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The map label for this gene is gcvH [H]

Identifier: 220905608

GI number: 220905608

Start: 2829612

End: 2830016

Strand: Direct

Name: gcvH [H]

Synonym: Ddes_2348

Alternate gene names: 220905608

Gene position: 2829612-2830016 (Clockwise)

Preceding gene: 220905607

Following gene: 220905609

Centisome position: 98.47

GC content: 60.0

Gene sequence:

>405_bases
ATGAAAGATCTGGATCAGGTGGAATTGCCCGAAAACATCCGCTACACCAGCGAGCATGTCTGGCTGCGCATGGACGGCGA
AGCGGCCCTTGTAGGCATCAGCGACTTTGCCCAGGACCAGCTCGGCGAAATCGCCTTTGTGGACCTGCCCGCCGAAGGTG
CGCATTTCAAGGCGGGCGAAGAGTTCGGCACGGTGGAATCCATCAAGTCCGTCAGTTCCCTCTACATGCCTGTTTCCGGC
ACGGTACGCACCGTAAACAGCGCCCTTGAAGATGCGCCCACCCTTGTCAATGTGGAACCGTACACCGAGGGCTGGATGAT
CTGCATCACCCCCGACAACCCCGATGACGCCGCAGCCATGACGGACGCCGCAGCCTACCGGGCGCTGCTGGCTGATAACG
CATAA

Upstream 100 bases:

>100_bases
GGAAGCTATGGGAGAGGCCGCCCTGCGCATGCCCCAGTAATCCACGCCCAAACGTCGCTGAACAGCGCAAGAGAACAGCA
ACAAAGCTCAGGAGAACAGC

Downstream 100 bases:

>100_bases
GACAGGGCCTGGAGCAGCCATGACGGACGCATCTCTGGCGCTCAGCCACCGGAAAGATCGTGCCTTTGCCCGCAAGGGCC
TGACCGTTGCCGCCATAAAT

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MKDLDQVELPENIRYTSEHVWLRMDGEAALVGISDFAQDQLGEIAFVDLPAEGAHFKAGEEFGTVESIKSVSSLYMPVSG
TVRTVNSALEDAPTLVNVEPYTEGWMICITPDNPDDAAAMTDAAAYRALLADNA

Sequences:

>Translated_134_residues
MKDLDQVELPENIRYTSEHVWLRMDGEAALVGISDFAQDQLGEIAFVDLPAEGAHFKAGEEFGTVESIKSVSSLYMPVSG
TVRTVNSALEDAPTLVNVEPYTEGWMICITPDNPDDAAAMTDAAAYRALLADNA
>Mature_134_residues
MKDLDQVELPENIRYTSEHVWLRMDGEAALVGISDFAQDQLGEIAFVDLPAEGAHFKAGEEFGTVESIKSVSSLYMPVSG
TVRTVNSALEDAPTLVNVEPYTEGWMICITPDNPDDAAAMTDAAAYRALLADNA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=113, Percent_Identity=38.9380530973451, Blast_Score=96, Evalue=6e-21,
Organism=Homo sapiens, GI89057342, Length=113, Percent_Identity=38.0530973451327, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1789271, Length=125, Percent_Identity=44, Blast_Score=113, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17507493, Length=118, Percent_Identity=35.5932203389831, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17551294, Length=92, Percent_Identity=41.304347826087, Blast_Score=92, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6319272, Length=101, Percent_Identity=40.5940594059406, Blast_Score=95, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17865652, Length=126, Percent_Identity=38.8888888888889, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14495; Mature: 14495

Theoretical pI: Translated: 3.80; Mature: 3.80

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLDQVELPENIRYTSEHVWLRMDGEAALVGISDFAQDQLGEIAFVDLPAEGAHFKAGE
CCCCCCCCCCCCCEECCCEEEEEECCCEEEEECHHHHHHHCCCEEEEEECCCCCCCCCCC
EFGTVESIKSVSSLYMPVSGTVRTVNSALEDAPTLVNVEPYTEGWMICITPDNPDDAAAM
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCCCCHHHH
TDAAAYRALLADNA
HHHHHHHHHHCCCC
>Mature Secondary Structure
MKDLDQVELPENIRYTSEHVWLRMDGEAALVGISDFAQDQLGEIAFVDLPAEGAHFKAGE
CCCCCCCCCCCCCEECCCEEEEEECCCEEEEECHHHHHHHCCCEEEEEECCCCCCCCCCC
EFGTVESIKSVSSLYMPVSGTVRTVNSALEDAPTLVNVEPYTEGWMICITPDNPDDAAAM
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCCCCHHHH
TDAAAYRALLADNA
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA