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The map label for this gene is pilT [H]

Identifier: 220915776

GI number: 220915776

Start: 731557

End: 732657

Strand: Direct

Name: pilT [H]

Synonym: A2cp1_0657

Alternate gene names: 220915776

Gene position: 731557-732657 (Clockwise)

Preceding gene: 220915775

Following gene: 220915777

Centisome position: 14.55

GC content: 67.57

Gene sequence:

>1101_bases
TTGGCGAACCTGCACCAGCTCCTGAAGGCGATGGTCGAGAAGGGGGCCTCCGACCTCCACGTCACCACCGGCTCCCCGCC
CCAGCTCCGCATCGACGGCAAGCTGGTGCCGCTGAAGACCCCGCCGCTCTCGCCCGTCGAGACGAAGCAGCTCTGCTACT
CGATCCTCACCGACGCGCAGAAGCACAAGTTCGAGGAGGAGAGCGAGCTCGACCTCTCCTTCGGCGTGAAGGGGCTCTCG
CGCTTCCGCGCGAACGTCTTCATGCAGCGCGGCGCGGTGGCCGGCGCGTTCCGCACCATCCCGTTCAAGATCCTCACCTT
CCAGGAGCTGGGCCTGCCGCCCATCGTGGCCGAGCTGGCCAAGAAGCCGCGCGGCCTGGTGCTCGTCACCGGCCCCACCG
GCTCCGGCAAGTCCACCACGCTCGCGTCGATCATCGACAAGATCAACACGGACCGCCACGAGCACATCATCACGATCGAG
GATCCGATCGAGTACCTGCACCCGCACAAGAACTGCGTGGTGAACCAGCGCGAGGTCGGGGCGGACACCGCCTCGTTCAA
GAAGGCGCTGAAGTACATCCTCCGCCAGGATCCCGACGTCGTGCTCGTCGGCGAGATGCGCGATCTCGAGACCATCGAGG
CGGCGCTGGTGATCTCCGAGACGGGCCACCTCGCCTTCGCGACGCTGCACACCAACAGCGCGGTCCAGACGATCAACCGC
ATCCTGGACGTGTTCCCGCCCTACCAGCAGCCGCAGGTCCGCGCGCAGCTCTCCTTCGTGCTGGAGGGCGTGCTCACCCA
GAACCTGCTGCCCAGGGCCGGCGGCCCCGGCCGCGTGCTCATCATCGAGGTGATGGTCCCGAACCCCGCGATCCGGAACC
TCATCCGCGAGGACAAGGTGCACCAGGTCTACTCGCAGATGCAGGTCGGCCAGGCCAAGTTCGGCATGCAGACCTTCAAC
CAGAGCCTGGCGATGGCGTTCGCGAAGCGCCTCATCACGCTGGAGGAGGCGCTCGGCCGGTCCTCCGACGCGGAGGAGCT
GAAGAACATCATCGCGAGCGGCGCCGCGCTGCGCGGGCCGCCGCAAGGTGCGGCGCGGTAG

Upstream 100 bases:

>100_bases
CTGCGCATGAGCGCGCTCAACAAGGTGAAGACCGGCATGACCAGCCTCGCCGAGGCGGTCGGCAACACCGCGCCGGACAA
GTTCTAGGAGACGGCCAACC

Downstream 100 bases:

>100_bases
CAGCCCCGGATTCGACCCGATCCCACCCCCGCGCTAGGATGCGGGAGCCGTCGCTTCGTGAGGAGACCATGGCCCAGGCC
GCAGCCGCGAACGTGAAGAC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MANLHQLLKAMVEKGASDLHVTTGSPPQLRIDGKLVPLKTPPLSPVETKQLCYSILTDAQKHKFEEESELDLSFGVKGLS
RFRANVFMQRGAVAGAFRTIPFKILTFQELGLPPIVAELAKKPRGLVLVTGPTGSGKSTTLASIIDKINTDRHEHIITIE
DPIEYLHPHKNCVVNQREVGADTASFKKALKYILRQDPDVVLVGEMRDLETIEAALVISETGHLAFATLHTNSAVQTINR
ILDVFPPYQQPQVRAQLSFVLEGVLTQNLLPRAGGPGRVLIIEVMVPNPAIRNLIREDKVHQVYSQMQVGQAKFGMQTFN
QSLAMAFAKRLITLEEALGRSSDAEELKNIIASGAALRGPPQGAAR

