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The map label for this gene is bhbA [H]

Identifier: 220915402

GI number: 220915402

Start: 315182

End: 316831

Strand: Direct

Name: bhbA [H]

Synonym: A2cp1_0281

Alternate gene names: 220915402

Gene position: 315182-316831 (Clockwise)

Preceding gene: 220915401

Following gene: 220915403

Centisome position: 6.27

GC content: 70.79

Gene sequence:

>1650_bases
ATGGCGGACGAGAAGAAGCAGCGGTGGCTCGAGGGCACCTACGCGAAGGCGGTGAAGAAGGGGCCCGAGCGGCGCCCGCG
CTTCGAGACGAGCAGCGGCATCCGCGAGGAGCCGGTGTACGGCGCGGCCGACGTGCGCCCCGGCCTCGACGAGCGGCTGG
GCCTGCCCGGCGAGTACCCGTTCACCCGCGGCGTGCAGCCCACCATGTACCGCGGCCGCTTCTGGACCATGCGGCAGTAC
GCCGGGTTCGGCACCGCCGAGGAGTCGAACAAGCGCTACCGCTACCTGCTCGAGTCCGGCCAGACCGGCCTCTCGGTCGC
GTTCGACCTGCCCACGCAGATGGGCCGCGACTCCGACCACCCCCGCGCCCGCGGCGAGGTGGGCAAGGTGGGCGTGGCCA
TCGACTCGATCCGCGACATGGAGGTGCTGTTCGACCGGATCCCGCTCGGCGAGGTCTCCACCTCGATGACCATCAACGCG
ACCGCCGGGATGCTGCTCGCGATGTACCAGGCGGTGGGCGAGAAGCAGGGGGCCGCGCCGGCGGTGCTGCAGGGCACCAT
CCAGAACGACATCCTGAAGGAGTACGCGGCGCGCGGGACGTACATCTACCCGCCGGAGCCGTCGCTGCGGATCATCACCG
ACATCTTCGCCTACACCGCGAAGGTGATGCCGCGCTGGAACCCCATCTCCATCTCCGGCTACCACATCCGCGAGGCCGGC
TCGACCGCGGTGCAGGAGGTGGCCTTCACGCTCGCCGACGGCATCCAGTACGTGGACGCGGCGGTGAAGGCGGGCCTCGA
CGTGGACGCGTTCGCGGGCCGGCTCTCCTTCTTCTTCAACGCGCACAACGACCTGCTGGAGGAGGTGGCGAAGTTCCGCG
CCGCGCGCCGGCTCTGGGCGCGCATCATGAAGGAGCGGTTCAAGGCGAAGGACCCGCGCTCGATGATGCTGCGGTTCCAC
ACCCAGACCGCCGGCTCCATGCTCACCGCGCAGCAGCCGGACAACAACATCGTGCGCGTCACCCTCCAGGCGCTGGCGGC
GGTGCTGGGCGGCACGCAGTCGCTGCACACCAACTCGCGCGACGAGGCGCTCGGGCTGCCCACCGAGGACTCGGTGCGCA
TCGCGCTCCGCACGCAGCAGATCATCGCGAACGAGTCGGGCGTCGCCGACGTCATCGACCCGCTCGGCGGCAGCTGGGCC
ATCGAGGCCATGACCGACGAGATCGAGGGGCGCGCCCAGGAGTACATCCGGAAGATCGACGAGCTGGGCGGCATGGTCCA
CGCGATCTCGAAGGGCTACGTGCAGCGCGAGATCCAGGAGGCCGCCTACGCCTGGCAGCGCCAGGTCGAGGCGAAGGACC
AGGTGGTCGTCGGCGTGAACGCGTTCAGGTCGGACGATCCGCCGGTGCCGGTGATGAAGGTCGATCCGGCGCTGGAGGCG
CAGCAGGTGGAGCGGCTGAAGGCGCTCCGCGCCGCCCGCAGCGCCTCGGCCGCGCGCGGCGCGCTCGACGCGGTGCGGGC
CGGCGCCCGCGGGACCGACAACCTCATGCCGCTCATCCTCGCCGCCGTGAAGGCCGAGTGCACGCTCGGGGAGATCTCCG
ACGCGCTGCGCGAGGTCTACGGCGAGTACCGGGAGACGGTGGTACTCTAG

Upstream 100 bases:

>100_bases
CAGCGCGTTGCTCTGCGCGGTGGAGTAGCGGCGCCGCGCCTCCCACTCCCAACCGGCGCCCCCCGTGACGGGCGCGCCGT
GCTCCACCCACGGTGAACAC

Downstream 100 bases:

>100_bases
CGCGCGGTGAGCGACGCACCCCCGAAGCCCCCCGGCGAGACGCCTCCCCCGCGGCCCCGGCCGGCGCCCCCGGCCGGCCC
GCCGCGCCTGGAGCGCGTGC

Product: methylmalonyl-CoA mutase, large subunit

Products: NA

Alternate protein names: MCM [H]

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MADEKKQRWLEGTYAKAVKKGPERRPRFETSSGIREEPVYGAADVRPGLDERLGLPGEYPFTRGVQPTMYRGRFWTMRQY
AGFGTAEESNKRYRYLLESGQTGLSVAFDLPTQMGRDSDHPRARGEVGKVGVAIDSIRDMEVLFDRIPLGEVSTSMTINA
TAGMLLAMYQAVGEKQGAAPAVLQGTIQNDILKEYAARGTYIYPPEPSLRIITDIFAYTAKVMPRWNPISISGYHIREAG
STAVQEVAFTLADGIQYVDAAVKAGLDVDAFAGRLSFFFNAHNDLLEEVAKFRAARRLWARIMKERFKAKDPRSMMLRFH
TQTAGSMLTAQQPDNNIVRVTLQALAAVLGGTQSLHTNSRDEALGLPTEDSVRIALRTQQIIANESGVADVIDPLGGSWA
IEAMTDEIEGRAQEYIRKIDELGGMVHAISKGYVQREIQEAAYAWQRQVEAKDQVVVGVNAFRSDDPPVPVMKVDPALEA
QQVERLKALRAARSASAARGALDAVRAGARGTDNLMPLILAAVKAECTLGEISDALREVYGEYRETVVL

Sequences:

>Translated_549_residues
MADEKKQRWLEGTYAKAVKKGPERRPRFETSSGIREEPVYGAADVRPGLDERLGLPGEYPFTRGVQPTMYRGRFWTMRQY
AGFGTAEESNKRYRYLLESGQTGLSVAFDLPTQMGRDSDHPRARGEVGKVGVAIDSIRDMEVLFDRIPLGEVSTSMTINA
TAGMLLAMYQAVGEKQGAAPAVLQGTIQNDILKEYAARGTYIYPPEPSLRIITDIFAYTAKVMPRWNPISISGYHIREAG
STAVQEVAFTLADGIQYVDAAVKAGLDVDAFAGRLSFFFNAHNDLLEEVAKFRAARRLWARIMKERFKAKDPRSMMLRFH
TQTAGSMLTAQQPDNNIVRVTLQALAAVLGGTQSLHTNSRDEALGLPTEDSVRIALRTQQIIANESGVADVIDPLGGSWA
IEAMTDEIEGRAQEYIRKIDELGGMVHAISKGYVQREIQEAAYAWQRQVEAKDQVVVGVNAFRSDDPPVPVMKVDPALEA
QQVERLKALRAARSASAARGALDAVRAGARGTDNLMPLILAAVKAECTLGEISDALREVYGEYRETVVL
>Mature_548_residues
ADEKKQRWLEGTYAKAVKKGPERRPRFETSSGIREEPVYGAADVRPGLDERLGLPGEYPFTRGVQPTMYRGRFWTMRQYA
GFGTAEESNKRYRYLLESGQTGLSVAFDLPTQMGRDSDHPRARGEVGKVGVAIDSIRDMEVLFDRIPLGEVSTSMTINAT
AGMLLAMYQAVGEKQGAAPAVLQGTIQNDILKEYAARGTYIYPPEPSLRIITDIFAYTAKVMPRWNPISISGYHIREAGS
TAVQEVAFTLADGIQYVDAAVKAGLDVDAFAGRLSFFFNAHNDLLEEVAKFRAARRLWARIMKERFKAKDPRSMMLRFHT
QTAGSMLTAQQPDNNIVRVTLQALAAVLGGTQSLHTNSRDEALGLPTEDSVRIALRTQQIIANESGVADVIDPLGGSWAI
EAMTDEIEGRAQEYIRKIDELGGMVHAISKGYVQREIQEAAYAWQRQVEAKDQVVVGVNAFRSDDPPVPVMKVDPALEAQ
QVERLKALRAARSASAARGALDAVRAGARGTDNLMPLILAAVKAECTLGEISDALREVYGEYRETVVL

Specific function: Catalyzes the interconversion of succinyl-CoA and methylmalonyl-CoA during the polyhydroxyalkanoate degradation pathway [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=520, Percent_Identity=49.2307692307692, Blast_Score=508, Evalue=1e-144,
Organism=Escherichia coli, GI1789284, Length=517, Percent_Identity=51.257253384913, Blast_Score=494, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI25144500, Length=540, Percent_Identity=53.1481481481481, Blast_Score=558, Evalue=1e-159,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 60479; Mature: 60347

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEKKQRWLEGTYAKAVKKGPERRPRFETSSGIREEPVYGAADVRPGLDERLGLPGEYP
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCC
FTRGVQPTMYRGRFWTMRQYAGFGTAEESNKRYRYLLESGQTGLSVAFDLPTQMGRDSDH
CCCCCCCCEECCCEEEHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCHHCCCCCCC
PRARGEVGKVGVAIDSIRDMEVLFDRIPLGEVSTSMTINATAGMLLAMYQAVGEKQGAAP
CCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCH
AVLQGTIQNDILKEYAARGTYIYPPEPSLRIITDIFAYTAKVMPRWNPISISGYHIREAG
HHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCEEECCEEECCCC
STAVQEVAFTLADGIQYVDAAVKAGLDVDAFAGRLSFFFNAHNDLLEEVAKFRAARRLWA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RIMKERFKAKDPRSMMLRFHTQTAGSMLTAQQPDNNIVRVTLQALAAVLGGTQSLHTNSR
HHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCHHCCCCCC
DEALGLPTEDSVRIALRTQQIIANESGVADVIDPLGGSWAIEAMTDEIEGRAQEYIRKID
CCCCCCCCCCCEEEEEEHHHHHHCCCCCHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
ELGGMVHAISKGYVQREIQEAAYAWQRQVEAKDQVVVGVNAFRSDDPPVPVMKVDPALEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCCCEEEECCCCCH
QQVERLKALRAARSASAARGALDAVRAGARGTDNLMPLILAAVKAECTLGEISDALREVY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
GEYRETVVL
HHHHHHCCC
>Mature Secondary Structure 
ADEKKQRWLEGTYAKAVKKGPERRPRFETSSGIREEPVYGAADVRPGLDERLGLPGEYP
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCC
FTRGVQPTMYRGRFWTMRQYAGFGTAEESNKRYRYLLESGQTGLSVAFDLPTQMGRDSDH
CCCCCCCCEECCCEEEHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCHHCCCCCCC
PRARGEVGKVGVAIDSIRDMEVLFDRIPLGEVSTSMTINATAGMLLAMYQAVGEKQGAAP
CCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCH
AVLQGTIQNDILKEYAARGTYIYPPEPSLRIITDIFAYTAKVMPRWNPISISGYHIREAG
HHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCEEECCEEECCCC
STAVQEVAFTLADGIQYVDAAVKAGLDVDAFAGRLSFFFNAHNDLLEEVAKFRAARRLWA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RIMKERFKAKDPRSMMLRFHTQTAGSMLTAQQPDNNIVRVTLQALAAVLGGTQSLHTNSR
HHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCHHCCCCCC
DEALGLPTEDSVRIALRTQQIIANESGVADVIDPLGGSWAIEAMTDEIEGRAQEYIRKID
CCCCCCCCCCCEEEEEEHHHHHHCCCCCHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
ELGGMVHAISKGYVQREIQEAAYAWQRQVEAKDQVVVGVNAFRSDDPPVPVMKVDPALEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCCCEEEECCCCCH
QQVERLKALRAARSASAARGALDAVRAGARGTDNLMPLILAAVKAECTLGEISDALREVY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
GEYRETVVL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9889346; 11481431 [H]