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The map label for this gene is pycA [H]

Identifier: 220915400

GI number: 220915400

Start: 313068

End: 314600

Strand: Direct

Name: pycA [H]

Synonym: A2cp1_0279

Alternate gene names: 220915400

Gene position: 313068-314600 (Clockwise)

Preceding gene: 220915398

Following gene: 220915401

Centisome position: 6.22

GC content: 74.95

Gene sequence:

>1533_bases
ATGAGCCCGACGAGACCCATCGAGAAGGTGCTGGTCGCGAACCGCGGCGAGATCGCGGTGCGCGTCATGCGCACCTGCCG
CGAGATGCGGATCCCCACCGTGGCGGTGTACTCCGAGGCGGACCGCGGCGCGCTCCACGTCCGCAAGGCCGACGAGGCGG
TGTTCATCGGCCCCGCGCCGGCGCGCGAGAGCTACCTCTCGATCGAGCGCATCCTGGACGCCTGCCGGCGCACCGGCGCC
GACGCGGTCCACCCCGGCTACGGCTTCCTCTCCGAGAACGCCGAGCTGGCCCGCGCGCTCGACCGCGCCGGGATCGCGCT
GGTCGGCCCGCCCGCCGCGGCGATGGACGCGATGGGCGTGAAGACCACCGCCCGGCGCAACATGGCCGCCGCCGGCGTGC
CGGTGGTGCCCGGCTCCGAGGAGCCGTTCGCCGAGGAGGCCGAGGCGCGCGCGTTCGCGGAGCGGATCGGCTTCCCGGTG
ATGATCAAGGCGGCGGCGGGCGGCGGCGGCAAGGGCATGAAGAAGTGCGACCGCGCCGAGGACTTCGCCGCGCTGTGGCA
GTCGGCGCGGCGCGAGGCGACGGCCGCGTTCGGCGACGACCGGCTCTACCTCGAGAAGTTCCTGGAGAAGCCGCGCCACG
TCGAGATCCAGATCTTCGCGGACCAGCACGGCAACTGCGTGTGGCTGGGCGAGCGCGAGTGCTCGGTGCAGCGGCGCCAG
CAGAAGGTGATCGAGGAGACGCCGAGCGCGGTGCTCGACGACCGGCTGCGGGAGGCCATGGGCGAGGTGGCCGTCCGCGC
CGCGCGCGCGGTGGGCTACGTGGGCGCCGGCACGGTCGAGCTGCTGGTGGACGCGCACCGCAACTTCTACTTCCTCGAGA
TGAACACCCGCCTGCAGGTGGAGCACCCGGTCACCGAGATGGTCACCGGGCTCGACCTGGTGCGCATGCAGCTCGAGGTG
GCGCGCGGCGCGCCGATCCTGGCGCAGGAGCAGGTGCAGCGACGCGGCCACGCCATCGAGGCGCGCGTCTACGCCGAGGA
CCCGGCCCGCGGGTTCCTGCCGCAGCCGGGCAAGATCACCTACCTGCGCGTGCCGGGCGGCCCGGGCATCCGCGACGACT
CCGGCGTGTACGCGGGCTGGGTGGTGCCGCAGTGGTACGACCCGATGATCTCGAAGCTGGTCGCCTGGGCGCCGACCCGC
CCGCAGGCCATCGACCGGCTCATCCGCGCGCTCGGCGAGTACGTGGTGCACGGGATCGGCACGAACCTGAGCTGGCTGGC
GGCCGCGCTCGACCACCCCGAGTTCCGCTCCGGCGACTACGACACCGGCTTCTGCGCGCGGAACGCGAAGGCGCTCATCC
GGCCGCCCGACCCGTCGCTGGAGCGGGTGGCGCTGATCGCGGCGGCGGTGGCCGCGTTCAGGCGCGAGCGCGACGCGGCC
GAGGCGCACGCGGCCCGGGCCGGGCAGGGGGCGGCGCGCTCCGGCTGGGCGCGCGCCGGCCGGCTGCGGGCGCTGCGCGG
GGGCGGGCGATGA

Upstream 100 bases:

>100_bases
GCGGCGGGCCCGCGCTTTACCGCGCAGGTCCGGGCGGCTATCGTGGCGGACCCCGGGGCGGCCGGGGGCCCCGCCGCGGC
CCGCCCCGACCGTTCCGCAC

Downstream 100 bases:

