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The map label for this gene is dut [H]

Identifier: 220905219

GI number: 220905219

Start: 2360702

End: 2361397

Strand: Direct

Name: dut [H]

Synonym: Ddes_1957

Alternate gene names: 220905219

Gene position: 2360702-2361397 (Clockwise)

Preceding gene: 220905218

Following gene: 220905220

Centisome position: 82.16

GC content: 65.95

Gene sequence:

>696_bases
ATGGGCAAAGCCTTTGCGCGGCGCGGCACAGCGCTGCTGCCGCGCCAGCAGTTTTTACCGCTGCCTGCCCGCCACGCGGC
TTCCAGCAGGACCGGCCGGTCCGCAACTCTTGCCCCCGGCGGCAAGCTGCGCTACTACTCGCCAATGAGCAATACACAAA
ACAATACATTTACCCAGAGCGCTTCCGGCATGCTGGATGAGGTGATGGGCATGCCTGGTACCCGTGTCGCAGACGTGGCC
CTGGCCTTTGTACGCCCCGGCGCCCGAGAATGTTACGCCCGGGACAGTAAGGACTTTCTTGCTCCGGCCACCAGCCGGTC
TGCCGGTTTCGACCTGCGGGCCTGCCTTGACACGGCCGAGGCGGTCATTGCCCCCGGGCAGCGCCTCAAGGTCGGCACGG
GCATTGCCGTGCAGCCGCAGGCCGCAGACGTGGCGGGCTTTGTTTATTCCCGCAGCGGCCTTGGCGCGCGTGACGGCCTG
ACCGTGGCTCAGGGCGTGGGGGTCATTGACCCTGACTATACCGGAGAAATTCTGGTGGTTCTTCTGAACACCTCCGGGCA
GGAGCGTCGCATCGCCAATGGCGAGCGCGTGGCCCAGCTCATCTTTCAGCCCTTTATACGCCCCCGCTGGCACGAGGTGG
AGGAACTGGCCCCCACCGGGCGTGGTTCCGGCGGCTTCGGGCACACAGGGCGCTGA

Upstream 100 bases:

>100_bases
CTGAAAAGCAGCCTGTGCAAAACCCGCCCCGATGACCGCAAAAAAAGATTCCCGCAGACCATGCGGGAATCTTTTTTCTG
TCACAACGCAAATCCGGCAT

Downstream 100 bases:

>100_bases
CAGCGCCGCGCCCCTGCGGCGCGTCCAAACCAACCATCTGTTTATCGAGAAAAGGAGTCCGTCCATGTCACAAGCCTTTG
CTGCCGTCAAAGCCGGGGAA

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase Dut

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MGKAFARRGTALLPRQQFLPLPARHAASSRTGRSATLAPGGKLRYYSPMSNTQNNTFTQSASGMLDEVMGMPGTRVADVA
LAFVRPGARECYARDSKDFLAPATSRSAGFDLRACLDTAEAVIAPGQRLKVGTGIAVQPQAADVAGFVYSRSGLGARDGL
TVAQGVGVIDPDYTGEILVVLLNTSGQERRIANGERVAQLIFQPFIRPRWHEVEELAPTGRGSGGFGHTGR

Sequences:

>Translated_231_residues
MGKAFARRGTALLPRQQFLPLPARHAASSRTGRSATLAPGGKLRYYSPMSNTQNNTFTQSASGMLDEVMGMPGTRVADVA
LAFVRPGARECYARDSKDFLAPATSRSAGFDLRACLDTAEAVIAPGQRLKVGTGIAVQPQAADVAGFVYSRSGLGARDGL
TVAQGVGVIDPDYTGEILVVLLNTSGQERRIANGERVAQLIFQPFIRPRWHEVEELAPTGRGSGGFGHTGR
>Mature_230_residues
GKAFARRGTALLPRQQFLPLPARHAASSRTGRSATLAPGGKLRYYSPMSNTQNNTFTQSASGMLDEVMGMPGTRVADVAL
AFVRPGARECYARDSKDFLAPATSRSAGFDLRACLDTAEAVIAPGQRLKVGTGIAVQPQAADVAGFVYSRSGLGARDGLT
VAQGVGVIDPDYTGEILVVLLNTSGQERRIANGERVAQLIFQPFIRPRWHEVEELAPTGRGSGGFGHTGR

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI4503423, Length=142, Percent_Identity=39.4366197183099, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI70906441, Length=142, Percent_Identity=39.4366197183099, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI70906444, Length=142, Percent_Identity=39.4366197183099, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1790071, Length=140, Percent_Identity=42.8571428571429, Blast_Score=108, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71988561, Length=126, Percent_Identity=39.6825396825397, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319729, Length=129, Percent_Identity=37.984496124031, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921126, Length=172, Percent_Identity=34.3023255813954, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583610, Length=143, Percent_Identity=35.6643356643357, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 24517; Mature: 24386

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKAFARRGTALLPRQQFLPLPARHAASSRTGRSATLAPGGKLRYYSPMSNTQNNTFTQS
CCCCHHHCCCCCCCHHHHCCCCHHHHCCCCCCCEEEECCCCCEEEECCCCCCCCCCCHHH
ASGMLDEVMGMPGTRVADVALAFVRPGARECYARDSKDFLAPATSRSAGFDLRACLDTAE
HHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHH
AVIAPGQRLKVGTGIAVQPQAADVAGFVYSRSGLGARDGLTVAQGVGVIDPDYTGEILVV
HHHCCCCEEEECCCEEECCCHHHHHHHEEECCCCCCCCCCHHHHCCCCCCCCCCCEEEEE
LLNTSGQERRIANGERVAQLIFQPFIRPRWHEVEELAPTGRGSGGFGHTGR
EECCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GKAFARRGTALLPRQQFLPLPARHAASSRTGRSATLAPGGKLRYYSPMSNTQNNTFTQS
CCCHHHCCCCCCCHHHHCCCCHHHHCCCCCCCEEEECCCCCEEEECCCCCCCCCCCHHH
ASGMLDEVMGMPGTRVADVALAFVRPGARECYARDSKDFLAPATSRSAGFDLRACLDTAE
HHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHH
AVIAPGQRLKVGTGIAVQPQAADVAGFVYSRSGLGARDGLTVAQGVGVIDPDYTGEILVV
HHHCCCCEEEECCCEEECCCHHHHHHHEEECCCCCCCCCCHHHHCCCCCCCCCCCEEEEE
LLNTSGQERRIANGERVAQLIFQPFIRPRWHEVEELAPTGRGSGGFGHTGR
EECCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA