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The map label for this gene is lpdA [H]

Identifier: 220905607

GI number: 220905607

Start: 2828124

End: 2829551

Strand: Direct

Name: lpdA [H]

Synonym: Ddes_2347

Alternate gene names: 220905607

Gene position: 2828124-2829551 (Clockwise)

Preceding gene: 220905606

Following gene: 220905608

Centisome position: 98.42

GC content: 64.29

Gene sequence:

>1428_bases
ATGACTGCCGAAACCAAGACCCTGACAGTCATCGGCGGCGGTCCGGGCGGCTACACGGCGGCGTTTGCGGCTGCCCGCGC
CGGCATGCGCGTAACGCTGGTGGAATGTGAATCTCTTGGGGGAACCTGTCTCAACCACGGCTGTATCCCCACCAAAACCA
TCAAGTCCTCTGCCGAGGCGCTGGAACTGGCCCAGAACGCGGCTGCGTTCGGCGTACGCATCGAAGGCGGCATACATATA
GACCCCGCGGCCGTGCTCCAGCGCAAGGAACGTGTGCGATCCATACTCTGCTCCGGCCTGGAAAAGACCTGCGCCTCCCT
GGGTATTGACCTGGTGCGAGGCCGGGGCCGCATACTGCGCGCCGGGCTGGTTGAACTGATCGGGACCGGCGGCACCCGCA
CCGTGGAAGCCGACAATATTATCATCGCCACCGGTTCACGCCCGGTAGAACTGCCCGGTCTGCCCATAGACCACACGCAT
ATCCTGAACAGTGATGACGCCCTGCGGCTGGAATCCGTGCCGTCTTCGCTCATCATTGTGGGCGGCGGTGTTATTGGCTG
TGAAATGGCCTGTATTTACCGTGCGTTCGGCGCCACCGTAACTCTTGTGGAAGGGCAGGACCGTATTCTGCCCCTGCCCT
CGGTGGATGAAGATATCAGCGCCCTTTTGCAGCGCGAGATGAAAAAGCGCCGTATCGCCTGCGAAACCGGCTGCACGCTC
ACCAATGTGACCACCGGCCCGGACGGCGTGCATGCGGCGCTGGCGGCATCGCCCTTTGCGCCTGCCGGCGCTGTTCCCCG
GCCGGAAAAAACCGTGCGGGCCGAAAAAGTGCTGGTAACCGTAGGGCGCGCGCCCTGTACAGACGGCCTCGGCCTTGAAG
AAACCGGAATTGCGGCGGACGCGCGAGGCTGGATTACGGCGGACGCGCGCATGCGCACGTCTGTGCCGCATATTTACGCC
ATCGGTGATGTGCTTGGTCCCGGCCGCGTCATGCTCGCCCACGCGGCCTCGGCGGAAGCGCTGTGCGCCGTGGCAGACTG
CCTCGGCAAGGATACGGGCCGGGATATGGATTACCGCCACATCCCTTCGGCTATCTTTACGTCGCCCGAAATAGGCTGCG
TCGGCATGAGCGAGCGGCAGGCCAGGGATGCCGGGCATGAAGTCAAAACATCTGTGGTGCAGATGCGCGAACTGGGCAAG
GCCCAGGCCATGAGCGCCCTGCCCGGCTTCTGCAAACTGGTGGCCGATGCGCGCACAGACGCTCTGCTGGGTGTGCACAT
GGCCGGTGCGCACGCCACAGACCTTGTAGCCGAGGGCGTTCTGGCCCTGCACCTTGGGGCTGCGGTTGCAGATGTAGCCG
CGGCGGTTCACGCCCACCCCACCCTTGCGGAAGCTATGGGAGAGGCCGCCCTGCGCATGCCCCAGTAA

Upstream 100 bases:

>100_bases
TTGAAAAGGTCATGCGCAAGGGCTGTCTCGTGACGGGATCGCTGGAAAATGCCTTTTCCGTGACCTATAAGCTTACCCTT
GCCGAAGACGAGGACTAGCC

Downstream 100 bases:

>100_bases
TCCACGCCCAAACGTCGCTGAACAGCGCAAGAGAACAGCAACAAAGCTCAGGAGAACAGCATGAAAGATCTGGATCAGGT
GGAATTGCCCGAAAACATCC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTAETKTLTVIGGGPGGYTAAFAAARAGMRVTLVECESLGGTCLNHGCIPTKTIKSSAEALELAQNAAAFGVRIEGGIHI
DPAAVLQRKERVRSILCSGLEKTCASLGIDLVRGRGRILRAGLVELIGTGGTRTVEADNIIIATGSRPVELPGLPIDHTH
ILNSDDALRLESVPSSLIIVGGGVIGCEMACIYRAFGATVTLVEGQDRILPLPSVDEDISALLQREMKKRRIACETGCTL
TNVTTGPDGVHAALAASPFAPAGAVPRPEKTVRAEKVLVTVGRAPCTDGLGLEETGIAADARGWITADARMRTSVPHIYA
IGDVLGPGRVMLAHAASAEALCAVADCLGKDTGRDMDYRHIPSAIFTSPEIGCVGMSERQARDAGHEVKTSVVQMRELGK
AQAMSALPGFCKLVADARTDALLGVHMAGAHATDLVAEGVLALHLGAAVADVAAAVHAHPTLAEAMGEAALRMPQ

Sequences:

>Translated_475_residues
MTAETKTLTVIGGGPGGYTAAFAAARAGMRVTLVECESLGGTCLNHGCIPTKTIKSSAEALELAQNAAAFGVRIEGGIHI
DPAAVLQRKERVRSILCSGLEKTCASLGIDLVRGRGRILRAGLVELIGTGGTRTVEADNIIIATGSRPVELPGLPIDHTH
ILNSDDALRLESVPSSLIIVGGGVIGCEMACIYRAFGATVTLVEGQDRILPLPSVDEDISALLQREMKKRRIACETGCTL
TNVTTGPDGVHAALAASPFAPAGAVPRPEKTVRAEKVLVTVGRAPCTDGLGLEETGIAADARGWITADARMRTSVPHIYA
IGDVLGPGRVMLAHAASAEALCAVADCLGKDTGRDMDYRHIPSAIFTSPEIGCVGMSERQARDAGHEVKTSVVQMRELGK
AQAMSALPGFCKLVADARTDALLGVHMAGAHATDLVAEGVLALHLGAAVADVAAAVHAHPTLAEAMGEAALRMPQ
>Mature_474_residues
TAETKTLTVIGGGPGGYTAAFAAARAGMRVTLVECESLGGTCLNHGCIPTKTIKSSAEALELAQNAAAFGVRIEGGIHID
PAAVLQRKERVRSILCSGLEKTCASLGIDLVRGRGRILRAGLVELIGTGGTRTVEADNIIIATGSRPVELPGLPIDHTHI
LNSDDALRLESVPSSLIIVGGGVIGCEMACIYRAFGATVTLVEGQDRILPLPSVDEDISALLQREMKKRRIACETGCTLT
NVTTGPDGVHAALAASPFAPAGAVPRPEKTVRAEKVLVTVGRAPCTDGLGLEETGIAADARGWITADARMRTSVPHIYAI
GDVLGPGRVMLAHAASAEALCAVADCLGKDTGRDMDYRHIPSAIFTSPEIGCVGMSERQARDAGHEVKTSVVQMRELGKA
QAMSALPGFCKLVADARTDALLGVHMAGAHATDLVAEGVLALHLGAAVADVAAAVHAHPTLAEAMGEAALRMPQ

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=470, Percent_Identity=35.3191489361702, Blast_Score=263, Evalue=4e-70,
Organism=Homo sapiens, GI50301238, Length=453, Percent_Identity=27.5938189845475, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI22035672, Length=440, Percent_Identity=29.5454545454545, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI33519430, Length=439, Percent_Identity=24.1457858769932, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI33519428, Length=439, Percent_Identity=24.1457858769932, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI33519426, Length=439, Percent_Identity=24.1457858769932, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI148277065, Length=439, Percent_Identity=24.1457858769932, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI148277071, Length=439, Percent_Identity=24.1457858769932, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI291045266, Length=437, Percent_Identity=23.7986270022883, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI291045268, Length=291, Percent_Identity=25.085910652921, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786307, Length=473, Percent_Identity=35.9408033826639, Blast_Score=273, Evalue=3e-74,
Organism=Escherichia coli, GI87082354, Length=479, Percent_Identity=27.9749478079332, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789915, Length=453, Percent_Identity=27.1523178807947, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI87081717, Length=469, Percent_Identity=26.226012793177, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32565766, Length=479, Percent_Identity=35.4906054279749, Blast_Score=260, Evalue=9e-70,
Organism=Caenorhabditis elegans, GI71983429, Length=451, Percent_Identity=25.2771618625277, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=451, Percent_Identity=25.2771618625277, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17557007, Length=490, Percent_Identity=24.6938775510204, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=444, Percent_Identity=24.5495495495495, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=474, Percent_Identity=33.9662447257384, Blast_Score=239, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6325240, Length=483, Percent_Identity=29.399585921325, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=27.1551724137931, Blast_Score=154, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21358499, Length=466, Percent_Identity=36.6952789699571, Blast_Score=271, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24640551, Length=480, Percent_Identity=27.5, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640549, Length=480, Percent_Identity=27.5, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640553, Length=480, Percent_Identity=27.5, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17737741, Length=482, Percent_Identity=27.8008298755187, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49089; Mature: 48958

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAETKTLTVIGGGPGGYTAAFAAARAGMRVTLVECESLGGTCLNHGCIPTKTIKSSAEA
CCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHCCCCCCCHHHHHHHHH
LELAQNAAAFGVRIEGGIHIDPAAVLQRKERVRSILCSGLEKTCASLGIDLVRGRGRILR
HHHHHHHHHEEEEECCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHH
AGLVELIGTGGTRTVEADNIIIATGSRPVELPGLPIDHTHILNSDDALRLESVPSSLIIV
HHHHHHHCCCCCEEEECCEEEEECCCCCEECCCCCCCCCEEECCCCCEEEECCCCEEEEE
GGGVIGCEMACIYRAFGATVTLVEGQDRILPLPSVDEDISALLQREMKKRRIACETGCTL
ECCHHHHHHHHHHHHHCCEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEE
TNVTTGPDGVHAALAASPFAPAGAVPRPEKTVRAEKVLVTVGRAPCTDGLGLEETGIAAD
EEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCC
ARGWITADARMRTSVPHIYAIGDVLGPGRVMLAHAASAEALCAVADCLGKDTGRDMDYRH
CCCEEEECCHHHCCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCHHH
IPSAIFTSPEIGCVGMSERQARDAGHEVKTSVVQMRELGKAQAMSALPGFCKLVADARTD
CCHHHHCCCCCCEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCC
ALLGVHMAGAHATDLVAEGVLALHLGAAVADVAAAVHAHPTLAEAMGEAALRMPQ
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TAETKTLTVIGGGPGGYTAAFAAARAGMRVTLVECESLGGTCLNHGCIPTKTIKSSAEA
CCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHCCCCCCCHHHHHHHHH
LELAQNAAAFGVRIEGGIHIDPAAVLQRKERVRSILCSGLEKTCASLGIDLVRGRGRILR
HHHHHHHHHEEEEECCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHH
AGLVELIGTGGTRTVEADNIIIATGSRPVELPGLPIDHTHILNSDDALRLESVPSSLIIV
HHHHHHHCCCCCEEEECCEEEEECCCCCEECCCCCCCCCEEECCCCCEEEECCCCEEEEE
GGGVIGCEMACIYRAFGATVTLVEGQDRILPLPSVDEDISALLQREMKKRRIACETGCTL
ECCHHHHHHHHHHHHHCCEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEE
TNVTTGPDGVHAALAASPFAPAGAVPRPEKTVRAEKVLVTVGRAPCTDGLGLEETGIAAD
EEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCC
ARGWITADARMRTSVPHIYAIGDVLGPGRVMLAHAASAEALCAVADCLGKDTGRDMDYRH
CCCEEEECCHHHCCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCHHH
IPSAIFTSPEIGCVGMSERQARDAGHEVKTSVVQMRELGKAQAMSALPGFCKLVADARTD
CCHHHHCCCCCCEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCC
ALLGVHMAGAHATDLVAEGVLALHLGAAVADVAAAVHAHPTLAEAMGEAALRMPQ
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339281; 1581034 [H]