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The map label for this gene is lipB

Identifier: 220905337

GI number: 220905337

Start: 2497771

End: 2498484

Strand: Direct

Name: lipB

Synonym: Ddes_2075

Alternate gene names: 220905337

Gene position: 2497771-2498484 (Clockwise)

Preceding gene: 220905336

Following gene: 220905338

Centisome position: 86.93

GC content: 59.24

Gene sequence:

>714_bases
ATGCTTGGATTCGATCTGGGCCGCACAGGCTATGAAGACGCCTTTACCCTGCAAAAACAGGTACAGGCAATGGTACAGTC
CGGCGGGGACGACATCCTGCTTCTTCTCGAGCACCCGCCCACCGTCAGCATCGGCAAAAATTCCGGTGCAGAGAATGTTC
CGCCGCACCTGCAAGACATGTGGAACGGGCATGTGGACATTGTGCACAGCACCCGGGGCGGCAATGTGACCTGCCATTTT
CCGGGCCAGCTTGTGGCCTATCCGGTTATCAGCCTGAAAAAGCGTTCCGGCGGCATCCGCGCCTATGTGCATGACCTTGA
AGAAGCCGCCATACGCATGCTGGCCCGCTTTGGCGTAACGGCAGCACGCCGTCAGGGCTTTCCCGGCGTGTGGACGGGCG
AAAGAAAGATCGCGTCGCTGGGCATCGCGGTGAGCCGCTACGTCACCATGCATGGCATGGCTCTCAACGTGGCGGAAGAC
CTGTCCCTGTTCAATATTATCTCGCCCTGCGGTCTGGAAGGCGTGGCCGCCACATCCATAGCGCGGGAAACCGCTGGCCC
GGTTCCGGACATGCCCGCCGTAAAAACAGCTTTTCTCGAAGAATTTTACCATATTTTCCAGCCCGCCGGGGACCATGCCC
AGCCTCTGCCCACCCTGCGGACCACCCAAGACCTCATGACACTGTTGCAAGACGCGCAAAGGACTGAAAAGTGA

Upstream 100 bases:

>100_bases
ATTACGGAAGAACTGGTTTTTCTTTCGCAGGATTCGACTCCCTGCGACTTGCGGTAACAGGACAGCCCGCGTAAACAAAA
CGAAAAACAGCAGAAACAAC

Downstream 100 bases:

>100_bases
CCTGCAAAACCTCCACATCCGGCTCCTGCGGCGACAGGCCGCAAAAAGCCATGCGCATGCCGCCCTGGCTGCGCCGACCA
CTGGCCTCGGACAAACGCTT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MLGFDLGRTGYEDAFTLQKQVQAMVQSGGDDILLLLEHPPTVSIGKNSGAENVPPHLQDMWNGHVDIVHSTRGGNVTCHF
PGQLVAYPVISLKKRSGGIRAYVHDLEEAAIRMLARFGVTAARRQGFPGVWTGERKIASLGIAVSRYVTMHGMALNVAED
LSLFNIISPCGLEGVAATSIARETAGPVPDMPAVKTAFLEEFYHIFQPAGDHAQPLPTLRTTQDLMTLLQDAQRTEK

Sequences:

>Translated_237_residues
MLGFDLGRTGYEDAFTLQKQVQAMVQSGGDDILLLLEHPPTVSIGKNSGAENVPPHLQDMWNGHVDIVHSTRGGNVTCHF
PGQLVAYPVISLKKRSGGIRAYVHDLEEAAIRMLARFGVTAARRQGFPGVWTGERKIASLGIAVSRYVTMHGMALNVAED
LSLFNIISPCGLEGVAATSIARETAGPVPDMPAVKTAFLEEFYHIFQPAGDHAQPLPTLRTTQDLMTLLQDAQRTEK
>Mature_237_residues
MLGFDLGRTGYEDAFTLQKQVQAMVQSGGDDILLLLEHPPTVSIGKNSGAENVPPHLQDMWNGHVDIVHSTRGGNVTCHF
PGQLVAYPVISLKKRSGGIRAYVHDLEEAAIRMLARFGVTAARRQGFPGVWTGERKIASLGIAVSRYVTMHGMALNVAED
LSLFNIISPCGLEGVAATSIARETAGPVPDMPAVKTAFLEEFYHIFQPAGDHAQPLPTLRTTQDLMTLLQDAQRTEK

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=214, Percent_Identity=33.1775700934579, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI87081767, Length=152, Percent_Identity=40.1315789473684, Blast_Score=115, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323268, Length=197, Percent_Identity=32.48730964467, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21355483, Length=209, Percent_Identity=33.9712918660287, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_DESDA (B8J3G1)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480649.1
- GeneID:   7285791
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_2075
- HOGENOM:   HBG716160
- ProtClustDB:   CLSK704928
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 25729; Mature: 25729

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 170-170

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGFDLGRTGYEDAFTLQKQVQAMVQSGGDDILLLLEHPPTVSIGKNSGAENVPPHLQDM
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCCCCCHHHHHH
WNGHVDIVHSTRGGNVTCHFPGQLVAYPVISLKKRSGGIRAYVHDLEEAAIRMLARFGVT
CCCCEEEEEECCCCEEEEECCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCH
AARRQGFPGVWTGERKIASLGIAVSRYVTMHGMALNVAEDLSLFNIISPCGLEGVAATSI
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHCCCCCCHHHHHHH
ARETAGPVPDMPAVKTAFLEEFYHIFQPAGDHAQPLPTLRTTQDLMTLLQDAQRTEK
HHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLGFDLGRTGYEDAFTLQKQVQAMVQSGGDDILLLLEHPPTVSIGKNSGAENVPPHLQDM
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCCCCCHHHHHH
WNGHVDIVHSTRGGNVTCHFPGQLVAYPVISLKKRSGGIRAYVHDLEEAAIRMLARFGVT
CCCCEEEEEECCCCEEEEECCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCH
AARRQGFPGVWTGERKIASLGIAVSRYVTMHGMALNVAEDLSLFNIISPCGLEGVAATSI
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHCCCCCCHHHHHHH
ARETAGPVPDMPAVKTAFLEEFYHIFQPAGDHAQPLPTLRTTQDLMTLLQDAQRTEK
HHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA