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The map label for this gene is crh [H]

Identifier: 220905058

GI number: 220905058

Start: 2167937

End: 2168335

Strand: Direct

Name: crh [H]

Synonym: Ddes_1795

Alternate gene names: 220905058

Gene position: 2167937-2168335 (Clockwise)

Preceding gene: 220905057

Following gene: 220905059

Centisome position: 75.45

GC content: 60.15

Gene sequence:

>399_bases
ATGTGCCTGACCGGAAAAACGAAAAAGATTACATCAGGCGAAGCCGCCGTGCTGCGCCGTCACGACCAACACACCTCGTC
CGGCCCCATGGCCCGAGAAGGTGAGGAAATGGAAGAAACCATTGAAGAAACGCCACGCGGGCTGTCCCTGAAGCTTTGCC
TGGGCCTGCGCAACGGCCTGCATGCCCGGCCGGCGGCGCGTCTGGCGCAAGAAGCCCAGCGCTATGCCTCAACCATACAG
CTTATCAGCGATACCGGCGAAGTTGACGCTAAAAGCATGCTTGATATTCTTTCTCTGGCCCAGCCCGCCAATGCGGAACT
GACCCTTCTGGCGCAGGGGCAGGATGCCCGCGAGGCCCTTTACGGCTTGGCCCGCCTTCTTAACACCATGCAGGACTGA

Upstream 100 bases:

>100_bases
TCATGGATGGTAGCCCGGCTGCGGCTGCCGCGCATCCTGCTGTGGGCCATGACTCTGGGCGCCCTTATTCTCATGGACGT
TGACCTGATAGGTATGTAGT

Downstream 100 bases:

>100_bases
CATGGCCCGCGCGGTACTTTTCGGTACTCCCGTTTCTCCCGGCATTGCCATTGGCCGGGCGCGCTTCATGCACAAGCTGC
GGCAGGATGAAGAACGGCAT

Product: phosphocarrier, HPr family

Products: D-sorbitol 6-phosphate [Cytoplasm]; pyruvate; alpha,alpha-trehalose 6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; D-glucosamine-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; galactitol-1-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm]; fructose-1-phosphate [Cytoplasm]; glucose-6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm]; salicin-6-phosphate [Cytoplasm]; arbutin-6-phosphate [Cytoplasm] [C]

Alternate protein names: Catabolite repression HPr [H]

Number of amino acids: Translated: 132; Mature: 132

Protein sequence:

>132_residues
MCLTGKTKKITSGEAAVLRRHDQHTSSGPMAREGEEMEETIEETPRGLSLKLCLGLRNGLHARPAARLAQEAQRYASTIQ
LISDTGEVDAKSMLDILSLAQPANAELTLLAQGQDAREALYGLARLLNTMQD

Sequences:

>Translated_132_residues
MCLTGKTKKITSGEAAVLRRHDQHTSSGPMAREGEEMEETIEETPRGLSLKLCLGLRNGLHARPAARLAQEAQRYASTIQ
LISDTGEVDAKSMLDILSLAQPANAELTLLAQGQDAREALYGLARLLNTMQD
>Mature_132_residues
MCLTGKTKKITSGEAAVLRRHDQHTSSGPMAREGEEMEETIEETPRGLSLKLCLGLRNGLHARPAARLAQEAQRYASTIQ
LISDTGEVDAKSMLDILSLAQPANAELTLLAQGQDAREALYGLARLLNTMQD

Specific function: Along with seryl-phosphorylated HPr, phosphorylated crh is implicated in carbon catabolite repression (CCR) of levanase, inositol dehydrogenase, and beta-xylosidase. It seems to exert its effect on CCR by interacting with CcpA [H]

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain [H]

Homologues:

None

Paralogues:

None

Copy number: 4180 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 2100 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 3235 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr [H]

EC number: NA

Molecular weight: Translated: 14331; Mature: 14331

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00369 PTS_HPR_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCLTGKTKKITSGEAAVLRRHDQHTSSGPMAREGEEMEETIEETPRGLSLKLCLGLRNGL
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCC
HARPAARLAQEAQRYASTIQLISDTGEVDAKSMLDILSLAQPANAELTLLAQGQDAREAL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHH
YGLARLLNTMQD
HHHHHHHHHHCC
>Mature Secondary Structure
MCLTGKTKKITSGEAAVLRRHDQHTSSGPMAREGEEMEETIEETPRGLSLKLCLGLRNGL
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCC
HARPAARLAQEAQRYASTIQLISDTGEVDAKSMLDILSLAQPANAELTLLAQGQDAREAL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHH
YGLARLLNTMQD
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sorbitol [Periplasm]; phosphoenolpyruvate; trehalose [Periplasm]; mannitol [Periplasm]; glucosamine [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; galactitol [Periplasm]; fructose [Periplasm]; beta-D-glucose [Periplasm]; diacetylchitobiose [Periplasm]; cellobiose [Periplasm]; salicin [Periplasm]; arbutin [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + sorbitol [Periplasm] = D-sorbitol 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + trehalose [Periplasm] = alpha,alpha-trehalose 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + mannitol [Periplasm] = mannitol-1-phosp

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 9237995; 11916384 [H]