>100_bases
AGACCTACGTGGCGCTGCTCGACGGGGGCAAGCGCGAGGTGACGCTCGGGGTGACGCGGCTCGCCACCGGGCAGTACGAG
GTGCGCATCGGCGACGAGGT

Product: carbamoyl-phosphate synthase L chain ATP-binding

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MSPTRPIEKVLVANRGEIAVRVMRTCREMRIPTVAVYSEADRGALHVRKADEAVFIGPAPARESYLSIERILDACRRTGA
DAVHPGYGFLSENAELARALDRAGIALVGPPAAAMDAMGVKTTARRNMAAAGVPVVPGSEEPFAEEAEARAFAERIGFPV
MIKAAAGGGGKGMKKCDRAEDFAALWQSARREATAAFGDDRLYLEKFLEKPRHVEIQIFADQHGNCVWLGERECSVQRRQ
QKVIEETPSAVLDDRLREAMGEVAVRAARAVGYVGAGTVELLVDAHRNFYFLEMNTRLQVEHPVTEMVTGLDLVRMQLEV
ARGAPILAQEQVQRRGHAIEARVYAEDPARGFLPQPGKITYLRVPGGPGIRDDSGVYAGWVVPQWYDPMISKLVAWAPTR
PQAIDRLIRALGEYVVHGIGTNLSWLAAALDHPEFRSGDYDTGFCARNAKALIRPPDPSLERVALIAAAVAAFRRERDAA
EAHAARAGQGAARSGWARAGRLRALRGGGR

Sequences:

>Translated_510_residues
MSPTRPIEKVLVANRGEIAVRVMRTCREMRIPTVAVYSEADRGALHVRKADEAVFIGPAPARESYLSIERILDACRRTGA
DAVHPGYGFLSENAELARALDRAGIALVGPPAAAMDAMGVKTTARRNMAAAGVPVVPGSEEPFAEEAEARAFAERIGFPV
MIKAAAGGGGKGMKKCDRAEDFAALWQSARREATAAFGDDRLYLEKFLEKPRHVEIQIFADQHGNCVWLGERECSVQRRQ
QKVIEETPSAVLDDRLREAMGEVAVRAARAVGYVGAGTVELLVDAHRNFYFLEMNTRLQVEHPVTEMVTGLDLVRMQLEV
ARGAPILAQEQVQRRGHAIEARVYAEDPARGFLPQPGKITYLRVPGGPGIRDDSGVYAGWVVPQWYDPMISKLVAWAPTR
PQAIDRLIRALGEYVVHGIGTNLSWLAAALDHPEFRSGDYDTGFCARNAKALIRPPDPSLERVALIAAAVAAFRRERDAA
EAHAARAGQGAARSGWARAGRLRALRGGGR
>Mature_509_residues
SPTRPIEKVLVANRGEIAVRVMRTCREMRIPTVAVYSEADRGALHVRKADEAVFIGPAPARESYLSIERILDACRRTGAD
AVHPGYGFLSENAELARALDRAGIALVGPPAAAMDAMGVKTTARRNMAAAGVPVVPGSEEPFAEEAEARAFAERIGFPVM
IKAAAGGGGKGMKKCDRAEDFAALWQSARREATAAFGDDRLYLEKFLEKPRHVEIQIFADQHGNCVWLGERECSVQRRQQ
KVIEETPSAVLDDRLREAMGEVAVRAARAVGYVGAGTVELLVDAHRNFYFLEMNTRLQVEHPVTEMVTGLDLVRMQLEVA
RGAPILAQEQVQRRGHAIEARVYAEDPARGFLPQPGKITYLRVPGGPGIRDDSGVYAGWVVPQWYDPMISKLVAWAPTRP
QAIDRLIRALGEYVVHGIGTNLSWLAAALDHPEFRSGDYDTGFCARNAKALIRPPDPSLERVALIAAAVAAFRRERDAAE
AHAARAGQGAARSGWARAGRLRALRGGGR

