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The map label for this gene is ydaP [H]

Identifier: 220915974

GI number: 220915974

Start: 964048

End: 965829

Strand: Direct

Name: ydaP [H]

Synonym: A2cp1_0860

Alternate gene names: 220915974

Gene position: 964048-965829 (Clockwise)

Preceding gene: 220915973

Following gene: 220915975

Centisome position: 19.17

GC content: 73.63

Gene sequence:

>1782_bases
ATGAACGTCGGCGAGTTCCTGCTGCGCCGGCTGGTGGAGTGGGGCGTCCGCCGGGTGTTCGGCTATCCGGGCGACGGGAT
CAACGGGATCCTGGGCGCGTTCCGGACCGTGGACGCGCTGCGCTTCACCCAGGTCCGCCACGAGGAGATGGCGGCGTTCA
TGGCCTGCGCGCACGCGAAGTTCACCGGCGAGGTGGGCGTGTGCCTGGCCACCTCGGGCCCCGGCGCCATCCACCTCCTG
AACGGCCTGTACGACGCGCGCATGGACCACCAGCCGGTGGTCGCGATCGTGGGCCAGACCGCGCGCGCCGCCATGGGCGG
CTCCTACCAGCAGGAGGTGGACCTGCCCTCGCTGTTCAAGGACGTGGCGCACGAGTACGTGCAGACGCTGATGGTGCCGT
CGCAGGCCCGTCACCTGGTCGACCGGGCCGTGCGCATCGCGCGGGCGGAGCGGACCGTGACCGCGATCGTGGTGCCGAAC
GACGTGCAGGGCCTCGAGTACACGTCGCCCCCGCACGCCCACGCCACCGTCCACAGCGGGGTGGGCTGGGAGGCGCCGCG
GGTGGTCCCGGCCGCCCGCGACCTGCGCCGCGCGGCGGAGGTGCTGAACGCGGGGCGGCGCGTGGCGATGCTGGTGGGCG
CCGGCGCGCTCGGCGCGGCGCGCGAGGTCGCCGAGGTGGCCGACGTGCTCGGCGCCGGCGTCGCGAAGGCGCTGCTCGGC
AAGGCGGTGCTGCCCGACGACCTGCCCTGGGTGACCGGCGCCATCGGCCTGCTCGGCACCAAGCCCTCCTGGAACCTGAT
GAGCGGCTGCGACACGCTGCTGATGGTGGGCTCCTCGTTCCCGTACCCCGAGTTCCTGCCGAAGGAGGGGCAGGCGCGCG
GCGTCCAGGTCGACCTCGACGGCCGGATGCTCTCCATCCGCTATCCCATGGAGGTGAACCTGGTGGGCGACGCCGCCGAG
ACGCTCCAGGCGCTGCGGCCGCTGCTGCGGCGCAAGGAGCACGGGTCCTGGCGCGAGAAGATCGAGTCCGACGTGCGCCG
CTGGTGGAAGGTGCTGGAGGCGCGCGCCATGAACCCGGCCACGCCGGTGAACCCGCAGCGCGTCTTCTGGGAGCTGTCCC
GGCGCCTGCCCGATCGCGCCATCCTCTCGGCCGACTCCGGCTCGTCCGCGAACTGGTACGCGCGCGACGTGCGCGTGCGC
GAGGGGATGCTGGCGTCGCTCTCCGGCAACCTCGCGACCATGGGACCGGCCGTCCCGTACGCGATCGCCGCCAAGTTCGC
CTACCCGGACCGCGTGGCGGTGGCGCTGGCGGGCGACGGCGCCATGCAGATGAACGGGCTCGCCGAGCTGGCCACCATCG
CCCGCTACTGGCGCGAGTGGTCCGACCCGCGCCTGGTGGTCTTGGTGCTCGACAACGGCGACCTCAACCAGGTGACCTGG
GAGCAGCGGGTGATGGAGGGCGACCCGAAGCTCGAGGCGTCGCAGACGCTGCCGCCGGTGGACTACGCGGGCTTCGCTGC
CTCGCTCGGCCTCGCGGCGGTGAAGGTCGAGCGGCCGGAGGAGATCGGGCCGGCCTGGGACCGGGCGTTCGCCGCCGACC
GCCCGTGCCTGGTGCAGGCGCGGACGGATCCCAACGTGCCGCCGCTGCCGCCGCACATCACGCTGGAGCAGGCGAAGGGC
TACCTCGAGGCGATCCTGAAGGGCGACCCGGAGCGCGGCGCCATGGTCGTGCGCTCGTGGCGCGACGCGCTGGAGGGCGT
GCTGCCCCACCGGAAGGGCTGA

Upstream 100 bases:

>100_bases
CCGACGTGCCGGTGGTGGTGCAGCACACCCGGCTCGACGCCCGCCGCGCGGAGAGCGCGCTCATGACGACGCTCGCGTGG
GCGGCGGCGGAGGAGGGGGC

Downstream 100 bases:

>100_bases
CGTGGCGCGCGGCGAGGCCACCGTGGAGCGGGTGCACGCGGCCGCGTACCGCGTGCCCACCGACGCGCCCGAGTCGGACG
GCACGCTGGCCTGGGACGCG

Product: thiamine pyrophosphate protein

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MNVGEFLLRRLVEWGVRRVFGYPGDGINGILGAFRTVDALRFTQVRHEEMAAFMACAHAKFTGEVGVCLATSGPGAIHLL
NGLYDARMDHQPVVAIVGQTARAAMGGSYQQEVDLPSLFKDVAHEYVQTLMVPSQARHLVDRAVRIARAERTVTAIVVPN
DVQGLEYTSPPHAHATVHSGVGWEAPRVVPAARDLRRAAEVLNAGRRVAMLVGAGALGAAREVAEVADVLGAGVAKALLG
KAVLPDDLPWVTGAIGLLGTKPSWNLMSGCDTLLMVGSSFPYPEFLPKEGQARGVQVDLDGRMLSIRYPMEVNLVGDAAE
TLQALRPLLRRKEHGSWREKIESDVRRWWKVLEARAMNPATPVNPQRVFWELSRRLPDRAILSADSGSSANWYARDVRVR
EGMLASLSGNLATMGPAVPYAIAAKFAYPDRVAVALAGDGAMQMNGLAELATIARYWREWSDPRLVVLVLDNGDLNQVTW
EQRVMEGDPKLEASQTLPPVDYAGFAASLGLAAVKVERPEEIGPAWDRAFAADRPCLVQARTDPNVPPLPPHITLEQAKG
YLEAILKGDPERGAMVVRSWRDALEGVLPHRKG

Sequences:

>Translated_593_residues
MNVGEFLLRRLVEWGVRRVFGYPGDGINGILGAFRTVDALRFTQVRHEEMAAFMACAHAKFTGEVGVCLATSGPGAIHLL
NGLYDARMDHQPVVAIVGQTARAAMGGSYQQEVDLPSLFKDVAHEYVQTLMVPSQARHLVDRAVRIARAERTVTAIVVPN
DVQGLEYTSPPHAHATVHSGVGWEAPRVVPAARDLRRAAEVLNAGRRVAMLVGAGALGAAREVAEVADVLGAGVAKALLG
KAVLPDDLPWVTGAIGLLGTKPSWNLMSGCDTLLMVGSSFPYPEFLPKEGQARGVQVDLDGRMLSIRYPMEVNLVGDAAE
TLQALRPLLRRKEHGSWREKIESDVRRWWKVLEARAMNPATPVNPQRVFWELSRRLPDRAILSADSGSSANWYARDVRVR
EGMLASLSGNLATMGPAVPYAIAAKFAYPDRVAVALAGDGAMQMNGLAELATIARYWREWSDPRLVVLVLDNGDLNQVTW
EQRVMEGDPKLEASQTLPPVDYAGFAASLGLAAVKVERPEEIGPAWDRAFAADRPCLVQARTDPNVPPLPPHITLEQAKG
YLEAILKGDPERGAMVVRSWRDALEGVLPHRKG
>Mature_593_residues
MNVGEFLLRRLVEWGVRRVFGYPGDGINGILGAFRTVDALRFTQVRHEEMAAFMACAHAKFTGEVGVCLATSGPGAIHLL
NGLYDARMDHQPVVAIVGQTARAAMGGSYQQEVDLPSLFKDVAHEYVQTLMVPSQARHLVDRAVRIARAERTVTAIVVPN
DVQGLEYTSPPHAHATVHSGVGWEAPRVVPAARDLRRAAEVLNAGRRVAMLVGAGALGAAREVAEVADVLGAGVAKALLG
KAVLPDDLPWVTGAIGLLGTKPSWNLMSGCDTLLMVGSSFPYPEFLPKEGQARGVQVDLDGRMLSIRYPMEVNLVGDAAE
TLQALRPLLRRKEHGSWREKIESDVRRWWKVLEARAMNPATPVNPQRVFWELSRRLPDRAILSADSGSSANWYARDVRVR
EGMLASLSGNLATMGPAVPYAIAAKFAYPDRVAVALAGDGAMQMNGLAELATIARYWREWSDPRLVVLVLDNGDLNQVTW
EQRVMEGDPKLEASQTLPPVDYAGFAASLGLAAVKVERPEEIGPAWDRAFAADRPCLVQARTDPNVPPLPPHITLEQAKG
YLEAILKGDPERGAMVVRSWRDALEGVLPHRKG

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=574, Percent_Identity=23.5191637630662, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1787096, Length=583, Percent_Identity=31.2178387650086, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI87081685, Length=554, Percent_Identity=27.6173285198556, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1790104, Length=561, Percent_Identity=25.668449197861, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1786717, Length=545, Percent_Identity=26.4220183486239, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788716, Length=542, Percent_Identity=22.6937269372694, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17531299, Length=577, Percent_Identity=24.4367417677643, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17531301, Length=577, Percent_Identity=24.4367417677643, Blast_Score=92, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=597, Percent_Identity=24.7906197654941, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6320816, Length=512, Percent_Identity=21.6796875, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922626, Length=583, Percent_Identity=25.0428816466552, Blast_Score=112, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 64489; Mature: 64489

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVGEFLLRRLVEWGVRRVFGYPGDGINGILGAFRTVDALRFTQVRHEEMAAFMACAHAK
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FTGEVGVCLATSGPGAIHLLNGLYDARMDHQPVVAIVGQTARAAMGGSYQQEVDLPSLFK
CCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCHHCCCHHHHHH
DVAHEYVQTLMVPSQARHLVDRAVRIARAERTVTAIVVPNDVQGLEYTSPPHAHATVHSG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEEECC
VGWEAPRVVPAARDLRRAAEVLNAGRRVAMLVGAGALGAAREVAEVADVLGAGVAKALLG
CCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
KAVLPDDLPWVTGAIGLLGTKPSWNLMSGCDTLLMVGSSFPYPEFLPKEGQARGVQVDLD
CCCCCCCCCHHHHHHHHCCCCCCCCHHHCCHHHHEECCCCCCCCCCCCCCCCCCEEEEEC
GRMLSIRYPMEVNLVGDAAETLQALRPLLRRKEHGSWREKIESDVRRWWKVLEARAMNPA
CEEEEEECCEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
TPVNPQRVFWELSRRLPDRAILSADSGSSANWYARDVRVREGMLASLSGNLATMGPAVPY
CCCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEHHHHHHHCCHHHHHCCCCCCCCCCCCH
AIAAKFAYPDRVAVALAGDGAMQMNGLAELATIARYWREWSDPRLVVLVLDNGDLNQVTW
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHH
EQRVMEGDPKLEASQTLPPVDYAGFAASLGLAAVKVERPEEIGPAWDRAFAADRPCLVQA
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHCHHEEEECCCHHCCCHHHHHHCCCCCEEEEE
RTDPNVPPLPPHITLEQAKGYLEAILKGDPERGAMVVRSWRDALEGVLPHRKG
CCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNVGEFLLRRLVEWGVRRVFGYPGDGINGILGAFRTVDALRFTQVRHEEMAAFMACAHAK
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FTGEVGVCLATSGPGAIHLLNGLYDARMDHQPVVAIVGQTARAAMGGSYQQEVDLPSLFK
CCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCHHCCCHHHHHH
DVAHEYVQTLMVPSQARHLVDRAVRIARAERTVTAIVVPNDVQGLEYTSPPHAHATVHSG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEEECC
VGWEAPRVVPAARDLRRAAEVLNAGRRVAMLVGAGALGAAREVAEVADVLGAGVAKALLG
CCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
KAVLPDDLPWVTGAIGLLGTKPSWNLMSGCDTLLMVGSSFPYPEFLPKEGQARGVQVDLD
CCCCCCCCCHHHHHHHHCCCCCCCCHHHCCHHHHEECCCCCCCCCCCCCCCCCCEEEEEC
GRMLSIRYPMEVNLVGDAAETLQALRPLLRRKEHGSWREKIESDVRRWWKVLEARAMNPA
CEEEEEECCEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
TPVNPQRVFWELSRRLPDRAILSADSGSSANWYARDVRVREGMLASLSGNLATMGPAVPY
CCCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEHHHHHHHCCHHHHHCCCCCCCCCCCCH
AIAAKFAYPDRVAVALAGDGAMQMNGLAELATIARYWREWSDPRLVVLVLDNGDLNQVTW
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHH
EQRVMEGDPKLEASQTLPPVDYAGFAASLGLAAVKVERPEEIGPAWDRAFAADRPCLVQA
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHCHHEEEECCCHHCCCHHHHHHCCCCCEEEEE
RTDPNVPPLPPHITLEQAKGYLEAILKGDPERGAMVVRSWRDALEGVLPHRKG
CCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0017 {lecithin}} 0.03 {sodium} 11 {pyruvate}} 10 {pyruvate}} 0.0022 {lysolecithin}} 2 {ferricyanide}} 0.0012 {thiamine} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]