Sequences:

>Translated_366_residues
MANLHQLLKAMVEKGASDLHVTTGSPPQLRIDGKLVPLKTPPLSPVETKQLCYSILTDAQKHKFEEESELDLSFGVKGLS
RFRANVFMQRGAVAGAFRTIPFKILTFQELGLPPIVAELAKKPRGLVLVTGPTGSGKSTTLASIIDKINTDRHEHIITIE
DPIEYLHPHKNCVVNQREVGADTASFKKALKYILRQDPDVVLVGEMRDLETIEAALVISETGHLAFATLHTNSAVQTINR
ILDVFPPYQQPQVRAQLSFVLEGVLTQNLLPRAGGPGRVLIIEVMVPNPAIRNLIREDKVHQVYSQMQVGQAKFGMQTFN
QSLAMAFAKRLITLEEALGRSSDAEELKNIIASGAALRGPPQGAAR
>Mature_365_residues
ANLHQLLKAMVEKGASDLHVTTGSPPQLRIDGKLVPLKTPPLSPVETKQLCYSILTDAQKHKFEEESELDLSFGVKGLSR
FRANVFMQRGAVAGAFRTIPFKILTFQELGLPPIVAELAKKPRGLVLVTGPTGSGKSTTLASIIDKINTDRHEHIITIED
PIEYLHPHKNCVVNQREVGADTASFKKALKYILRQDPDVVLVGEMRDLETIEAALVISETGHLAFATLHTNSAVQTINRI
LDVFPPYQQPQVRAQLSFVLEGVLTQNLLPRAGGPGRVLIIEVMVPNPAIRNLIREDKVHQVYSQMQVGQAKFGMQTFNQ
SLAMAFAKRLITLEEALGRSSDAEELKNIIASGAALRGPPQGAAR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=322, Percent_Identity=40.6832298136646, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1786296, Length=383, Percent_Identity=30.8093994778068, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1789723, Length=281, Percent_Identity=33.0960854092527, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40107; Mature: 39976

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLHQLLKAMVEKGASDLHVTTGSPPQLRIDGKLVPLKTPPLSPVETKQLCYSILTDAQ
CCCHHHHHHHHHHCCCCCEEEECCCCCEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHH
KHKFEEESELDLSFGVKGLSRFRANVFMQRGAVAGAFRTIPFKILTFQELGLPPIVAELA
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEEHHHCCCCHHHHHHH
KKPRGLVLVTGPTGSGKSTTLASIIDKINTDRHEHIITIEDPIEYLHPHKNCVVNQREVG
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCCCHHHCC
ADTASFKKALKYILRQDPDVVLVGEMRDLETIEAALVISETGHLAFATLHTNSAVQTINR
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEEEECCCCEEEEEEECCHHHHHHHH
ILDVFPPYQQPQVRAQLSFVLEGVLTQNLLPRAGGPGRVLIIEVMVPNPAIRNLIREDKV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHH
HQVYSQMQVGQAKFGMQTFNQSLAMAFAKRLITLEEALGRSSDAEELKNIIASGAALRGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
PQGAAR
CCCCCC
>Mature Secondary Structure 
ANLHQLLKAMVEKGASDLHVTTGSPPQLRIDGKLVPLKTPPLSPVETKQLCYSILTDAQ
CCHHHHHHHHHHCCCCCEEEECCCCCEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHH
KHKFEEESELDLSFGVKGLSRFRANVFMQRGAVAGAFRTIPFKILTFQELGLPPIVAELA
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEEHHHCCCCHHHHHHH
KKPRGLVLVTGPTGSGKSTTLASIIDKINTDRHEHIITIEDPIEYLHPHKNCVVNQREVG
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCCCHHHCC
ADTASFKKALKYILRQDPDVVLVGEMRDLETIEAALVISETGHLAFATLHTNSAVQTINR
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEEEECCCCEEEEEEECCHHHHHHHH
ILDVFPPYQQPQVRAQLSFVLEGVLTQNLLPRAGGPGRVLIIEVMVPNPAIRNLIREDKV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHH
HQVYSQMQVGQAKFGMQTFNQSLAMAFAKRLITLEEALGRSSDAEELKNIIASGAALRGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
PQGAAR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]