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The map label for this gene is metK

Identifier: 220915293

GI number: 220915293

Start: 188813

End: 189985

Strand: Direct

Name: metK

Synonym: A2cp1_0172

Alternate gene names: 220915293

Gene position: 188813-189985 (Clockwise)

Preceding gene: 220915292

Following gene: 220915294

Centisome position: 3.75

GC content: 70.76

Gene sequence:

>1173_bases
ATGCCGCTCCAGGACTTCCTCTTCACCTCCGAGTCGGTCACGGAGGGCCATCCGGACAAGATGGCGGACCAGATCTCCGA
CGCCGTGCTCGACGCGGTGCTCCGCCAGGACCCGAAGGGCCGGGTGGCCTGCGAGACGCTGCTCAAGACCGGCTACGTGA
TGATCGCCGGCGAGATCACCACCAAGGCGCGCATCGACTACCCGAAGCTCGCCCGCGAGACGGTGCGCCGCATCGGCTAC
ACCAGCGGCGACATGGGCTTCGACGCCAACACCTGCGCGGTGCTGGTGGCGGTGGACCAGCAGTCGCCGGACATCGGCCA
GGGCGTGGACACCGGCGGCGCCGGCGACCAGGGCATGATGTTCGGCTACGCCTGCGACGAGACGCCGGAGCTGATGCCGG
CGCCCATCCAGTACGCGCACGCGGTGACGAAGCAGCTCGCGAAGGCGCGCCGCGCCGGCCTCGACTTCCTCCGCCCCGAC
GGCAAGAGCCAGGTGTCGGTCGAGTACCGCGACGGCCGGCCGGTCCGCATCGACACGGTGGTGGTCTCCACCCAGCACGC
CGAGTCGGTCTCGAACAAGCGGCTGCACGAGGCGGTGCGCGAGCAGGTGATCGCGAAGGCGCTCCCGAAGCGGCTCCTCG
ATCGCAAGACGCGGATCCTCATCAACCCCACCGGCCGGTTCGTGATCGGCGGCCCCATGGGCGACACCGGCGTCACCGGG
CGGAAGATCATCGTCGACACCTACGGCGGCATGGGCCGCCACGGCGGCGGCGCGTTCAGCGGCAAGGACCCGTCGAAGGT
GGACCGCTCCGCCGCGTACATGGGGCGCTACATCGCGAAGAACGTGGTCGCGGCCGGCCTGGCCTCGCGCTGCGAGGTGC
AGGTGGCGTACGCCATCGGCGTGGCCGAGCCGGTCTCGGTGATGGTGGACACGTTCGGCACCGCCAAGGTGCCGGAGGGG
AAGATCGCCCGCGCCGTCCGCGAGGTGTTCGGCCTCACCCCGCGCGCCATCATCGAGGGGCTCGACCTGCTGCGGCCGGT
GTACGAGAAGACCGCCGCGTACGGCCACTTCGGCCGGACCGAGAAGACGTTCACCTGGGAGCGCACCGACAAGAAGGACG
CGCTCGCCGACGCCGCCGGCCTCTCGAAGATCCGGGCGGTCGCGTCCGTCTGA

Upstream 100 bases:

>100_bases
ACGGACGCGGCGGCAGCGTGTCCGCCATATTGACACCACGCGCGCCGGGCGCGTACCTTGCCCGTCCCGCCCGGGCACCC
CCGCCTCCGGAGGAGAACAC

Downstream 100 bases:

>100_bases
CGGGGCTGCGCCCCGCCCCCCGGAGCGGAGCTCCGCGGGGCCCACCTTGCTGCGCGCGTCCCGTGACCTCGCGCGCCCGC
CTTCGCGGGCTCGCACGAGG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MPLQDFLFTSESVTEGHPDKMADQISDAVLDAVLRQDPKGRVACETLLKTGYVMIAGEITTKARIDYPKLARETVRRIGY
TSGDMGFDANTCAVLVAVDQQSPDIGQGVDTGGAGDQGMMFGYACDETPELMPAPIQYAHAVTKQLAKARRAGLDFLRPD
GKSQVSVEYRDGRPVRIDTVVVSTQHAESVSNKRLHEAVREQVIAKALPKRLLDRKTRILINPTGRFVIGGPMGDTGVTG
RKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVAAGLASRCEVQVAYAIGVAEPVSVMVDTFGTAKVPEG
KIARAVREVFGLTPRAIIEGLDLLRPVYEKTAAYGHFGRTEKTFTWERTDKKDALADAAGLSKIRAVASV

Sequences:

