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The map label for this gene is dnaG [H]

Identifier: 220915873

GI number: 220915873

Start: 847171

End: 849024

Strand: Direct

Name: dnaG [H]

Synonym: A2cp1_0757

Alternate gene names: 220915873

Gene position: 847171-849024 (Clockwise)

Preceding gene: 220915872

Following gene: 220915874

Centisome position: 16.84

GC content: 75.51

Gene sequence:

>1854_bases
GTGGTGGCCGTGATCGGCCGCCACATGGAGCTGAAGCGATCGGGCAGGACGTGGAAGGGCAACTGCATCTTCCACGGGGA
GCGGACGCCGAGCTTTCACGTCTACCCCGAGGACAAGCACTTCAAGTGCTACGGCTGCGGGGCGTACGGCGACGTCTTCA
CGTTCCTGCAGCGGCTCGAGGGGAAGGAGTTTCCCGAGGTGGTCAGGGCGCTCGCGCAGGAGGTCGGCGTCGAGATCCCG
GAGGCGGCCGAGGAGGACTCGGCCGAGGCGAGGGCGAAGCGCAAGGAGCGCAACGAGGTCCTCGCCGCGAGCGACGCCGC
CGCGCGCTACTGGGCGGCGCGGCTCGCCAGCCGCTTCGGCGCGCCGGCGCGGGCGTACCTCGAGCGGCGCGGCGTCACCG
AGGAGTCGGTCCGGCGCTTCCGGCTGGGGGTGGCGGCCGACGCGTGGAACGACCTCGCGCCGCGGCTGAAGGAGAAGGGC
ATCGGGGTCGCGGCGCTGCAGCGCGCCGGGCTGGTCATCGAGAGGGAGAGGGACGGCGGCGGCACGTACGACCGCTTCCG
CAACCGCCTGATGTTTCCCATCGCCGGCATGGACGGGCAGGTCATCGGCTTCGGCGGGCGCGCGCTCGGCGAGGAGCAGG
GCGCGAAGTACATCAACACGCCCGAGACCCCGCTCTACAAGAAGTCGAAGGTGCTCTACGGCCTCGACCTGGCCCGCGAG
ACCATCCGCAAGACGCGCAGCGCGGTCCTGGTCGAGGGCTACTTCGACGTGATCGGGCTGCACCAGGTGGGCGTGACCGG
CGCGGTGGCGGTGTGCGGCACCGCCCTCACCCCCGAGCACGTGGAGCTGCTCTCCCGCTGCGACTGCCGCGAGGTCGCCG
TGCTGTTCGACGGCGACGTGGCCGGCCTGGCCGCGCCGGCCAAGGCCGCGCAGGCGCTGTTCCCGGCGGGCATGGCCGGC
AAGGTGGCGGTGCTCCCGTCCGCCGCGGGCAAGAGCGATCCCGACGAGTACGCCCGCGCGAACGGGCGCGCCGGGGTCGA
CGCGCTGCTGGCGGCCGCCGTCCCCCTCTCGGAGTTCCTGGTGGACCGGGCGGTCGAGCGGGCGTGCGGCGACCGGCCGC
GGGAGGCCGCGCTCGAGCGGAAGCTCGCCGCGGTTCGCGAGCTCGAGCCGTTCGTGCGGATGATGCCGGAGGGTCTCGCG
CGTTCCGTCTTCGAGGACATGATCGCGCGCCGGCTCGACCTCGACCCGGGTGCGCTCAGGACCGAGCTGTCCGCCGAGCG
GCGGCGGCCGGGCGTCCCGGCGCCCGCCCCGACGGGTCACCCGGAGCGGCCCTCGGGCGCCCCATCCCGCCCCGCCCCGT
CCGCGGGGCCGGCGCGCGGTGGGCCGTCCGCCCACGCCAGGGTGCGGGTGCTGCTGCCCGGCCCGGCCGCGGACGCGCTG
GCGCTGCTGGCCGCCTTCCCCGATCTGGGGCCGGTGGCGGAGGAGGAGCGCCTTCCGCAGCTCCTGCCGCCCGGGCCGCT
GGCGGACCTGGCCCGCGACCTGGTGCGCGGGCCGGTCGCGCTCGACGACGCGCTGGCGCGGCTGTCCTCCGGCGCCGACG
AGGCCACGGCCCGGCGGTTCCGCACGCTGGTGGGGCCCGGCCGGCCGAGGCCGGAGGAGGCGGAGCGCGAGCTCCGGAAG
GCGGCGGTCAAGGCGACGCTGGAGCGCCTCGACCAGGAGTACGGGGAGGCGCTGCGGCGGGTCACCAAGGCCGGCAGCGC
CGCGGCGGACGGGCTGGACGTGACCGCCCAGCGCCTCATCAACCAGCGCCGCGACCTGCAGAGACGCTTGCGTTCCCTGG
AACGTCCCGGATGA

