Click here to switch to the map view.

The map label for this gene is cls2 [H]

Identifier: 220905620

GI number: 220905620

Start: 2841201

End: 2842619

Strand: Reverse

Name: cls2 [H]

Synonym: Ddes_2360

Alternate gene names: 220905620

Gene position: 2842619-2841201 (Counterclockwise)

Preceding gene: 220905621

Following gene: 220905619

Centisome position: 98.93

GC content: 56.8

Gene sequence:

>1419_bases
ATGATTATCGGAGTGTTGGAAACACTGGTTCTCATAGGTGTGCATGTGCTGTCCTGGGTTGCGGTTTGTCATGCCCTGCT
TACCAAGCATGACCCGCGGGCGGCTTTGGGCTGGACGGTGACGGCCCTGCTGTTGCCCCTTGTGGGACCGTTGCTGTACG
CCACATTCGGCATCAGCAGGGCAGAAAGTCATGCCAGCCGCATGATGCGTCAGCAGCAACCTCTTGAGCCGGATTATGCT
CACCCTCCTTTTCCCGAACTGCCGCCGGAGGACGTGCCCGAGTACATCATCAGCATGGAGCGCATCGGCCGCCGCCTGAC
AGAGCAGCACCTGTGCAGTGGTAATTCCCTGACTCCTCTGCACAATGGCGACGAAGCCTATCCTGCCATGCTCAAGGCCA
TACGCGAGGCCAAGGAGCAGGTTTTTCTCTGCACCTACATCTTTAATGCAGGCAAAACCGCTACGGATTTCGGCAATGCC
ATGGCCGAAGCCGCAGAGCGCGGGGTTGACGTGCGCTTGCTGGTGGACGGTATCGGCATGCTTTACTCCTGGCGCAAGCC
CTGGAAAAAACTGGCTGAGCGTGGAGTAAACGTAGCCATGTTTCTTCCGCCGCGCCTGTTTCCGCCAAATTTCAGCATCA
ATCTGCGCAACCACCGCAAGATGCTTGTCTGCGACAATACTGCCTTTACCGGTGGCATGAATATTTCAGACGACAATATT
TCCGCCGGAAAGACCAAGTATGTACAGGACGTGCACTTCCACTGTCAGGGTCCCATTGTGAACCAGTTGCGGCGGGCCTT
TCTGCTCAACTGGGGGTTCTGCACAGGCAGGTATACCCCCCTGCCTCCGGCCAGCACCCTGCCCGCCGGTGAAAGCCGCT
GCCGCATTATTATGGATGGGCCGGGTTCCGAAGCCGATGTACTTAACGATATTTACTGCGGCGTGATCAACGCGGCCCGT
CAGTCCGTGCGTATAATGACGCCCTATTTTCTGCCGTCGCACGGTTTTGTGTCGGCCCTGCGTAGTGCGGCTCAGCGCGG
CGTGGATGTGCAGGTGGTGCTTCCGGCCAAGAACAACCTTTTTTACGTTCACTGGGCGCAGTTCCGCCTGCTGCCCACCC
TGCTTGAGGCGGGCGTGCGCGTCTGGTATCAGCCACCACCCTTTGCGCACACCAAGCTGCTTACTGTCGACTATTACTAC
AGCCAGATAGGTTCGGCCAATCTGGATACACGCAGCCTGCGGCTCAATTTTGAACTGAATATGGAAATATTCGACACGAG
TTTTTGCGGGGAGGTCGCGGGCTACATTGATCAGGTCATCGGCAAAAGCAGGGAAATTACGGCAGAAGAACTGAAAAACC
TGTCCCTGCCCGCGAAGCTGCGCAATGCGGGCTGCTGGATATTCTCTCCCTATCTGTAA

Upstream 100 bases:

>100_bases
CGCAACCGCATTGCGCTCAATCCGCACCATCAGGGCAGGTAGTTTGCTGGCCGCCATGCACGGCGACAGGCTGGCGTGTA
AAATTTGCAAAGGATACTGA

Downstream 100 bases:

>100_bases
GAGGGCGAAAAACAGCCAGGAGCGAATCCTGTAGGCCGTAAACTGCGAAGCGTGTGTGAAGGCCCGCCACAGCAAGGGCA
TCCGGCATATGGAAAAGCCT

Product: phospholipase D/transphosphatidylase

Products: NA

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MIIGVLETLVLIGVHVLSWVAVCHALLTKHDPRAALGWTVTALLLPLVGPLLYATFGISRAESHASRMMRQQQPLEPDYA
HPPFPELPPEDVPEYIISMERIGRRLTEQHLCSGNSLTPLHNGDEAYPAMLKAIREAKEQVFLCTYIFNAGKTATDFGNA
MAEAAERGVDVRLLVDGIGMLYSWRKPWKKLAERGVNVAMFLPPRLFPPNFSINLRNHRKMLVCDNTAFTGGMNISDDNI
SAGKTKYVQDVHFHCQGPIVNQLRRAFLLNWGFCTGRYTPLPPASTLPAGESRCRIIMDGPGSEADVLNDIYCGVINAAR
QSVRIMTPYFLPSHGFVSALRSAAQRGVDVQVVLPAKNNLFYVHWAQFRLLPTLLEAGVRVWYQPPPFAHTKLLTVDYYY
SQIGSANLDTRSLRLNFELNMEIFDTSFCGEVAGYIDQVIGKSREITAEELKNLSLPAKLRNAGCWIFSPYL

Sequences:

>Translated_472_residues
MIIGVLETLVLIGVHVLSWVAVCHALLTKHDPRAALGWTVTALLLPLVGPLLYATFGISRAESHASRMMRQQQPLEPDYA
HPPFPELPPEDVPEYIISMERIGRRLTEQHLCSGNSLTPLHNGDEAYPAMLKAIREAKEQVFLCTYIFNAGKTATDFGNA
MAEAAERGVDVRLLVDGIGMLYSWRKPWKKLAERGVNVAMFLPPRLFPPNFSINLRNHRKMLVCDNTAFTGGMNISDDNI
SAGKTKYVQDVHFHCQGPIVNQLRRAFLLNWGFCTGRYTPLPPASTLPAGESRCRIIMDGPGSEADVLNDIYCGVINAAR
QSVRIMTPYFLPSHGFVSALRSAAQRGVDVQVVLPAKNNLFYVHWAQFRLLPTLLEAGVRVWYQPPPFAHTKLLTVDYYY
SQIGSANLDTRSLRLNFELNMEIFDTSFCGEVAGYIDQVIGKSREITAEELKNLSLPAKLRNAGCWIFSPYL
>Mature_472_residues
MIIGVLETLVLIGVHVLSWVAVCHALLTKHDPRAALGWTVTALLLPLVGPLLYATFGISRAESHASRMMRQQQPLEPDYA
HPPFPELPPEDVPEYIISMERIGRRLTEQHLCSGNSLTPLHNGDEAYPAMLKAIREAKEQVFLCTYIFNAGKTATDFGNA
MAEAAERGVDVRLLVDGIGMLYSWRKPWKKLAERGVNVAMFLPPRLFPPNFSINLRNHRKMLVCDNTAFTGGMNISDDNI
SAGKTKYVQDVHFHCQGPIVNQLRRAFLLNWGFCTGRYTPLPPASTLPAGESRCRIIMDGPGSEADVLNDIYCGVINAAR
QSVRIMTPYFLPSHGFVSALRSAAQRGVDVQVVLPAKNNLFYVHWAQFRLLPTLLEAGVRVWYQPPPFAHTKLLTVDYYY
SQIGSANLDTRSLRLNFELNMEIFDTSFCGEVAGYIDQVIGKSREITAEELKNLSLPAKLRNAGCWIFSPYL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=477, Percent_Identity=28.9308176100629, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1787007, Length=336, Percent_Identity=30.952380952381, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 52748; Mature: 52748

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGVLETLVLIGVHVLSWVAVCHALLTKHDPRAALGWTVTALLLPLVGPLLYATFGISR
CEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
AESHASRMMRQQQPLEPDYAHPPFPELPPEDVPEYIISMERIGRRLTEQHLCSGNSLTPL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HNGDEAYPAMLKAIREAKEQVFLCTYIFNAGKTATDFGNAMAEAAERGVDVRLLVDGIGM
CCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHH
LYSWRKPWKKLAERGVNVAMFLPPRLFPPNFSINLRNHRKMLVCDNTAFTGGMNISDDNI
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCEEEEECCCEEECCCCCCCCCC
SAGKTKYVQDVHFHCQGPIVNQLRRAFLLNWGFCTGRYTPLPPASTLPAGESRCRIIMDG
CCCCHHHHHHHEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECC
PGSEADVLNDIYCGVINAARQSVRIMTPYFLPSHGFVSALRSAAQRGVDVQVVLPAKNNL
CCCHHHHHHHHHHHHHHHHHHCEEEECCEECCCCHHHHHHHHHHHCCCEEEEEEECCCCE
FYVHWAQFRLLPTLLEAGVRVWYQPPPFAHTKLLTVDYYYSQIGSANLDTRSLRLNFELN
EEEEHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEEHHHHHCCCCCCCCEEEEEEEEEE
MEIFDTSFCGEVAGYIDQVIGKSREITAEELKNLSLPAKLRNAGCWIFSPYL
EEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHCCCCEEECCCC
>Mature Secondary Structure
MIIGVLETLVLIGVHVLSWVAVCHALLTKHDPRAALGWTVTALLLPLVGPLLYATFGISR
CEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
AESHASRMMRQQQPLEPDYAHPPFPELPPEDVPEYIISMERIGRRLTEQHLCSGNSLTPL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HNGDEAYPAMLKAIREAKEQVFLCTYIFNAGKTATDFGNAMAEAAERGVDVRLLVDGIGM
CCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHH
LYSWRKPWKKLAERGVNVAMFLPPRLFPPNFSINLRNHRKMLVCDNTAFTGGMNISDDNI
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCEEEEECCCEEECCCCCCCCCC
SAGKTKYVQDVHFHCQGPIVNQLRRAFLLNWGFCTGRYTPLPPASTLPAGESRCRIIMDG
CCCCHHHHHHHEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECC
PGSEADVLNDIYCGVINAARQSVRIMTPYFLPSHGFVSALRSAAQRGVDVQVVLPAKNNL
CCCHHHHHHHHHHHHHHHHHHCEEEECCEECCCCHHHHHHHHHHHCCCEEEEEEECCCCE
FYVHWAQFRLLPTLLEAGVRVWYQPPPFAHTKLLTVDYYYSQIGSANLDTRSLRLNFELN
EEEEHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEEHHHHHCCCCCCCCEEEEEEEEEE
MEIFDTSFCGEVAGYIDQVIGKSREITAEELKNLSLPAKLRNAGCWIFSPYL
EEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]