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG4770

COG function: function code I; Acetyl/propionyl-CoA carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI116805327, Length=451, Percent_Identity=51.8847006651885, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI189095269, Length=445, Percent_Identity=49.8876404494382, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI65506442, Length=445, Percent_Identity=49.8876404494382, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI295821183, Length=445, Percent_Identity=49.8876404494382, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI106049528, Length=459, Percent_Identity=46.8409586056645, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI106049295, Length=459, Percent_Identity=46.8409586056645, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI106049292, Length=459, Percent_Identity=46.8409586056645, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI38679977, Length=502, Percent_Identity=33.4661354581673, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI38679967, Length=502, Percent_Identity=33.4661354581673, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI38679974, Length=502, Percent_Identity=33.4661354581673, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI38679960, Length=502, Percent_Identity=33.4661354581673, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI38679971, Length=502, Percent_Identity=33.4661354581673, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI134142062, Length=502, Percent_Identity=33.2669322709163, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI18105007, Length=339, Percent_Identity=23.598820058997, Blast_Score=80, Evalue=7e-15,
Organism=Escherichia coli, GI1789654, Length=443, Percent_Identity=49.2099322799097, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17567343, Length=441, Percent_Identity=49.6598639455782, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71987519, Length=475, Percent_Identity=48.8421052631579, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17562816, Length=447, Percent_Identity=45.8612975391499, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71997168, Length=555, Percent_Identity=28.4684684684685, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI71997163, Length=555, Percent_Identity=28.4684684684685, Blast_Score=232, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI133931226, Length=534, Percent_Identity=30.5243445692884, Blast_Score=214, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI193204318, Length=204, Percent_Identity=25.9803921568627, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319695, Length=445, Percent_Identity=43.1460674157303, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6321376, Length=445, Percent_Identity=44.4943820224719, Blast_Score=357, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6319685, Length=437, Percent_Identity=42.3340961098398, Blast_Score=350, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6324343, Length=560, Percent_Identity=30.3571428571429, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6323863, Length=449, Percent_Identity=31.8485523385301, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24651757, Length=481, Percent_Identity=49.0644490644491, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24651759, Length=440, Percent_Identity=48.6363636363636, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24652212, Length=464, Percent_Identity=45.0431034482759, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24652210, Length=464, Percent_Identity=45.0431034482759, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24652214, Length=464, Percent_Identity=45.0431034482759, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI19921944, Length=464, Percent_Identity=45.0431034482759, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24652216, Length=464, Percent_Identity=45.0431034482759, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI281363050, Length=464, Percent_Identity=45.0431034482759, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24652224, Length=464, Percent_Identity=45.0431034482759, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24652222, Length=464, Percent_Identity=45.0431034482759, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24652220, Length=464, Percent_Identity=45.0431034482759, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24652218, Length=464, Percent_Identity=45.0431034482759, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI161076407, Length=497, Percent_Identity=32.7967806841046, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24586460, Length=497, Percent_Identity=32.7967806841046, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI161076409, Length=497, Percent_Identity=32.7967806841046, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24586458, Length=497, Percent_Identity=32.7967806841046, Blast_Score=252, Evalue=5e-67,
Organism=Drosophila melanogaster, GI45555749, Length=347, Percent_Identity=23.342939481268, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24642586, Length=347, Percent_Identity=23.342939481268, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 55562; Mature: 55431

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTRPIEKVLVANRGEIAVRVMRTCREMRIPTVAVYSEADRGALHVRKADEAVFIGPAP
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCEEEECCCC
ARESYLSIERILDACRRTGADAVHPGYGFLSENAELARALDRAGIALVGPPAAAMDAMGV
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCHHHHHHCCC
KTTARRNMAAAGVPVVPGSEEPFAEEAEARAFAERIGFPVMIKAAAGGGGKGMKKCDRAE
HHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHH
DFAALWQSARREATAAFGDDRLYLEKFLEKPRHVEIQIFADQHGNCVWLGERECSVQRRQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCHHHHHHHH
QKVIEETPSAVLDDRLREAMGEVAVRAARAVGYVGAGTVELLVDAHRNFYFLEMNTRLQV
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCEEEEEECCEEEE
EHPVTEMVTGLDLVRMQLEVARGAPILAQEQVQRRGHAIEARVYAEDPARGFLPQPGKIT
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCEE
YLRVPGGPGIRDDSGVYAGWVVPQWYDPMISKLVAWAPTRPQAIDRLIRALGEYVVHGIG
EEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
TNLSWLAAALDHPEFRSGDYDTGFCARNAKALIRPPDPSLERVALIAAAVAAFRRERDAA
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
EAHAARAGQGAARSGWARAGRLRALRGGGR
HHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SPTRPIEKVLVANRGEIAVRVMRTCREMRIPTVAVYSEADRGALHVRKADEAVFIGPAP
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCEEEECCCC
ARESYLSIERILDACRRTGADAVHPGYGFLSENAELARALDRAGIALVGPPAAAMDAMGV
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCHHHHHHCCC
KTTARRNMAAAGVPVVPGSEEPFAEEAEARAFAERIGFPVMIKAAAGGGGKGMKKCDRAE
HHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHH
DFAALWQSARREATAAFGDDRLYLEKFLEKPRHVEIQIFADQHGNCVWLGERECSVQRRQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCHHHHHHHH
QKVIEETPSAVLDDRLREAMGEVAVRAARAVGYVGAGTVELLVDAHRNFYFLEMNTRLQV
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCEEEEEECCEEEE
EHPVTEMVTGLDLVRMQLEVARGAPILAQEQVQRRGHAIEARVYAEDPARGFLPQPGKIT
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCEE
YLRVPGGPGIRDDSGVYAGWVVPQWYDPMISKLVAWAPTRPQAIDRLIRALGEYVVHGIG
EEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
TNLSWLAAALDHPEFRSGDYDTGFCARNAKALIRPPDPSLERVALIAAAVAAFRRERDAA
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
EAHAARAGQGAARSGWARAGRLRALRGGGR
HHHHHHCCCCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]