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The map label for this gene is dnaA [H]

Identifier: 220915124

GI number: 220915124

Start: 248

End: 1624

Strand: Direct

Name: dnaA [H]

Synonym: A2cp1_0001

Alternate gene names: 220915124

Gene position: 248-1624 (Clockwise)

Preceding gene: NA

Following gene: 220915125

Centisome position: 0.0

GC content: 70.23

Gene sequence:

>1377_bases
ATGGAAGCACCTGCGAGCGCACTCGTGGAAACGCCGCTCGACGATCTCTGGTCCCGCATCGACGGGTTCCTCCGCGCCCG
CTGCCGCGAGGACCTGTACGAGCGCTGGTTCGAGCCGCTCCGCCCCGTCTCGCTCGACGAGGGCCGGCTCCAGATCGGCG
CGCCGGACAAGTTCCACCGCGACTTCGTCGAGGACAACTACCGCGGCTGGTTCGAGGAGTTCATCCCCGAGCTGACCGGC
GAGCGCCTGCGGATCGCGTTCGCGGTGGACGATGCGCCGCGACCGATCGGCGCGGCAGCCGCCCCGGCGCCGGCCGCCGT
CGCGGCGGCGCGCGCGCCCGAGCCGCACCTCGACCCCCGCGACTCCCGCCCGAACCCGCGCTACGCCTTCGACACGTTCG
TGGTGGGCGCGTCCAACCACTTCGCGTTCGCCGCCGCCCAGGCCGTCGCCGCCAACCCCGGCCGCACCTGGAACCCGCTC
TTCCTGCACGGAGACTCCGGCCTCGGGAAGACCCACCTGCTGCACGCGGTGGCCCACGCGATCCTCGAGAAGAGCGGCGG
GCGCGTCGCGATCGTCTCCTCCGAGCGCTACACCAACGACTTCGTCGCCGCGCTCTCGAAGGGCACGATGGACGAGTTCC
GCCGCAAGTACCGCGAGTGCTCCGCGCTGCTGGTGGACGACGTCCAGTTCCTCGCCGGCAAGGACAAGACGGCGGAGGAG
TTCTTCCACACGTTCAACGAGCTCCACGACCACCACGTCCAGATCGTGCTCTCCAGCGATCGCTCGCCGAAGGAGCTGAA
GGGCCTCGACGAGCGGCTCTGCTCCCGCTTCGAGTGGGGCATGCGGGTGCAGATCGAGGTCCCGGAGTTCGAGACCCGCG
CCGCCATCCTCCAGAAGAAGGCGGACGTGGAGAAGATCGATCTGCCCGACGACGTCACCCAGCTCCTCGCGCAGCACATC
CGCTCGAACGTCCGCGAGCTGGAGGGCGCGCTCATGCGCCTCGCCGCGTTCGCCTCGCTGAAGAGCGAGCCGATCACGGT
GCCGCTCGCGCGCGACGTCCTCGCCGACGTCATCCCGCCGCCCGGCTACCGCCCGCCCATCGAGAAGATCCAGGAGGCCG
TGGCCGGCCACTACGGGCTCACCGTGGCGAAGCTCACCAGCGCCTCGCGCGAGCAGAAGATCGCGTTCCCGCGCCAGGTG
GCGATGTACCTCTGCCGCGAGCTGGCGAAGGAGAAGTTCCAGTCCATCGCCGAGAAGTTCAACAAGAAGGACCACACCAC
CGTCATCGCCGCGGTGGACCGCGTGAAGGGGCTGCTCGAGTCGGACGACGCCGTCCGCACCGCCGTGGCCCAGCTCTCCG
CCAAGCTCGCGCCGTGA

Upstream 100 bases:

>100_bases
CCGACTCCTCGCAGACCGAGCGAAGAGTGGAGCCGCAGACTCCCAGGAGCCTGCGGACGGGGGGCATTTTCGCGTTTTTT
CGGTCCTGGGGGACGTGGGG

Downstream 100 bases:

>100_bases
TCCACCCGCGCCCTCGCGCCACGCCCCGCCGCCTCGCGGCCGGGGACGGCCGCGGTGCGCCCGCGCTCCCCTCCGCCAGC
ACCCGGCCCGCGCGGGCGAC

Product: chromosomal replication initiator protein DnaA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MEAPASALVETPLDDLWSRIDGFLRARCREDLYERWFEPLRPVSLDEGRLQIGAPDKFHRDFVEDNYRGWFEEFIPELTG
ERLRIAFAVDDAPRPIGAAAAPAPAAVAAARAPEPHLDPRDSRPNPRYAFDTFVVGASNHFAFAAAQAVAANPGRTWNPL
FLHGDSGLGKTHLLHAVAHAILEKSGGRVAIVSSERYTNDFVAALSKGTMDEFRRKYRECSALLVDDVQFLAGKDKTAEE
FFHTFNELHDHHVQIVLSSDRSPKELKGLDERLCSRFEWGMRVQIEVPEFETRAAILQKKADVEKIDLPDDVTQLLAQHI
RSNVRELEGALMRLAAFASLKSEPITVPLARDVLADVIPPPGYRPPIEKIQEAVAGHYGLTVAKLTSASREQKIAFPRQV
AMYLCRELAKEKFQSIAEKFNKKDHTTVIAAVDRVKGLLESDDAVRTAVAQLSAKLAP

Sequences:

>Translated_458_residues
MEAPASALVETPLDDLWSRIDGFLRARCREDLYERWFEPLRPVSLDEGRLQIGAPDKFHRDFVEDNYRGWFEEFIPELTG
ERLRIAFAVDDAPRPIGAAAAPAPAAVAAARAPEPHLDPRDSRPNPRYAFDTFVVGASNHFAFAAAQAVAANPGRTWNPL
FLHGDSGLGKTHLLHAVAHAILEKSGGRVAIVSSERYTNDFVAALSKGTMDEFRRKYRECSALLVDDVQFLAGKDKTAEE
FFHTFNELHDHHVQIVLSSDRSPKELKGLDERLCSRFEWGMRVQIEVPEFETRAAILQKKADVEKIDLPDDVTQLLAQHI
RSNVRELEGALMRLAAFASLKSEPITVPLARDVLADVIPPPGYRPPIEKIQEAVAGHYGLTVAKLTSASREQKIAFPRQV
AMYLCRELAKEKFQSIAEKFNKKDHTTVIAAVDRVKGLLESDDAVRTAVAQLSAKLAP
>Mature_458_residues
MEAPASALVETPLDDLWSRIDGFLRARCREDLYERWFEPLRPVSLDEGRLQIGAPDKFHRDFVEDNYRGWFEEFIPELTG
ERLRIAFAVDDAPRPIGAAAAPAPAAVAAARAPEPHLDPRDSRPNPRYAFDTFVVGASNHFAFAAAQAVAANPGRTWNPL
FLHGDSGLGKTHLLHAVAHAILEKSGGRVAIVSSERYTNDFVAALSKGTMDEFRRKYRECSALLVDDVQFLAGKDKTAEE
FFHTFNELHDHHVQIVLSSDRSPKELKGLDERLCSRFEWGMRVQIEVPEFETRAAILQKKADVEKIDLPDDVTQLLAQHI
RSNVRELEGALMRLAAFASLKSEPITVPLARDVLADVIPPPGYRPPIEKIQEAVAGHYGLTVAKLTSASREQKIAFPRQV
AMYLCRELAKEKFQSIAEKFNKKDHTTVIAAVDRVKGLLESDDAVRTAVAQLSAKLAP

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=463, Percent_Identity=39.524838012959, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI226510964, Length=228, Percent_Identity=26.3157894736842, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 51027; Mature: 51027

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAPASALVETPLDDLWSRIDGFLRARCREDLYERWFEPLRPVSLDEGRLQIGAPDKFHR
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHH
DFVEDNYRGWFEEFIPELTGERLRIAFAVDDAPRPIGAAAAPAPAAVAAARAPEPHLDPR
HHHHCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
DSRPNPRYAFDTFVVGASNHFAFAAAQAVAANPGRTWNPLFLHGDSGLGKTHLLHAVAHA
CCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHH
ILEKSGGRVAIVSSERYTNDFVAALSKGTMDEFRRKYRECSALLVDDVQFLAGKDKTAEE
HHHCCCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FFHTFNELHDHHVQIVLSSDRSPKELKGLDERLCSRFEWGMRVQIEVPEFETRAAILQKK
HHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
ADVEKIDLPDDVTQLLAQHIRSNVRELEGALMRLAAFASLKSEPITVPLARDVLADVIPP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCC
PGYRPPIEKIQEAVAGHYGLTVAKLTSASREQKIAFPRQVAMYLCRELAKEKFQSIAEKF
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
NKKDHTTVIAAVDRVKGLLESDDAVRTAVAQLSAKLAP
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEAPASALVETPLDDLWSRIDGFLRARCREDLYERWFEPLRPVSLDEGRLQIGAPDKFHR
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHH
DFVEDNYRGWFEEFIPELTGERLRIAFAVDDAPRPIGAAAAPAPAAVAAARAPEPHLDPR
HHHHCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
DSRPNPRYAFDTFVVGASNHFAFAAAQAVAANPGRTWNPLFLHGDSGLGKTHLLHAVAHA
CCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHH
ILEKSGGRVAIVSSERYTNDFVAALSKGTMDEFRRKYRECSALLVDDVQFLAGKDKTAEE
HHHCCCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FFHTFNELHDHHVQIVLSSDRSPKELKGLDERLCSRFEWGMRVQIEVPEFETRAAILQKK
HHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
ADVEKIDLPDDVTQLLAQHIRSNVRELEGALMRLAAFASLKSEPITVPLARDVLADVIPP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCC
PGYRPPIEKIQEAVAGHYGLTVAKLTSASREQKIAFPRQVAMYLCRELAKEKFQSIAEKF
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
NKKDHTTVIAAVDRVKGLLESDDAVRTAVAQLSAKLAP
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA