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The map label for this gene is sphR [H]

Identifier: 220915559

GI number: 220915559

Start: 498127

End: 498819

Strand: Direct

Name: sphR [H]

Synonym: A2cp1_0440

Alternate gene names: 220915559

Gene position: 498127-498819 (Clockwise)

Preceding gene: 220915558

Following gene: 220915561

Centisome position: 9.9

GC content: 70.42

Gene sequence:

>693_bases
ATGGCTGAGCGGATCCTGGTGATCGAGGACGACCCCTCCATCCTGCGGGGCCTGCAGCTCAACCTCGGGATGGAGGGCTA
CACCGTCCGGTCCGCGACGGACGGCGAGACCGGGCTGCAGCTGGCGCGCACCGAGCAGCCGGATCTCGTGGTGGTGGACG
TGATGTTGCCACGCATGGGCGGGCTGGAGGTGATCCGCGAGATCCGCAAGGAGGATCCCGAGCTCCCCATCCTCATCCTG
TCGGCGAAGAACCAGGAGACCGACAAGGTGGCCGGGCTGCAGCTCGGCGCCGACGACTACCTGGGCAAGCCGTTCGGGCT
CAAGGAGCTGCTGGCGCGCATCGACGCGCTGCTGCGCCGGCGCCGCGCGCGCGGCGAGGGCGTGCAGCCGAAGGCGATCC
GCAGGGTGGGGGGCATCGAGCTCGATCTGGACGCGCGGCGCGCGCTGGTGGACGGCCGCGCCCTCGAGCTGACCAGCCGC
GAGTTCGACCTGCTCGCGTTCTTCGTCACCCACCCGGACCGCGTCTACTCGCGCGAGCAGCTCATGGAGGCGGTGTGGGG
CTCGCGCTACTTCGGCACGGCCCGCACCGTGGACAACTTCGTGGCCCGGCTCCGCGCCCACATCGGCGACGACGCCGAGC
AGCCGCGCCACCTCGAGACGGTCCGCGGCATCGGCTACAGATTCAATGTTTGA

Upstream 100 bases:

>100_bases
AAGCACATCGTGGTCGGGCACGGCGGCAAGGTGTCGGTGGAGAGCGAGCCCGGGTGCGGCGCGACGTTCCGCATCGCGCT
GCCGGTGGCCGGAGGGGCCC

Downstream 100 bases:

>100_bases
GGGCAGCCGGCTTCAGACGGCCAGCGCGGCCCGCACCGCCGCGACCAGGGCCTCGTCCTCGGCCGGCAGCACGGTCCGCG
GGTCGAGCACCACGCGATCC

Product: winged helix family two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MAERILVIEDDPSILRGLQLNLGMEGYTVRSATDGETGLQLARTEQPDLVVVDVMLPRMGGLEVIREIRKEDPELPILIL
SAKNQETDKVAGLQLGADDYLGKPFGLKELLARIDALLRRRRARGEGVQPKAIRRVGGIELDLDARRALVDGRALELTSR
EFDLLAFFVTHPDRVYSREQLMEAVWGSRYFGTARTVDNFVARLRAHIGDDAEQPRHLETVRGIGYRFNV

Sequences:

>Translated_230_residues
MAERILVIEDDPSILRGLQLNLGMEGYTVRSATDGETGLQLARTEQPDLVVVDVMLPRMGGLEVIREIRKEDPELPILIL
SAKNQETDKVAGLQLGADDYLGKPFGLKELLARIDALLRRRRARGEGVQPKAIRRVGGIELDLDARRALVDGRALELTSR
EFDLLAFFVTHPDRVYSREQLMEAVWGSRYFGTARTVDNFVARLRAHIGDDAEQPRHLETVRGIGYRFNV
>Mature_229_residues
AERILVIEDDPSILRGLQLNLGMEGYTVRSATDGETGLQLARTEQPDLVVVDVMLPRMGGLEVIREIRKEDPELPILILS
AKNQETDKVAGLQLGADDYLGKPFGLKELLARIDALLRRRRARGEGVQPKAIRRVGGIELDLDARRALVDGRALELTSRE
FDLLAFFVTHPDRVYSREQLMEAVWGSRYFGTARTVDNFVARLRAHIGDDAEQPRHLETVRGIGYRFNV

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=235, Percent_Identity=39.5744680851064, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1789809, Length=227, Percent_Identity=37.8854625550661, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1786784, Length=227, Percent_Identity=37.8854625550661, Blast_Score=148, Evalue=2e-37,
Organism=Escherichia coli, GI1786911, Length=224, Percent_Identity=37.0535714285714, Blast_Score=140, Evalue=5e-35,
Organism=Escherichia coli, GI1790860, Length=224, Percent_Identity=36.6071428571429, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788394, Length=227, Percent_Identity=35.6828193832599, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI87082012, Length=225, Percent_Identity=32.4444444444444, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1790552, Length=223, Percent_Identity=37.219730941704, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1789402, Length=223, Percent_Identity=37.219730941704, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1790863, Length=229, Percent_Identity=33.6244541484716, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI2367329, Length=231, Percent_Identity=36.7965367965368, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787375, Length=228, Percent_Identity=32.4561403508772, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=32.1739130434783, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI145693140, Length=231, Percent_Identity=26.8398268398268, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788550, Length=107, Percent_Identity=32.7102803738318, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790299, Length=133, Percent_Identity=30.0751879699248, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=36.8421052631579, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1788905, Length=117, Percent_Identity=36.7521367521368, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788713, Length=113, Percent_Identity=34.5132743362832, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787487, Length=104, Percent_Identity=33.6538461538462, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787473, Length=177, Percent_Identity=31.638418079096, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25873; Mature: 25742

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERILVIEDDPSILRGLQLNLGMEGYTVRSATDGETGLQLARTEQPDLVVVDVMLPRMG
CCCEEEEEECCHHHHHCEEHCCCCCCEEEECCCCCCCCCEEECCCCCCEEEEEEECCCCC
GLEVIREIRKEDPELPILILSAKNQETDKVAGLQLGADDYLGKPFGLKELLARIDALLRR
CHHHHHHHHHCCCCCCEEEEECCCCCCHHHHCEEECCHHHCCCCCCHHHHHHHHHHHHHH
RRARGEGVQPKAIRRVGGIELDLDARRALVDGRALELTSREFDLLAFFVTHPDRVYSREQ
HHHCCCCCCHHHHHHHCCEEECHHHHHHHHCCCEEEECCCCEEEEEEEECCCHHHHHHHH
LMEAVWGSRYFGTARTVDNFVARLRAHIGDDAEQPRHLETVRGIGYRFNV
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEECC
>Mature Secondary Structure 
AERILVIEDDPSILRGLQLNLGMEGYTVRSATDGETGLQLARTEQPDLVVVDVMLPRMG
CCEEEEEECCHHHHHCEEHCCCCCCEEEECCCCCCCCCEEECCCCCCEEEEEEECCCCC
GLEVIREIRKEDPELPILILSAKNQETDKVAGLQLGADDYLGKPFGLKELLARIDALLRR
CHHHHHHHHHCCCCCCEEEEECCCCCCHHHHCEEECCHHHCCCCCCHHHHHHHHHHHHHH
RRARGEGVQPKAIRRVGGIELDLDARRALVDGRALELTSREFDLLAFFVTHPDRVYSREQ
HHHCCCCCCHHHHHHHCCEEECHHHHHHHHCCCEEEECCCCEEEEEEEECCCHHHHHHHH
LMEAVWGSRYFGTARTVDNFVARLRAHIGDDAEQPRHLETVRGIGYRFNV
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]