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The map label for this gene is hemD [H]

Identifier: 220905469

GI number: 220905469

Start: 2662111

End: 2663634

Strand: Direct

Name: hemD [H]

Synonym: Ddes_2207

Alternate gene names: 220905469

Gene position: 2662111-2663634 (Clockwise)

Preceding gene: 220905468

Following gene: 220905470

Centisome position: 92.65

GC content: 63.78

Gene sequence:

>1524_bases
ATGATGAAGGTGTATCTTATCGGCGCAGGCCCCGGCGATCCGGGCCTGCTGACCCTCAAGGGCCGCGATGCCTTGGCCGC
TGCGGATGTGGTGGTGTATGACGCCCTTGCCAATGACAGCCTGCTTGGCCACGCCAACCCCGCTGCGGAAAAGATTTATG
TGGGCAAGGTGGCGGGCAACCACGCCCTGCCCCAGCATGAAATCAATGCCCTGCTTGTGCGCAAGGCCAAAGAAGGCAAG
GTTGTGGCCCGCCTGAAAGGCGGCGACCCGTACATCTTCGGGCGCGGCGGCGAAGAAGCCGAAGAACTGGTGGCCGCCGG
AGTGCCTTTTGAAGAAATACCCGGCATCACGAGCACCATTGCGGCCCCGGCCTATGCGGGCATTCCGCTGACGCACCGCG
ACTTCGCCTCTTCCGTCACCATCATCACCGGGCATGAAAACCCTGACAAGCCCGGCTCCGTGCACAACTGGCCCGCCCTT
GCGGCCAGCGCCTCTACCCTGGTCTTTGTCATGGGCATGAAAAACCTGCCCGATATCGCCCGCAACCTGCTGGACGCGGG
CATGGCCCCCGACACTCCGGCAGCCCTTATCTATCGCGGTACAACGCCCCGACAGCGCAGCCTCGTGTCTACCCTGGCCC
AGTTGCCCGCCGCCGCCGTTGAGGCCAGATTCACCAATCCCTCGGTTATTCTTGTGGGCAAGGTGGCAAGCCTGCACAAA
ACCCTCAACTGGTTTGAGCAAAAACCGCTTCTGGGCCGCGGCGTGGTTGTCACCCGCGCCCGTGAACAGGCCAGCGGTCT
GGCCGCCAGCCTGGCCGAACTGGGCGCGGACGTGATTCAGTGCCCCACCATTGAAATCAGCCCCATGGCCGATTATGCGG
AACTGGACGACGCCCTGAACCGCCTGGCCGAATACCGCTGGATTATCTTTACGTCGGTCAACGGCGTACGCCACTTCTGG
CTGCGTCTGGCGCAAGCCGGAAAAGACAGCCGTGCCATCGGCAGCTGCAAGGTGGCCGCCATTGGTCCCGCCACTGCCGA
AGCCCTGACACGGCGCGGCATCACCCCGGACTTCATCCCCGAGCGCTACGTGGCCGAAGGCGTGCTTGAGGGTCTTCTGG
CCCGCGAAGGCGGCGACGTGAAAGACAAGCGCTTTCTCCTGCCCCGCGCGGCCAAGGCCCGCGAGGTGTTGCCCGACGAA
CTGCGCAAGGCCGGAGCCGTGGTGGACGTCATCGCGGCGTACGAAACCATTGCCGCCGCCCATCGCAAGGATGAGGTGCT
GGAACGCATCAAGGAAGGCAGGCTGGACTGCGTGACTTTCGGCTCGTCCTCCACTGTGGAAAACTTTCTTTCCCTCATCC
CGGCGGACGTGCTCAAGGCCCACCCCGAGGTACAGCTTGCCGCCATCGGCCCCATCACGGCAGACACCCTGACGGCTCAT
GGCCTGCCCTGCCACATCCAGCCCGAGGAATACACCATTCCGGCGCTGGTGGAGGCCCTGAAAAAGCACTTTGCCAGAGT
CTGA

Upstream 100 bases:

>100_bases
GGGTGGACTGGGTTTCCAAGCCCACGCCCCTGTGCCCCGCCGGGCCTTCGGCCTTTGGCGCGCGCACGCTGCTTGAACTG
GCCAGGGGAGGCGTTCTGTA

Downstream 100 bases:

>100_bases
CCCACAGGCTGGCTTTGTTACGGCGTTGCGGGGAAGGGACCCTTTTGTAAAAGAGTTCCCTCCCCACACCCCAAAACTTT
CATTTTCTTTTCAGCATGCC

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MMKVYLIGAGPGDPGLLTLKGRDALAAADVVVYDALANDSLLGHANPAAEKIYVGKVAGNHALPQHEINALLVRKAKEGK
VVARLKGGDPYIFGRGGEEAEELVAAGVPFEEIPGITSTIAAPAYAGIPLTHRDFASSVTIITGHENPDKPGSVHNWPAL
AASASTLVFVMGMKNLPDIARNLLDAGMAPDTPAALIYRGTTPRQRSLVSTLAQLPAAAVEARFTNPSVILVGKVASLHK
TLNWFEQKPLLGRGVVVTRAREQASGLAASLAELGADVIQCPTIEISPMADYAELDDALNRLAEYRWIIFTSVNGVRHFW
LRLAQAGKDSRAIGSCKVAAIGPATAEALTRRGITPDFIPERYVAEGVLEGLLAREGGDVKDKRFLLPRAAKAREVLPDE
LRKAGAVVDVIAAYETIAAAHRKDEVLERIKEGRLDCVTFGSSSTVENFLSLIPADVLKAHPEVQLAAIGPITADTLTAH
GLPCHIQPEEYTIPALVEALKKHFARV

Sequences:

>Translated_507_residues
MMKVYLIGAGPGDPGLLTLKGRDALAAADVVVYDALANDSLLGHANPAAEKIYVGKVAGNHALPQHEINALLVRKAKEGK
VVARLKGGDPYIFGRGGEEAEELVAAGVPFEEIPGITSTIAAPAYAGIPLTHRDFASSVTIITGHENPDKPGSVHNWPAL
AASASTLVFVMGMKNLPDIARNLLDAGMAPDTPAALIYRGTTPRQRSLVSTLAQLPAAAVEARFTNPSVILVGKVASLHK
TLNWFEQKPLLGRGVVVTRAREQASGLAASLAELGADVIQCPTIEISPMADYAELDDALNRLAEYRWIIFTSVNGVRHFW
LRLAQAGKDSRAIGSCKVAAIGPATAEALTRRGITPDFIPERYVAEGVLEGLLAREGGDVKDKRFLLPRAAKAREVLPDE
LRKAGAVVDVIAAYETIAAAHRKDEVLERIKEGRLDCVTFGSSSTVENFLSLIPADVLKAHPEVQLAAIGPITADTLTAH
GLPCHIQPEEYTIPALVEALKKHFARV
>Mature_507_residues
MMKVYLIGAGPGDPGLLTLKGRDALAAADVVVYDALANDSLLGHANPAAEKIYVGKVAGNHALPQHEINALLVRKAKEGK
VVARLKGGDPYIFGRGGEEAEELVAAGVPFEEIPGITSTIAAPAYAGIPLTHRDFASSVTIITGHENPDKPGSVHNWPAL
AASASTLVFVMGMKNLPDIARNLLDAGMAPDTPAALIYRGTTPRQRSLVSTLAQLPAAAVEARFTNPSVILVGKVASLHK
TLNWFEQKPLLGRGVVVTRAREQASGLAASLAELGADVIQCPTIEISPMADYAELDDALNRLAEYRWIIFTSVNGVRHFW
LRLAQAGKDSRAIGSCKVAAIGPATAEALTRRGITPDFIPERYVAEGVLEGLLAREGGDVKDKRFLLPRAAKAREVLPDE
LRKAGAVVDVIAAYETIAAAHRKDEVLERIKEGRLDCVTFGSSSTVENFLSLIPADVLKAHPEVQLAAIGPITADTLTAH
GLPCHIQPEEYTIPALVEALKKHFARV

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=243, Percent_Identity=49.7942386831276, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6322922, Length=235, Percent_Identity=34.8936170212766, Blast_Score=126, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 53938; Mature: 53938

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKVYLIGAGPGDPGLLTLKGRDALAAADVVVYDALANDSLLGHANPAAEKIYVGKVAGN
CEEEEEEECCCCCCCEEEECCCCCHHHHHHHEEHHHCCCCCCCCCCCCCCEEEEEEECCC
HALPQHEINALLVRKAKEGKVVARLKGGDPYIFGRGGEEAEELVAAGVPFEEIPGITSTI
CCCCHHHHHHHEEEECCCCCEEEEEECCCCEEECCCCCHHHHHHHCCCCHHHCCCCHHHH
AAPAYAGIPLTHRDFASSVTIITGHENPDKPGSVHNWPALAASASTLVFVMGMKNLPDIA
CCCCCCCCCCCCHHHHCCEEEEECCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCHHHH
RNLLDAGMAPDTPAALIYRGTTPRQRSLVSTLAQLPAAAVEARFTNPSVILVGKVASLHK
HHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCCEEEEECHHHHHH
TLNWFEQKPLLGRGVVVTRAREQASGLAASLAELGADVIQCPTIEISPMADYAELDDALN
HHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHCCHHHCCCCEEECCCCHHHHHHHHHH
RLAEYRWIIFTSVNGVRHFWLRLAQAGKDSRAIGSCKVAAIGPATAEALTRRGITPDFIP
HHHHCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCH
ERYVAEGVLEGLLAREGGDVKDKRFLLPRAAKAREVLPDELRKAGAVVDVIAAYETIAAA
HHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHCCHHHHHHCCHHHHHHHHHHHHHHH
HRKDEVLERIKEGRLDCVTFGSSSTVENFLSLIPADVLKAHPEVQLAAIGPITADTLTAH
HHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCHHHHHCCCCEEEEEECCCCHHHHHCC
GLPCHIQPEEYTIPALVEALKKHFARV
CCCEEECCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMKVYLIGAGPGDPGLLTLKGRDALAAADVVVYDALANDSLLGHANPAAEKIYVGKVAGN
CEEEEEEECCCCCCCEEEECCCCCHHHHHHHEEHHHCCCCCCCCCCCCCCEEEEEEECCC
HALPQHEINALLVRKAKEGKVVARLKGGDPYIFGRGGEEAEELVAAGVPFEEIPGITSTI
CCCCHHHHHHHEEEECCCCCEEEEEECCCCEEECCCCCHHHHHHHCCCCHHHCCCCHHHH
AAPAYAGIPLTHRDFASSVTIITGHENPDKPGSVHNWPALAASASTLVFVMGMKNLPDIA
CCCCCCCCCCCCHHHHCCEEEEECCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCHHHH
RNLLDAGMAPDTPAALIYRGTTPRQRSLVSTLAQLPAAAVEARFTNPSVILVGKVASLHK
HHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCCEEEEECHHHHHH
TLNWFEQKPLLGRGVVVTRAREQASGLAASLAELGADVIQCPTIEISPMADYAELDDALN
HHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHCCHHHCCCCEEECCCCHHHHHHHHHH
RLAEYRWIIFTSVNGVRHFWLRLAQAGKDSRAIGSCKVAAIGPATAEALTRRGITPDFIP
HHHHCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCH
ERYVAEGVLEGLLAREGGDVKDKRFLLPRAAKAREVLPDELRKAGAVVDVIAAYETIAAA
HHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHCCHHHHHHCCHHHHHHHHHHHHHHH
HRKDEVLERIKEGRLDCVTFGSSSTVENFLSLIPADVLKAHPEVQLAAIGPITADTLTAH
HHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCHHHHHCCCCEEEEEECCCCHHHHHCC
GLPCHIQPEEYTIPALVEALKKHFARV
CCCEEECCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]