Upstream 100 bases:

>100_bases
CCGCCCGGCGGTGGCCGGGCGCCGTGCGTGCCGCGAGCGGTAGCGGAGGCGCAACCTGATCCCCGACGCGGTCATCGACC
AGATCCGCGAGCGCGTCGAC

Downstream 100 bases:

>100_bases
AAGGCAAGCTCCGAAGCATTCTGGATACATTGGCCGGGCTCACCCGGCCGAAAAACGTTACGAAAGAGCACCACCGGGCC
GCCGAGCCGTCGAAGGAGCC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MVAVIGRHMELKRSGRTWKGNCIFHGERTPSFHVYPEDKHFKCYGCGAYGDVFTFLQRLEGKEFPEVVRALAQEVGVEIP
EAAEEDSAEARAKRKERNEVLAASDAAARYWAARLASRFGAPARAYLERRGVTEESVRRFRLGVAADAWNDLAPRLKEKG
IGVAALQRAGLVIERERDGGGTYDRFRNRLMFPIAGMDGQVIGFGGRALGEEQGAKYINTPETPLYKKSKVLYGLDLARE
TIRKTRSAVLVEGYFDVIGLHQVGVTGAVAVCGTALTPEHVELLSRCDCREVAVLFDGDVAGLAAPAKAAQALFPAGMAG
KVAVLPSAAGKSDPDEYARANGRAGVDALLAAAVPLSEFLVDRAVERACGDRPREAALERKLAAVRELEPFVRMMPEGLA
RSVFEDMIARRLDLDPGALRTELSAERRRPGVPAPAPTGHPERPSGAPSRPAPSAGPARGGPSAHARVRVLLPGPAADAL
ALLAAFPDLGPVAEEERLPQLLPPGPLADLARDLVRGPVALDDALARLSSGADEATARRFRTLVGPGRPRPEEAERELRK
AAVKATLERLDQEYGEALRRVTKAGSAAADGLDVTAQRLINQRRDLQRRLRSLERPG

Sequences:

>Translated_617_residues
MVAVIGRHMELKRSGRTWKGNCIFHGERTPSFHVYPEDKHFKCYGCGAYGDVFTFLQRLEGKEFPEVVRALAQEVGVEIP
EAAEEDSAEARAKRKERNEVLAASDAAARYWAARLASRFGAPARAYLERRGVTEESVRRFRLGVAADAWNDLAPRLKEKG
IGVAALQRAGLVIERERDGGGTYDRFRNRLMFPIAGMDGQVIGFGGRALGEEQGAKYINTPETPLYKKSKVLYGLDLARE
TIRKTRSAVLVEGYFDVIGLHQVGVTGAVAVCGTALTPEHVELLSRCDCREVAVLFDGDVAGLAAPAKAAQALFPAGMAG
KVAVLPSAAGKSDPDEYARANGRAGVDALLAAAVPLSEFLVDRAVERACGDRPREAALERKLAAVRELEPFVRMMPEGLA
RSVFEDMIARRLDLDPGALRTELSAERRRPGVPAPAPTGHPERPSGAPSRPAPSAGPARGGPSAHARVRVLLPGPAADAL
ALLAAFPDLGPVAEEERLPQLLPPGPLADLARDLVRGPVALDDALARLSSGADEATARRFRTLVGPGRPRPEEAERELRK
AAVKATLERLDQEYGEALRRVTKAGSAAADGLDVTAQRLINQRRDLQRRLRSLERPG
>Mature_617_residues
MVAVIGRHMELKRSGRTWKGNCIFHGERTPSFHVYPEDKHFKCYGCGAYGDVFTFLQRLEGKEFPEVVRALAQEVGVEIP
EAAEEDSAEARAKRKERNEVLAASDAAARYWAARLASRFGAPARAYLERRGVTEESVRRFRLGVAADAWNDLAPRLKEKG
IGVAALQRAGLVIERERDGGGTYDRFRNRLMFPIAGMDGQVIGFGGRALGEEQGAKYINTPETPLYKKSKVLYGLDLARE
TIRKTRSAVLVEGYFDVIGLHQVGVTGAVAVCGTALTPEHVELLSRCDCREVAVLFDGDVAGLAAPAKAAQALFPAGMAG
KVAVLPSAAGKSDPDEYARANGRAGVDALLAAAVPLSEFLVDRAVERACGDRPREAALERKLAAVRELEPFVRMMPEGLA
RSVFEDMIARRLDLDPGALRTELSAERRRPGVPAPAPTGHPERPSGAPSRPAPSAGPARGGPSAHARVRVLLPGPAADAL
ALLAAFPDLGPVAEEERLPQLLPPGPLADLARDLVRGPVALDDALARLSSGADEATARRFRTLVGPGRPRPEEAERELRK
AAVKATLERLDQEYGEALRRVTKAGSAAADGLDVTAQRLINQRRDLQRRLRSLERPG

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=362, Percent_Identity=35.6353591160221, Blast_Score=232, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 66590; Mature: 66590

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVIGRHMELKRSGRTWKGNCIFHGERTPSFHVYPEDKHFKCYGCGAYGDVFTFLQRLE
CEEEECCCHHHHHCCCCEECCEEEECCCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHC
GKEFPEVVRALAQEVGVEIPEAAEEDSAEARAKRKERNEVLAASDAAARYWAARLASRFG
CCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APARAYLERRGVTEESVRRFRLGVAADAWNDLAPRLKEKGIGVAALQRAGLVIERERDGG
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCC
GTYDRFRNRLMFPIAGMDGQVIGFGGRALGEEQGAKYINTPETPLYKKSKVLYGLDLARE
CCHHHHHCCEEEEEECCCCCEEECCCHHHCHHHCCCCCCCCCCCCHHCCHHHHHHHHHHH
TIRKTRSAVLVEGYFDVIGLHQVGVTGAVAVCGTALTPEHVELLSRCDCREVAVLFDGDV
HHHHHHHHEEEEHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHCCHHHEEEEECCCH
AGLAAPAKAAQALFPAGMAGKVAVLPSAAGKSDPDEYARANGRAGVDALLAAAVPLSEFL
HHHCCHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHCCHHHHH
VDRAVERACGDRPREAALERKLAAVRELEPFVRMMPEGLARSVFEDMIARRLDLDPGALR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
TELSAERRRPGVPAPAPTGHPERPSGAPSRPAPSAGPARGGPSAHARVRVLLPGPAADAL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHH
ALLAAFPDLGPVAEEERLPQLLPPGPLADLARDLVRGPVALDDALARLSSGADEATARRF
HHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
RTLVGPGRPRPEEAERELRKAAVKATLERLDQEYGEALRRVTKAGSAAADGLDVTAQRLI
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
NQRRDLQRRLRSLERPG
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVAVIGRHMELKRSGRTWKGNCIFHGERTPSFHVYPEDKHFKCYGCGAYGDVFTFLQRLE
CEEEECCCHHHHHCCCCEECCEEEECCCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHC
GKEFPEVVRALAQEVGVEIPEAAEEDSAEARAKRKERNEVLAASDAAARYWAARLASRFG
CCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APARAYLERRGVTEESVRRFRLGVAADAWNDLAPRLKEKGIGVAALQRAGLVIERERDGG
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCC
GTYDRFRNRLMFPIAGMDGQVIGFGGRALGEEQGAKYINTPETPLYKKSKVLYGLDLARE
CCHHHHHCCEEEEEECCCCCEEECCCHHHCHHHCCCCCCCCCCCCHHCCHHHHHHHHHHH
TIRKTRSAVLVEGYFDVIGLHQVGVTGAVAVCGTALTPEHVELLSRCDCREVAVLFDGDV
HHHHHHHHEEEEHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHCCHHHEEEEECCCH
AGLAAPAKAAQALFPAGMAGKVAVLPSAAGKSDPDEYARANGRAGVDALLAAAVPLSEFL
HHHCCHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHCCHHHHH
VDRAVERACGDRPREAALERKLAAVRELEPFVRMMPEGLARSVFEDMIARRLDLDPGALR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
TELSAERRRPGVPAPAPTGHPERPSGAPSRPAPSAGPARGGPSAHARVRVLLPGPAADAL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHH
ALLAAFPDLGPVAEEERLPQLLPPGPLADLARDLVRGPVALDDALARLSSGADEATARRF
HHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
RTLVGPGRPRPEEAERELRKAAVKATLERLDQEYGEALRRVTKAGSAAADGLDVTAQRLI
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
NQRRDLQRRLRSLERPG
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA