Click here to switch to the map view.

The map label for this gene is mrcA [H]

Identifier: 220915195

GI number: 220915195

Start: 82611

End: 85169

Strand: Direct

Name: mrcA [H]

Synonym: A2cp1_0074

Alternate gene names: 220915195

Gene position: 82611-85169 (Clockwise)

Preceding gene: 220915194

Following gene: 220915196

Centisome position: 1.64

GC content: 72.18

Gene sequence:

>2559_bases
ATGCGCGAGCCCGGCGATCCACAGCAGCCACCCGAGGAGCCCTCCTCGCGCGTGGTCCTCGACGGGCTGCCGCCCGAGCG
CCCGCTGCGCCGGCGGCTCCTCCGCTGGGGGCTGTTCGCGGCGATCGCGGCGGTGAACGCCGTGATCGTCGCGGCGGTGG
TGGGGTGGTTCGTCTTCTCGCGCGGGCTGCCGGACATCCCCACGCTGGAGCAGTACCGCCCGCCCATCATCACCGAGATG
ATCTCGGCCGACGGCCAGATCGCCGGCGAGTTCTTCGTGGAGCGCCGCAAGGTCGTCCCCTACGCGCGCATCCCGAGGCG
CGTCATCCAGGCGTTCATCGCCTCGGAGGACAAGAACTTCTTCGAGCACGGCGGCATCGACCTGCTCGGCACGCTCCGCG
CCGCGGTGAAGACGTACGTGCTCCGCTCGCGGATCCAGGGCGCCTCGACGCTCACGCAGCAGACCGCCAAGGCCATCCTC
ATCAGCGCCGAGGGCTTCGACCGCGGCACCGAGCGCAGCCTGCGCCGCAAGATCCGGGAGCTGATCCTGGCGCGGCGCCT
CGAGGCGCGCTTCACGAAGGAGCAGATCCTCTGGATGTACCTGAACGGCGTGTACCTCGGGCACCACAGCTACGGGGTGC
AGTCGGCCGCGGAGAACTACTACCGCAAGAACGTGGAGGACCTGACGCTGGAGGAGGCGGCCCTCATCGCCGGGCTGCCG
CAGGCGCCCTCGCGCTACTCGCCGTTCTCGCGGCCGGAGGCGGCCGCCGAGCGGCGGCGCTACGTGCTCCGGCGCATGGC
CGAGGAGGGGATGATCACCGAGGGCGAGCGCCGCTCCGCCGAGAAGGCCGAGGTGAAGGTGTTCGGCGTGGACGACGTGT
TCCGCGAGACCGCCCCGTTCTACGTGGAGACCGTGCGCCGGCAGGTGGTGGACCGCTACGGCAACGACCGGCTGCTGCAC
GACGGCCTGCGGGTCGAGATGGCGATGGACCTCGAGAAGCAGCGCGCCGCGCAGGCGGCGGTGCTGAAGGGGCTCGTCGA
GGTGGACCAGCGGCAGGGCTTCACCGGGCCGGTGGCGAAGGTGGCCGGCGCGGAGCGGACCGCGCTCGCCGCGCGCCTCG
CGAAGGCGTGGCCGAAGGGCACGCTCCGCCCCGGCGACTTCTGCGTCGGCATCGTGGATCGGGTGGACGACAAGGCCGGC
GTCGTCGAGGTGGAGGTGGGCGAGACGCGCGGCGTGCTGCCCATCTCCGGCATGCGCTGGGCCCGCAGGCCGAACCCGGT
GGTGTCCTACGCGGACTCGCTCCTCTCCCGCCCCTCGAGCGCGCTCCACGCCGGCGACGTGGTGGTGCTGAAGCGCGTCG
AGCGCGCCGAGCTGCAGCGGAAGGAGGCGCAGCTCGGGGTGGGCCGGCCGAAGGACGTGCCCGAGGCGCCGGTGCTGTTC
TCGCTGCAGCAGGATCCCACGCTGCAGGGCGCGCTCGTGGCGGTCGATCCCGGGACGGCCTACGTCACCGCCATGGTGGG
CGGCTACGACTTCGAGGCGTCCGAGTTCAACCGCGCCTTCCAGGCCTGCCGCCAGCCGGGCTCGGCGTTCAAGCCGGTCG
TCTACTCCGCCGCGGTGGAGAAGCTCGACTACACGCCGGCCACCATCCTCACCGACGCGCCCATCGTGTTCCGCGACGAC
GAGTCGAGCTGGAAGCCGCAGAACTACGGCGAGGACTTCAAGGGCGACGTCACGCTGCGCACCGCGCTCGTCAACTCGAT
GAACATCCCGGCGGTGAAGACCGCCGAGGCGCTCAACGCGAAGCTCGGGCCGGCGGGGCTGGGCGACTGGGCGAAGACGC
TCGGGATCACCACGCCGGTGAAGCAGGAGCTGGGCAGCGCGCTCGGCTCGTCCTGCGTCACGCCCTGGGAGCTCACCAAC
GTGTACGCGCTGTTCGCGCGGTACGGCGAGAAGCGGCCCAGCGCGTTCGTGAAGCGCGTGCTGGATCGCGACGGGCGCGT
CCTCGAGGACCACGCCGACTACCGCGACCCCTGGGTCGGGCTCGACGAGCGGCTGGCCGGCGCGGTGGCGGAGGTGACGC
GCCCGCGCGAGCAGGTGATGGACCCGCGCAGCGCCTACATCCTGGTCCGCCTCATGCGCGAGGTCGCGACCGTCGGGACC
GGCGCGCGCGCGGCCGCGCTGGGGAAGCCGGCGGCCGGCAAGACCGGCACCACCAACGACTCCTTCGACACCTGGTTCAT
GGGCTTCACCCGCGACCTCGCCACCGGCGTCTGGCTCGGCTACGACGTCAACGTCACCCCGCTCGGCCGCTACGAGACCG
GCGGCCACGCCGCGCTGCCCATCTGGCTCGACTTCATGTCGGCGGCGCTGAAGGGCAGGGCGCAGCCCGAGTTCGAGGCG
CCGTCGGGCATCGTCGAGGTGCGGATCGACCCGGCCACCGGCAAGGCGGTGGCGGACGGCGCCGGCGGCGTGCCGGAGCC
GTTCAAGCAGGGGACCGAGCCCGTGCTCGCCGATGCCGAGAAGAAGCAGGTCGAGGTGCAGGACCTGTTCATGCAGTAG

Upstream 100 bases:

>100_bases
CCGGGTCGCCCACCGGCGCACACGCGCCGGCCGCGCGCCGCGACGGACCGCACCGCGAGCGTTGAACTCCGGGGCGGGGC
ACGCATAGATTCGGCCACCC

Downstream 100 bases:

>100_bases
TCCGCAGGAGATCGGCCCGGCTGCGTCCCGCGGTCGTGGTTCGCGCCGGCCTCCCGGGTGCGCACGCGGCGTGCGCGGAG
GTGCGTGCGCCGCCGCCGCG

Product: penicillin-binding protein, 1A family

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 852; Mature: 852

Protein sequence:

>852_residues
MREPGDPQQPPEEPSSRVVLDGLPPERPLRRRLLRWGLFAAIAAVNAVIVAAVVGWFVFSRGLPDIPTLEQYRPPIITEM
ISADGQIAGEFFVERRKVVPYARIPRRVIQAFIASEDKNFFEHGGIDLLGTLRAAVKTYVLRSRIQGASTLTQQTAKAIL
ISAEGFDRGTERSLRRKIRELILARRLEARFTKEQILWMYLNGVYLGHHSYGVQSAAENYYRKNVEDLTLEEAALIAGLP
QAPSRYSPFSRPEAAAERRRYVLRRMAEEGMITEGERRSAEKAEVKVFGVDDVFRETAPFYVETVRRQVVDRYGNDRLLH
DGLRVEMAMDLEKQRAAQAAVLKGLVEVDQRQGFTGPVAKVAGAERTALAARLAKAWPKGTLRPGDFCVGIVDRVDDKAG
VVEVEVGETRGVLPISGMRWARRPNPVVSYADSLLSRPSSALHAGDVVVLKRVERAELQRKEAQLGVGRPKDVPEAPVLF
SLQQDPTLQGALVAVDPGTAYVTAMVGGYDFEASEFNRAFQACRQPGSAFKPVVYSAAVEKLDYTPATILTDAPIVFRDD
ESSWKPQNYGEDFKGDVTLRTALVNSMNIPAVKTAEALNAKLGPAGLGDWAKTLGITTPVKQELGSALGSSCVTPWELTN
VYALFARYGEKRPSAFVKRVLDRDGRVLEDHADYRDPWVGLDERLAGAVAEVTRPREQVMDPRSAYILVRLMREVATVGT
GARAAALGKPAAGKTGTTNDSFDTWFMGFTRDLATGVWLGYDVNVTPLGRYETGGHAALPIWLDFMSAALKGRAQPEFEA
PSGIVEVRIDPATGKAVADGAGGVPEPFKQGTEPVLADAEKKQVEVQDLFMQ

Sequences:

>Translated_852_residues
MREPGDPQQPPEEPSSRVVLDGLPPERPLRRRLLRWGLFAAIAAVNAVIVAAVVGWFVFSRGLPDIPTLEQYRPPIITEM
ISADGQIAGEFFVERRKVVPYARIPRRVIQAFIASEDKNFFEHGGIDLLGTLRAAVKTYVLRSRIQGASTLTQQTAKAIL
ISAEGFDRGTERSLRRKIRELILARRLEARFTKEQILWMYLNGVYLGHHSYGVQSAAENYYRKNVEDLTLEEAALIAGLP
QAPSRYSPFSRPEAAAERRRYVLRRMAEEGMITEGERRSAEKAEVKVFGVDDVFRETAPFYVETVRRQVVDRYGNDRLLH
DGLRVEMAMDLEKQRAAQAAVLKGLVEVDQRQGFTGPVAKVAGAERTALAARLAKAWPKGTLRPGDFCVGIVDRVDDKAG
VVEVEVGETRGVLPISGMRWARRPNPVVSYADSLLSRPSSALHAGDVVVLKRVERAELQRKEAQLGVGRPKDVPEAPVLF
SLQQDPTLQGALVAVDPGTAYVTAMVGGYDFEASEFNRAFQACRQPGSAFKPVVYSAAVEKLDYTPATILTDAPIVFRDD
ESSWKPQNYGEDFKGDVTLRTALVNSMNIPAVKTAEALNAKLGPAGLGDWAKTLGITTPVKQELGSALGSSCVTPWELTN
VYALFARYGEKRPSAFVKRVLDRDGRVLEDHADYRDPWVGLDERLAGAVAEVTRPREQVMDPRSAYILVRLMREVATVGT
GARAAALGKPAAGKTGTTNDSFDTWFMGFTRDLATGVWLGYDVNVTPLGRYETGGHAALPIWLDFMSAALKGRAQPEFEA
PSGIVEVRIDPATGKAVADGAGGVPEPFKQGTEPVLADAEKKQVEVQDLFMQ
>Mature_852_residues
MREPGDPQQPPEEPSSRVVLDGLPPERPLRRRLLRWGLFAAIAAVNAVIVAAVVGWFVFSRGLPDIPTLEQYRPPIITEM
ISADGQIAGEFFVERRKVVPYARIPRRVIQAFIASEDKNFFEHGGIDLLGTLRAAVKTYVLRSRIQGASTLTQQTAKAIL
ISAEGFDRGTERSLRRKIRELILARRLEARFTKEQILWMYLNGVYLGHHSYGVQSAAENYYRKNVEDLTLEEAALIAGLP
QAPSRYSPFSRPEAAAERRRYVLRRMAEEGMITEGERRSAEKAEVKVFGVDDVFRETAPFYVETVRRQVVDRYGNDRLLH
DGLRVEMAMDLEKQRAAQAAVLKGLVEVDQRQGFTGPVAKVAGAERTALAARLAKAWPKGTLRPGDFCVGIVDRVDDKAG
VVEVEVGETRGVLPISGMRWARRPNPVVSYADSLLSRPSSALHAGDVVVLKRVERAELQRKEAQLGVGRPKDVPEAPVLF
SLQQDPTLQGALVAVDPGTAYVTAMVGGYDFEASEFNRAFQACRQPGSAFKPVVYSAAVEKLDYTPATILTDAPIVFRDD
ESSWKPQNYGEDFKGDVTLRTALVNSMNIPAVKTAEALNAKLGPAGLGDWAKTLGITTPVKQELGSALGSSCVTPWELTN
VYALFARYGEKRPSAFVKRVLDRDGRVLEDHADYRDPWVGLDERLAGAVAEVTRPREQVMDPRSAYILVRLMREVATVGT
GARAAALGKPAAGKTGTTNDSFDTWFMGFTRDLATGVWLGYDVNVTPLGRYETGGHAALPIWLDFMSAALKGRAQPEFEA
PSGIVEVRIDPATGKAVADGAGGVPEPFKQGTEPVLADAEKKQVEVQDLFMQ

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=863, Percent_Identity=31.0544611819235, Blast_Score=339, Evalue=3e-94,
Organism=Escherichia coli, GI1786343, Length=190, Percent_Identity=35.7894736842105, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788867, Length=212, Percent_Identity=31.6037735849057, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1789601, Length=160, Percent_Identity=35.625, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 93644; Mature: 93644

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREPGDPQQPPEEPSSRVVLDGLPPERPLRRRLLRWGLFAAIAAVNAVIVAAVVGWFVFS
CCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGLPDIPTLEQYRPPIITEMISADGQIAGEFFVERRKVVPYARIPRRVIQAFIASEDKNF
CCCCCCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCH
FEHGGIDLLGTLRAAVKTYVLRSRIQGASTLTQQTAKAILISAEGFDRGTERSLRRKIRE
HHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHH
LILARRLEARFTKEQILWMYLNGVYLGHHSYGVQSAAENYYRKNVEDLTLEEAALIAGLP
HHHHHHHHHHHHHHHEEEEEECCEEEECCHHCHHHHHHHHHHCCCHHHHHHHHHHHHCCC
QAPSRYSPFSRPEAAAERRRYVLRRMAEEGMITEGERRSAEKAEVKVFGVDDVFRETAPF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECHHHHHHCCHH
YVETVRRQVVDRYGNDRLLHDGLRVEMAMDLEKQRAAQAAVLKGLVEVDQRQGFTGPVAK
HHHHHHHHHHHHCCCCCEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
VAGAERTALAARLAKAWPKGTLRPGDFCVGIVDRVDDKAGVVEVEVGETRGVLPISGMRW
HCCCHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
ARRPNPVVSYADSLLSRPSSALHAGDVVVLKRVERAELQRKEAQLGVGRPKDVPEAPVLF
CCCCCHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
SLQQDPTLQGALVAVDPGTAYVTAMVGGYDFEASEFNRAFQACRQPGSAFKPVVYSAAVE
EECCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
KLDYTPATILTDAPIVFRDDESSWKPQNYGEDFKGDVTLRTALVNSMNIPAVKTAEALNA
HHCCCCHHHEECCCEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCHHHHHHCC
KLGPAGLGDWAKTLGITTPVKQELGSALGSSCVTPWELTNVYALFARYGEKRPSAFVKRV
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
LDRDGRVLEDHADYRDPWVGLDERLAGAVAEVTRPREQVMDPRSAYILVRLMREVATVGT
HCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHCC
GARAAALGKPAAGKTGTTNDSFDTWFMGFTRDLATGVWLGYDVNVTPLGRYETGGHAALP
CCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCEEHH
IWLDFMSAALKGRAQPEFEAPSGIVEVRIDPATGKAVADGAGGVPEPFKQGTEPVLADAE
HHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCEECCCCCCCCCHHHCCCCCHHCCCH
KKQVEVQDLFMQ
HHHHHHHHHHCC
>Mature Secondary Structure
MREPGDPQQPPEEPSSRVVLDGLPPERPLRRRLLRWGLFAAIAAVNAVIVAAVVGWFVFS
CCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGLPDIPTLEQYRPPIITEMISADGQIAGEFFVERRKVVPYARIPRRVIQAFIASEDKNF
CCCCCCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCH
FEHGGIDLLGTLRAAVKTYVLRSRIQGASTLTQQTAKAILISAEGFDRGTERSLRRKIRE
HHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHH
LILARRLEARFTKEQILWMYLNGVYLGHHSYGVQSAAENYYRKNVEDLTLEEAALIAGLP
HHHHHHHHHHHHHHHEEEEEECCEEEECCHHCHHHHHHHHHHCCCHHHHHHHHHHHHCCC
QAPSRYSPFSRPEAAAERRRYVLRRMAEEGMITEGERRSAEKAEVKVFGVDDVFRETAPF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECHHHHHHCCHH
YVETVRRQVVDRYGNDRLLHDGLRVEMAMDLEKQRAAQAAVLKGLVEVDQRQGFTGPVAK
HHHHHHHHHHHHCCCCCEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
VAGAERTALAARLAKAWPKGTLRPGDFCVGIVDRVDDKAGVVEVEVGETRGVLPISGMRW
HCCCHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
ARRPNPVVSYADSLLSRPSSALHAGDVVVLKRVERAELQRKEAQLGVGRPKDVPEAPVLF
CCCCCHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
SLQQDPTLQGALVAVDPGTAYVTAMVGGYDFEASEFNRAFQACRQPGSAFKPVVYSAAVE
EECCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
KLDYTPATILTDAPIVFRDDESSWKPQNYGEDFKGDVTLRTALVNSMNIPAVKTAEALNA
HHCCCCHHHEECCCEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCHHHHHHCC
KLGPAGLGDWAKTLGITTPVKQELGSALGSSCVTPWELTNVYALFARYGEKRPSAFVKRV
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
LDRDGRVLEDHADYRDPWVGLDERLAGAVAEVTRPREQVMDPRSAYILVRLMREVATVGT
HCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHCC
GARAAALGKPAAGKTGTTNDSFDTWFMGFTRDLATGVWLGYDVNVTPLGRYETGGHAALP
CCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCEEHH
IWLDFMSAALKGRAQPEFEAPSGIVEVRIDPATGKAVADGAGGVPEPFKQGTEPVLADAE
HHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCEECCCCCCCCCHHHCCCCCHHCCCH
KKQVEVQDLFMQ
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043; 7901733 [H]