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The map label for this gene is nudG [H]

Identifier: 220905466

GI number: 220905466

Start: 2658225

End: 2658650

Strand: Direct

Name: nudG [H]

Synonym: Ddes_2204

Alternate gene names: 220905466

Gene position: 2658225-2658650 (Clockwise)

Preceding gene: 220905465

Following gene: 220905468

Centisome position: 92.51

GC content: 59.62

Gene sequence:

>426_bases
ATGGACAACACGCAAACCATTGACGTGGCGGCGGGCATCATCTGGCGCGGCGGACACTTTCTGGCCTCGCAACGCCCCAC
CAACAAGGTTATGGAAGGCTACTGGGAATTTCCCGGCGGCAAGATAGAAGGCGATGAAAGCCCTGAAGAAGCGCTCAGGC
GCGAACTGGCTGAAGAGCTGGGCATAGGCGTACGTGAAGCCAGCTACTGGCAGTGCGTGGAGCATTGCTATGCTGACCGC
AAGCTCAACGTACGCCTGTATTTCTTTCATGTTACCGATTTTTCCGGCGAGCCGTGCCCCGCCGAAGGGCAGAACCTGCG
CTGGATCAGCCCTGACGAGGCCCCGGCGCTGGACTTTTTGCCCGCGGATTCCGGCGTGCTTGAACAGTTGCTGACCCTCG
GCAAGCTGAACGGCGCTCCTGAATGA

Upstream 100 bases:

>100_bases
GCCCCTTGACGGGCATGCTGCGGCCGGCCCGGGGTACTTGCAAAACACGCCCCAGACCCGGTGCTCCCGGATCAGTCAGG
TGTAAAGGAAAATATCCGGA

Downstream 100 bases:

>100_bases
TCGCCCTGAGAAGACCAGAACTGAAGCTCAGGGAATCTCTGTTCTCGAAGGGGTAATACACGCCCGTTACGGCGCCTGAC
AGTGCAAACAAGCTGGCGCT

Product: NUDIX hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 141

Protein sequence:

>141_residues
MDNTQTIDVAAGIIWRGGHFLASQRPTNKVMEGYWEFPGGKIEGDESPEEALRRELAEELGIGVREASYWQCVEHCYADR
KLNVRLYFFHVTDFSGEPCPAEGQNLRWISPDEAPALDFLPADSGVLEQLLTLGKLNGAPE

Sequences:

>Translated_141_residues
MDNTQTIDVAAGIIWRGGHFLASQRPTNKVMEGYWEFPGGKIEGDESPEEALRRELAEELGIGVREASYWQCVEHCYADR
KLNVRLYFFHVTDFSGEPCPAEGQNLRWISPDEAPALDFLPADSGVLEQLLTLGKLNGAPE
>Mature_141_residues
MDNTQTIDVAAGIIWRGGHFLASQRPTNKVMEGYWEFPGGKIEGDESPEEALRRELAEELGIGVREASYWQCVEHCYADR
KLNVRLYFFHVTDFSGEPCPAEGQNLRWISPDEAPALDFLPADSGVLEQLLTLGKLNGAPE

Specific function: Specific for pyrimidine substrates. Acts on 5-methyl- dCTP, CTP and dCTP in decreasing order [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=130, Percent_Identity=41.5384615384615, Blast_Score=89, Evalue=9e-20,
Organism=Escherichia coli, GI1786288, Length=130, Percent_Identity=36.9230769230769, Blast_Score=75, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.-

Molecular weight: Translated: 15753; Mature: 15753

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNTQTIDVAAGIIWRGGHFLASQRPTNKVMEGYWEFPGGKIEGDESPEEALRRELAEEL
CCCCEEEHHHHEEEECCCEEECCCCCHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHH
GIGVREASYWQCVEHCYADRKLNVRLYFFHVTDFSGEPCPAEGQNLRWISPDEAPALDFL
CCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEECCCCCCCEEEC
PADSGVLEQLLTLGKLNGAPE
CCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDNTQTIDVAAGIIWRGGHFLASQRPTNKVMEGYWEFPGGKIEGDESPEEALRRELAEEL
CCCCEEEHHHHEEEECCCEEECCCCCHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHH
GIGVREASYWQCVEHCYADRKLNVRLYFFHVTDFSGEPCPAEGQNLRWISPDEAPALDFL
CCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEECCCCCCCEEEC
PADSGVLEQLLTLGKLNGAPE
CCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 11053429 [H]