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The map label for this gene is ptsI [H]

Identifier: 220915292

GI number: 220915292

Start: 186859

End: 188655

Strand: Direct

Name: ptsI [H]

Synonym: A2cp1_0171

Alternate gene names: 220915292

Gene position: 186859-188655 (Clockwise)

Preceding gene: 220915291

Following gene: 220915293

Centisome position: 3.72

GC content: 73.12

Gene sequence:

>1797_bases
ATGAGCCGGTCGTCCGCCACCACGCTCGTCGGCATCGGCGCCTCGCCCGGCATCGCCATCGGGCGCTGCTGGACGATCGA
GCGGCGGCGGGTGCGGACCCCCAAGCGCCGGCTCACGCCGGAGGAGGTCGAGGCCGAGCTGGCGCGGCTGCGCACCTCGC
TCGAGATCTCCGACGTGCAGCTCGCCGAGGTGCGCGGCAAGGTGGAGGAGTCGCAGGCGCCGGGCAGCGCCGAGCACACC
GCCATCATCGACATGCACCGGATGATGCTGAAGGACGAGATGCTCGTCCTCGAGGCGCAGCGGCAGATCCGGGAGGAGCG
GCTCAACGCGGAGTGGGCGGTGAAGCGCGCCACCCGCAAGATCAAGAACGCGTTCCACGAGCAGGCCGACGACTACTTCA
AGGAGCGCCGGGCCGACGTGGACTTCGTGGGCGAGCGGATCATCAAGAACCTGCTCGGCCAGGCGCCGGACGTCGAGGAC
GTGCCGCCCGAGGGCGTGATCGTGGTGGCGCACGACCTCTCCCCCGCAGACACCGCGCTGCTCCTGCACGAGCACAAGGT
GGCCGCCTTCGTCACCGACGCCGGGGCCAAGACCTCGCACACCGCCATCGTGGCGCGCGCGTTGGAGGTGCCGGCGGTGG
TGGGGGTGGGCCGGGTGACCGCCGTCGCCGACCGCGGCGACTGGATCGTGGTGGACGGCGGGCGCGGGCTGGTGGTCATC
AACCCGAGCCCGGGCGAGCGGGCCGGCTACGAGGCCGCCCGCGAGAAGCAGCTCGCCGGCGAGCAGGCGCTGCTCGCCAC
CCGCGACCTGCCCGCCCGGACGCTCGACGACCTCACCGTCCGGCTGGCCGGCAACATCGAGTTCGCCGAGGAGGTCCCGA
GCCTGATCGCGCACGGCGGCGAGGCGGTGGGGCTCTACCGCACCGAGTTCCTGTTCATGGGCCGGTCCGACCTGCCCGGC
GAGGAGGAGCACTACCAGAACTACCGGCGCATCCTCGAGGCGCTCGCGCCGCGCCCGGTCACGATCCGCACGTTCGACCT
GGGCGGCGACAAGCTCCCGGCCGGCATGCGCGTGACCGCCGAGAACCCCGCGCTCGGCCTGCGCGCCATCCGCTACTGCC
TGCGCCAGCCGGAGATGTTCCGCGCCCAGCTCCGCGCGCTGCTGCGGGCGTCGGTGCACGGCAACCTGCGCGTCATGTTC
CCGATGATCTCCGGGGTGGCGGAGCTGCGGGCCGCGAAGCACGCGCTGCGCCAGGCGGCCGACGAGCTGCGCGCCGAGGG
CGTCGCGTTCCGGCAGGTGCCGGTGGGGATCATGGTGGAGCTGCCCAGCGCCGCCATGATCGCGGACCGGCTCGCGGCCG
AGTGCGACTTCTTCTCCATCGGGACGAACGACCTCATCCAGTACACCATCGGGATCGACCGGCAGGACAAGGACGTGGCC
TACCTGTACAAGCCGCTCCACCTGGCGGTGCTGCGCCTGCTGAAGCTCGTCTGCGACGCCGGCCGCGCCGCCGGCGTGCC
GGTGTCGATGTGCGGGGAGATGGCGGGCGAGCCGGTGAACGCGCTCGTGCTGCTCGGCCTCGGCGTCGCCGAGCTGTCCA
TGAACGGCCCGTCCATCCCGCTCGTGAAGCGGGTCGTCCGCGCCGCCCGCGCCGAGGACGGGCGGGCGCTGGTGGACCGG
CTGCTCGCCCTCACCACCGCCGACGACATCGAGCACGAGGTCCAGGCCGAGATGGCCCGCCGCTTCCCCGGCCTGCTCGA
CGGGGACGACGACGCCGAGCCGGCCGGCCAGGGCTGA

Upstream 100 bases:

>100_bases
CGGCCAAGGGCTCGCAGATCACCGTGACCTGCGACGGCGACGACGCCGACCAGGCCATGACCGCGCTCGCCAAGGTGATC
GAGAACGGGTTCGGGGAGAC

Downstream 100 bases:

>100_bases
GCCGGCGGGGCGGTCGGCGCCCGGCCGCCACGTCCGGCGGGGTCGCCCTCCGATCGGACGGACGCGGCGGCAGCGTGTCC
GCCATATTGACACCACGCGC

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MSRSSATTLVGIGASPGIAIGRCWTIERRRVRTPKRRLTPEEVEAELARLRTSLEISDVQLAEVRGKVEESQAPGSAEHT
AIIDMHRMMLKDEMLVLEAQRQIREERLNAEWAVKRATRKIKNAFHEQADDYFKERRADVDFVGERIIKNLLGQAPDVED
VPPEGVIVVAHDLSPADTALLLHEHKVAAFVTDAGAKTSHTAIVARALEVPAVVGVGRVTAVADRGDWIVVDGGRGLVVI
NPSPGERAGYEAAREKQLAGEQALLATRDLPARTLDDLTVRLAGNIEFAEEVPSLIAHGGEAVGLYRTEFLFMGRSDLPG
EEEHYQNYRRILEALAPRPVTIRTFDLGGDKLPAGMRVTAENPALGLRAIRYCLRQPEMFRAQLRALLRASVHGNLRVMF
PMISGVAELRAAKHALRQAADELRAEGVAFRQVPVGIMVELPSAAMIADRLAAECDFFSIGTNDLIQYTIGIDRQDKDVA
YLYKPLHLAVLRLLKLVCDAGRAAGVPVSMCGEMAGEPVNALVLLGLGVAELSMNGPSIPLVKRVVRAARAEDGRALVDR
LLALTTADDIEHEVQAEMARRFPGLLDGDDDAEPAGQG

Sequences:

>Translated_598_residues
MSRSSATTLVGIGASPGIAIGRCWTIERRRVRTPKRRLTPEEVEAELARLRTSLEISDVQLAEVRGKVEESQAPGSAEHT
AIIDMHRMMLKDEMLVLEAQRQIREERLNAEWAVKRATRKIKNAFHEQADDYFKERRADVDFVGERIIKNLLGQAPDVED
VPPEGVIVVAHDLSPADTALLLHEHKVAAFVTDAGAKTSHTAIVARALEVPAVVGVGRVTAVADRGDWIVVDGGRGLVVI
NPSPGERAGYEAAREKQLAGEQALLATRDLPARTLDDLTVRLAGNIEFAEEVPSLIAHGGEAVGLYRTEFLFMGRSDLPG
EEEHYQNYRRILEALAPRPVTIRTFDLGGDKLPAGMRVTAENPALGLRAIRYCLRQPEMFRAQLRALLRASVHGNLRVMF
PMISGVAELRAAKHALRQAADELRAEGVAFRQVPVGIMVELPSAAMIADRLAAECDFFSIGTNDLIQYTIGIDRQDKDVA
YLYKPLHLAVLRLLKLVCDAGRAAGVPVSMCGEMAGEPVNALVLLGLGVAELSMNGPSIPLVKRVVRAARAEDGRALVDR
LLALTTADDIEHEVQAEMARRFPGLLDGDDDAEPAGQG
>Mature_597_residues
SRSSATTLVGIGASPGIAIGRCWTIERRRVRTPKRRLTPEEVEAELARLRTSLEISDVQLAEVRGKVEESQAPGSAEHTA
IIDMHRMMLKDEMLVLEAQRQIREERLNAEWAVKRATRKIKNAFHEQADDYFKERRADVDFVGERIIKNLLGQAPDVEDV
PPEGVIVVAHDLSPADTALLLHEHKVAAFVTDAGAKTSHTAIVARALEVPAVVGVGRVTAVADRGDWIVVDGGRGLVVIN
PSPGERAGYEAAREKQLAGEQALLATRDLPARTLDDLTVRLAGNIEFAEEVPSLIAHGGEAVGLYRTEFLFMGRSDLPGE
EEHYQNYRRILEALAPRPVTIRTFDLGGDKLPAGMRVTAENPALGLRAIRYCLRQPEMFRAQLRALLRASVHGNLRVMFP
MISGVAELRAAKHALRQAADELRAEGVAFRQVPVGIMVELPSAAMIADRLAAECDFFSIGTNDLIQYTIGIDRQDKDVAY
LYKPLHLAVLRLLKLVCDAGRAAGVPVSMCGEMAGEPVNALVLLGLGVAELSMNGPSIPLVKRVVRAARAEDGRALVDRL
LALTTADDIEHEVQAEMARRFPGLLDGDDDAEPAGQG

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=566, Percent_Identity=42.0494699646643, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1789193, Length=606, Percent_Identity=33.993399339934, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI48994992, Length=501, Percent_Identity=33.9321357285429, Blast_Score=258, Evalue=8e-70,
Organism=Escherichia coli, GI1788726, Length=523, Percent_Identity=32.5047801147228, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1787994, Length=415, Percent_Identity=26.0240963855422, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI226510935, Length=202, Percent_Identity=27.2277227722772, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 65204; Mature: 65073

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSSATTLVGIGASPGIAIGRCWTIERRRVRTPKRRLTPEEVEAELARLRTSLEISDVQ
CCCCCCCEEEEECCCCCCEEEEEEEECHHHCCCCHHCCCHHHHHHHHHHHHHCCCCCCCH
LAEVRGKVEESQAPGSAEHTAIIDMHRMMLKDEMLVLEAQRQIREERLNAEWAVKRATRK
HHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IKNAFHEQADDYFKERRADVDFVGERIIKNLLGQAPDVEDVPPEGVIVVAHDLSPADTAL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHEE
LLHEHKVAAFVTDAGAKTSHTAIVARALEVPAVVGVGRVTAVADRGDWIVVDGGRGLVVI
EEECCCEEEEEECCCCCCCHHHHHHHHHHCCHHCCCCCEEEEECCCCEEEEECCCEEEEE
NPSPGERAGYEAAREKQLAGEQALLATRDLPARTLDDLTVRLAGNIEFAEEVPSLIAHGG
CCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCC
EAVGLYRTEFLFMGRSDLPGEEEHYQNYRRILEALAPRPVTIRTFDLGGDKLPAGMRVTA
CEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEC
ENPALGLRAIRYCLRQPEMFRAQLRALLRASVHGNLRVMFPMISGVAELRAAKHALRQAA
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHH
DELRAEGVAFRQVPVGIMVELPSAAMIADRLAAECDFFSIGTNDLIQYTIGIDRQDKDVA
HHHHHCCEEEEECCCEEEEECCCHHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCCCHH
YLYKPLHLAVLRLLKLVCDAGRAAGVPVSMCGEMAGEPVNALVLLGLGVAELSMNGPSIP
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCHHHCCCCCCCCH
LVKRVVRAARAEDGRALVDRLLALTTADDIEHEVQAEMARRFPGLLDGDDDAEPAGQG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRSSATTLVGIGASPGIAIGRCWTIERRRVRTPKRRLTPEEVEAELARLRTSLEISDVQ
CCCCCCEEEEECCCCCCEEEEEEEECHHHCCCCHHCCCHHHHHHHHHHHHHCCCCCCCH
LAEVRGKVEESQAPGSAEHTAIIDMHRMMLKDEMLVLEAQRQIREERLNAEWAVKRATRK
HHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IKNAFHEQADDYFKERRADVDFVGERIIKNLLGQAPDVEDVPPEGVIVVAHDLSPADTAL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHEE
LLHEHKVAAFVTDAGAKTSHTAIVARALEVPAVVGVGRVTAVADRGDWIVVDGGRGLVVI
EEECCCEEEEEECCCCCCCHHHHHHHHHHCCHHCCCCCEEEEECCCCEEEEECCCEEEEE
NPSPGERAGYEAAREKQLAGEQALLATRDLPARTLDDLTVRLAGNIEFAEEVPSLIAHGG
CCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCC
EAVGLYRTEFLFMGRSDLPGEEEHYQNYRRILEALAPRPVTIRTFDLGGDKLPAGMRVTA
CEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEC
ENPALGLRAIRYCLRQPEMFRAQLRALLRASVHGNLRVMFPMISGVAELRAAKHALRQAA
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHH
DELRAEGVAFRQVPVGIMVELPSAAMIADRLAAECDFFSIGTNDLIQYTIGIDRQDKDVA
HHHHHCCEEEEECCCEEEEECCCHHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCCCHH
YLYKPLHLAVLRLLKLVCDAGRAAGVPVSMCGEMAGEPVNALVLLGLGVAELSMNGPSIP
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCHHHCCCCCCCCH
LVKRVVRAARAEDGRALVDRLLALTTADDIEHEVQAEMARRFPGLLDGDDDAEPAGQG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]