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The map label for this gene is pcnB [H]

Identifier: 220915422

GI number: 220915422

Start: 336407

End: 338077

Strand: Direct

Name: pcnB [H]

Synonym: A2cp1_0301

Alternate gene names: 220915422

Gene position: 336407-338077 (Clockwise)

Preceding gene: 220915421

Following gene: 220915423

Centisome position: 6.69

GC content: 77.26

Gene sequence:

>1671_bases
ATGCACGAGCCATCCACGAAGAACGACCTTTTCCGTCCGGCGTACCCCGCAGAAGACCTCGAAGAGAACCGAGACCGCCG
CCCGCCACCGGGCCTGGCGCTCGATCCCCCCGAAGCCCCGCCGCCGGAGCACCCGCCCGAGATCCCCGTCGATCGCCTGG
ACGGCGACGCGCTGAAGGTGATCGCCCGGCTGCGGCACATGCACCACCAGGCGTACCTGGTGGGCGGCTGCGTCCGCGAT
CTGCTGCTCGGCGCCACCCCCAAGGACTTCGACGTCGCGACCGACGCCCACCCCGGCGAGGTCCGCGCCATCTTCCGCAA
CTGCCGCCTCATCGGCCGCCGCTTCCGCCTGGCGCACGTGTACTTCCGCGCCGGCAAGGTCATCGAGGTCGCCACCTTCC
GGAAGAACCCGACCGACGTCGCCGAGGACGTGGGCGAGGGCGAGGGCGACCTGCTCATCACCCGCGACAACGTGTTCGGC
ACCGCCGAGGAGGACTCGGTCCGCCGCGACTTCACGGTGAACGGGCTGTTCTACGACGTCGCCACCGGCGAGGTGATCGA
CTACGTGGGCGGGCGCGCCGACCTCGAGGCGCACCGGATCTCCACCATCGGCGATCCGGAGATCCGGATGCGCGAGGACC
CGGTGCGCGCGCTGCGCGCGGTGCGGTTCGCGGCGCGGCTCGGGTTCACCATCGCGCCGGACACGTTCGAGGCGATGCGG
CGGACCTCGGGCGAGCTTGCCCGCTGCGCGCCGGCCCGGGTGCTGGAGGAGACGTTCAAGCTGCTCCGCTGCGGGGGGTC
GGCCCGCGCGTTCGAGCTGCTGCGCGCCTGCGGCGCCCTGCCGGTGATCCTGCCCGCGCTCGGCGCGGCGCTGGAGACCT
GGGACGACGGGCGGCGCCGCGCGTTCTTCGCGCACCTCGCCGCGCTCGACCGGCTGGTCCGCTCCGGCGCGGAGGTCTCC
GAGGCGGTGCTGCTCGGCGCGCTGCTCATGCACCTCGGCGCCGACGCGCCGCGCGCCGGCCGTGCGGACGCTGCGCGCGG
GGACGAGGACGCCGGCGCTCCGCGCTGGGACGAGGCCGACGCGTTCCTCGCCTCGCTGGTGCAGACCGCCCGCCTCCCGC
GCAAGGTGGCCGAGCGCATCCGGCTGGCGCTGCACGCGCAGCGGCAGCTCGAGGAGCCGGGCAAGCGCCGGCGCCGCCGC
GGGCGCGGCCCCGCCGGCCAGACCTACTTCCAGGACGCGCTGCAGCTGCTCGAGATCCGCGTGCGCGCCACCGGCCGCGG
GGCCGAGGTGCTGGAGCGGTGGTCGGCCGAGGCGCCGCACCCCCACGCGCCGTCGGAGCGCCCGGGCCGGGCCGGCCCGC
GCGACGACCGCCACGAGCGCCGCGACCGCCGCGAGGGCTCCGCGACGCACGGCGCCGACCGCGACGAGGGCGCCGTGCGC
GAGGTGGTGATCCCGACCGAGGCGGCCGAGGCCGCGGACGAGGCGGATCCGGAGGTCCGCGAGGTCGACTCCTCGGCCGC
CGGCGAGGGCGGGGAGGGCGAGGCGTCGCAGGGCGGACGCCGCCGGCGCCGCCGCCGGGGCGGGAAGCGCCGCCGCCGCC
GCGGGGCGGGCGCGTCGGGGCCGGACGGGGGCGCGCAGGTGCCGCCCGCCGAGGCTGGCGGGCAGGGGTGA

Upstream 100 bases:

>100_bases
TCGCTAGAACCAGCCCCGAGGACGAGCCCGACGGTGCAGGCGCGCTTCCCTCCTACCCGGGATTTCCCCCGGCGAGGGCG
CCCCCCGATCCGGCGAACGC

Downstream 100 bases:

>100_bases
GCGCGCGCGGGAATCCTCCGCAACACCGCCGCCTTGACCGACCCCACCGCACCCGGTACAACCCGCCGACCATGGCGAGC
GAGAAAGCCTCCAACGCGAA

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MHEPSTKNDLFRPAYPAEDLEENRDRRPPPGLALDPPEAPPPEHPPEIPVDRLDGDALKVIARLRHMHHQAYLVGGCVRD
LLLGATPKDFDVATDAHPGEVRAIFRNCRLIGRRFRLAHVYFRAGKVIEVATFRKNPTDVAEDVGEGEGDLLITRDNVFG
TAEEDSVRRDFTVNGLFYDVATGEVIDYVGGRADLEAHRISTIGDPEIRMREDPVRALRAVRFAARLGFTIAPDTFEAMR
RTSGELARCAPARVLEETFKLLRCGGSARAFELLRACGALPVILPALGAALETWDDGRRRAFFAHLAALDRLVRSGAEVS
EAVLLGALLMHLGADAPRAGRADAARGDEDAGAPRWDEADAFLASLVQTARLPRKVAERIRLALHAQRQLEEPGKRRRRR
GRGPAGQTYFQDALQLLEIRVRATGRGAEVLERWSAEAPHPHAPSERPGRAGPRDDRHERRDRREGSATHGADRDEGAVR
EVVIPTEAAEAADEADPEVREVDSSAAGEGGEGEASQGGRRRRRRRGGKRRRRRGAGASGPDGGAQVPPAEAGGQG

Sequences:

>Translated_556_residues
MHEPSTKNDLFRPAYPAEDLEENRDRRPPPGLALDPPEAPPPEHPPEIPVDRLDGDALKVIARLRHMHHQAYLVGGCVRD
LLLGATPKDFDVATDAHPGEVRAIFRNCRLIGRRFRLAHVYFRAGKVIEVATFRKNPTDVAEDVGEGEGDLLITRDNVFG
TAEEDSVRRDFTVNGLFYDVATGEVIDYVGGRADLEAHRISTIGDPEIRMREDPVRALRAVRFAARLGFTIAPDTFEAMR
RTSGELARCAPARVLEETFKLLRCGGSARAFELLRACGALPVILPALGAALETWDDGRRRAFFAHLAALDRLVRSGAEVS
EAVLLGALLMHLGADAPRAGRADAARGDEDAGAPRWDEADAFLASLVQTARLPRKVAERIRLALHAQRQLEEPGKRRRRR
GRGPAGQTYFQDALQLLEIRVRATGRGAEVLERWSAEAPHPHAPSERPGRAGPRDDRHERRDRREGSATHGADRDEGAVR
EVVIPTEAAEAADEADPEVREVDSSAAGEGGEGEASQGGRRRRRRRGGKRRRRRGAGASGPDGGAQVPPAEAGGQG
>Mature_556_residues
MHEPSTKNDLFRPAYPAEDLEENRDRRPPPGLALDPPEAPPPEHPPEIPVDRLDGDALKVIARLRHMHHQAYLVGGCVRD
LLLGATPKDFDVATDAHPGEVRAIFRNCRLIGRRFRLAHVYFRAGKVIEVATFRKNPTDVAEDVGEGEGDLLITRDNVFG
TAEEDSVRRDFTVNGLFYDVATGEVIDYVGGRADLEAHRISTIGDPEIRMREDPVRALRAVRFAARLGFTIAPDTFEAMR
RTSGELARCAPARVLEETFKLLRCGGSARAFELLRACGALPVILPALGAALETWDDGRRRAFFAHLAALDRLVRSGAEVS
EAVLLGALLMHLGADAPRAGRADAARGDEDAGAPRWDEADAFLASLVQTARLPRKVAERIRLALHAQRQLEEPGKRRRRR
GRGPAGQTYFQDALQLLEIRVRATGRGAEVLERWSAEAPHPHAPSERPGRAGPRDDRHERRDRREGSATHGADRDEGAVR
EVVIPTEAAEAADEADPEVREVDSSAAGEGGEGEASQGGRRRRRRRGGKRRRRRGAGASGPDGGAQVPPAEAGGQG

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=242, Percent_Identity=30.9917355371901, Blast_Score=84, Evalue=5e-16,
Organism=Escherichia coli, GI87081691, Length=409, Percent_Identity=37.4083129584352, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1789436, Length=231, Percent_Identity=35.4978354978355, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321016, Length=223, Percent_Identity=29.1479820627803, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281360112, Length=236, Percent_Identity=29.2372881355932, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21357337, Length=236, Percent_Identity=29.2372881355932, Blast_Score=86, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 60780; Mature: 60780

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEPSTKNDLFRPAYPAEDLEENRDRRPPPGLALDPPEAPPPEHPPEIPVDRLDGDALKV
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
IARLRHMHHQAYLVGGCVRDLLLGATPKDFDVATDAHPGEVRAIFRNCRLIGRRFRLAHV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YFRAGKVIEVATFRKNPTDVAEDVGEGEGDLLITRDNVFGTAEEDSVRRDFTVNGLFYDV
HHHCCCEEEEEECCCCCHHHHHHCCCCCCCEEEEECCCCCCCCHHHHCCCEEECCEEEEC
ATGEVIDYVGGRADLEAHRISTIGDPEIRMREDPVRALRAVRFAARLGFTIAPDTFEAMR
CCCHHHHHCCCCCCCHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCEECCHHHHHHH
RTSGELARCAPARVLEETFKLLRCGGSARAFELLRACGALPVILPALGAALETWDDGRRR
HCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHH
AFFAHLAALDRLVRSGAEVSEAVLLGALLMHLGADAPRAGRADAARGDEDAGAPRWDEAD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHH
AFLASLVQTARLPRKVAERIRLALHAQRQLEEPGKRRRRRGRGPAGQTYFQDALQLLEIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VRATGRGAEVLERWSAEAPHPHAPSERPGRAGPRDDRHERRDRREGSATHGADRDEGAVR
HHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCE
EVVIPTEAAEAADEADPEVREVDSSAAGEGGEGEASQGGRRRRRRRGGKRRRRRGAGASG
EEECCCHHHHHHHCCCCHHHHHCHHCCCCCCCCCCCCHHHHHHHHHCHHHHHHCCCCCCC
PDGGAQVPPAEAGGQG
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHEPSTKNDLFRPAYPAEDLEENRDRRPPPGLALDPPEAPPPEHPPEIPVDRLDGDALKV
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
IARLRHMHHQAYLVGGCVRDLLLGATPKDFDVATDAHPGEVRAIFRNCRLIGRRFRLAHV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YFRAGKVIEVATFRKNPTDVAEDVGEGEGDLLITRDNVFGTAEEDSVRRDFTVNGLFYDV
HHHCCCEEEEEECCCCCHHHHHHCCCCCCCEEEEECCCCCCCCHHHHCCCEEECCEEEEC
ATGEVIDYVGGRADLEAHRISTIGDPEIRMREDPVRALRAVRFAARLGFTIAPDTFEAMR
CCCHHHHHCCCCCCCHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCEECCHHHHHHH
RTSGELARCAPARVLEETFKLLRCGGSARAFELLRACGALPVILPALGAALETWDDGRRR
HCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHH
AFFAHLAALDRLVRSGAEVSEAVLLGALLMHLGADAPRAGRADAARGDEDAGAPRWDEAD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHH
AFLASLVQTARLPRKVAERIRLALHAQRQLEEPGKRRRRRGRGPAGQTYFQDALQLLEIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VRATGRGAEVLERWSAEAPHPHAPSERPGRAGPRDDRHERRDRREGSATHGADRDEGAVR
HHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCE
EVVIPTEAAEAADEADPEVREVDSSAAGEGGEGEASQGGRRRRRRRGGKRRRRRGAGASG
EEECCCHHHHHHHCCCCHHHHHCHHCCCCCCCCCCCCHHHHHHHHHCHHHHHHCCCCCCC
PDGGAQVPPAEAGGQG
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]