDLAGLRLVRERAPAG
MDVAAGEYGHDPAYFRRMLEAGAVDVLQADATRCGGVTGFLAAAAVAAAFGVPLSSHCAPSLHATLMCAVERAAHAEWFH
DHERIEALLLEGAPRPSGGALAPDLSRPGLGLELRARDAERWAVA

Sequences:

>Translated_365_residues
MARGEATVERVHAAAYRVPTDAPESDGTLAWDATTLVVAEVEGGGARGLGYTYADASAAAVVNGLLSEAVRGVDALDAPA
ASRAMRRALRNAGPAGVGGMALSAVDAALWDLHARLLGVPLLALLGRARPAVPAYGSGGFCSYAPERLREQLGGWAAAGF
PAVKMKVGRDPRADRERVRAAREAIGAAGLFVDANGAYGRKQALALAAAFADEGVTWLEEPVPSDDLAGLRLVRERAPAG
MDVAAGEYGHDPAYFRRMLEAGAVDVLQADATRCGGVTGFLAAAAVAAAFGVPLSSHCAPSLHATLMCAVERAAHAEWFH
DHERIEALLLEGAPRPSGGALAPDLSRPGLGLELRARDAERWAVA
>Mature_364_residues
ARGEATVERVHAAAYRVPTDAPESDGTLAWDATTLVVAEVEGGGARGLGYTYADASAAAVVNGLLSEAVRGVDALDAPAA
SRAMRRALRNAGPAGVGGMALSAVDAALWDLHARLLGVPLLALLGRARPAVPAYGSGGFCSYAPERLREQLGGWAAAGFP
AVKMKVGRDPRADRERVRAAREAIGAAGLFVDANGAYGRKQALALAAAFADEGVTWLEEPVPSDDLAGLRLVRERAPAGM
DVAAGEYGHDPAYFRRMLEAGAVDVLQADATRCGGVTGFLAAAAVAAAFGVPLSSHCAPSLHATLMCAVERAAHAEWFHD
HERIEALLLEGAPRPSGGALAPDLSRPGLGLELRARDAERWAVA

Specific function: Unknown

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=437, Percent_Identity=23.5697940503432, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI186972148, Length=258, Percent_Identity=27.1317829457364, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI226510960, Length=365, Percent_Identity=26.8493150684932, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI48994953, Length=276, Percent_Identity=26.0869565217391, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87081876, Length=248, Percent_Identity=28.2258064516129, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.1.2.2 [H]

Molecular weight: Translated: 37693; Mature: 37562

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGEATVERVHAAAYRVPTDAPESDGTLAWDATTLVVAEVEGGGARGLGYTYADASAAA
CCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCEEECCHHHHH
VVNGLLSEAVRGVDALDAPAASRAMRRALRNAGPAGVGGMALSAVDAALWDLHARLLGVP
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLALLGRARPAVPAYGSGGFCSYAPERLREQLGGWAAAGFPAVKMKVGRDPRADRERVRA
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCEECCCCCCEEEECCCCCCHHHHHHHH
AREAIGAAGLFVDANGAYGRKQALALAAAFADEGVTWLEEPVPSDDLAGLRLVRERAPAG
HHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHCCCC
MDVAAGEYGHDPAYFRRMLEAGAVDVLQADATRCGGVTGFLAAAAVAAAFGVPLSSHCAP
CEECCCCCCCCHHHHHHHHHHCCHHHHHHCHHHHCCHHHHHHHHHHHHHHCCCCHHCCCH
SLHATLMCAVERAAHAEWFHDHERIEALLLEGAPRPSGGALAPDLSRPGLGLELRARDAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
RWAVA
HHCCC
>Mature Secondary Structure 
ARGEATVERVHAAAYRVPTDAPESDGTLAWDATTLVVAEVEGGGARGLGYTYADASAAA
CCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCEEECCHHHHH
VVNGLLSEAVRGVDALDAPAASRAMRRALRNAGPAGVGGMALSAVDAALWDLHARLLGVP
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLALLGRARPAVPAYGSGGFCSYAPERLREQLGGWAAAGFPAVKMKVGRDPRADRERVRA
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCEECCCCCCEEEECCCCCCHHHHHHHH
AREAIGAAGLFVDANGAYGRKQALALAAAFADEGVTWLEEPVPSDDLAGLRLVRERAPAG
HHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHCCCC
MDVAAGEYGHDPAYFRRMLEAGAVDVLQADATRCGGVTGFLAAAAVAAAFGVPLSSHCAP
CEECCCCCCCCHHHHHHHHHHCCHHHHHHCHHHHCCHHHHHHHHHHHHHHCCCCHHCCCH
SLHATLMCAVERAAHAEWFHDHERIEALLLEGAPRPSGGALAPDLSRPGLGLELRARDAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
RWAVA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2831968; 2271624; 2215699; 1909893; 1892834; 7893689; 7893690 [H]