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The map label for this gene is livJ [H]

Identifier: 220914815

GI number: 220914815

Start: 176048

End: 177202

Strand: Reverse

Name: livJ [H]

Synonym: Mnod_7952

Alternate gene names: 220914815

Gene position: 177202-176048 (Counterclockwise)

Preceding gene: 220914816

Following gene: 220914814

Centisome position: 38.68

GC content: 67.88

Gene sequence:

>1155_bases
ATGAGACAGAGCGCGCCATCCGTCAGCCGCCGGGCCTTCCTGGCGGCATCGCTCGCGGCGGCGGCCATCGGGCCCGCTCG
GGCCGAGGAGGCGCTGCGCTTCGGCATGGCGATGCCCCTGTCGGGCGGTCAGGCGACCTACGGCCAGGACCAGGTCAAGG
CGGCCGAGTGGGCCGTGGCGGCCATCAACGAGAGGGGCGGGGTCGCCGGCCGCAAGCTCGAGATGATCGTGCTCGACACC
CGCGCGGATCCGCAGGCGGGCATTCAGGCCGCGAACCGGCTGATCTCGGTCGACAAGGTGCCGGTCTTCGTCAGCGCGTG
GTCGGCGGTGGTCAAGGCAGTGGCCCCCGTGGCGAATGACAGCCGCACGGTGCAACTCTCGGTCGGCGCGAATTCCGCCG
AGATCGCCCGGCTCGGGGATTACACCTACACCACCTTCCCGCTCGCCTCGGTCGACATCACGGCGGTGGCGACCTACGCC
GCCAAGCAGATGGGCAAGAAGCGGGCGGCTGTCCTCTACATCAACAACGAGACCGGGACGGTCGCGGCGCAGATCTACCG
CGACGTCTTCGCCAAGTCCGGCGGGCAGGTCGTGGCCTACGAGGCCTATGATCCGCGCGCGTCGGATTGGACCGGACCGC
TGCTCAAGGTCCGGGCCGCGCAGCCCGACATCGTGCATATCCAGGGCCTCGTCGCCGATACGCCGCAGGTGATCGCGCAG
ATGCGCCAGCTCGGCCTCACGCTGCCGGTCTCGTCCTATTCGGCGGTCTACAATCCGAAGCTGATCGAGCAGCTCGGCAA
GGCCGCCGAGGGCGTCATCGCCACCTCGCTGGCGCCGGGCGTCACCGACAGTCCCGCCGTGGCGGCCTATGTCGAGCGCT
GGAAGAAGGAAGTCGGCCGGGAGCCGAACGGCCTGCCCTATACCCAGTATCTCTACGATGCGCCTTACATCGTGGCCGAG
GTCTACCGGTCGCTGATCGAGAAGAAGATCCCGCTCTCCGGCGAGAACTTCCGCAAGGAAATGCTGGCGATCCGCAGCTT
CGATCTGCCGCTCACCGGCAAGCTCGTCATCAACGACGACCACACGGTGAACAAGCCCGTCTTCCTGATGGAAGTGAAGG
GCGGGCGCTGGGTGCAGAAGGCGGTGGTCGAGTAG

Upstream 100 bases:

>100_bases
GAAGCCCTGCGCGCGCTGGTCTGACGACCGCCGCCCGAGAGAACCCCTGCGGCGCGGCTTCGCCGCCGGGAAGCGACACC
GCCAGAGAGAGAGAGCGACC

Downstream 100 bases:

>100_bases
GGCGCGCTCTGCCGTCCGGGAAGGCGAGGAAACCGATGCTGACCTTCGACATCCTCGCGCAGGTCTTGTGGACGTCGTTC
GCGACGTCCACCTACCTCGT

Product: Extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-BP [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MRQSAPSVSRRAFLAASLAAAAIGPARAEEALRFGMAMPLSGGQATYGQDQVKAAEWAVAAINERGGVAGRKLEMIVLDT
RADPQAGIQAANRLISVDKVPVFVSAWSAVVKAVAPVANDSRTVQLSVGANSAEIARLGDYTYTTFPLASVDITAVATYA
AKQMGKKRAAVLYINNETGTVAAQIYRDVFAKSGGQVVAYEAYDPRASDWTGPLLKVRAAQPDIVHIQGLVADTPQVIAQ
MRQLGLTLPVSSYSAVYNPKLIEQLGKAAEGVIATSLAPGVTDSPAVAAYVERWKKEVGREPNGLPYTQYLYDAPYIVAE
VYRSLIEKKIPLSGENFRKEMLAIRSFDLPLTGKLVINDDHTVNKPVFLMEVKGGRWVQKAVVE

Sequences:

>Translated_384_residues
MRQSAPSVSRRAFLAASLAAAAIGPARAEEALRFGMAMPLSGGQATYGQDQVKAAEWAVAAINERGGVAGRKLEMIVLDT
RADPQAGIQAANRLISVDKVPVFVSAWSAVVKAVAPVANDSRTVQLSVGANSAEIARLGDYTYTTFPLASVDITAVATYA
AKQMGKKRAAVLYINNETGTVAAQIYRDVFAKSGGQVVAYEAYDPRASDWTGPLLKVRAAQPDIVHIQGLVADTPQVIAQ
MRQLGLTLPVSSYSAVYNPKLIEQLGKAAEGVIATSLAPGVTDSPAVAAYVERWKKEVGREPNGLPYTQYLYDAPYIVAE
VYRSLIEKKIPLSGENFRKEMLAIRSFDLPLTGKLVINDDHTVNKPVFLMEVKGGRWVQKAVVE
>Mature_384_residues
MRQSAPSVSRRAFLAASLAAAAIGPARAEEALRFGMAMPLSGGQATYGQDQVKAAEWAVAAINERGGVAGRKLEMIVLDT
RADPQAGIQAANRLISVDKVPVFVSAWSAVVKAVAPVANDSRTVQLSVGANSAEIARLGDYTYTTFPLASVDITAVATYA
AKQMGKKRAAVLYINNETGTVAAQIYRDVFAKSGGQVVAYEAYDPRASDWTGPLLKVRAAQPDIVHIQGLVADTPQVIAQ
MRQLGLTLPVSSYSAVYNPKLIEQLGKAAEGVIATSLAPGVTDSPAVAAYVERWKKEVGREPNGLPYTQYLYDAPYIVAE
VYRSLIEKKIPLSGENFRKEMLAIRSFDLPLTGKLVINDDHTVNKPVFLMEVKGGRWVQKAVVE

Specific function: This protein is a component of the leucine, isoleucine, valine, (threonine) transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=270, Percent_Identity=24.8148148148148, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789867, Length=302, Percent_Identity=23.1788079470199, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41208; Mature: 41208

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQSAPSVSRRAFLAASLAAAAIGPARAEEALRFGMAMPLSGGQATYGQDQVKAAEWAVA
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEE
AINERGGVAGRKLEMIVLDTRADPQAGIQAANRLISVDKVPVFVSAWSAVVKAVAPVAND
EECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCC
SRTVQLSVGANSAEIARLGDYTYTTFPLASVDITAVATYAAKQMGKKRAAVLYINNETGT
CEEEEEEECCCCHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCH
VAAQIYRDVFAKSGGQVVAYEAYDPRASDWTGPLLKVRAAQPDIVHIQGLVADTPQVIAQ
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCHHHHHH
MRQLGLTLPVSSYSAVYNPKLIEQLGKAAEGVIATSLAPGVTDSPAVAAYVERWKKEVGR
HHHCCCCCCCCCCHHHCCHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHCC
EPNGLPYTQYLYDAPYIVAEVYRSLIEKKIPLSGENFRKEMLAIRSFDLPLTGKLVINDD
CCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCC
HTVNKPVFLMEVKGGRWVQKAVVE
CCCCCCEEEEEECCCCHHHHHHCC
>Mature Secondary Structure
MRQSAPSVSRRAFLAASLAAAAIGPARAEEALRFGMAMPLSGGQATYGQDQVKAAEWAVA
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEE
AINERGGVAGRKLEMIVLDTRADPQAGIQAANRLISVDKVPVFVSAWSAVVKAVAPVAND
EECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCC
SRTVQLSVGANSAEIARLGDYTYTTFPLASVDITAVATYAAKQMGKKRAAVLYINNETGT
CEEEEEEECCCCHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCH
VAAQIYRDVFAKSGGQVVAYEAYDPRASDWTGPLLKVRAAQPDIVHIQGLVADTPQVIAQ
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCHHHHHH
MRQLGLTLPVSSYSAVYNPKLIEQLGKAAEGVIATSLAPGVTDSPAVAAYVERWKKEVGR
HHHCCCCCCCCCCHHHCCHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHCC
EPNGLPYTQYLYDAPYIVAEVYRSLIEKKIPLSGENFRKEMLAIRSFDLPLTGKLVINDD
CCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCC
HTVNKPVFLMEVKGGRWVQKAVVE
CCCCCCEEEEEECCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA