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The map label for this gene is pilJ [H]

Identifier: 220915196

GI number: 220915196

Start: 85371

End: 87437

Strand: Direct

Name: pilJ [H]

Synonym: A2cp1_0075

Alternate gene names: 220915196

Gene position: 85371-87437 (Clockwise)

Preceding gene: 220915195

Following gene: 220915198

Centisome position: 1.7

GC content: 70.92

Gene sequence:

>2067_bases
ATGCGTCCAAAGCTCGCCCACCTCGGCGTGAAGCGGATCCTGTTCCTGTCCATCGCCGGCGCGCTGGCCATCGCCGGCGT
GCTCACGTCGATCGCGGTGAACCGCGAGATCCACGTCGCGCTGGAGCGCGCCGCCGTGCACCGCGGCGAGGCCGACGCCC
GCGTCTACGCCTCCGCGCTCGCCCCGGCGCTCGCGGCCGGCGATCCGGCGCGCATCGCGGCGCGGGTGGACGAGGACTGG
GACGAGGAGTCGTTCGCGTACGTGGTGGTGGTCCGCGAGGACTGGTCCGTCGCCGCGAGGCACCTCTCCCGCAACCTGGG
CCGCAGCGCCGAGGAGGTGGTGGCCTGGCACCAGGCGGCGGGCGGCGCGGCGCAGCCGCAGGCGCCGGGCGTGCTCGCCT
TCACGCGCCCGGTCGAGGGCGCGGGGGGCGGCGCGCGCGCCGGGTACGTCCTGCTCGGCCTCGACCGCGACCCGCTCGAC
GGCCAGGCCGGGCGCGCGGGCTGGTACATCTTCGCGCTCCTGGTGGGCGGCGGGATGGTGCTGGCGGGGCTCGTGTACGT
GTTCGTCTCGCGCCTCGTGCTCCGGCCGCTCGACGAGATGAGCGCGGTGGCGCGCCGGGTCTCCGACTGCGACCTGACCG
CCCGCTCCGCCCAGCTCGGCCCGGACGAGCTGGGGACGCTGGCCGAGTCGCTGAACCGCATCGGCGAGAACCTGGCCGCG
ACGCTGTCCCGCGTGCAGAACGTGACCGGCGGGGTGGCGACCGTCATCGACCGGATCTCGCGCACCGGCTCGGCGGTCTC
CTCCGGCGCCGGCACCGTCACGGCGCGGGTGGTGGACACCTCCTCTTCGATGAGCCAGATGATCTCCTCGCTGAAGGGGA
TCGCCGACAACGTCGAGGTGCTGGCGCAGAGCGCCGAGGAGTCCTCCTCGTCGATCCTGGAGATGGCCGCCACCAACGAC
GAGGTGGCCGAGAACATCCAGGCGCTCGCGGCCAGCGTCGAGGAGACCACCGCCGCGATCGAGCAGATGACCTACTCGAT
CAAGGAGGTGGCGAAGAACATCGAGGACCTGTCCGCCACCACCGAGGAGACGTCGTCCTCGATGAACGAGATGGACGTCT
CCATCTCGCAGGTGGAGTCGAACGCGAACGAGACGGCGCGCCTCTCCGAGCAGGCGCAGCGCGACGCGGAGTCGGGGGCG
GAGGCGCTCTCGCGCACGCTCCTCGGCATCGACAAGATCAAGGAGTCCTCCAAGGAGGCGGCCAGCGTCATCGAGGCGCT
CGGCGCCAAGATCGCCGCCATCGGCAACATCCTCAACGTGATCGACGACGTGGCCGAGCAGACCAACCTGCTCGCGCTGA
ACGCCGCCATCCTGGCGGCGCAGTCGGGCGAGCACGGCAAGGGCTTCGCGGTGGTGGCGGACGAGATCAAGGACCTGGCC
GAGCGCACCGGCGCGTCCACCAAGGAGATCTCCGAGCTCATCCGCGCGGTGCAGGACCAGTCGCGCAACGCCATCCAGGC
GATGGACCGCGGCGTCCGCAACGTCGAGGAGGGCGTGCGGCTCGGGCAGGAGACCGAGACCGCGCTGAAGAAGATCCAGG
AGTCGAGCCAGAAGTCCACGCAGATGGTGAAGGCCATCGCGCGGGCCACCCTGGAGCAGGCGCGCGGGTCGAAGCAGGTG
ACCATGGCGATCAACCGCATCGCCGAGACGGTGCAGCAGATCGCGAGCGCGACCGCCGAGCAGGCGCGCGGCTCCGAGCA
GATCATGAAGAGCGCCGAGCGGATGAAGGCGATCACGAAGCACGTGGAGCGCTCGGCGCAGGAGCAGGCGCGCGGCTCGA
AGCAGATCACCCGCGCCATCGAGTCGATCTCGGAGATGGTCCACCACCTGAACCGCGCCCAGAAGGAGCAGACCAAGGGC
TCCGAGCAGGTCATGACGGCGGTGGTGCAGATCAAGCAGGTGGCCGAGGCGCAGACGCACTCGGTCCGTGACCTCGAGGC
CGCCATCGTCGATCTCGCCTCGCAGGCGGAGGTGCTCCGCGGCGAGGTGAGGAGATTCAGGATCTAG

Upstream 100 bases:

>100_bases
GCGCCGGCCGTTCCCTTGCCTCGATCCCGACCCGCCGGTAGCATCGCGCCGAACCGCCGGAAAATCGCGTGCTTCAGCCC
GCGCCGGCGAGAAGGGCCCG

Downstream 100 bases:

>100_bases
GGGAACAGCGGGCCGACCGGCGCGCCCGCGAGGTCACGGGCCCCGCAGCAGGAGTGGGGCCCCGGCGGCTCCGCCGGCGG
GGCGAGGGCGCGGCCCTCGG

Product: methyl-accepting chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 688; Mature: 688

Protein sequence:

>688_residues
MRPKLAHLGVKRILFLSIAGALAIAGVLTSIAVNREIHVALERAAVHRGEADARVYASALAPALAAGDPARIAARVDEDW
DEESFAYVVVVREDWSVAARHLSRNLGRSAEEVVAWHQAAGGAAQPQAPGVLAFTRPVEGAGGGARAGYVLLGLDRDPLD
GQAGRAGWYIFALLVGGGMVLAGLVYVFVSRLVLRPLDEMSAVARRVSDCDLTARSAQLGPDELGTLAESLNRIGENLAA
TLSRVQNVTGGVATVIDRISRTGSAVSSGAGTVTARVVDTSSSMSQMISSLKGIADNVEVLAQSAEESSSSILEMAATND
EVAENIQALAASVEETTAAIEQMTYSIKEVAKNIEDLSATTEETSSSMNEMDVSISQVESNANETARLSEQAQRDAESGA
EALSRTLLGIDKIKESSKEAASVIEALGAKIAAIGNILNVIDDVAEQTNLLALNAAILAAQSGEHGKGFAVVADEIKDLA
ERTGASTKEISELIRAVQDQSRNAIQAMDRGVRNVEEGVRLGQETETALKKIQESSQKSTQMVKAIARATLEQARGSKQV
TMAINRIAETVQQIASATAEQARGSEQIMKSAERMKAITKHVERSAQEQARGSKQITRAIESISEMVHHLNRAQKEQTKG
SEQVMTAVVQIKQVAEAQTHSVRDLEAAIVDLASQAEVLRGEVRRFRI

Sequences:

>Translated_688_residues
MRPKLAHLGVKRILFLSIAGALAIAGVLTSIAVNREIHVALERAAVHRGEADARVYASALAPALAAGDPARIAARVDEDW
DEESFAYVVVVREDWSVAARHLSRNLGRSAEEVVAWHQAAGGAAQPQAPGVLAFTRPVEGAGGGARAGYVLLGLDRDPLD
GQAGRAGWYIFALLVGGGMVLAGLVYVFVSRLVLRPLDEMSAVARRVSDCDLTARSAQLGPDELGTLAESLNRIGENLAA
TLSRVQNVTGGVATVIDRISRTGSAVSSGAGTVTARVVDTSSSMSQMISSLKGIADNVEVLAQSAEESSSSILEMAATND
EVAENIQALAASVEETTAAIEQMTYSIKEVAKNIEDLSATTEETSSSMNEMDVSISQVESNANETARLSEQAQRDAESGA
EALSRTLLGIDKIKESSKEAASVIEALGAKIAAIGNILNVIDDVAEQTNLLALNAAILAAQSGEHGKGFAVVADEIKDLA
ERTGASTKEISELIRAVQDQSRNAIQAMDRGVRNVEEGVRLGQETETALKKIQESSQKSTQMVKAIARATLEQARGSKQV
TMAINRIAETVQQIASATAEQARGSEQIMKSAERMKAITKHVERSAQEQARGSKQITRAIESISEMVHHLNRAQKEQTKG
SEQVMTAVVQIKQVAEAQTHSVRDLEAAIVDLASQAEVLRGEVRRFRI
>Mature_688_residues
MRPKLAHLGVKRILFLSIAGALAIAGVLTSIAVNREIHVALERAAVHRGEADARVYASALAPALAAGDPARIAARVDEDW
DEESFAYVVVVREDWSVAARHLSRNLGRSAEEVVAWHQAAGGAAQPQAPGVLAFTRPVEGAGGGARAGYVLLGLDRDPLD
GQAGRAGWYIFALLVGGGMVLAGLVYVFVSRLVLRPLDEMSAVARRVSDCDLTARSAQLGPDELGTLAESLNRIGENLAA
TLSRVQNVTGGVATVIDRISRTGSAVSSGAGTVTARVVDTSSSMSQMISSLKGIADNVEVLAQSAEESSSSILEMAATND
EVAENIQALAASVEETTAAIEQMTYSIKEVAKNIEDLSATTEETSSSMNEMDVSISQVESNANETARLSEQAQRDAESGA
EALSRTLLGIDKIKESSKEAASVIEALGAKIAAIGNILNVIDDVAEQTNLLALNAAILAAQSGEHGKGFAVVADEIKDLA
ERTGASTKEISELIRAVQDQSRNAIQAMDRGVRNVEEGVRLGQETETALKKIQESSQKSTQMVKAIARATLEQARGSKQV
TMAINRIAETVQQIASATAEQARGSEQIMKSAERMKAITKHVERSAQEQARGSKQITRAIESISEMVHHLNRAQKEQTKG
SEQVMTAVVQIKQVAEAQTHSVRDLEAAIVDLASQAEVLRGEVRRFRI

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1788194, Length=278, Percent_Identity=31.294964028777, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1789453, Length=235, Percent_Identity=31.9148936170213, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788195, Length=267, Percent_Identity=30.3370786516854, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1787690, Length=250, Percent_Identity=32.4, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI2367378, Length=225, Percent_Identity=32.8888888888889, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 72988; Mature: 72988

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPKLAHLGVKRILFLSIAGALAIAGVLTSIAVNREIHVALERAAVHRGEADARVYASAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHH
APALAAGDPARIAARVDEDWDEESFAYVVVVREDWSVAARHLSRNLGRSAEEVVAWHQAA
HHHHHCCCHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHC
GGAAQPQAPGVLAFTRPVEGAGGGARAGYVLLGLDRDPLDGQAGRAGWYIFALLVGGGMV
CCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
LAGLVYVFVSRLVLRPLDEMSAVARRVSDCDLTARSAQLGPDELGTLAESLNRIGENLAA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TLSRVQNVTGGVATVIDRISRTGSAVSSGAGTVTARVVDTSSSMSQMISSLKGIADNVEV
HHHHHHHHCCHHHHHHHHHHHCCCHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHH
LAQSAEESSSSILEMAATNDEVAENIQALAASVEETTAAIEQMTYSIKEVAKNIEDLSAT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEETSSSMNEMDVSISQVESNANETARLSEQAQRDAESGAEALSRTLLGIDKIKESSKEA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASVIEALGAKIAAIGNILNVIDDVAEQTNLLALNAAILAAQSGEHGKGFAVVADEIKDLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
ERTGASTKEISELIRAVQDQSRNAIQAMDRGVRNVEEGVRLGQETETALKKIQESSQKST
HHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QMVKAIARATLEQARGSKQVTMAINRIAETVQQIASATAEQARGSEQIMKSAERMKAITK
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
HVERSAQEQARGSKQITRAIESISEMVHHLNRAQKEQTKGSEQVMTAVVQIKQVAEAQTH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SVRDLEAAIVDLASQAEVLRGEVRRFRI
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPKLAHLGVKRILFLSIAGALAIAGVLTSIAVNREIHVALERAAVHRGEADARVYASAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHH
APALAAGDPARIAARVDEDWDEESFAYVVVVREDWSVAARHLSRNLGRSAEEVVAWHQAA
HHHHHCCCHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHC
GGAAQPQAPGVLAFTRPVEGAGGGARAGYVLLGLDRDPLDGQAGRAGWYIFALLVGGGMV
CCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
LAGLVYVFVSRLVLRPLDEMSAVARRVSDCDLTARSAQLGPDELGTLAESLNRIGENLAA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TLSRVQNVTGGVATVIDRISRTGSAVSSGAGTVTARVVDTSSSMSQMISSLKGIADNVEV
HHHHHHHHCCHHHHHHHHHHHCCCHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHH
LAQSAEESSSSILEMAATNDEVAENIQALAASVEETTAAIEQMTYSIKEVAKNIEDLSAT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEETSSSMNEMDVSISQVESNANETARLSEQAQRDAESGAEALSRTLLGIDKIKESSKEA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASVIEALGAKIAAIGNILNVIDDVAEQTNLLALNAAILAAQSGEHGKGFAVVADEIKDLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
ERTGASTKEISELIRAVQDQSRNAIQAMDRGVRNVEEGVRLGQETETALKKIQESSQKST
HHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QMVKAIARATLEQARGSKQVTMAINRIAETVQQIASATAEQARGSEQIMKSAERMKAITK
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
HVERSAQEQARGSKQITRAIESISEMVHHLNRAQKEQTKGSEQVMTAVVQIKQVAEAQTH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SVRDLEAAIVDLASQAEVLRGEVRRFRI
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]