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The map label for this gene is nirB [C]

Identifier: 220905349

GI number: 220905349

Start: 2514043

End: 2515752

Strand: Direct

Name: nirB [C]

Synonym: Ddes_2087

Alternate gene names: 220905349

Gene position: 2514043-2515752 (Clockwise)

Preceding gene: 220905348

Following gene: 220905351

Centisome position: 87.49

GC content: 61.93

Gene sequence:

>1710_bases
ATGCCCGAAAAAATTCTGGTAGTGGGCGGTGTAGCCCTTGGTCCCAAGGCCGCGGCCCGTTGCAAGCGTCTCATGCCCGA
TGCGGAAATCACCCTGGTGGATGAAAACGTTTTCATTTCTTACGGCGGCTGCGGCATACCCTACTACGTCTCCGGCGAAA
TCCAGAATCTGGACGACCTGCGCTCCACTCCCTATCATACGGTGCGCGATGCCGATTTTTTCCGCGACATGAAGGGCATC
ACTGTCCGCACCCAGACCCGCGCCACTGCCATTGACCGGACGGCCAAAACCCTTGCCGTCAAAAACGTGGTTACCGGGCA
GGAAGAAAAGCTGCCCTATGACAAGCTGGTACTCGCCACGGGTGCGACCCCGCGCGTGCCGCCCGTGGAAGGCAAGGACC
TTGAAAATGTCCTCTCCCTCACCCGGCTGGAAGCCGCCGGAGCCATCCGCACAGCCTGCCAGGAAGGCAAAATCACCGAG
GCCGTCATTGTGGGCGGCGGCTTTATCGGCCTTGAGGCCGCCGTGGCCCTGGCCGACATGTGGGGCGTCAAGTGCAGCGT
AGTGGAAATGGTCGACCAGATACTGCCCGGCGTACTTTCGCATCCCGTGGCCCGCATGGCCGCCCACGACTGCGTGACCC
ATAACGTGAATGTCTACACCGGAGAAAAAGTCCTGCGCCTTGAAGGCAAGGACGGCAAGGTCTGCAAGGTCGTCACCGAC
AAACGCGAACTGGAAGCCCAGCTGGTGGTCTTTGCGGCGGGCTTCATCCCCAACGGGCAGCTTGCCGGGGATGCCGGGCT
TGACGTGGCCCCCTTCGGCGCCATTGTGGTGGACCGGCACATGCGTACTTCTGACCCTGCCATCTATGCGGGCGGCGACT
GCGTTGCCATCAAGAACATCATTACCGGCAAGCTGGGCTACCTGCCGCTGGGCAGCATGGCCAACCGCCAGGGGCGCGTC
ATCGGCACCAACCTTGCGGGCGGCGATGCCACATTCCCCGGCTATGTCGGCTCCTGGGCCGTGAAGCTCTTTGATCTTTC
CTTCTGCGGCGTGGGCCTCACCGTTGAACGCGCCCGCGCAGAAGGTTTTGACGCCTTCAGCGTAAGTGTGGAGCAGTTTG
ACCACGCCCATTTTTATCCCGAAAAGGCCATGATGACGCTGGAACTTGTGGTAGACAAAGCCACAAGCCGCGTGCTCGGC
ATGCAGGGCGCCTGCGCTGACGGCGACTCCCTCAAGGCCCGCGTGGATGCCGTGGCTGCCGCGCTGCAGTACTCGCAACC
CACGGTGGAAGATATTTCCAACCTTGAGGTGGCCTACGCCCCGCCCTTTGCCTCGGCTATGGATGTGGTCAACGTGGTGG
GCAACGTGGCCGACAACGTGCTGGCCGGACGCTTTACCCCGGTGACCGCCGACCAGTTTATGGACCTGTGGAAAAACCGC
GAAAAAAATAACATGTTTTTCATTGATGCGCGCCCCGCTGCCGCCGGACGCGCCGTGCAGGAAAAACACCCGGACTGGCA
CGCCATACCGCTTGAAGAAGTGGCTGCCCGCATCAGCGAGGTTCCCAGGGACCGCCCCGTTGCCATCATCTGCAATACGG
GCCTGCGCGCATACGACGGCCTGCTTGTGCTGGCCCGCCACGGCATCACCGATGTGGTCAATTCCACCGGCGGCATGCAG
GCGGCCATCAAGATGGGCCTGTCGGTCTAG

Upstream 100 bases:

>100_bases
AAAATATGGGCGACAGCCCCCTGTTTTTATTGCGGTGCATACATAGTGTACGTTAATAATGCTCATTTGCTCATAATACT
CACAAGTAAGGAGCGCAGAA

Downstream 100 bases:

>100_bases
GGCAACTCAAGCTTGAAACTGCCCTGACGGCTGCGCGGGCAGGAGCAAGCGCAATCTGTTTGCGCGGTCAAACGCCGTAA
CGGGCGTGTACTACACGTTG

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: NA

Alternate protein names: CoA-disulfide reductase; CoADR [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MPEKILVVGGVALGPKAAARCKRLMPDAEITLVDENVFISYGGCGIPYYVSGEIQNLDDLRSTPYHTVRDADFFRDMKGI
TVRTQTRATAIDRTAKTLAVKNVVTGQEEKLPYDKLVLATGATPRVPPVEGKDLENVLSLTRLEAAGAIRTACQEGKITE
AVIVGGGFIGLEAAVALADMWGVKCSVVEMVDQILPGVLSHPVARMAAHDCVTHNVNVYTGEKVLRLEGKDGKVCKVVTD
KRELEAQLVVFAAGFIPNGQLAGDAGLDVAPFGAIVVDRHMRTSDPAIYAGGDCVAIKNIITGKLGYLPLGSMANRQGRV
IGTNLAGGDATFPGYVGSWAVKLFDLSFCGVGLTVERARAEGFDAFSVSVEQFDHAHFYPEKAMMTLELVVDKATSRVLG
MQGACADGDSLKARVDAVAAALQYSQPTVEDISNLEVAYAPPFASAMDVVNVVGNVADNVLAGRFTPVTADQFMDLWKNR
EKNNMFFIDARPAAAGRAVQEKHPDWHAIPLEEVAARISEVPRDRPVAIICNTGLRAYDGLLVLARHGITDVVNSTGGMQ
AAIKMGLSV

Sequences:

>Translated_569_residues
MPEKILVVGGVALGPKAAARCKRLMPDAEITLVDENVFISYGGCGIPYYVSGEIQNLDDLRSTPYHTVRDADFFRDMKGI
TVRTQTRATAIDRTAKTLAVKNVVTGQEEKLPYDKLVLATGATPRVPPVEGKDLENVLSLTRLEAAGAIRTACQEGKITE
AVIVGGGFIGLEAAVALADMWGVKCSVVEMVDQILPGVLSHPVARMAAHDCVTHNVNVYTGEKVLRLEGKDGKVCKVVTD
KRELEAQLVVFAAGFIPNGQLAGDAGLDVAPFGAIVVDRHMRTSDPAIYAGGDCVAIKNIITGKLGYLPLGSMANRQGRV
IGTNLAGGDATFPGYVGSWAVKLFDLSFCGVGLTVERARAEGFDAFSVSVEQFDHAHFYPEKAMMTLELVVDKATSRVLG
MQGACADGDSLKARVDAVAAALQYSQPTVEDISNLEVAYAPPFASAMDVVNVVGNVADNVLAGRFTPVTADQFMDLWKNR
EKNNMFFIDARPAAAGRAVQEKHPDWHAIPLEEVAARISEVPRDRPVAIICNTGLRAYDGLLVLARHGITDVVNSTGGMQ
AAIKMGLSV
>Mature_568_residues
PEKILVVGGVALGPKAAARCKRLMPDAEITLVDENVFISYGGCGIPYYVSGEIQNLDDLRSTPYHTVRDADFFRDMKGIT
VRTQTRATAIDRTAKTLAVKNVVTGQEEKLPYDKLVLATGATPRVPPVEGKDLENVLSLTRLEAAGAIRTACQEGKITEA
VIVGGGFIGLEAAVALADMWGVKCSVVEMVDQILPGVLSHPVARMAAHDCVTHNVNVYTGEKVLRLEGKDGKVCKVVTDK
RELEAQLVVFAAGFIPNGQLAGDAGLDVAPFGAIVVDRHMRTSDPAIYAGGDCVAIKNIITGKLGYLPLGSMANRQGRVI
GTNLAGGDATFPGYVGSWAVKLFDLSFCGVGLTVERARAEGFDAFSVSVEQFDHAHFYPEKAMMTLELVVDKATSRVLGM
QGACADGDSLKARVDAVAAALQYSQPTVEDISNLEVAYAPPFASAMDVVNVVGNVADNVLAGRFTPVTADQFMDLWKNRE
KNNMFFIDARPAAAGRAVQEKHPDWHAIPLEEVAARISEVPRDRPVAIICNTGLRAYDGLLVLARHGITDVVNSTGGMQA
AIKMGLSV

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=321, Percent_Identity=28.0373831775701, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI21389617, Length=321, Percent_Identity=28.0373831775701, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI226437568, Length=321, Percent_Identity=28.0373831775701, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1789765, Length=294, Percent_Identity=26.8707482993197, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789065, Length=300, Percent_Identity=29, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1788892, Length=278, Percent_Identity=28.4172661870504, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786307, Length=250, Percent_Identity=30, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=307, Percent_Identity=26.3843648208469, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24639257, Length=336, Percent_Identity=25.8928571428571, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281359715, Length=336, Percent_Identity=25.8928571428571, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281359713, Length=336, Percent_Identity=25.8928571428571, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24639250, Length=336, Percent_Identity=25.8928571428571, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18543267, Length=336, Percent_Identity=25.8928571428571, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24639252, Length=336, Percent_Identity=25.8928571428571, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585130, Length=332, Percent_Identity=24.0963855421687, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017758
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.14 [H]

Molecular weight: Translated: 60726; Mature: 60595

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEKILVVGGVALGPKAAARCKRLMPDAEITLVDENVFISYGGCGIPYYVSGEIQNLDDL
CCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCEEECCCCCCHHHH
RSTPYHTVRDADFFRDMKGITVRTQTRATAIDRTAKTLAVKNVVTGQEEKLPYDKLVLAT
HCCCCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCHHCCCHHHEEEEC
GATPRVPPVEGKDLENVLSLTRLEAAGAIRTACQEGKITEAVIVGGGFIGLEAAVALADM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHH
WGVKCSVVEMVDQILPGVLSHPVARMAAHDCVTHNVNVYTGEKVLRLEGKDGKVCKVVTD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCCEEEEECC
KRELEAQLVVFAAGFIPNGQLAGDAGLDVAPFGAIVVDRHMRTSDPAIYAGGDCVAIKNI
CHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCEEEEHHH
ITGKLGYLPLGSMANRQGRVIGTNLAGGDATFPGYVGSWAVKLFDLSFCGVGLTVERARA
HCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHEEEEEEHEECCCCEEEEHHHC
EGFDAFSVSVEQFDHAHFYPEKAMMTLELVVDKATSRVLGMQGACADGDSLKARVDAVAA
CCCCEEEEEHHHCCCCCCCCHHHHEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ALQYSQPTVEDISNLEVAYAPPFASAMDVVNVVGNVADNVLAGRFTPVTADQFMDLWKNR
HHHCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
EKNNMFFIDARPAAAGRAVQEKHPDWHAIPLEEVAARISEVPRDRPVAIICNTGLRAYDG
CCCCEEEEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHCC
LLVLARHGITDVVNSTGGMQAAIKMGLSV
EEEEECCCHHHHHHCCCCHHHHHHHCCCC
>Mature Secondary Structure 
PEKILVVGGVALGPKAAARCKRLMPDAEITLVDENVFISYGGCGIPYYVSGEIQNLDDL
CCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCEEECCCCCCHHHH
RSTPYHTVRDADFFRDMKGITVRTQTRATAIDRTAKTLAVKNVVTGQEEKLPYDKLVLAT
HCCCCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCHHCCCHHHEEEEC
GATPRVPPVEGKDLENVLSLTRLEAAGAIRTACQEGKITEAVIVGGGFIGLEAAVALADM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHH
WGVKCSVVEMVDQILPGVLSHPVARMAAHDCVTHNVNVYTGEKVLRLEGKDGKVCKVVTD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCCEEEEECC
KRELEAQLVVFAAGFIPNGQLAGDAGLDVAPFGAIVVDRHMRTSDPAIYAGGDCVAIKNI
CHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCEEEEHHH
ITGKLGYLPLGSMANRQGRVIGTNLAGGDATFPGYVGSWAVKLFDLSFCGVGLTVERARA
HCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHEEEEEEHEECCCCEEEEHHHC
EGFDAFSVSVEQFDHAHFYPEKAMMTLELVVDKATSRVLGMQGACADGDSLKARVDAVAA
CCCCEEEEEHHHCCCCCCCCHHHHEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ALQYSQPTVEDISNLEVAYAPPFASAMDVVNVVGNVADNVLAGRFTPVTADQFMDLWKNR
HHHCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
EKNNMFFIDARPAAAGRAVQEKHPDWHAIPLEEVAARISEVPRDRPVAIICNTGLRAYDG
CCCCEEEEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHCC
LLVLARHGITDVVNSTGGMQAAIKMGLSV
EEEEECCCHHHHHHCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]