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The map label for this gene is punA [H]

Identifier: 220915771

GI number: 220915771

Start: 726950

End: 727798

Strand: Direct

Name: punA [H]

Synonym: A2cp1_0652

Alternate gene names: 220915771

Gene position: 726950-727798 (Clockwise)

Preceding gene: 220915770

Following gene: 220915772

Centisome position: 14.45

GC content: 75.74

Gene sequence:

>849_bases
ATGACCCGGCGCGCCGATCTCCCCACCCGGCTCTCCTACGCGCTCGCCTGGGTGCGCGGCCAGGCCGAGGCCGCGCCCGC
CGCCGGCGTCATCCTGGGCTCCGGCCTGTCCGCGTTCGCCGAGCGCCTGCAGCACCCGGTGGTGATCCCCTACGAGCGCA
TCCCGGGCTTTCCGGTCGCCAAGGTGGTGGGCCACCCCGGGCGGCTGGTGCTGGGCGAGCTGGACGCGGAGGGTGGCCCG
GTGCCCATCGCGGCCATGCAGGGGCGGGTGCACGTGTACGAGGGCTGGTCGCCGGAGGACGTCGCCTTCGGCGCCCGCGT
GCTGTGCGGCCTCGGCATTCGCGCGCTGCTCGTCACCAACGCGGCGGGCGGGGTGAACCCCGCGCTCGGCCCCGGCGACC
TGGTGCGCATCACGGACCACCTGAACCTCTCCGGCCAGAACCCGCTCGTCGGCGAGAACCTCGACCGGCTCGGGCCGCGC
TTCCCGGACATGTCCGAGGCCTACGACGAGCGGCTGGGCGCGCTGCTCGACGCGACCGCGGCGGAGCTCGGGCTCCCGCT
CGCCCGCGGCGTGTACGCCTGCATGCTGGGGCCCTCGTACGAGACGCCCGCCGAGGTGCGCATGCTGAGGGCGCTCGGCG
CGGACCTGGTGGGCATGTCCACCGTCCCGGAGGTGATCGTGGCGCGCCACATGGGCGTGCCCGTGGCCGGGCTCTCGGTG
GTGACGAACCACGCGGCCGGGCTGGTGCGGAAGCCCCTCACGCACGCGGAGGTGGCCGCGACCGCGGACCGCGTCCGGGA
CCAGCTCGGGGCGCTGGTGTCGGCGTTTCTCGCACGGGCCGGGCGTTGA

Upstream 100 bases:

>100_bases
GCAGCGAGCCGCGCGAGCGCATCGCCGCCCGGCTCGCGGGCGCGTACCGCATCGGCCCGGCCGCGCCCCCCGCCGCGCCC
CTGTTCCTCGAGAGGCTGAC

Downstream 100 bases:

>100_bases
TGGCGCCCCCCGGGCGCTGCTAGCCTCGCGATCCCGAGGTGGCGCGCATGCGTGAGCCGAACACGGCAGGTGGACCGGCG
GTGACGCCGGAGGCGGATCG

Product: inosine guanosine and xanthosine phosphorylase family protein

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTRRADLPTRLSYALAWVRGQAEAAPAAGVILGSGLSAFAERLQHPVVIPYERIPGFPVAKVVGHPGRLVLGELDAEGGP
VPIAAMQGRVHVYEGWSPEDVAFGARVLCGLGIRALLVTNAAGGVNPALGPGDLVRITDHLNLSGQNPLVGENLDRLGPR
FPDMSEAYDERLGALLDATAAELGLPLARGVYACMLGPSYETPAEVRMLRALGADLVGMSTVPEVIVARHMGVPVAGLSV
VTNHAAGLVRKPLTHAEVAATADRVRDQLGALVSAFLARAGR

Sequences:

>Translated_282_residues
MTRRADLPTRLSYALAWVRGQAEAAPAAGVILGSGLSAFAERLQHPVVIPYERIPGFPVAKVVGHPGRLVLGELDAEGGP
VPIAAMQGRVHVYEGWSPEDVAFGARVLCGLGIRALLVTNAAGGVNPALGPGDLVRITDHLNLSGQNPLVGENLDRLGPR
FPDMSEAYDERLGALLDATAAELGLPLARGVYACMLGPSYETPAEVRMLRALGADLVGMSTVPEVIVARHMGVPVAGLSV
VTNHAAGLVRKPLTHAEVAATADRVRDQLGALVSAFLARAGR
>Mature_281_residues
TRRADLPTRLSYALAWVRGQAEAAPAAGVILGSGLSAFAERLQHPVVIPYERIPGFPVAKVVGHPGRLVLGELDAEGGPV
PIAAMQGRVHVYEGWSPEDVAFGARVLCGLGIRALLVTNAAGGVNPALGPGDLVRITDHLNLSGQNPLVGENLDRLGPRF
PDMSEAYDERLGALLDATAAELGLPLARGVYACMLGPSYETPAEVRMLRALGADLVGMSTVPEVIVARHMGVPVAGLSVV
TNHAAGLVRKPLTHAEVAATADRVRDQLGALVSAFLARAGR

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=269, Percent_Identity=43.4944237918216, Blast_Score=204, Evalue=6e-53,
Organism=Homo sapiens, GI47132622, Length=260, Percent_Identity=26.9230769230769, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1788746, Length=257, Percent_Identity=43.579766536965, Blast_Score=211, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17541190, Length=268, Percent_Identity=38.8059701492537, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323238, Length=275, Percent_Identity=42.9090909090909, Blast_Score=187, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24656090, Length=261, Percent_Identity=41.3793103448276, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24656093, Length=261, Percent_Identity=41.3793103448276, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI45552885, Length=261, Percent_Identity=41.3793103448276, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI45552887, Length=261, Percent_Identity=42.5287356321839, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24762376, Length=272, Percent_Identity=33.8235294117647, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI221459247, Length=247, Percent_Identity=28.3400809716599, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29410; Mature: 29279

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRADLPTRLSYALAWVRGQAEAAPAAGVILGSGLSAFAERLQHPVVIPYERIPGFPVA
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHCCCEEEEHHHCCCCCHH
KVVGHPGRLVLGELDAEGGPVPIAAMQGRVHVYEGWSPEDVAFGARVLCGLGIRALLVTN
HHHCCCCCEEEEEECCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHHHHHCHHHHHEEC
AAGGVNPALGPGDLVRITDHLNLSGQNPLVGENLDRLGPRFPDMSEAYDERLGALLDATA
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
AELGLPLARGVYACMLGPSYETPAEVRMLRALGADLVGMSTVPEVIVARHMGVPVAGLSV
HHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHCHHHHHHHHHHHHCCCCHHHHHH
VTNHAAGLVRKPLTHAEVAATADRVRDQLGALVSAFLARAGR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRRADLPTRLSYALAWVRGQAEAAPAAGVILGSGLSAFAERLQHPVVIPYERIPGFPVA
CCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHCCCEEEEHHHCCCCCHH
KVVGHPGRLVLGELDAEGGPVPIAAMQGRVHVYEGWSPEDVAFGARVLCGLGIRALLVTN
HHHCCCCCEEEEEECCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHHHHHCHHHHHEEC
AAGGVNPALGPGDLVRITDHLNLSGQNPLVGENLDRLGPRFPDMSEAYDERLGALLDATA
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
AELGLPLARGVYACMLGPSYETPAEVRMLRALGADLVGMSTVPEVIVARHMGVPVAGLSV
HHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHCHHHHHHHHHHHHCCCCHHHHHH
VTNHAAGLVRKPLTHAEVAATADRVRDQLGALVSAFLARAGR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]