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The map label for this gene is rpoD [H]

Identifier: 220915874

GI number: 220915874

Start: 849021

End: 851429

Strand: Direct

Name: rpoD [H]

Synonym: A2cp1_0758

Alternate gene names: 220915874

Gene position: 849021-851429 (Clockwise)

Preceding gene: 220915873

Following gene: 220915875

Centisome position: 16.88

GC content: 68.49

Gene sequence:

>2409_bases
ATGAAAGGCAAGCTCCGAAGCATTCTGGATACATTGGCCGGGCTCACCCGGCCGAAAAACGTTACGAAAGAGCACCACCG
GGCCGCCGAGCCGTCGAAGGAGCCGAAGCCGATGACCACGAAGGGCCACGCCGCGAAGAAGCACGTGAAGAAGGCAGCGC
CGAAGCAGAAGGCGAAGGCCGGCGGCCGCAAGGAAGAGCGCCAGGCCAAGCCCGCGCCCAGGGCGCCTGCGCGCGCGGTG
GCGAAGCCGCCGGTCAAGCGCGCCAAGGCCGAGCGCGAGAAGCCGGAGAAGGTCGAGAAGCCCGAGAAGGCCGAGCGGCA
CGAGAAGGCCGGCGAGAAGCCCGAGAAGGCCCGCAAGAAGGCGCCCGAGGCTGCGGCCGCCGAGCCCGCCCGCCCGGCGG
GTCGCCCCGGCAAGCACCGTCGCCCCACCGACGAGGCCGAGCCCGAGACCGAGCGCGCCGAGGCGGACGCCGACGACGAC
GACGACGACGAGCTCGACGAGCTCCCGCAGGACGACATGAGCGACATCACCGAGCGGTCCGAGGTGAAGGAGCTCATGGC
GCGCGGGCGCGAGAAGGGCTTCCTCACCTACGACGAGGTGAACGACGCGCTGCCCTCGGACATCGGCTCGGACCAGATCG
ACGACGTCATGTCGATGTTCGGCGAGCACGACATCGAGGTCGTGGACGGCGCCGAGAAGAAGATGAAGCTCCCGGACAAG
CCGCCCGAGCCCGAGGCCAAGGCGGACGCCGAGGAGGAGAAGGAGGAGGAGGACGACGCCGGCTACGGCAAGTCCAACGA
CCCGGTGCGCATGTACCTGCGCAAGATGGGGTCCGTCTCCCTCCTCACGCGCGAGGGCGAGGTGGAGATCGCGAAGCGCA
TCGAGGAGGGCGAGAAGGAGATCCTCGGCGCGGTGCTCTCGTCGTCGATCGCCATCCGCGAGATCCTCGAGCTGGGCGAG
CGGCTCCGCAAGGGCAAGATCCGCGTCCGCGAGATCATCAAGGACGCCGGCGACGAGCAGCAGGCCGAGGCGGAGGAGCC
CGAGGAGGAGCTGGACGAGGCCGCCGAGGCGCCGGAGGGTCAGGGCGACGCCGAGGCCGAGGCCGGCGACGGCGCCGCGC
CGGCCGAGGGCGCCGAGCCGGAGAAGCCCCGCATCCCGAAGGAGGAGGAGCGCAAGGTCGAGCACGTGCTGAAGCACATC
GACCGGATCCGCAAGCTGGAGCGCGACGTCGCGAAGGTCCGCGAGACGCTCCAGGGCGGCAAGAAGCTCTCCGAGGTGAA
GCGCAAAGAGCTGAAGGGCGAGATCCGCGCCATCGAGGCGGAGATGATGGAGAACCTCGAGGCGATCCAGCTCAACAAGA
AGCAGATCGACCGGATCGTCCTGCGCCTGAAGAGCTTCATCAAGCGCGTGGAGGAGGCGGAGCGCGAGTTCTTCGACTGC
GAGCGCCGCACCGGCGTGCCGGTGAAGGACCTGCGGCGCCTGCTCCGCGAGACGCGCGAGGACGAGGCCGAGCGGAAGAA
GCTCGCGAAGAAGCTCCGCTGCGCGCTCGACGAGGTGGAGGAGCTGGACCGCACGCTGCGCTCGGCCGGCCGCAAGATCC
TGCGCGTGCAGGAGGAGGCGGAGGTGCCGGTGGAGGAGCTGCGCCGGACCTACCGCATGATCGGCGACGGCGAGCGGAAG
GCGGAGCGCGCCAAGACCGAGCTGGTCGAGGCGAACCTGCGCCTGGTGGTCTCGATCGCGAAGAAGTACACGAACCGCGG
CCTGCAGTTCCTCGACCTGATCCAGGAGGGGAACATCGGCCTGATGAAGGCGGTCGACAAGTTCGAGTACAAGCGCGGCT
ACAAGTTCTCGACCTACGCCACCTGGTGGATCCGCCAGGCGATCACGCGCGCCATCGCGGACCAGGCGCGGACCATCCGC
ATCCCGGTCCACATGATCGAGACCATCAACAAGCTCATCCGCACCAGCCGCTACCTGGTGCAGGAGCTGGGGCGCGAGCC
GACGCCGGAGGAGATCGCCGAGAAGATGGAGCTCCCGCTCGACAAGGTCCGCAAGGTCCTGAAGATCGCGAAGGAGCCCA
TCTCGCTCGAGACGCCCATCGGCGAGGAGGAGGACAGCCACCTCGGGGACTTCATCGAGGACAAGTCGCTGGTCTCCCCG
TCGGACGCGGTCATCAACATGAACCTGGCCGAGCAGACCAGGAAGGTGCTCGCCACGCTGACCCCGCGCGAGGAGAAGGT
GCTCCGCATGCGGTTCGGCATCGGCGAGAAGAGCGATCACACGCTCGAGGAGGTCGGCCAGGACTTCGAGGTCACACGCG
AGCGCATCCGCCAGATCGAGGCGAAGGCGCTGCGGAAGCTGCGCCACCCGTCGCGGTCGAAGCGGCTCAAGAGCTTCGTG
GAGAGCTGA

Upstream 100 bases:

>100_bases
CCGGCAGCGCCGCGGCGGACGGGCTGGACGTGACCGCCCAGCGCCTCATCAACCAGCGCCGCGACCTGCAGAGACGCTTG
CGTTCCCTGGAACGTCCCGG

Downstream 100 bases:

>100_bases
TCGACCCCGTCTCGGGCGACGCGAATATGATAGAGGGTGCCTGCCGGGGCCTCGGGCCCATAGCTCAACGGTCAGAGCGC
TCGGCTCATAACCGATGCGA

Product: RpoD subfamily RNA polymerase sigma-70 subunit

Products: NA

Alternate protein names: Sigma-80 [H]

Number of amino acids: Translated: 802; Mature: 802

Protein sequence:

>802_residues
MKGKLRSILDTLAGLTRPKNVTKEHHRAAEPSKEPKPMTTKGHAAKKHVKKAAPKQKAKAGGRKEERQAKPAPRAPARAV
AKPPVKRAKAEREKPEKVEKPEKAERHEKAGEKPEKARKKAPEAAAAEPARPAGRPGKHRRPTDEAEPETERAEADADDD
DDDELDELPQDDMSDITERSEVKELMARGREKGFLTYDEVNDALPSDIGSDQIDDVMSMFGEHDIEVVDGAEKKMKLPDK
PPEPEAKADAEEEKEEEDDAGYGKSNDPVRMYLRKMGSVSLLTREGEVEIAKRIEEGEKEILGAVLSSSIAIREILELGE
RLRKGKIRVREIIKDAGDEQQAEAEEPEEELDEAAEAPEGQGDAEAEAGDGAAPAEGAEPEKPRIPKEEERKVEHVLKHI
DRIRKLERDVAKVRETLQGGKKLSEVKRKELKGEIRAIEAEMMENLEAIQLNKKQIDRIVLRLKSFIKRVEEAEREFFDC
ERRTGVPVKDLRRLLRETREDEAERKKLAKKLRCALDEVEELDRTLRSAGRKILRVQEEAEVPVEELRRTYRMIGDGERK
AERAKTELVEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIR
IPVHMIETINKLIRTSRYLVQELGREPTPEEIAEKMELPLDKVRKVLKIAKEPISLETPIGEEEDSHLGDFIEDKSLVSP
SDAVINMNLAEQTRKVLATLTPREEKVLRMRFGIGEKSDHTLEEVGQDFEVTRERIRQIEAKALRKLRHPSRSKRLKSFV
ES

Sequences:

>Translated_802_residues
MKGKLRSILDTLAGLTRPKNVTKEHHRAAEPSKEPKPMTTKGHAAKKHVKKAAPKQKAKAGGRKEERQAKPAPRAPARAV
AKPPVKRAKAEREKPEKVEKPEKAERHEKAGEKPEKARKKAPEAAAAEPARPAGRPGKHRRPTDEAEPETERAEADADDD
DDDELDELPQDDMSDITERSEVKELMARGREKGFLTYDEVNDALPSDIGSDQIDDVMSMFGEHDIEVVDGAEKKMKLPDK
PPEPEAKADAEEEKEEEDDAGYGKSNDPVRMYLRKMGSVSLLTREGEVEIAKRIEEGEKEILGAVLSSSIAIREILELGE
RLRKGKIRVREIIKDAGDEQQAEAEEPEEELDEAAEAPEGQGDAEAEAGDGAAPAEGAEPEKPRIPKEEERKVEHVLKHI
DRIRKLERDVAKVRETLQGGKKLSEVKRKELKGEIRAIEAEMMENLEAIQLNKKQIDRIVLRLKSFIKRVEEAEREFFDC
ERRTGVPVKDLRRLLRETREDEAERKKLAKKLRCALDEVEELDRTLRSAGRKILRVQEEAEVPVEELRRTYRMIGDGERK
AERAKTELVEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIR
IPVHMIETINKLIRTSRYLVQELGREPTPEEIAEKMELPLDKVRKVLKIAKEPISLETPIGEEEDSHLGDFIEDKSLVSP
SDAVINMNLAEQTRKVLATLTPREEKVLRMRFGIGEKSDHTLEEVGQDFEVTRERIRQIEAKALRKLRHPSRSKRLKSFV
ES
>Mature_802_residues
MKGKLRSILDTLAGLTRPKNVTKEHHRAAEPSKEPKPMTTKGHAAKKHVKKAAPKQKAKAGGRKEERQAKPAPRAPARAV
AKPPVKRAKAEREKPEKVEKPEKAERHEKAGEKPEKARKKAPEAAAAEPARPAGRPGKHRRPTDEAEPETERAEADADDD
DDDELDELPQDDMSDITERSEVKELMARGREKGFLTYDEVNDALPSDIGSDQIDDVMSMFGEHDIEVVDGAEKKMKLPDK
PPEPEAKADAEEEKEEEDDAGYGKSNDPVRMYLRKMGSVSLLTREGEVEIAKRIEEGEKEILGAVLSSSIAIREILELGE
RLRKGKIRVREIIKDAGDEQQAEAEEPEEELDEAAEAPEGQGDAEAEAGDGAAPAEGAEPEKPRIPKEEERKVEHVLKHI
DRIRKLERDVAKVRETLQGGKKLSEVKRKELKGEIRAIEAEMMENLEAIQLNKKQIDRIVLRLKSFIKRVEEAEREFFDC
ERRTGVPVKDLRRLLRETREDEAERKKLAKKLRCALDEVEELDRTLRSAGRKILRVQEEAEVPVEELRRTYRMIGDGERK
AERAKTELVEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIR
IPVHMIETINKLIRTSRYLVQELGREPTPEEIAEKMELPLDKVRKVLKIAKEPISLETPIGEEEDSHLGDFIEDKSLVSP
SDAVINMNLAEQTRKVLATLTPREEKVLRMRFGIGEKSDHTLEEVGQDFEVTRERIRQIEAKALRKLRHPSRSKRLKSFV
ES

Specific function: Also required for extracellular A-signal production. The A-signal is the first in a series of extracellular signals that are required for myxspore differentiation in M.xanthus. It is thought to act as a cell-density signal during early development to ensu

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=646, Percent_Identity=47.6780185758514, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1789098, Length=230, Percent_Identity=44.3478260869565, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1789871, Length=292, Percent_Identity=28.7671232876712, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.7128712871287, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 91163; Mature: 91163

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGKLRSILDTLAGLTRPKNVTKEHHRAAEPSKEPKPMTTKGHAAKKHVKKAAPKQKAKA
CCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHC
GGRKEERQAKPAPRAPARAVAKPPVKRAKAEREKPEKVEKPEKAERHEKAGEKPEKARKK
CCCCHHHHCCCCCCCCHHHHCCCCHHHHHHHHCCCHHCCCCHHHHHHHHCCCCHHHHHHC
APEAAAAEPARPAGRPGKHRRPTDEAEPETERAEADADDDDDDELDELPQDDMSDITERS
CCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHH
EVKELMARGREKGFLTYDEVNDALPSDIGSDQIDDVMSMFGEHDIEVVDGAEKKMKLPDK
HHHHHHHCCCCCCCEEHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEECCCHHHCCCCCC
PPEPEAKADAEEEKEEEDDAGYGKSNDPVRMYLRKMGSVSLLTREGEVEIAKRIEEGEKE
CCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHH
ILGAVLSSSIAIREILELGERLRKGKIRVREIIKDAGDEQQAEAEEPEEELDEAAEAPEG
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHCCCCC
QGDAEAEAGDGAAPAEGAEPEKPRIPKEEERKVEHVLKHIDRIRKLERDVAKVRETLQGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKLSEVKRKELKGEIRAIEAEMMENLEAIQLNKKQIDRIVLRLKSFIKRVEEAEREFFDC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERRTGVPVKDLRRLLRETREDEAERKKLAKKLRCALDEVEELDRTLRSAGRKILRVQEEA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVPVEELRRTYRMIGDGERKAERAKTELVEANLRLVVSIAKKYTNRGLQFLDLIQEGNIG
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCH
LMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRYLV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHHHH
QELGREPTPEEIAEKMELPLDKVRKVLKIAKEPISLETPIGEEEDSHLGDFIEDKSLVSP
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
SDAVINMNLAEQTRKVLATLTPREEKVLRMRFGIGEKSDHTLEEVGQDFEVTRERIRQIE
CCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHH
AKALRKLRHPSRSKRLKSFVES
HHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MKGKLRSILDTLAGLTRPKNVTKEHHRAAEPSKEPKPMTTKGHAAKKHVKKAAPKQKAKA
CCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHC
GGRKEERQAKPAPRAPARAVAKPPVKRAKAEREKPEKVEKPEKAERHEKAGEKPEKARKK
CCCCHHHHCCCCCCCCHHHHCCCCHHHHHHHHCCCHHCCCCHHHHHHHHCCCCHHHHHHC
APEAAAAEPARPAGRPGKHRRPTDEAEPETERAEADADDDDDDELDELPQDDMSDITERS
CCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHH
EVKELMARGREKGFLTYDEVNDALPSDIGSDQIDDVMSMFGEHDIEVVDGAEKKMKLPDK
HHHHHHHCCCCCCCEEHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEECCCHHHCCCCCC
PPEPEAKADAEEEKEEEDDAGYGKSNDPVRMYLRKMGSVSLLTREGEVEIAKRIEEGEKE
CCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHH
ILGAVLSSSIAIREILELGERLRKGKIRVREIIKDAGDEQQAEAEEPEEELDEAAEAPEG
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHCCCCC
QGDAEAEAGDGAAPAEGAEPEKPRIPKEEERKVEHVLKHIDRIRKLERDVAKVRETLQGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKLSEVKRKELKGEIRAIEAEMMENLEAIQLNKKQIDRIVLRLKSFIKRVEEAEREFFDC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERRTGVPVKDLRRLLRETREDEAERKKLAKKLRCALDEVEELDRTLRSAGRKILRVQEEA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVPVEELRRTYRMIGDGERKAERAKTELVEANLRLVVSIAKKYTNRGLQFLDLIQEGNIG
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCH
LMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRYLV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHHHH
QELGREPTPEEIAEKMELPLDKVRKVLKIAKEPISLETPIGEEEDSHLGDFIEDKSLVSP
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
SDAVINMNLAEQTRKVLATLTPREEKVLRMRFGIGEKSDHTLEEVGQDFEVTRERIRQIE
CCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHH
AKALRKLRHPSRSKRLKSFVES
HHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2104614 [H]