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The map label for this gene is purN [H]

Identifier: 220905470

GI number: 220905470

Start: 2663815

End: 2664489

Strand: Direct

Name: purN [H]

Synonym: Ddes_2208

Alternate gene names: 220905470

Gene position: 2663815-2664489 (Clockwise)

Preceding gene: 220905469

Following gene: 220905485

Centisome position: 92.7

GC content: 62.67

Gene sequence:

>675_bases
ATGCCACTGAATATCGCCATACTGGCCTCGGGCAGCGGCTCCAACGCCCAGGCCATCATCGACAAGGCCGCAGCAGGCGT
TCTGGACGTGAACGTCTGCTGCATCATCTGCAACCGGCCCGGCGCGGGCGTTATCGAGCGTGCCGCCAGGGCGGGCATTG
CCTGCGTGGTGCTGGACCACAAGGCATACCCCGACCGCGAAAGCTATGACCGCGCAGTGGTGCAACACCTTCAGAAGTAC
GACGCGAGGCTTGTGGTGCTGGCGGGCTACATGCGTCTGCTCAGCCCGGTTTTTCTGGATGCGTTTTCCGGCAGGGTCAT
CAACATCCACCCCGCGCTGCTGCCGAGCTTTCCCGGTGTGCACGGGGGCGCGGACGCCCTTGAGTACGGCGTCAGGATAT
CAGGCTGCACCGTGCATTTTGTAGAAGAAAAAATGGACGGCGGCCCGGTTATCATTCAGGCCGCCGTACCCGTGAACCCC
GGCGAAGATGTTGATGACCTCATGCAGCGCATCCACGCCATGGAGCATCGCATCTATCCGCAGGCCATACAATGGCTTGC
GCAGAACCGCATCAGCGTGTGGGGCCGCGAGGTACATGTGGCCCCAGGCTCTGCCGTCGCCACTGTTCCCGACGGCCAGT
GGCTGGTGTACCCGCCGCTGGAAGAGGGTTTTTAG

Upstream 100 bases:

>100_bases
CATTTTCTTTTCAGCATGCCACATGGAGCCTCCTGATCCCAAGCAGGGTACACCAAGGCAGCGACCCCGCACAGAAAAGC
TCTAATACAACGGAAACCTC

Downstream 100 bases:

>100_bases
AAGAATTTTACTTTGCAATGCCTTGTGGGGGGAGGAACATTTTTGTAAAAGGGTCCCCCCCCCACAAAAACCTTTATTCT
TGTTTCAATCTGTTACAGGG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MPLNIAILASGSGSNAQAIIDKAAAGVLDVNVCCIICNRPGAGVIERAARAGIACVVLDHKAYPDRESYDRAVVQHLQKY
DARLVVLAGYMRLLSPVFLDAFSGRVINIHPALLPSFPGVHGGADALEYGVRISGCTVHFVEEKMDGGPVIIQAAVPVNP
GEDVDDLMQRIHAMEHRIYPQAIQWLAQNRISVWGREVHVAPGSAVATVPDGQWLVYPPLEEGF

Sequences:

>Translated_224_residues
MPLNIAILASGSGSNAQAIIDKAAAGVLDVNVCCIICNRPGAGVIERAARAGIACVVLDHKAYPDRESYDRAVVQHLQKY
DARLVVLAGYMRLLSPVFLDAFSGRVINIHPALLPSFPGVHGGADALEYGVRISGCTVHFVEEKMDGGPVIIQAAVPVNP
GEDVDDLMQRIHAMEHRIYPQAIQWLAQNRISVWGREVHVAPGSAVATVPDGQWLVYPPLEEGF
>Mature_223_residues
PLNIAILASGSGSNAQAIIDKAAAGVLDVNVCCIICNRPGAGVIERAARAGIACVVLDHKAYPDRESYDRAVVQHLQKYD
ARLVVLAGYMRLLSPVFLDAFSGRVINIHPALLPSFPGVHGGADALEYGVRISGCTVHFVEEKMDGGPVIIQAAVPVNPG
EDVDDLMQRIHAMEHRIYPQAIQWLAQNRISVWGREVHVAPGSAVATVPDGQWLVYPPLEEGF

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=189, Percent_Identity=41.2698412698413, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI209869995, Length=189, Percent_Identity=41.2698412698413, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI209869993, Length=189, Percent_Identity=41.2698412698413, Blast_Score=162, Evalue=2e-40,
Organism=Escherichia coli, GI1788846, Length=191, Percent_Identity=45.5497382198953, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1787483, Length=191, Percent_Identity=30.3664921465969, Blast_Score=96, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=41.9889502762431, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6320616, Length=198, Percent_Identity=28.2828282828283, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582400, Length=184, Percent_Identity=45.6521739130435, Blast_Score=170, Evalue=6e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 24092; Mature: 23961

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLNIAILASGSGSNAQAIIDKAAAGVLDVNVCCIICNRPGAGVIERAARAGIACVVLDH
CCEEEEEEECCCCCCHHHHHHHHHCCEEEEEEEEEEECCCCCHHHHHHHHCCEEEEEEEC
KAYPDRESYDRAVVQHLQKYDARLVVLAGYMRLLSPVFLDAFSGRVINIHPALLPSFPGV
CCCCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCCC
HGGADALEYGVRISGCTVHFVEEKMDGGPVIIQAAVPVNPGEDVDDLMQRIHAMEHRIYP
CCCHHHHHHCEEEECEEEEEEEHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCH
QAIQWLAQNRISVWGREVHVAPGSAVATVPDGQWLVYPPLEEGF
HHHHHHHHCCHHHCCCEEEECCCCEEEECCCCCEEEECCHHCCC
>Mature Secondary Structure 
PLNIAILASGSGSNAQAIIDKAAAGVLDVNVCCIICNRPGAGVIERAARAGIACVVLDH
CEEEEEEECCCCCCHHHHHHHHHCCEEEEEEEEEEECCCCCHHHHHHHHCCEEEEEEEC
KAYPDRESYDRAVVQHLQKYDARLVVLAGYMRLLSPVFLDAFSGRVINIHPALLPSFPGV
CCCCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCCC
HGGADALEYGVRISGCTVHFVEEKMDGGPVIIQAAVPVNPGEDVDDLMQRIHAMEHRIYP
CCCHHHHHHCEEEECEEEEEEEHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCH
QAIQWLAQNRISVWGREVHVAPGSAVATVPDGQWLVYPPLEEGF
HHHHHHHHCCHHHCCCEEEECCCCEEEECCCCCEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]