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The map label for this gene is purD [H]

Identifier: 220905583

GI number: 220905583

Start: 2792038

End: 2793327

Strand: Reverse

Name: purD [H]

Synonym: Ddes_2323

Alternate gene names: 220905583

Gene position: 2793327-2792038 (Counterclockwise)

Preceding gene: 220905584

Following gene: 220905582

Centisome position: 97.21

GC content: 61.55

Gene sequence:

>1290_bases
ATGCGCATCCTGGTAATAGGATCCGGTGGGCGGGAACACGCCCTGGTCTGGAAATTCATGCAAAGCCCCGGTGTGAGCGC
GGTTTTCGTCGCTCCCGGCAACGGCGGCACCAGCAGGGAAGGCGCGGTCAACGTGCCTGTGGCCGTGGATGATCTGGACG
GGCTGGTTGATCTGGCCCGCAGGGAAAAAATAGATCTTGTGGTTCCCGGGCCGGAACTGCCGCTGACTCTGGGCATTACC
GACCGCATGCGCGAGGCGGGCATTGCCTGTTTCGGGCCGGACAGCTACGGCGCGCGGCTTGAAGGCAGCAAGGCCTTTGC
CAAGGAGATCATGGCACGGGCCAAGGTTCCCACAGCCCAGAGCGCTGTGTTCAGCGATGTGGAAATGGCCAGAAATCATG
TCGCAAAGGTGGGCGGTCCCCTGGTGGTCAAGGCCGATGGCCTGGCTGCGGGCAAGGGCGTCATCATGGCCGCCGATTCT
CAGGAAGCCCTGCAGGCCGTCAACGACATCATGAGCGCCCGCGCCTTTGGCGCCGCAGGCGACCTTGTGGTGCTTGAGGA
ATGCCTTGTGGGCGAAGAGGCTTCTTTTCTCTGCTTGTGCGACGGCGAACGCGCCGTTCCTCTGCCTTCGGCCCAGGACC
ACAAGCGTGTTTTCGACAATGACGAAGGTCCCAATACCGGCGGCATGGGCGCGTACAGCCCGGCTCCCATTTTGCCCGAC
AACATGCTTGAAGAAATGGCCGACCTTACCGTGCGCCCCATTCTGCGCGAGCTTGCCAGGGACGGGCACCCCTTTGTGGG
CGTGCTCTATGCAGGGCTTATGATGACCGCGGACGGTCCCAAGGTGCTGGAATACAACGTGCGTTTCGGTGATCCGGAGT
GCCAGCCCATGCTCATGCGCCTCGAAGGCGATCTGCCCCGCATCATGCTCGACTGCGTGGAAGGCAGGCTCGACCCCACA
AGTATCGGCCAGAGCGACAAGACGGCCCTCGGCGTGGTCATGACGGCCAAGGGCTACCCGGGCGCGTATTCCAAGGGGCT
TGCCATTGCGGATATTGAAGAGGCCGACAGTCTGCCCGGAGTCAAGGTTTTTCATAGCGGCACGACCATCAGGGATGGCG
CGCTGGTGTCCAGTGGCGGGCGCGTGCTTTGCGTCACGGCGTTGGGGAATGATCTGGAGGACGCAAAAAAAGCCGCCTAC
GCTGCTGTGGAAAAAGTTCGTATGACTGACGGCTTTTACCGCACAGATATCGGGCAGAAGGGCATCAATCGCCTGAAAGG
CATAAAGTAG

Upstream 100 bases:

>100_bases
ACGGGCGCGGGCGGCCCACGCGCACTGCGGCGGCAGCCCGCCCCCGGTAAAGCCCCTTTATGGGGCTTTGCTTTTCATCA
GCACACGGCAAGGAGTGAGC

Downstream 100 bases:

>100_bases
CCGGGGCGTAACATAATTTCAGCCGTTGGCCCAAGGGCTTGCGGCTAAAGTTGCAACGCAGCCATATGAAAGTGAACCAT
CGCCGCGCAAAAATGCTGCG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MRILVIGSGGREHALVWKFMQSPGVSAVFVAPGNGGTSREGAVNVPVAVDDLDGLVDLARREKIDLVVPGPELPLTLGIT
DRMREAGIACFGPDSYGARLEGSKAFAKEIMARAKVPTAQSAVFSDVEMARNHVAKVGGPLVVKADGLAAGKGVIMAADS
QEALQAVNDIMSARAFGAAGDLVVLEECLVGEEASFLCLCDGERAVPLPSAQDHKRVFDNDEGPNTGGMGAYSPAPILPD
NMLEEMADLTVRPILRELARDGHPFVGVLYAGLMMTADGPKVLEYNVRFGDPECQPMLMRLEGDLPRIMLDCVEGRLDPT
SIGQSDKTALGVVMTAKGYPGAYSKGLAIADIEEADSLPGVKVFHSGTTIRDGALVSSGGRVLCVTALGNDLEDAKKAAY
AAVEKVRMTDGFYRTDIGQKGINRLKGIK

Sequences:

>Translated_429_residues
MRILVIGSGGREHALVWKFMQSPGVSAVFVAPGNGGTSREGAVNVPVAVDDLDGLVDLARREKIDLVVPGPELPLTLGIT
DRMREAGIACFGPDSYGARLEGSKAFAKEIMARAKVPTAQSAVFSDVEMARNHVAKVGGPLVVKADGLAAGKGVIMAADS
QEALQAVNDIMSARAFGAAGDLVVLEECLVGEEASFLCLCDGERAVPLPSAQDHKRVFDNDEGPNTGGMGAYSPAPILPD
NMLEEMADLTVRPILRELARDGHPFVGVLYAGLMMTADGPKVLEYNVRFGDPECQPMLMRLEGDLPRIMLDCVEGRLDPT
SIGQSDKTALGVVMTAKGYPGAYSKGLAIADIEEADSLPGVKVFHSGTTIRDGALVSSGGRVLCVTALGNDLEDAKKAAY
AAVEKVRMTDGFYRTDIGQKGINRLKGIK
>Mature_429_residues
MRILVIGSGGREHALVWKFMQSPGVSAVFVAPGNGGTSREGAVNVPVAVDDLDGLVDLARREKIDLVVPGPELPLTLGIT
DRMREAGIACFGPDSYGARLEGSKAFAKEIMARAKVPTAQSAVFSDVEMARNHVAKVGGPLVVKADGLAAGKGVIMAADS
QEALQAVNDIMSARAFGAAGDLVVLEECLVGEEASFLCLCDGERAVPLPSAQDHKRVFDNDEGPNTGGMGAYSPAPILPD
NMLEEMADLTVRPILRELARDGHPFVGVLYAGLMMTADGPKVLEYNVRFGDPECQPMLMRLEGDLPRIMLDCVEGRLDPT
SIGQSDKTALGVVMTAKGYPGAYSKGLAIADIEEADSLPGVKVFHSGTTIRDGALVSSGGRVLCVTALGNDLEDAKKAAY
AAVEKVRMTDGFYRTDIGQKGINRLKGIK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=428, Percent_Identity=46.9626168224299, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI209869995, Length=428, Percent_Identity=46.9626168224299, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI209869993, Length=428, Percent_Identity=46.9626168224299, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI28416899, Length=428, Percent_Identity=46.9626168224299, Blast_Score=394, Evalue=1e-110,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=49.8829039812646, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=40.6103286384976, Blast_Score=289, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6321203, Length=448, Percent_Identity=43.9732142857143, Blast_Score=344, Evalue=2e-95,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=42.2273781902552, Blast_Score=302, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=42.2273781902552, Blast_Score=301, Evalue=4e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45212; Mature: 45212

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVIGSGGREHALVWKFMQSPGVSAVFVAPGNGGTSREGAVNVPVAVDDLDGLVDLAR
CEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCEEECCEEECCHHHHHHHHH
REKIDLVVPGPELPLTLGITDRMREAGIACFGPDSYGARLEGSKAFAKEIMARAKVPTAQ
HCCEEEEECCCCCCEEECCHHHHHHCCEEEECCCCCCCEECCHHHHHHHHHHHHCCCCHH
SAVFSDVEMARNHVAKVGGPLVVKADGLAAGKGVIMAADSQEALQAVNDIMSARAFGAAG
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCC
DLVVLEECLVGEEASFLCLCDGERAVPLPSAQDHKRVFDNDEGPNTGGMGAYSPAPILPD
CHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCH
NMLEEMADLTVRPILRELARDGHPFVGVLYAGLMMTADGPKVLEYNVRFGDPECQPMLMR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCEEEEEEEEECCCCCCHHHEE
LEGDLPRIMLDCVEGRLDPTSIGQSDKTALGVVMTAKGYPGAYSKGLAIADIEEADSLPG
CCCCCHHHHHHHHHCCCCHHHCCCCCCCEEEEEEEECCCCCCCCCCEEEEECHHHCCCCC
VKVFHSGTTIRDGALVSSGGRVLCVTALGNDLEDAKKAAYAAVEKVRMTDGFYRTDIGQK
EEEEECCCEEECCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
GINRLKGIK
HHHHHCCCC
>Mature Secondary Structure
MRILVIGSGGREHALVWKFMQSPGVSAVFVAPGNGGTSREGAVNVPVAVDDLDGLVDLAR
CEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCEEECCEEECCHHHHHHHHH
REKIDLVVPGPELPLTLGITDRMREAGIACFGPDSYGARLEGSKAFAKEIMARAKVPTAQ
HCCEEEEECCCCCCEEECCHHHHHHCCEEEECCCCCCCEECCHHHHHHHHHHHHCCCCHH
SAVFSDVEMARNHVAKVGGPLVVKADGLAAGKGVIMAADSQEALQAVNDIMSARAFGAAG
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCC
DLVVLEECLVGEEASFLCLCDGERAVPLPSAQDHKRVFDNDEGPNTGGMGAYSPAPILPD
CHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCH
NMLEEMADLTVRPILRELARDGHPFVGVLYAGLMMTADGPKVLEYNVRFGDPECQPMLMR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCEEEEEEEEECCCCCCHHHEE
LEGDLPRIMLDCVEGRLDPTSIGQSDKTALGVVMTAKGYPGAYSKGLAIADIEEADSLPG
CCCCCHHHHHHHHHCCCCHHHCCCCCCCEEEEEEEECCCCCCCCCCEEEEECHHHCCCCC
VKVFHSGTTIRDGALVSSGGRVLCVTALGNDLEDAKKAAYAAVEKVRMTDGFYRTDIGQK
EEEEECCCEEECCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
GINRLKGIK
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]