>Translated_390_residues
MPLQDFLFTSESVTEGHPDKMADQISDAVLDAVLRQDPKGRVACETLLKTGYVMIAGEITTKARIDYPKLARETVRRIGY
TSGDMGFDANTCAVLVAVDQQSPDIGQGVDTGGAGDQGMMFGYACDETPELMPAPIQYAHAVTKQLAKARRAGLDFLRPD
GKSQVSVEYRDGRPVRIDTVVVSTQHAESVSNKRLHEAVREQVIAKALPKRLLDRKTRILINPTGRFVIGGPMGDTGVTG
RKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVAAGLASRCEVQVAYAIGVAEPVSVMVDTFGTAKVPEG
KIARAVREVFGLTPRAIIEGLDLLRPVYEKTAAYGHFGRTEKTFTWERTDKKDALADAAGLSKIRAVASV
>Mature_389_residues
PLQDFLFTSESVTEGHPDKMADQISDAVLDAVLRQDPKGRVACETLLKTGYVMIAGEITTKARIDYPKLARETVRRIGYT
SGDMGFDANTCAVLVAVDQQSPDIGQGVDTGGAGDQGMMFGYACDETPELMPAPIQYAHAVTKQLAKARRAGLDFLRPDG
KSQVSVEYRDGRPVRIDTVVVSTQHAESVSNKRLHEAVREQVIAKALPKRLLDRKTRILINPTGRFVIGGPMGDTGVTGR
KIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVAAGLASRCEVQVAYAIGVAEPVSVMVDTFGTAKVPEGK
IARAVREVFGLTPRAIIEGLDLLRPVYEKTAAYGHFGRTEKTFTWERTDKKDALADAAGLSKIRAVASV

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=373, Percent_Identity=56.0321715817694, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI5174529, Length=373, Percent_Identity=55.4959785522788, Blast_Score=397, Evalue=1e-110,
Organism=Escherichia coli, GI1789311, Length=381, Percent_Identity=65.6167979002625, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17551082, Length=374, Percent_Identity=57.7540106951872, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17538494, Length=379, Percent_Identity=55.9366754617414, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17538498, Length=379, Percent_Identity=56.2005277044855, Blast_Score=409, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25145633, Length=402, Percent_Identity=53.4825870646766, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32565905, Length=379, Percent_Identity=50.6596306068602, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI32565911, Length=327, Percent_Identity=53.2110091743119, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI25145635, Length=350, Percent_Identity=50.5714285714286, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=53.4883720930233, Blast_Score=298, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=60.9195402298851, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI32565907, Length=50, Percent_Identity=68, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=377, Percent_Identity=56.2334217506631, Blast_Score=416, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6323209, Length=374, Percent_Identity=56.4171122994652, Blast_Score=411, Evalue=1e-116,
Organism=Drosophila melanogaster, GI45552159, Length=374, Percent_Identity=54.0106951871658, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580529, Length=374, Percent_Identity=54.0106951871658, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580537, Length=374, Percent_Identity=54.0106951871658, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580533, Length=374, Percent_Identity=54.0106951871658, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580535, Length=374, Percent_Identity=54.0106951871658, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580531, Length=374, Percent_Identity=54.0106951871658, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=53.475935828877, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=53.475935828877, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580541, Length=361, Percent_Identity=52.0775623268698, Blast_Score=357, Evalue=8e-99,
Organism=Drosophila melanogaster, GI24580539, Length=118, Percent_Identity=60.1694915254237, Blast_Score=148, Evalue=5e-36,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_ANAD2 (B8J8T3)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002490597.1
- ProteinModelPortal:   B8J8T3
- SMR:   B8J8T3
- GeneID:   7298407
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_0172
- HOGENOM:   HBG443662
- ProtClustDB:   PRK05250
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42006; Mature: 41874

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLQDFLFTSESVTEGHPDKMADQISDAVLDAVLRQDPKGRVACETLLKTGYVMIAGEIT
CCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEECCC
TKARIDYPKLARETVRRIGYTSGDMGFDANTCAVLVAVDQQSPDIGQGVDTGGAGDQGMM
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCE
FGYACDETPELMPAPIQYAHAVTKQLAKARRAGLDFLRPDGKSQVSVEYRDGRPVRIDTV
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEE
VVSTQHAESVSNKRLHEAVREQVIAKALPKRLLDRKTRILINPTGRFVIGGPMGDTGVTG
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCC
RKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVAAGLASRCEVQVAYAIG
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEC
VAEPVSVMVDTFGTAKVPEGKIARAVREVFGLTPRAIIEGLDLLRPVYEKTAAYGHFGRT
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EKTFTWERTDKKDALADAAGLSKIRAVASV
CCEEEECCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLQDFLFTSESVTEGHPDKMADQISDAVLDAVLRQDPKGRVACETLLKTGYVMIAGEIT
CCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEECCC
TKARIDYPKLARETVRRIGYTSGDMGFDANTCAVLVAVDQQSPDIGQGVDTGGAGDQGMM
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCE
FGYACDETPELMPAPIQYAHAVTKQLAKARRAGLDFLRPDGKSQVSVEYRDGRPVRIDTV
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEE
VVSTQHAESVSNKRLHEAVREQVIAKALPKRLLDRKTRILINPTGRFVIGGPMGDTGVTG
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCC
RKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVAAGLASRCEVQVAYAIG
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEC
VAEPVSVMVDTFGTAKVPEGKIARAVREVFGLTPRAIIEGLDLLRPVYEKTAAYGHFGRT
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EKTFTWERTDKKDALADAAGLSKIRAVASV
CCEEEECCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA