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The map label for this gene is proI [H]

Identifier: 220914768

GI number: 220914768

Start: 121794

End: 122624

Strand: Reverse

Name: proI [H]

Synonym: Mnod_7890

Alternate gene names: 220914768

Gene position: 122624-121794 (Counterclockwise)

Preceding gene: 220914773

Following gene: 220914767

Centisome position: 26.77

GC content: 55.96

Gene sequence:

>831_bases
GTGAAAACCACCCTCGTGCTGTTTGGTGTTGGCCACATGGGTTCGGCCATCGCCCGCGGAATGTTGCAGACCGGAAACAC
TGATCTTCGAATCATTGACCCAGATCTCAGCGCGCTTCAAGAGTTCAGTGCGCTCGGAATCCGCACTGATACTGCTTGTT
TAAGTTTGGCACCGAATGACGTGCTCATGTTGGCTATGCCGCCGCAGGTCTTTGGCGCTTTCTCATCGGACACTCCATGC
GTACATCATCATCAGGGGTTGGTCATTTCTGTGATGGCCGGCATTCGGATCAGCAGCATCCAGCAGATGCTGGGTACTCA
GAACGTAGTCCGCTCAATTCCCAATACTCCTTCGGAGGTCTTCGAAGGGATGACTGTCTACTGCCGGTCTTCATCGGTAG
GCGAGAACCTCGCTCGAAAGGCACGGGACGTCCTTGAATCGTTCGGTCGAGCAGTCGAAGTCAGTGACGAGGCGCTGATA
GATCCTGCCACTGCCCTGTGTGGGGGAGGTCCGGCATTCGTCGCCTACTTTGCCGACGCATTGCAAGAGTTTGGCATCAA
GGCTGGTCTTTCAAAGGATGCCGCGCTTGCAATCACGATTCAGCTTCTGCGGGGCACTTCAGCACTGTTGGCGACCACAG
GCAAACCTGTGATGCAACTTTGCCGCGAGGTCATGACACCCGGTGGTACGACCGAGCGGGGTATCTGGCACTTTGACGAC
GAAGGCGTTGGCCGTACGGTCCTGGACGCTCTTTCCAAGTCATCCGCACGTTCGCGCGAGCTTGGGCTCGCAATTACTGA
GTCCCTTTCTCAGTTCGAGGTGGCAAAATGA

Upstream 100 bases:

>100_bases
AAGCCGGCTGAGGTGTTGCCATGAGTCAAGATTCATCAAGTGGGTGATGTGGTGCACCGTTACTCATGCCTTCAACAACG
CACTCATGGGGAGAGTTGCA

Downstream 100 bases:

>100_bases
TCGGAGTAAGCGACAAGAGTTGGCTGGTCGACCTACTGTCGACCGTTGCCCCAGACAGACCGGATCTAGTGCCGGAAATT
GCTGCGGACAGCGACAAGCA

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase 2; P5CR 2 [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKTTLVLFGVGHMGSAIARGMLQTGNTDLRIIDPDLSALQEFSALGIRTDTACLSLAPNDVLMLAMPPQVFGAFSSDTPC
VHHHQGLVISVMAGIRISSIQQMLGTQNVVRSIPNTPSEVFEGMTVYCRSSSVGENLARKARDVLESFGRAVEVSDEALI
DPATALCGGGPAFVAYFADALQEFGIKAGLSKDAALAITIQLLRGTSALLATTGKPVMQLCREVMTPGGTTERGIWHFDD
EGVGRTVLDALSKSSARSRELGLAITESLSQFEVAK

Sequences:

>Translated_276_residues
MKTTLVLFGVGHMGSAIARGMLQTGNTDLRIIDPDLSALQEFSALGIRTDTACLSLAPNDVLMLAMPPQVFGAFSSDTPC
VHHHQGLVISVMAGIRISSIQQMLGTQNVVRSIPNTPSEVFEGMTVYCRSSSVGENLARKARDVLESFGRAVEVSDEALI
DPATALCGGGPAFVAYFADALQEFGIKAGLSKDAALAITIQLLRGTSALLATTGKPVMQLCREVMTPGGTTERGIWHFDD
EGVGRTVLDALSKSSARSRELGLAITESLSQFEVAK
>Mature_276_residues
MKTTLVLFGVGHMGSAIARGMLQTGNTDLRIIDPDLSALQEFSALGIRTDTACLSLAPNDVLMLAMPPQVFGAFSSDTPC
VHHHQGLVISVMAGIRISSIQQMLGTQNVVRSIPNTPSEVFEGMTVYCRSSSVGENLARKARDVLESFGRAVEVSDEALI
DPATALCGGGPAFVAYFADALQEFGIKAGLSKDAALAITIQLLRGTSALLATTGKPVMQLCREVMTPGGTTERGIWHFDD
EGVGRTVLDALSKSSARSRELGLAITESLSQFEVAK

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family [H]

Homologues:

Organism=Homo sapiens, GI198041662, Length=260, Percent_Identity=33.0769230769231, Blast_Score=121, Evalue=5e-28,
Organism=Homo sapiens, GI24797095, Length=281, Percent_Identity=29.8932384341637, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI24797097, Length=281, Percent_Identity=29.8932384341637, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI21361454, Length=267, Percent_Identity=29.2134831460674, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1786585, Length=269, Percent_Identity=25.6505576208178, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17569021, Length=264, Percent_Identity=32.5757575757576, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17540664, Length=287, Percent_Identity=24.390243902439, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320861, Length=176, Percent_Identity=30.1136363636364, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648116, Length=276, Percent_Identity=31.1594202898551, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21358587, Length=265, Percent_Identity=30.188679245283, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24647700, Length=169, Percent_Identity=30.1775147928994, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304 [H]

Pfam domain/function: PF03807 F420_oxidored [H]

EC number: =1.5.1.2 [H]

Molecular weight: Translated: 29201; Mature: 29201

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTLVLFGVGHMGSAIARGMLQTGNTDLRIIDPDLSALQEFSALGIRTDTACLSLAPND
CCCEEEEEECCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCEEEECCCC
VLMLAMPPQVFGAFSSDTPCVHHHQGLVISVMAGIRISSIQQMLGTQNVVRSIPNTPSEV
EEEEEECHHHHHCCCCCCCCEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHH
FEGMTVYCRSSSVGENLARKARDVLESFGRAVEVSDEALIDPATALCGGGPAFVAYFADA
HCCCEEEECCCCHHHHHHHHHHHHHHHHCCEEECCCHHHHCCHHHHHCCCHHHHHHHHHH
LQEFGIKAGLSKDAALAITIQLLRGTSALLATTGKPVMQLCREVMTPGGTTERGIWHFDD
HHHCCHHCCCCCCCCCEEEEHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEECC
EGVGRTVLDALSKSSARSRELGLAITESLSQFEVAK
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTTLVLFGVGHMGSAIARGMLQTGNTDLRIIDPDLSALQEFSALGIRTDTACLSLAPND
CCCEEEEEECCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCEEEECCCC
VLMLAMPPQVFGAFSSDTPCVHHHQGLVISVMAGIRISSIQQMLGTQNVVRSIPNTPSEV
EEEEEECHHHHHCCCCCCCCEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHH
FEGMTVYCRSSSVGENLARKARDVLESFGRAVEVSDEALIDPATALCGGGPAFVAYFADA
HCCCEEEECCCCHHHHHHHHHHHHHHHHCCEEECCCHHHHCCHHHHHCCCHHHHHHHHHH
LQEFGIKAGLSKDAALAITIQLLRGTSALLATTGKPVMQLCREVMTPGGTTERGIWHFDD
HHHCCHHCCCCCCCCCEEEEHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEECC
EGVGRTVLDALSKSSARSRELGLAITESLSQFEVAK
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 11418582 [H]