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The map label for this gene is tapB [H]

Identifier: 220915775

GI number: 220915775

Start: 729837

End: 731543

Strand: Direct

Name: tapB [H]

Synonym: A2cp1_0656

Alternate gene names: 220915775

Gene position: 729837-731543 (Clockwise)

Preceding gene: 220915774

Following gene: 220915776

Centisome position: 14.51

GC content: 66.32

Gene sequence:

>1707_bases
ATGTCCGGACGGCTCGGCGAACTGCTGGTGCGGGAGAACCTCATCTCCCTGCAGCAGCTGCAGAAGGCCCAGGACGAGCA
GCGCAAGACCGGAGGGCGCATCGGCTCGCTGCTGGTGAAGCAGGGCGCCATCGCCGAGAGCGACCTCACCGGCTTCCTCT
CGAAGCAGTACGGCGTCCCGGCGATCTCGCTGAAGGACTTCGACATCGACGACGAGGTGCTGAAGCTCGTCCCGAAGGCG
GCCGCGGAGAAGCATCAGGTCATCCCGGTGAACCGCGCCGGCTCCTCGCTCATCGTGGCGATGAGCGACCCGTCCAACAT
CTTCGCCATCGACGACGTCAAGTTCCTCACCGGCTACAACGTCGAGGTGGTGGTCGCCTCCGAGCAGGCGATCCGCGAGG
CGATCGAGAAGTACTACGCGGAGAAGGGGCCCGACCTCGACGAGGTGATGCAGGGCTTCGACGACTCGGAGGTCGCGATC
GTCGAGGCCGACGGCGACGACATGAACGTCGTCGACCTCGAGAAGAGCGCGGAGGAGGCGCCGGTCGTCAAGCTGGTGAA
CCTCATCCTGCTCGACGCCATCAAGAAGGGGGCCTCGGACATCCACGTCGAGCCCTACGAGAAGGACTTCCGCGTCCGCT
TCCGCATCGACGGCGTGCTGTACGAGGTGATGAAGCCGCCCATGAAGCTGCGGAACGCCATCATCTCGCGCCTCAAGATC
ATGTCGGAGCTCGACATCTCCGAGCGCCGGCTGCCGCAGGATGGGCGCATCAAGCTGAAGCTGGGCAAGGGCAAGGAGAT
GGACTTCCGCGTCTCCATCTGCCCGACGCTGTTCGGCGAGAAGGTGGTGATGCGCCTCCTCGACAAGTCGAACCTGCAGC
TCGACATGACGAAGCTCGGGTTCGAGGAGCAGCAGCTCAAGGACTTCATGGAGGCGATCGACCGGCCCTACGGCATGGTC
CTCGTCACCGGCCCGACCGGCTCCGGCAAAACCACCACGCTCTACTCGGCGCTCTCCAAGCTGAACGAGGTCGCCTGGAA
CATCTCCACCGCCGAGGACCCGGTGGAGTTCAACTTCTTCGGCATCAACCAGGTCCAGATGCACGAGGACATCGGCCTCA
ACTTCGCGGCCGCGCTGCGCAGCTTCCTGCGGCAGGACCCCGACATCATCATGGTCGGCGAGATCCGCGACTTCGAGACC
GCCGAGATCGGCGTGAAGGCCGCGCTCACCGGGCACCTCGTGCTCTCGACGCTGCACACCAACGACGCGCCCGGCACCGT
CTCCCGCCTCCTCAACATGGGCATCGAGCCGTTCCTGGTGACCGCGTCCCTGAACGCGATCGTGGCGCAGCGGCTCTGCC
GCCGGCTCTGCCCCGAGTGCCGCAAGGCGGTGGCGCCGGACGAGCAGGCGCTGCTCGACGCCGGGTTCCCCGCCGAGGAG
ATCGGCACGTTCCAGGTGTACGAGCCGGGCGGCTGCAAGGCCTGCAACGACCGCGGCTACAAGGGGCGCGTGGCGGTGTA
CGAGGTGATGCCGCTCTGGGACGGGCTGAAGGAGCTCGTCATCCAGGGCTGCTCCGCCGCCGAGCTGAAGCAGGAGGCGA
TCCGCCTCGGCTTCCGCACGCTGCGCATGAGCGCGCTCAACAAGGTGAAGACCGGCATGACCAGCCTCGCCGAGGCGGTC
GGCAACACCGCGCCGGACAAGTTCTAG

Upstream 100 bases:

>100_bases
GCCCGTTCCTTGACCCTCCCAGGAACCGCCTGTTAAGGTCGCTGGGCTCCCCGCGCGGATCCCGCCGCGCTCCGGCGAAG
GCAACGAGGGCTAAGACGAC

Downstream 100 bases:

>100_bases
GAGACGGCCAACCTTGGCGAACCTGCACCAGCTCCTGAAGGCGATGGTCGAGAAGGGGGCCTCCGACCTCCACGTCACCA
CCGGCTCCCCGCCCCAGCTC

Product: type IV-A pilus assembly ATPase PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MSGRLGELLVRENLISLQQLQKAQDEQRKTGGRIGSLLVKQGAIAESDLTGFLSKQYGVPAISLKDFDIDDEVLKLVPKA
AAEKHQVIPVNRAGSSLIVAMSDPSNIFAIDDVKFLTGYNVEVVVASEQAIREAIEKYYAEKGPDLDEVMQGFDDSEVAI
VEADGDDMNVVDLEKSAEEAPVVKLVNLILLDAIKKGASDIHVEPYEKDFRVRFRIDGVLYEVMKPPMKLRNAIISRLKI
MSELDISERRLPQDGRIKLKLGKGKEMDFRVSICPTLFGEKVVMRLLDKSNLQLDMTKLGFEEQQLKDFMEAIDRPYGMV
LVTGPTGSGKTTTLYSALSKLNEVAWNISTAEDPVEFNFFGINQVQMHEDIGLNFAAALRSFLRQDPDIIMVGEIRDFET
AEIGVKAALTGHLVLSTLHTNDAPGTVSRLLNMGIEPFLVTASLNAIVAQRLCRRLCPECRKAVAPDEQALLDAGFPAEE
IGTFQVYEPGGCKACNDRGYKGRVAVYEVMPLWDGLKELVIQGCSAAELKQEAIRLGFRTLRMSALNKVKTGMTSLAEAV
GNTAPDKF

Sequences:

>Translated_568_residues
MSGRLGELLVRENLISLQQLQKAQDEQRKTGGRIGSLLVKQGAIAESDLTGFLSKQYGVPAISLKDFDIDDEVLKLVPKA
AAEKHQVIPVNRAGSSLIVAMSDPSNIFAIDDVKFLTGYNVEVVVASEQAIREAIEKYYAEKGPDLDEVMQGFDDSEVAI
VEADGDDMNVVDLEKSAEEAPVVKLVNLILLDAIKKGASDIHVEPYEKDFRVRFRIDGVLYEVMKPPMKLRNAIISRLKI
MSELDISERRLPQDGRIKLKLGKGKEMDFRVSICPTLFGEKVVMRLLDKSNLQLDMTKLGFEEQQLKDFMEAIDRPYGMV
LVTGPTGSGKTTTLYSALSKLNEVAWNISTAEDPVEFNFFGINQVQMHEDIGLNFAAALRSFLRQDPDIIMVGEIRDFET
AEIGVKAALTGHLVLSTLHTNDAPGTVSRLLNMGIEPFLVTASLNAIVAQRLCRRLCPECRKAVAPDEQALLDAGFPAEE
IGTFQVYEPGGCKACNDRGYKGRVAVYEVMPLWDGLKELVIQGCSAAELKQEAIRLGFRTLRMSALNKVKTGMTSLAEAV
GNTAPDKF
>Mature_567_residues
SGRLGELLVRENLISLQQLQKAQDEQRKTGGRIGSLLVKQGAIAESDLTGFLSKQYGVPAISLKDFDIDDEVLKLVPKAA
AEKHQVIPVNRAGSSLIVAMSDPSNIFAIDDVKFLTGYNVEVVVASEQAIREAIEKYYAEKGPDLDEVMQGFDDSEVAIV
EADGDDMNVVDLEKSAEEAPVVKLVNLILLDAIKKGASDIHVEPYEKDFRVRFRIDGVLYEVMKPPMKLRNAIISRLKIM
SELDISERRLPQDGRIKLKLGKGKEMDFRVSICPTLFGEKVVMRLLDKSNLQLDMTKLGFEEQQLKDFMEAIDRPYGMVL
VTGPTGSGKTTTLYSALSKLNEVAWNISTAEDPVEFNFFGINQVQMHEDIGLNFAAALRSFLRQDPDIIMVGEIRDFETA
EIGVKAALTGHLVLSTLHTNDAPGTVSRLLNMGIEPFLVTASLNAIVAQRLCRRLCPECRKAVAPDEQALLDAGFPAEEI
GTFQVYEPGGCKACNDRGYKGRVAVYEVMPLWDGLKELVIQGCSAAELKQEAIRLGFRTLRMSALNKVKTGMTSLAEAVG
NTAPDKF

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=391, Percent_Identity=47.8260869565217, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1786296, Length=383, Percent_Identity=43.6031331592689, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI87082188, Length=272, Percent_Identity=32.7205882352941, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62620; Mature: 62489

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRLGELLVRENLISLQQLQKAQDEQRKTGGRIGSLLVKQGAIAESDLTGFLSKQYGVP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCC
AISLKDFDIDDEVLKLVPKAAAEKHQVIPVNRAGSSLIVAMSDPSNIFAIDDVKFLTGYN
EEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCEEEECCEEEECCCC
VEVVVASEQAIREAIEKYYAEKGPDLDEVMQGFDDSEVAIVEADGDDMNVVDLEKSAEEA
EEEEEECHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCCCEEEECCCCCCCC
PVVKLVNLILLDAIKKGASDIHVEPYEKDFRVRFRIDGVLYEVMKPPMKLRNAIISRLKI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHH
MSELDISERRLPQDGRIKLKLGKGKEMDFRVSICPTLFGEKVVMRLLDKSNLQLDMTKLG
HHHCCCHHHCCCCCCCEEEEECCCCCCEEEEEECHHHHHHHHHHHHHCCCCCEEEHHHCC
FEEQQLKDFMEAIDRPYGMVLVTGPTGSGKTTTLYSALSKLNEVAWNISTAEDPVEFNFF
CCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
GINQVQMHEDIGLNFAAALRSFLRQDPDIIMVGEIRDFETAEIGVKAALTGHLVLSTLHT
ECCEEEEHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHCHHHHHHHHHHEEEECC
NDAPGTVSRLLNMGIEPFLVTASLNAIVAQRLCRRLCPECRKAVAPDEQALLDAGFPAEE
CCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHCCCCCHHH
IGTFQVYEPGGCKACNDRGYKGRVAVYEVMPLWDGLKELVIQGCSAAELKQEAIRLGFRT
CCEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LRMSALNKVKTGMTSLAEAVGNTAPDKF
HHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SGRLGELLVRENLISLQQLQKAQDEQRKTGGRIGSLLVKQGAIAESDLTGFLSKQYGVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCC
AISLKDFDIDDEVLKLVPKAAAEKHQVIPVNRAGSSLIVAMSDPSNIFAIDDVKFLTGYN
EEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCEEEECCEEEECCCC
VEVVVASEQAIREAIEKYYAEKGPDLDEVMQGFDDSEVAIVEADGDDMNVVDLEKSAEEA
EEEEEECHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCCCEEEECCCCCCCC
PVVKLVNLILLDAIKKGASDIHVEPYEKDFRVRFRIDGVLYEVMKPPMKLRNAIISRLKI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHH
MSELDISERRLPQDGRIKLKLGKGKEMDFRVSICPTLFGEKVVMRLLDKSNLQLDMTKLG
HHHCCCHHHCCCCCCCEEEEECCCCCCEEEEEECHHHHHHHHHHHHHCCCCCEEEHHHCC
FEEQQLKDFMEAIDRPYGMVLVTGPTGSGKTTTLYSALSKLNEVAWNISTAEDPVEFNFF
CCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
GINQVQMHEDIGLNFAAALRSFLRQDPDIIMVGEIRDFETAEIGVKAALTGHLVLSTLHT
ECCEEEEHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHCHHHHHHHHHHEEEECC
NDAPGTVSRLLNMGIEPFLVTASLNAIVAQRLCRRLCPECRKAVAPDEQALLDAGFPAEE
CCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHCCCCCHHH
IGTFQVYEPGGCKACNDRGYKGRVAVYEVMPLWDGLKELVIQGCSAAELKQEAIRLGFRT
CCEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LRMSALNKVKTGMTSLAEAVGNTAPDKF
HHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]