| Definition | Methylobacterium nodulans ORS 2060 plasmid pMNOD02, complete sequence. |
|---|---|
| Accession | NC_011887 |
| Length | 458,070 |
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The map label for this gene is iolD [H]
Identifier: 220914694
GI number: 220914694
Start: 31016
End: 32854
Strand: Reverse
Name: iolD [H]
Synonym: Mnod_7802
Alternate gene names: 220914694
Gene position: 32854-31016 (Counterclockwise)
Preceding gene: 220914695
Following gene: 220914693
Centisome position: 7.17
GC content: 68.46
Gene sequence:
>1839_bases ATGATGACGACGGTGCGGTTGACGGCGGCGCAGACCATGATGCGCTGGCTTTCGGTGCAGATGACCGAAGAAGGCGAGCG GTTCATCGAGGGGGTCTGGGCGATCTTCGGCCATGGCAACGTGGCCGGAATCGGCGAGGCGCTGGAGAAGATCGGCGACG CATTGCCCACCTGGCGCGGCCAGAACGAGCAGACGATGGCCCATGCGGCCATCGCCTATGCCAAGACCAAGCGCCGCCGG AAGGCGATGGCCGTCACCTCGTCGATCGGGCCGGGGGCCACCAACATGGTGACGGCGGCGGCACTGGCGCATGTGAACCG CCTGCCGGTGCTGTTGATCCCGGGCGATGTCTTCGCCAACCGGCGCCCCGATCCGGTGCTGCAGCAGATCGAGGATTTCG ACGACGGCACGGTCTCGGCCAATGACTGCTTCCGGCCGGTCAGCCGCTATTTCGACCGGATCACCCGGCCAGAGCACCTG CTGACGGCGTTGCCGCGCGCCCTGCAGGTGATGACCGACCCGGCCCAGTGCGGGCCGGTGACGCTGGCCTTCTGCCAGGA CGTGCAGGCCGAGGCGTACGACTGGCCCGAGGCGTTCTTCGCGCCGAAGGTCTGGCACATCCGTCGCCCGGAACCGGACC GGCGCGAGCTCGCGGCCGTGATCGAGATGATCCGCGCGGCGCAGAAGCCGGTGATCGTGTCGGGCGGGGGCGTGCTCTAT TCCGGGGCCGATCAGGACCTCGCCGCCTTCGCGGAAAAGCACAACATCCCGGTGGTCGAGACGCAGGCCGGCAAGGGCGC GCTCGACTGGCAGCACCCGCTCAACTTCGGCTCTCCCGGCGTGACGGGCAGCGACTGCGCCAACCGGATCTGTGCCGAGG CCGACCTGATCATCGGCGCGGGCACGCGCTTCCAGGACTTCACCACCGGCAGCTGGGCCGCCTTCCGCAACCCTCAGCGC AAACTCGTTTCGGTCAACCTGACCGGTTATGACGCCGCCAAGCACAGTGCGGTGCCGTTAGTTGCCGATGCGAAGATCGC GCTCACGGCGATCACCGCCGGGCTGGAAGGTCACCGGTTCGCGGGGCCGGATGCGAGCCTGCGCACGGCCTGGTTTAAGG CGGCCGACCGGGTGATGGCGGCGCCGCAGGGCGAGAACCCGAACGATCTGCCCACCGACGCGCAGGTAGTCGGGGCGGTG CAGCGCTATGCGGGCGCCGACACAGTGGTGATGTGCGCGGCCGGCACCATGCCCGGCGCGCTGCAGGTGCTGTGGCGCGC GGCACCCGGCGGCTACCACATGGAATACGGCTATTCCTGCATGGGCTACGAGGTGGCCGGCGCCATGGGCATCGCGCTGG CCGTGCCCGAGAAGGAAGTCATCTGCTTCGTGGGCGATGGCAGCTACATGATGGCCAATTCCGAACTGGCCACGGCGGCG ATGATGCATGTGCCCTTCACCATCGTGCTGACGGACAATCGCGGTTATGGCTGCATCAACCGGCTGCAGATCGAGTGCGG CGGCGCGCCCTTCAACAACATGTATGTGGACAGCCGCCACAAGACCTTCCCCGAGATCGATTTCGTCGCGCACGCCCGCG CGATGGGCGCCGAGGCGGTGAAGGTCGCCGGCATTGCCGGGCTGGAGGCGGAACTGGCCCGGCCCCGTGACCGCAAGGTC CCGCGCGTGATCCTGATCGACACCGAGGCCGAGAGCAGCCTCGGCGTCGGTGGGGCCTGGTGGGACGTGGCCGTGCCCGA AGTGGGGACAACCGAAGCCCTGCGCGCGGCCCGCGAACGCTACGACAGCCATGCCGCCTTCCAGCGGACGTTCGACTGA
Upstream 100 bases:
>100_bases AGGACGCGGTTGCGGAGATGGTGCGCCGCTATGCGCGGCTTTGCGCAGTCTGGGATGCCGGGAAGGGCCTTGCGAGTGCA ACGGCCGGACGGGAGCAGGC
Downstream 100 bases:
>100_bases CGGGATCGGGGCGCCCCGACGCGTCGGGCCCATCCGGAACGAGGCCGGACGAGCGCAGGCGCACTGGTCCAAGCCGTGGC CGACGGAGATCATATGATGG
Product: thiamine pyrophosphate protein central region
Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]
Alternate protein names: THcHDO hydrolase [H]
Number of amino acids: Translated: 612; Mature: 612
Protein sequence:
>612_residues MMTTVRLTAAQTMMRWLSVQMTEEGERFIEGVWAIFGHGNVAGIGEALEKIGDALPTWRGQNEQTMAHAAIAYAKTKRRR KAMAVTSSIGPGATNMVTAAALAHVNRLPVLLIPGDVFANRRPDPVLQQIEDFDDGTVSANDCFRPVSRYFDRITRPEHL LTALPRALQVMTDPAQCGPVTLAFCQDVQAEAYDWPEAFFAPKVWHIRRPEPDRRELAAVIEMIRAAQKPVIVSGGGVLY SGADQDLAAFAEKHNIPVVETQAGKGALDWQHPLNFGSPGVTGSDCANRICAEADLIIGAGTRFQDFTTGSWAAFRNPQR KLVSVNLTGYDAAKHSAVPLVADAKIALTAITAGLEGHRFAGPDASLRTAWFKAADRVMAAPQGENPNDLPTDAQVVGAV QRYAGADTVVMCAAGTMPGALQVLWRAAPGGYHMEYGYSCMGYEVAGAMGIALAVPEKEVICFVGDGSYMMANSELATAA MMHVPFTIVLTDNRGYGCINRLQIECGGAPFNNMYVDSRHKTFPEIDFVAHARAMGAEAVKVAGIAGLEAELARPRDRKV PRVILIDTEAESSLGVGGAWWDVAVPEVGTTEALRAARERYDSHAAFQRTFD
Sequences:
>Translated_612_residues MMTTVRLTAAQTMMRWLSVQMTEEGERFIEGVWAIFGHGNVAGIGEALEKIGDALPTWRGQNEQTMAHAAIAYAKTKRRR KAMAVTSSIGPGATNMVTAAALAHVNRLPVLLIPGDVFANRRPDPVLQQIEDFDDGTVSANDCFRPVSRYFDRITRPEHL LTALPRALQVMTDPAQCGPVTLAFCQDVQAEAYDWPEAFFAPKVWHIRRPEPDRRELAAVIEMIRAAQKPVIVSGGGVLY SGADQDLAAFAEKHNIPVVETQAGKGALDWQHPLNFGSPGVTGSDCANRICAEADLIIGAGTRFQDFTTGSWAAFRNPQR KLVSVNLTGYDAAKHSAVPLVADAKIALTAITAGLEGHRFAGPDASLRTAWFKAADRVMAAPQGENPNDLPTDAQVVGAV QRYAGADTVVMCAAGTMPGALQVLWRAAPGGYHMEYGYSCMGYEVAGAMGIALAVPEKEVICFVGDGSYMMANSELATAA MMHVPFTIVLTDNRGYGCINRLQIECGGAPFNNMYVDSRHKTFPEIDFVAHARAMGAEAVKVAGIAGLEAELARPRDRKV PRVILIDTEAESSLGVGGAWWDVAVPEVGTTEALRAARERYDSHAAFQRTFD >Mature_612_residues MMTTVRLTAAQTMMRWLSVQMTEEGERFIEGVWAIFGHGNVAGIGEALEKIGDALPTWRGQNEQTMAHAAIAYAKTKRRR KAMAVTSSIGPGATNMVTAAALAHVNRLPVLLIPGDVFANRRPDPVLQQIEDFDDGTVSANDCFRPVSRYFDRITRPEHL LTALPRALQVMTDPAQCGPVTLAFCQDVQAEAYDWPEAFFAPKVWHIRRPEPDRRELAAVIEMIRAAQKPVIVSGGGVLY SGADQDLAAFAEKHNIPVVETQAGKGALDWQHPLNFGSPGVTGSDCANRICAEADLIIGAGTRFQDFTTGSWAAFRNPQR KLVSVNLTGYDAAKHSAVPLVADAKIALTAITAGLEGHRFAGPDASLRTAWFKAADRVMAAPQGENPNDLPTDAQVVGAV QRYAGADTVVMCAAGTMPGALQVLWRAAPGGYHMEYGYSCMGYEVAGAMGIALAVPEKEVICFVGDGSYMMANSELATAA MMHVPFTIVLTDNRGYGCINRLQIECGGAPFNNMYVDSRHKTFPEIDFVAHARAMGAEAVKVAGIAGLEAELARPRDRKV PRVILIDTEAESSLGVGGAWWDVAVPEVGTTEALRAARERYDSHAAFQRTFD
Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]
COG id: COG3962
COG function: function code E; Acetolactate synthase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the TPP enzyme family [H]
Homologues:
Organism=Escherichia coli, GI87081685, Length=559, Percent_Identity=25.5813953488372, Blast_Score=152, Evalue=8e-38, Organism=Escherichia coli, GI1790104, Length=592, Percent_Identity=26.6891891891892, Blast_Score=139, Evalue=7e-34, Organism=Escherichia coli, GI1786717, Length=532, Percent_Identity=25.187969924812, Blast_Score=120, Evalue=3e-28, Organism=Escherichia coli, GI1787096, Length=572, Percent_Identity=21.6783216783217, Blast_Score=94, Evalue=3e-20, Organism=Escherichia coli, GI1788716, Length=437, Percent_Identity=23.7986270022883, Blast_Score=81, Evalue=2e-16, Organism=Saccharomyces cerevisiae, GI6323755, Length=595, Percent_Identity=25.2100840336134, Blast_Score=115, Evalue=3e-26,
Paralogues:
None
Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012000 - InterPro: IPR012001 - InterPro: IPR000399 - InterPro: IPR011766 [H]
Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]
EC number: 2.2.1.6 [C]
Molecular weight: Translated: 66018; Mature: 66018
Theoretical pI: Translated: 6.04; Mature: 6.04
Prosite motif: PS00187 TPP_ENZYMES
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 3.6 %Met (Translated Protein) 5.2 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 3.6 %Met (Mature Protein) 5.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MMTTVRLTAAQTMMRWLSVQMTEEGERFIEGVWAIFGHGNVAGIGEALEKIGDALPTWRG CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC QNEQTMAHAAIAYAKTKRRRKAMAVTSSIGPGATNMVTAAALAHVNRLPVLLIPGDVFAN CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHCC RRPDPVLQQIEDFDDGTVSANDCFRPVSRYFDRITRPEHLLTALPRALQVMTDPAQCGPV CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCE TLAFCQDVQAEAYDWPEAFFAPKVWHIRRPEPDRRELAAVIEMIRAAQKPVIVSGGGVLY EEEHHHCCCCCCCCCCHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEE SGADQDLAAFAEKHNIPVVETQAGKGALDWQHPLNFGSPGVTGSDCANRICAEADLIIGA CCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEC GTRFQDFTTGSWAAFRNPQRKLVSVNLTGYDAAKHSAVPLVADAKIALTAITAGLEGHRF CCCCCCCCCCCCHHHCCCCCEEEEEEECCCCCCCCCCCCEEECCCEEEEEEECCCCCCCC AGPDASLRTAWFKAADRVMAAPQGENPNDLPTDAQVVGAVQRYAGADTVVMCAAGTMPGA CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHH LQVLWRAAPGGYHMEYGYSCMGYEVAGAMGIALAVPEKEVICFVGDGSYMMANSELATAA HHHHHHCCCCCEEEECCCEEECEEHHCCCEEEEECCCCEEEEEECCCCEEEECCHHHHHH MMHVPFTIVLTDNRGYGCINRLQIECGGAPFNNMYVDSRHKTFPEIDFVAHARAMGAEAV HHHCCEEEEEECCCCCCCEEEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCE KVAGIAGLEAELARPRDRKVPRVILIDTEAESSLGVGGAWWDVAVPEVGTTEALRAARER EEECCCCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH YDSHAAFQRTFD HHHHHHHHHCCC >Mature Secondary Structure MMTTVRLTAAQTMMRWLSVQMTEEGERFIEGVWAIFGHGNVAGIGEALEKIGDALPTWRG CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC QNEQTMAHAAIAYAKTKRRRKAMAVTSSIGPGATNMVTAAALAHVNRLPVLLIPGDVFAN CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHCC RRPDPVLQQIEDFDDGTVSANDCFRPVSRYFDRITRPEHLLTALPRALQVMTDPAQCGPV CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCE TLAFCQDVQAEAYDWPEAFFAPKVWHIRRPEPDRRELAAVIEMIRAAQKPVIVSGGGVLY EEEHHHCCCCCCCCCCHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEE SGADQDLAAFAEKHNIPVVETQAGKGALDWQHPLNFGSPGVTGSDCANRICAEADLIIGA CCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEC GTRFQDFTTGSWAAFRNPQRKLVSVNLTGYDAAKHSAVPLVADAKIALTAITAGLEGHRF CCCCCCCCCCCCHHHCCCCCEEEEEEECCCCCCCCCCCCEEECCCEEEEEEECCCCCCCC AGPDASLRTAWFKAADRVMAAPQGENPNDLPTDAQVVGAVQRYAGADTVVMCAAGTMPGA CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHH LQVLWRAAPGGYHMEYGYSCMGYEVAGAMGIALAVPEKEVICFVGDGSYMMANSELATAA HHHHHHCCCCCEEEECCCEEECEEHHCCCEEEEECCCCEEEEEECCCCEEEECCHHHHHH MMHVPFTIVLTDNRGYGCINRLQIECGGAPFNNMYVDSRHKTFPEIDFVAHARAMGAEAV HHHCCEEEEEECCCCCCCEEEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCE KVAGIAGLEAELARPRDRKVPRVILIDTEAESSLGVGGAWWDVAVPEVGTTEALRAARER EEECCCCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH YDSHAAFQRTFD HHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: FAD [C]
Metal ions: Mg2+ [C]
Kcat value (1/min): 4002 [C]
Specific activity: 53
Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]
Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]
Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]
General reaction: C-C bond formation; Decarboxylation [C]
Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA